BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1529100 1 BBa_K1529100 rbs_ppk 2014-09-11T11:00:00Z 2015-05-08T01:10:50Z 1 1 false false _1911_ 0 22687 9 Not in stock false 1 false Riku Shinohara component2383354 1 BBa_B0034 component2383356 1 BBa_K1217002 annotation2383354 1 BBa_B0034 range2383354 1 1 12 annotation2383356 1 BBa_K1217002 range2383356 1 19 2070 BBa_K1217002 1 BBa_K1217002 ppk1(K.oralis) 2013-09-14T11:00:00Z 2015-05-08T01:09:42Z It is from Kingella oralis (ATCC 51147) genomic DNA Coding sequence of polyphosphate kinase (ppk1)(E.C. 2.7.4.1) from Kingella oralis (ATCC 51147), ppk1 catalyzes the formation of polyphosphate from ATP. false false _1531_ 0 13531 9 Not in stock false We choose to amplify ppk1 gene from Tannerella forsythia (ATCC 43037) since the sequence does not contain any of the illegal restriction sites. false Ma Tsz Shan annotation2343057 1 ppk1 range2343057 1 1 2052 BBa_K1217002_sequence 1 atgccgcaatctgctcacattctttgccgcgaactgtcgctgttggcgtttaaccgccgcgtgttggcgcaagcgcaagacccgcgcgtgccgctgttggaacgactgcgctttttgtgcatcgtgtcgtccaatctggacgaattttttgaagtgcgcatggcgtatttgcgccgtgaacaacgcgccaaccccacgcaagtgttccaatcgggacaaacgcccagcgaaaccatcacgctggtggcgcaagaagcgcacaagattattgaagagcaatatcaaatatttaaccaagaattgctgcccgcgcttgccgaggaaaatattgtgttttaccgccgcaagcagtggacaagcgcgcagcgcgaatggattgaagattatttcaaccgcgagctgctgccgattctcacgccgattggcttggatgcctcgcatccgtttccgcgtccgctgaataaatcgttgaattttgtggtggaattggatggcaaagatgcgtttggacgcgcttcgggcatggcaatcgtgcaagcgccgcgcattttgccgcgtgcgattcggctgcctgaaaatatttgcgaaggcaaagacggctttgtgtttttgtcgtccattgtgcatgagtatgtgtacaagctgtttcgcggcatgacggtgaaaggctgccatcaatttcgcctaacgcgcgatagcgatttgacggtggaaaacgatgatttgagcaacctgcgcacggcggtgcaaaatgaattgcgcgaccgcgattttggcgacggagtgcggctggaaattgccgaccaatgtcctgattatattagccaatttttgctggaacagtttaagctcacgcgcaatgagttgtatcaagtggaaggtcctgtgaatttggtgcggctgatgtcggtgcccgatatggtgaaccgtcccgatttgaaattcgcgccgttcacgccgcaatatccgcgcgcgttgcgcaaatcggcatcgctgttggacgaaatcgctaagggcgatattttgctgcaccatccgtatcaatcgtttgatccgattgtgaaatttatcaacgatgccacgcgcgatcctgatgtggtggcgattaagatgacgatttaccgcacgggtagccattccgagctggcgcgcgctttgatggcggcggcgctggcgggcaagcaagttaccgtggtggtggaattgatggcgcgttttgacgaagcgaataatgtgtcgtgggcaaccaagctggaagacgcgggcgcgcacgtggtttatggcgtgtttggctacaaggtgcacgccaaaatggcgttggtgattcgccgcgaacaaggcagcctgaaaacctatgcgcatctgggcacgggcaattatcaccaaggcacatcgcgcatctacaccgatttcggcttgctcaccgccaacacggcgataacgcgcgacatcaacacgctatttatggaaatcaccggcttggggcaggcgaacaagctgaaaaaaatctaccaaagcccgttcacgctgcacaaaatgattatggacagcatcgcgcgcgaaaccgaaaacgcgaaaaacggcaagcctgcgcggattatcgccaagctcaattcgctgattgagccgcaagtgatagaggcgttgtatgccgccagcgcggcgggggtgcagattgatttgattgtgcgtggcatgtgcgctttgcgcccacaagtggcggggctgtcggaaaacatccgcgtgcgctccattattgggcggctgctggaacacgcgcgggtttattatttttacaacgatggcgcggaaaacgtgtttatttccagcgcggattggatggggcgcaattttttcagccgcattgaaacctgcacgccgattgaatcgcccgcgctcaaacagcgcgtgatagaagagggcttactgctggcgttgcgcgacaacgtgaaggcttggcagatgcacggcgacggcagctacacgcggattgtggcggacggcgacgaggtgggcattcaagaaacgctgttggcgcgatatacggaatga BBa_B0034_sequence 1 aaagaggagaaa BBa_K1529100_sequence 1 aaagaggagaaatactagatgccgcaatctgctcacattctttgccgcgaactgtcgctgttggcgtttaaccgccgcgtgttggcgcaagcgcaagacccgcgcgtgccgctgttggaacgactgcgctttttgtgcatcgtgtcgtccaatctggacgaattttttgaagtgcgcatggcgtatttgcgccgtgaacaacgcgccaaccccacgcaagtgttccaatcgggacaaacgcccagcgaaaccatcacgctggtggcgcaagaagcgcacaagattattgaagagcaatatcaaatatttaaccaagaattgctgcccgcgcttgccgaggaaaatattgtgttttaccgccgcaagcagtggacaagcgcgcagcgcgaatggattgaagattatttcaaccgcgagctgctgccgattctcacgccgattggcttggatgcctcgcatccgtttccgcgtccgctgaataaatcgttgaattttgtggtggaattggatggcaaagatgcgtttggacgcgcttcgggcatggcaatcgtgcaagcgccgcgcattttgccgcgtgcgattcggctgcctgaaaatatttgcgaaggcaaagacggctttgtgtttttgtcgtccattgtgcatgagtatgtgtacaagctgtttcgcggcatgacggtgaaaggctgccatcaatttcgcctaacgcgcgatagcgatttgacggtggaaaacgatgatttgagcaacctgcgcacggcggtgcaaaatgaattgcgcgaccgcgattttggcgacggagtgcggctggaaattgccgaccaatgtcctgattatattagccaatttttgctggaacagtttaagctcacgcgcaatgagttgtatcaagtggaaggtcctgtgaatttggtgcggctgatgtcggtgcccgatatggtgaaccgtcccgatttgaaattcgcgccgttcacgccgcaatatccgcgcgcgttgcgcaaatcggcatcgctgttggacgaaatcgctaagggcgatattttgctgcaccatccgtatcaatcgtttgatccgattgtgaaatttatcaacgatgccacgcgcgatcctgatgtggtggcgattaagatgacgatttaccgcacgggtagccattccgagctggcgcgcgctttgatggcggcggcgctggcgggcaagcaagttaccgtggtggtggaattgatggcgcgttttgacgaagcgaataatgtgtcgtgggcaaccaagctggaagacgcgggcgcgcacgtggtttatggcgtgtttggctacaaggtgcacgccaaaatggcgttggtgattcgccgcgaacaaggcagcctgaaaacctatgcgcatctgggcacgggcaattatcaccaaggcacatcgcgcatctacaccgatttcggcttgctcaccgccaacacggcgataacgcgcgacatcaacacgctatttatggaaatcaccggcttggggcaggcgaacaagctgaaaaaaatctaccaaagcccgttcacgctgcacaaaatgattatggacagcatcgcgcgcgaaaccgaaaacgcgaaaaacggcaagcctgcgcggattatcgccaagctcaattcgctgattgagccgcaagtgatagaggcgttgtatgccgccagcgcggcgggggtgcagattgatttgattgtgcgtggcatgtgcgctttgcgcccacaagtggcggggctgtcggaaaacatccgcgtgcgctccattattgggcggctgctggaacacgcgcgggtttattatttttacaacgatggcgcggaaaacgtgtttatttccagcgcggattggatggggcgcaattttttcagccgcattgaaacctgcacgccgattgaatcgcccgcgctcaaacagcgcgtgatagaagagggcttactgctggcgttgcgcgacaacgtgaaggcttggcagatgcacggcgacggcagctacacgcggattgtggcggacggcgacgaggtgggcattcaagaaacgctgttggcgcgatatacggaatga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z