BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1529101 1 BBa_K1529101 Ptet_rbs_ppk1_ter 2014-09-11T11:00:00Z 2015-05-08T01:10:50Z 1 1 false false _1911_ 0 22687 9 Not in stock false 1 false Riku Shinohara component2383261 1 BBa_R0040 component2383270 1 BBa_K1529100 component2383268 1 BBa_B0010 annotation2383270 1 BBa_K1529100 range2383270 1 63 162 annotation2383268 1 BBa_B0010 range2383268 1 83 162 annotation2383261 1 BBa_R0040 range2383261 1 1 54 BBa_R0040 1 p(tetR) TetR repressible promoter 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210. Released HQ 2013 Sequence for pTet inverting regulator driven by the TetR protein.</P> false true _1_ 0 24 7 In stock false <P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P> true June Rhee, Connie Tao, Ty Thomson, Louis Waldman annotation1986784 1 BBa_R0040 range1986784 1 1 54 annotation1986783 1 TetR 1 range1986783 1 1 19 annotation1986787 1 -10 range1986787 1 43 48 annotation1986785 1 -35 range1986785 1 20 25 annotation1986786 1 TetR 2 range1986786 1 26 44 BBa_K1217002 1 BBa_K1217002 ppk1(K.oralis) 2013-09-14T11:00:00Z 2015-05-08T01:09:42Z It is from Kingella oralis (ATCC 51147) genomic DNA Coding sequence of polyphosphate kinase (ppk1)(E.C. 2.7.4.1) from Kingella oralis (ATCC 51147), ppk1 catalyzes the formation of polyphosphate from ATP. false false _1531_ 0 13531 9 Not in stock false We choose to amplify ppk1 gene from Tannerella forsythia (ATCC 43037) since the sequence does not contain any of the illegal restriction sites. false Ma Tsz Shan annotation2343057 1 ppk1 range2343057 1 1 2052 BBa_K1529100 1 BBa_K1529100 rbs_ppk 2014-09-11T11:00:00Z 2015-05-08T01:10:50Z 1 1 false false _1911_ 0 22687 9 Not in stock false 1 false Riku Shinohara component2383356 1 BBa_K1217002 component2383354 1 BBa_B0034 annotation2383354 1 BBa_B0034 range2383354 1 1 12 annotation2383356 1 BBa_K1217002 range2383356 1 19 2070 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1217002_sequence 1 atgccgcaatctgctcacattctttgccgcgaactgtcgctgttggcgtttaaccgccgcgtgttggcgcaagcgcaagacccgcgcgtgccgctgttggaacgactgcgctttttgtgcatcgtgtcgtccaatctggacgaattttttgaagtgcgcatggcgtatttgcgccgtgaacaacgcgccaaccccacgcaagtgttccaatcgggacaaacgcccagcgaaaccatcacgctggtggcgcaagaagcgcacaagattattgaagagcaatatcaaatatttaaccaagaattgctgcccgcgcttgccgaggaaaatattgtgttttaccgccgcaagcagtggacaagcgcgcagcgcgaatggattgaagattatttcaaccgcgagctgctgccgattctcacgccgattggcttggatgcctcgcatccgtttccgcgtccgctgaataaatcgttgaattttgtggtggaattggatggcaaagatgcgtttggacgcgcttcgggcatggcaatcgtgcaagcgccgcgcattttgccgcgtgcgattcggctgcctgaaaatatttgcgaaggcaaagacggctttgtgtttttgtcgtccattgtgcatgagtatgtgtacaagctgtttcgcggcatgacggtgaaaggctgccatcaatttcgcctaacgcgcgatagcgatttgacggtggaaaacgatgatttgagcaacctgcgcacggcggtgcaaaatgaattgcgcgaccgcgattttggcgacggagtgcggctggaaattgccgaccaatgtcctgattatattagccaatttttgctggaacagtttaagctcacgcgcaatgagttgtatcaagtggaaggtcctgtgaatttggtgcggctgatgtcggtgcccgatatggtgaaccgtcccgatttgaaattcgcgccgttcacgccgcaatatccgcgcgcgttgcgcaaatcggcatcgctgttggacgaaatcgctaagggcgatattttgctgcaccatccgtatcaatcgtttgatccgattgtgaaatttatcaacgatgccacgcgcgatcctgatgtggtggcgattaagatgacgatttaccgcacgggtagccattccgagctggcgcgcgctttgatggcggcggcgctggcgggcaagcaagttaccgtggtggtggaattgatggcgcgttttgacgaagcgaataatgtgtcgtgggcaaccaagctggaagacgcgggcgcgcacgtggtttatggcgtgtttggctacaaggtgcacgccaaaatggcgttggtgattcgccgcgaacaaggcagcctgaaaacctatgcgcatctgggcacgggcaattatcaccaaggcacatcgcgcatctacaccgatttcggcttgctcaccgccaacacggcgataacgcgcgacatcaacacgctatttatggaaatcaccggcttggggcaggcgaacaagctgaaaaaaatctaccaaagcccgttcacgctgcacaaaatgattatggacagcatcgcgcgcgaaaccgaaaacgcgaaaaacggcaagcctgcgcggattatcgccaagctcaattcgctgattgagccgcaagtgatagaggcgttgtatgccgccagcgcggcgggggtgcagattgatttgattgtgcgtggcatgtgcgctttgcgcccacaagtggcggggctgtcggaaaacatccgcgtgcgctccattattgggcggctgctggaacacgcgcgggtttattatttttacaacgatggcgcggaaaacgtgtttatttccagcgcggattggatggggcgcaattttttcagccgcattgaaacctgcacgccgattgaatcgcccgcgctcaaacagcgcgtgatagaagagggcttactgctggcgttgcgcgacaacgtgaaggcttggcagatgcacggcgacggcagctacacgcggattgtggcggacggcgacgaggtgggcattcaagaaacgctgttggcgcgatatacggaatga BBa_K1529101_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_B0034_sequence 1 aaagaggagaaa BBa_R0040_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac BBa_K1529100_sequence 1 aaagaggagaaatactagatgccgcaatctgctcacattctttgccgcgaactgtcgctgttggcgtttaaccgccgcgtgttggcgcaagcgcaagacccgcgcgtgccgctgttggaacgactgcgctttttgtgcatcgtgtcgtccaatctggacgaattttttgaagtgcgcatggcgtatttgcgccgtgaacaacgcgccaaccccacgcaagtgttccaatcgggacaaacgcccagcgaaaccatcacgctggtggcgcaagaagcgcacaagattattgaagagcaatatcaaatatttaaccaagaattgctgcccgcgcttgccgaggaaaatattgtgttttaccgccgcaagcagtggacaagcgcgcagcgcgaatggattgaagattatttcaaccgcgagctgctgccgattctcacgccgattggcttggatgcctcgcatccgtttccgcgtccgctgaataaatcgttgaattttgtggtggaattggatggcaaagatgcgtttggacgcgcttcgggcatggcaatcgtgcaagcgccgcgcattttgccgcgtgcgattcggctgcctgaaaatatttgcgaaggcaaagacggctttgtgtttttgtcgtccattgtgcatgagtatgtgtacaagctgtttcgcggcatgacggtgaaaggctgccatcaatttcgcctaacgcgcgatagcgatttgacggtggaaaacgatgatttgagcaacctgcgcacggcggtgcaaaatgaattgcgcgaccgcgattttggcgacggagtgcggctggaaattgccgaccaatgtcctgattatattagccaatttttgctggaacagtttaagctcacgcgcaatgagttgtatcaagtggaaggtcctgtgaatttggtgcggctgatgtcggtgcccgatatggtgaaccgtcccgatttgaaattcgcgccgttcacgccgcaatatccgcgcgcgttgcgcaaatcggcatcgctgttggacgaaatcgctaagggcgatattttgctgcaccatccgtatcaatcgtttgatccgattgtgaaatttatcaacgatgccacgcgcgatcctgatgtggtggcgattaagatgacgatttaccgcacgggtagccattccgagctggcgcgcgctttgatggcggcggcgctggcgggcaagcaagttaccgtggtggtggaattgatggcgcgttttgacgaagcgaataatgtgtcgtgggcaaccaagctggaagacgcgggcgcgcacgtggtttatggcgtgtttggctacaaggtgcacgccaaaatggcgttggtgattcgccgcgaacaaggcagcctgaaaacctatgcgcatctgggcacgggcaattatcaccaaggcacatcgcgcatctacaccgatttcggcttgctcaccgccaacacggcgataacgcgcgacatcaacacgctatttatggaaatcaccggcttggggcaggcgaacaagctgaaaaaaatctaccaaagcccgttcacgctgcacaaaatgattatggacagcatcgcgcgcgaaaccgaaaacgcgaaaaacggcaagcctgcgcggattatcgccaagctcaattcgctgattgagccgcaagtgatagaggcgttgtatgccgccagcgcggcgggggtgcagattgatttgattgtgcgtggcatgtgcgctttgcgcccacaagtggcggggctgtcggaaaacatccgcgtgcgctccattattgggcggctgctggaacacgcgcgggtttattatttttacaacgatggcgcggaaaacgtgtttatttccagcgcggattggatggggcgcaattttttcagccgcattgaaacctgcacgccgattgaatcgcccgcgctcaaacagcgcgtgatagaagagggcttactgctggcgttgcgcgacaacgtgaaggcttggcagatgcacggcgacggcagctacacgcggattgtggcggacggcgacgaggtgggcattcaagaaacgctgttggcgcgatatacggaatga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z