##gff-version 3
##sequence-region BBa_K1529080 1 3056
BBa_K1529080	.	ribosome_entry_site	2121	2132	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1529080	.	conserved_region	2125	2128	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1529080	.	CDS	2139	2894	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_K1529080	.	point_mutation	2630	2630	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K1529080	.	engineered_region	2139	2894	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K1529080	.	engineered_region	2139	2140	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K1529080	.	CDS	2139	2888	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K1529080	.	point_mutation	2312	2312	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K1529080	.	engineered_tag	2895	2919	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K1529080	.	promoter	2059	2112	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K1529080	.	promoter	2078	2083	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1529080	.	non_covalent_binding_site	2084	2102	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1529080	.	engineered_region	2059	2112	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1529080	.	non_covalent_binding_site	2059	2077	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1529080	.	promoter	2101	2106	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1529080	.	engineered_region	1	2050	.	+	0	ID=BBa_K731030;Name=BBa_K731030
BBa_K1529080	.	promoter	1	1203	.	+	0	ID=BBa_K731201;Name=BBa_K731201;Parent=BBa_K731030
BBa_K1529080	.	CDS	1	1080	.	+	0	ID=annotation2179313;Name=araC;Parent=BBa_K731201
BBa_K1529080	.	promoter	1080	1203	.	+	0	ID=annotation2179311;Name=pBAD;Parent=BBa_K731201
BBa_K1529080	.	promoter	1043	1114	.	+	0	ID=annotation2179312;Name=PC;Parent=BBa_K731201
BBa_K1529080	.	CDS	1212	2050	.	+	0	ID=BBa_K731010;Name=BBa_K731010;Parent=BBa_K731030
BBa_K1529080	.	stop_codon	2045	2050	.	+	0	ID=annotation2180205;Name=Stop;Parent=BBa_K731010
BBa_K1529080	.	start_codon	1226	1228	.	+	0	ID=annotation2180204;Name=Start;Parent=BBa_K731010
BBa_K1529080	.	sequence_feature	1218	1225	.	+	0	ID=annotation2180202;Name=Spacer;Parent=BBa_K731010
BBa_K1529080	.	ribosome_entry_site	1212	1217	.	+	0	ID=annotation2180201;Name=RBS;Parent=BBa_K731010
BBa_K1529080	.	terminator	2928	3056	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1529080	.	terminator	2928	3007	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1529080	.	stem_loop	2939	2982	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1529080	.	engineered_region	2928	3007	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1529080	.	terminator	3016	3056	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1529080	.	engineered_region	3016	3056	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1529080	.	stop_codon	3049	3049	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1529080	.	polyA_site	3043	3056	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1529080	.	stem_loop	3023	3042	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
>BBa_K1529080
ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacgaaactcgctcgggctggccccggtgcat
tttttaaatactcgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtag
tgctcaaaagcagcttcgcctgactaatgcgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaa
aagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtga
tcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgcc
gcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttg
cccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaacccgtattggcaaatattgacggccag
ttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgac
cgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcagacaaattctcgtccctgatttttcac
caccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataa
ccgttggcctcaatcggcgttaaacccgccaccagatgggcgttaaacgagtatcccggcagcaggggatcattttgcg
cttcagccatacttttcatactcccaccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactg
cgtcttttactggctcttctcgctaacccaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaa
gccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacac
tttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttc
tccatacccgtttttttgtactagagaggaggtttttattatgtcgtgtgaagaactggaaattgtctggaacaatatt
aaagccgaagccagaacgctggcggactgtgagccaatgctggccagtttttaccacgcgacgctactcaagcacgaaa
accttggcagtgcactgagctacatgctggcgaacaagctgtcatcgccaattatgcctgctattgctatccgtgaagt
ggtggaagaagcctacgccgctgacccggaaatgatcgcctctgcggcctgtgatattcaggcggtgcgtacccgcgac
ccggcagtcgataaatactcaaccccgttgttatacctgaagggttttcatgccttgcaggcctatcgcatcggtcact
ggttgtggaatcaggggcgtcgcgcactggcaatctttctgcaaaaccaggtttctgtgacgttccaggtcgatattca
cccggcagcaaaaattggtcgcggtatcatgcttgaccacgcgacaggcatcgtcgttggtgaaacggcggtgattgaa
aacgacgtatcgattctgcaatctgtgacgcttggcggtacgggtaaatctggtggtgaccgtcacccgaaaattcgtg
aaggtgtgatgattggcgcgggcgcgaaaatcctcggcaatattgaagttgggcgcggcgcgaagattggcgcaggttc
cgtggtgctgcaaccggtgccgccgcataccaccgccgctggcgttccggctcgtattgtcggtaaaccagacagcgat
aagccatcaatggatatcgaccagcatttcaacggtattaaccatacatttgagtatggggatgggatctaataatact
agagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaat
actagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatat
taatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatg
gttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaat
atgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataa
aaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaac
aatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatga
acataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgattt
aaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgc
agtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttcta
aagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactact
agagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacg
ctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata