BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1529103 1 BBa_K1529103 Plac_rbs_ppx_rbs_ppk1 2014-09-14T11:00:00Z 2015-05-08T01:10:50Z 1 1 false false _1911_ 0 22685 9 Not in stock false 1 false Shoko Suzuki component2383532 1 BBa_R0010 component2383546 1 BBa_K1217002 component2383544 1 BBa_B0034 component2383542 1 BBa_K1529111 annotation2383546 1 BBa_K1217002 range2383546 1 304 2355 annotation2383542 1 BBa_K1529111 range2383542 1 209 277 annotation2383532 1 BBa_R0010 range2383532 1 1 200 annotation2383544 1 BBa_B0034 range2383544 1 286 297 BBa_K1217002 1 BBa_K1217002 ppk1(K.oralis) 2013-09-14T11:00:00Z 2015-05-08T01:09:42Z It is from Kingella oralis (ATCC 51147) genomic DNA Coding sequence of polyphosphate kinase (ppk1)(E.C. 2.7.4.1) from Kingella oralis (ATCC 51147), ppk1 catalyzes the formation of polyphosphate from ATP. false false _1531_ 0 13531 9 Not in stock false We choose to amplify ppk1 gene from Tannerella forsythia (ATCC 43037) since the sequence does not contain any of the illegal restriction sites. false Ma Tsz Shan annotation2343057 1 ppk1 range2343057 1 1 2052 BBa_K1529110 1 BBa_K1529110 ppx 2014-09-11T11:00:00Z 2015-05-08T01:10:50Z 1 1 false false _1911_ 0 22687 9 Not in stock false 1 false Riku Shinohara annotation2383710 1 protein range2383710 1 1 1542 BBa_R0010 1 LacI promoter (lacI regulated) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z The Plac insert was PCR'd from the MG1655 strain of E.coli K12. Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG. false true _1_ 0 24 7 In stock false <P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs. true annotation1961222 1 BBa_R0010 range1961222 1 1 200 annotation1961221 1 end of LacI coding region (inactive) range1961221 1 1 88 annotation1961225 1 -10 range1961225 1 161 166 annotation1961227 1 start range1961227 1 173 173 annotation1961223 1 CAP binding site range1961223 1 89 126 annotation1961224 1 -35 range1961224 1 137 142 annotation1961226 1 LacI binding site range1961226 1 166 200 BBa_K1529111 1 BBa_K1529111 rbs_ppx 2014-09-11T11:00:00Z 2015-05-08T01:10:50Z 1 1 false false _1911_ 0 22687 9 Not in stock false 1 false Riku Shinohara component2382941 1 BBa_B0034 component2382942 1 BBa_K1529110 annotation2382941 1 BBa_B0034 range2382941 1 1 12 annotation2382942 1 BBa_K1529110 range2382942 1 21 69 BBa_K1217002_sequence 1 atgccgcaatctgctcacattctttgccgcgaactgtcgctgttggcgtttaaccgccgcgtgttggcgcaagcgcaagacccgcgcgtgccgctgttggaacgactgcgctttttgtgcatcgtgtcgtccaatctggacgaattttttgaagtgcgcatggcgtatttgcgccgtgaacaacgcgccaaccccacgcaagtgttccaatcgggacaaacgcccagcgaaaccatcacgctggtggcgcaagaagcgcacaagattattgaagagcaatatcaaatatttaaccaagaattgctgcccgcgcttgccgaggaaaatattgtgttttaccgccgcaagcagtggacaagcgcgcagcgcgaatggattgaagattatttcaaccgcgagctgctgccgattctcacgccgattggcttggatgcctcgcatccgtttccgcgtccgctgaataaatcgttgaattttgtggtggaattggatggcaaagatgcgtttggacgcgcttcgggcatggcaatcgtgcaagcgccgcgcattttgccgcgtgcgattcggctgcctgaaaatatttgcgaaggcaaagacggctttgtgtttttgtcgtccattgtgcatgagtatgtgtacaagctgtttcgcggcatgacggtgaaaggctgccatcaatttcgcctaacgcgcgatagcgatttgacggtggaaaacgatgatttgagcaacctgcgcacggcggtgcaaaatgaattgcgcgaccgcgattttggcgacggagtgcggctggaaattgccgaccaatgtcctgattatattagccaatttttgctggaacagtttaagctcacgcgcaatgagttgtatcaagtggaaggtcctgtgaatttggtgcggctgatgtcggtgcccgatatggtgaaccgtcccgatttgaaattcgcgccgttcacgccgcaatatccgcgcgcgttgcgcaaatcggcatcgctgttggacgaaatcgctaagggcgatattttgctgcaccatccgtatcaatcgtttgatccgattgtgaaatttatcaacgatgccacgcgcgatcctgatgtggtggcgattaagatgacgatttaccgcacgggtagccattccgagctggcgcgcgctttgatggcggcggcgctggcgggcaagcaagttaccgtggtggtggaattgatggcgcgttttgacgaagcgaataatgtgtcgtgggcaaccaagctggaagacgcgggcgcgcacgtggtttatggcgtgtttggctacaaggtgcacgccaaaatggcgttggtgattcgccgcgaacaaggcagcctgaaaacctatgcgcatctgggcacgggcaattatcaccaaggcacatcgcgcatctacaccgatttcggcttgctcaccgccaacacggcgataacgcgcgacatcaacacgctatttatggaaatcaccggcttggggcaggcgaacaagctgaaaaaaatctaccaaagcccgttcacgctgcacaaaatgattatggacagcatcgcgcgcgaaaccgaaaacgcgaaaaacggcaagcctgcgcggattatcgccaagctcaattcgctgattgagccgcaagtgatagaggcgttgtatgccgccagcgcggcgggggtgcagattgatttgattgtgcgtggcatgtgcgctttgcgcccacaagtggcggggctgtcggaaaacatccgcgtgcgctccattattgggcggctgctggaacacgcgcgggtttattatttttacaacgatggcgcggaaaacgtgtttatttccagcgcggattggatggggcgcaattttttcagccgcattgaaacctgcacgccgattgaatcgcccgcgctcaaacagcgcgtgatagaagagggcttactgctggcgttgcgcgacaacgtgaaggcttggcagatgcacggcgacggcagctacacgcggattgtggcggacggcgacgaggtgggcattcaagaaacgctgttggcgcgatatacggaatga BBa_R0010_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca BBa_B0034_sequence 1 aaagaggagaaa BBa_K1529111_sequence 1 aaagaggagaaatactagagaatatataatatatatatatatattattaattatatatatatatatata BBa_K1529110_sequence 1 atgccaatacacgataaatcccctcgtccgcaggagtttgctgcggtcgatcttggttcaaacagttttcacatggtcatagcccgtgtggtagatggtgccatgcagattattggccgcctgaaacagcgggtgcatctggcggacggcctggggccagataatatgttgagtgaagaggcaatgacgcgcggtttaaactgtctgtcgctgtttgccgaacggctacaagggttttctcctgccagcgtctgtatagttggtacccatacgctgcgtcaggcgctgaacgccactgactttctgaaacgcgcggaaaaggtcattccctacccgattgaaattatttccggtaatgaagaagcccgtctgatttttatgggcgtggaacatacccaaccggaaaaaggtcgcaaactggttattgatattggcggcggatctacggaactggtgattggtgaaaatttcgaacctattctcgttgaaagccgccggatgggttgtgtcagctttgcccagctttattttcctggcggggtcatcaataaagagaattttcagcgcgctcgcatggcggcagcacaaaaactggaaactttaacctggcaattccgtattcagggctggaacgttgcaatgggcgcttccggtaccataaaagccgcccatgaagtgttaatggaaatgggcgagaaagacgggataattaccccggaacgtctggaaaaactggtaaaagaagttttacgtcaccgtaatttcgcatcgctgagtttaccgggtctttccgaagagcggaaaacagtcttcgttccgggactggcgattttatgcggtgtgtttgatgctttagccatccgtgaactgcgcctttctgacggggcgttacgcgaaggcgtactgtatgaaatggaaggacgtttccgtcatcaggatgtgcgtagtcgcaccgccagcagcctcgccaaccagtatcacatcgacagcgaacaggcccgacgggtgctggataccactatgcaaatgtacgaacagtggcgggaacagcaaccgaagctggcgcatccgcaactggaggcgctactgcgatgggccgccatgctgcatgaggtcgggttgaatatcaaccacagcggtttgcatcgccactccgcttatattctgcaaaacagtgacttgccgggttttaatcaggaacagcagctgatgatggcgacactggtgcgctatcaccgtaaagcgattaagctcgacgatctaccgcgctttaccttgtttaagaagaaacagttcctgccactgatacagctattgcgccttggcgtattactcaacaatcaacgtcaggcaaccaccacaccgccaacattgacgctgattactgatgacagtcactggacactgcgtttcccgcatgactggtttagtcagaatgcgctggtactgcttgatctggaaaaggagcaagaatactgggaaggcgtggctggctggcggttgaaaattgaagaagaaagtacaccagaaatcgccgcttaa BBa_K1529103_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagagaatatataatatatatatatatattattaattatatatatatatatatatactagagaaagaggagaaatactagatgccgcaatctgctcacattctttgccgcgaactgtcgctgttggcgtttaaccgccgcgtgttggcgcaagcgcaagacccgcgcgtgccgctgttggaacgactgcgctttttgtgcatcgtgtcgtccaatctggacgaattttttgaagtgcgcatggcgtatttgcgccgtgaacaacgcgccaaccccacgcaagtgttccaatcgggacaaacgcccagcgaaaccatcacgctggtggcgcaagaagcgcacaagattattgaagagcaatatcaaatatttaaccaagaattgctgcccgcgcttgccgaggaaaatattgtgttttaccgccgcaagcagtggacaagcgcgcagcgcgaatggattgaagattatttcaaccgcgagctgctgccgattctcacgccgattggcttggatgcctcgcatccgtttccgcgtccgctgaataaatcgttgaattttgtggtggaattggatggcaaagatgcgtttggacgcgcttcgggcatggcaatcgtgcaagcgccgcgcattttgccgcgtgcgattcggctgcctgaaaatatttgcgaaggcaaagacggctttgtgtttttgtcgtccattgtgcatgagtatgtgtacaagctgtttcgcggcatgacggtgaaaggctgccatcaatttcgcctaacgcgcgatagcgatttgacggtggaaaacgatgatttgagcaacctgcgcacggcggtgcaaaatgaattgcgcgaccgcgattttggcgacggagtgcggctggaaattgccgaccaatgtcctgattatattagccaatttttgctggaacagtttaagctcacgcgcaatgagttgtatcaagtggaaggtcctgtgaatttggtgcggctgatgtcggtgcccgatatggtgaaccgtcccgatttgaaattcgcgccgttcacgccgcaatatccgcgcgcgttgcgcaaatcggcatcgctgttggacgaaatcgctaagggcgatattttgctgcaccatccgtatcaatcgtttgatccgattgtgaaatttatcaacgatgccacgcgcgatcctgatgtggtggcgattaagatgacgatttaccgcacgggtagccattccgagctggcgcgcgctttgatggcggcggcgctggcgggcaagcaagttaccgtggtggtggaattgatggcgcgttttgacgaagcgaataatgtgtcgtgggcaaccaagctggaagacgcgggcgcgcacgtggtttatggcgtgtttggctacaaggtgcacgccaaaatggcgttggtgattcgccgcgaacaaggcagcctgaaaacctatgcgcatctgggcacgggcaattatcaccaaggcacatcgcgcatctacaccgatttcggcttgctcaccgccaacacggcgataacgcgcgacatcaacacgctatttatggaaatcaccggcttggggcaggcgaacaagctgaaaaaaatctaccaaagcccgttcacgctgcacaaaatgattatggacagcatcgcgcgcgaaaccgaaaacgcgaaaaacggcaagcctgcgcggattatcgccaagctcaattcgctgattgagccgcaagtgatagaggcgttgtatgccgccagcgcggcgggggtgcagattgatttgattgtgcgtggcatgtgcgctttgcgcccacaagtggcggggctgtcggaaaacatccgcgtgcgctccattattgggcggctgctggaacacgcgcgggtttattatttttacaacgatggcgcggaaaacgtgtttatttccagcgcggattggatggggcgcaattttttcagccgcattgaaacctgcacgccgattgaatcgcccgcgctcaaacagcgcgtgatagaagagggcttactgctggcgttgcgcgacaacgtgaaggcttggcagatgcacggcgacggcagctacacgcggattgtggcggacggcgacgaggtgggcattcaagaaacgctgttggcgcgatatacggaatga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z