LOCUS       BBa_K1529103            2355 bp    DNA     linear       10-May-2024
DEFINITION  Plac_rbs_ppx_rbs_ppk1
ACCESSION   BBa_K1529103
VERSION     BBa_K1529103.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             229..1770
                     /label=protein
     CDS             229..277
                     /label=BBa_K1529110
     misc_feature    209..277
                     /label=BBa_K1529111
     misc_feature    290..293
                     /label=conserved
     RBS             286..297
                     /label=BBa_B0034
     CDS             304..2355
                     /label=ppk1
     CDS             304..2355
                     /label=BBa_K1217002
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagagaa tatataatat
      241 atatatatat attattaatt atatatatat atatatatac tagagaaaga ggagaaatac
      301 tagatgccgc aatctgctca cattctttgc cgcgaactgt cgctgttggc gtttaaccgc
      361 cgcgtgttgg cgcaagcgca agacccgcgc gtgccgctgt tggaacgact gcgctttttg
      421 tgcatcgtgt cgtccaatct ggacgaattt tttgaagtgc gcatggcgta tttgcgccgt
      481 gaacaacgcg ccaaccccac gcaagtgttc caatcgggac aaacgcccag cgaaaccatc
      541 acgctggtgg cgcaagaagc gcacaagatt attgaagagc aatatcaaat atttaaccaa
      601 gaattgctgc ccgcgcttgc cgaggaaaat attgtgtttt accgccgcaa gcagtggaca
      661 agcgcgcagc gcgaatggat tgaagattat ttcaaccgcg agctgctgcc gattctcacg
      721 ccgattggct tggatgcctc gcatccgttt ccgcgtccgc tgaataaatc gttgaatttt
      781 gtggtggaat tggatggcaa agatgcgttt ggacgcgctt cgggcatggc aatcgtgcaa
      841 gcgccgcgca ttttgccgcg tgcgattcgg ctgcctgaaa atatttgcga aggcaaagac
      901 ggctttgtgt ttttgtcgtc cattgtgcat gagtatgtgt acaagctgtt tcgcggcatg
      961 acggtgaaag gctgccatca atttcgccta acgcgcgata gcgatttgac ggtggaaaac
     1021 gatgatttga gcaacctgcg cacggcggtg caaaatgaat tgcgcgaccg cgattttggc
     1081 gacggagtgc ggctggaaat tgccgaccaa tgtcctgatt atattagcca atttttgctg
     1141 gaacagttta agctcacgcg caatgagttg tatcaagtgg aaggtcctgt gaatttggtg
     1201 cggctgatgt cggtgcccga tatggtgaac cgtcccgatt tgaaattcgc gccgttcacg
     1261 ccgcaatatc cgcgcgcgtt gcgcaaatcg gcatcgctgt tggacgaaat cgctaagggc
     1321 gatattttgc tgcaccatcc gtatcaatcg tttgatccga ttgtgaaatt tatcaacgat
     1381 gccacgcgcg atcctgatgt ggtggcgatt aagatgacga tttaccgcac gggtagccat
     1441 tccgagctgg cgcgcgcttt gatggcggcg gcgctggcgg gcaagcaagt taccgtggtg
     1501 gtggaattga tggcgcgttt tgacgaagcg aataatgtgt cgtgggcaac caagctggaa
     1561 gacgcgggcg cgcacgtggt ttatggcgtg tttggctaca aggtgcacgc caaaatggcg
     1621 ttggtgattc gccgcgaaca aggcagcctg aaaacctatg cgcatctggg cacgggcaat
     1681 tatcaccaag gcacatcgcg catctacacc gatttcggct tgctcaccgc caacacggcg
     1741 ataacgcgcg acatcaacac gctatttatg gaaatcaccg gcttggggca ggcgaacaag
     1801 ctgaaaaaaa tctaccaaag cccgttcacg ctgcacaaaa tgattatgga cagcatcgcg
     1861 cgcgaaaccg aaaacgcgaa aaacggcaag cctgcgcgga ttatcgccaa gctcaattcg
     1921 ctgattgagc cgcaagtgat agaggcgttg tatgccgcca gcgcggcggg ggtgcagatt
     1981 gatttgattg tgcgtggcat gtgcgctttg cgcccacaag tggcggggct gtcggaaaac
     2041 atccgcgtgc gctccattat tgggcggctg ctggaacacg cgcgggttta ttatttttac
     2101 aacgatggcg cggaaaacgt gtttatttcc agcgcggatt ggatggggcg caattttttc
     2161 agccgcattg aaacctgcac gccgattgaa tcgcccgcgc tcaaacagcg cgtgatagaa
     2221 gagggcttac tgctggcgtt gcgcgacaac gtgaaggctt ggcagatgca cggcgacggc
     2281 agctacacgc ggattgtggc ggacggcgac gaggtgggca ttcaagaaac gctgttggcg
     2341 cgatatacgg aatga
//