LOCUS       BBa_K1529104            3356 bp    DNA     linear       27-Dec-2024
DEFINITION  PcIλ_rbs_ackA_rbs_ppk1
ACCESSION   BBa_K1529104
VERSION     BBa_K1529104.1  
FEATURES             Location/Qualifiers
     promoter        38..43
                     /label=-10
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        1..49
                     /label=BBa_R0051
     misc_feature    62..65
                     /label=conserved
     RBS             58..69
                     /label=BBa_B0034
     CDS             76..1278
                     /label=BBa_K737002
     misc_feature    1291..1294
                     /label=conserved
     RBS             1287..1298
                     /label=BBa_B0034
     CDS             1305..3356
                     /label=ppk1
     CDS             1305..3356
                     /label=BBa_K1217002
     misc_feature    1287..3356
                     /label=BBa_K1529100
ORIGIN
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagaaa
       61 gaggagaaat actagatgtc gagtaagtta gtactggttc tgaactgcgg tagttcttca
      121 ctgaaatttg ccatcatcga tgcagtaaat ggtgaagagt acctttctgg tttagccgaa
      181 tgtttccacc tgcccgaagc acgtatcaaa tggaaaatgg acggcaataa acaggaagcg
      241 gctttaggtg caggcgccgc tcacagcgaa gcgctcaact ttatcgttaa tactattctg
      301 gcacaaaaac cagaactgtc tgcgcagctg actgctatcg gtcaccgtat cgtacacggc
      361 ggcgaaaagt ataccagctc cgtagtgatc gatgagtctg ttattcaggg tatcaaagat
      421 gcagcttctt ttgcaccgct gcacaacccg gctcacctga tcggtatcga agaagctctg
      481 aaatctttcc cacagctgaa agacaaaaac gttgctgtat ttgacaccgc gttccaccag
      541 actatgccgg aagagtctta cctctacgcc ctgccttaca acctgtacaa agagcacggc
      601 atccgtcgtt acggcgcgca cggcaccagc cacttctatg taacccagga agcggcaaaa
      661 atgctgaaca aaccggtaga agaactgaac atcatcacct gccacctggg caacggtggt
      721 tccgtttctg ctatccgcaa cggtaaatgc gttgacacct ctatgggcct gaccccgctg
      781 gaaggtctgg tcatgggtac ccgttctggt gatatcgatc cggcgatcat cttccacctg
      841 cacgacaccc tgggcatgag cgttgacgca atcaacaaac tgctgaccaa agagtctggc
      901 ctgctgggtc tgaccgaagt gaccagcgac tgccgctatg ttgaagacaa ctacgcgacg
      961 aaagaagacg cgaagcgcgc aatggacgtt tactgccacc gcctggcgaa atacatcggt
     1021 gcctacactg cgctgatgga tggtcgtctg gacgctgttg tattcactgg tggtatcggt
     1081 gaaaatgccg caatggttcg tgaactgtct ctgggcaaac tgggcgtgct gggctttgaa
     1141 gttgatcatg aacgcaacct ggctgcacgt ttcggcaaat ctggtttcat caacaaagaa
     1201 ggtacccgtc ctgcggtggt tatcccaacc aacgaagaac tggttatcgc gcaagacgcg
     1261 agccgcctga ctgcctgata ctagagaaag aggagaaata ctagatgccg caatctgctc
     1321 acattctttg ccgcgaactg tcgctgttgg cgtttaaccg ccgcgtgttg gcgcaagcgc
     1381 aagacccgcg cgtgccgctg ttggaacgac tgcgcttttt gtgcatcgtg tcgtccaatc
     1441 tggacgaatt ttttgaagtg cgcatggcgt atttgcgccg tgaacaacgc gccaacccca
     1501 cgcaagtgtt ccaatcggga caaacgccca gcgaaaccat cacgctggtg gcgcaagaag
     1561 cgcacaagat tattgaagag caatatcaaa tatttaacca agaattgctg cccgcgcttg
     1621 ccgaggaaaa tattgtgttt taccgccgca agcagtggac aagcgcgcag cgcgaatgga
     1681 ttgaagatta tttcaaccgc gagctgctgc cgattctcac gccgattggc ttggatgcct
     1741 cgcatccgtt tccgcgtccg ctgaataaat cgttgaattt tgtggtggaa ttggatggca
     1801 aagatgcgtt tggacgcgct tcgggcatgg caatcgtgca agcgccgcgc attttgccgc
     1861 gtgcgattcg gctgcctgaa aatatttgcg aaggcaaaga cggctttgtg tttttgtcgt
     1921 ccattgtgca tgagtatgtg tacaagctgt ttcgcggcat gacggtgaaa ggctgccatc
     1981 aatttcgcct aacgcgcgat agcgatttga cggtggaaaa cgatgatttg agcaacctgc
     2041 gcacggcggt gcaaaatgaa ttgcgcgacc gcgattttgg cgacggagtg cggctggaaa
     2101 ttgccgacca atgtcctgat tatattagcc aatttttgct ggaacagttt aagctcacgc
     2161 gcaatgagtt gtatcaagtg gaaggtcctg tgaatttggt gcggctgatg tcggtgcccg
     2221 atatggtgaa ccgtcccgat ttgaaattcg cgccgttcac gccgcaatat ccgcgcgcgt
     2281 tgcgcaaatc ggcatcgctg ttggacgaaa tcgctaaggg cgatattttg ctgcaccatc
     2341 cgtatcaatc gtttgatccg attgtgaaat ttatcaacga tgccacgcgc gatcctgatg
     2401 tggtggcgat taagatgacg atttaccgca cgggtagcca ttccgagctg gcgcgcgctt
     2461 tgatggcggc ggcgctggcg ggcaagcaag ttaccgtggt ggtggaattg atggcgcgtt
     2521 ttgacgaagc gaataatgtg tcgtgggcaa ccaagctgga agacgcgggc gcgcacgtgg
     2581 tttatggcgt gtttggctac aaggtgcacg ccaaaatggc gttggtgatt cgccgcgaac
     2641 aaggcagcct gaaaacctat gcgcatctgg gcacgggcaa ttatcaccaa ggcacatcgc
     2701 gcatctacac cgatttcggc ttgctcaccg ccaacacggc gataacgcgc gacatcaaca
     2761 cgctatttat ggaaatcacc ggcttggggc aggcgaacaa gctgaaaaaa atctaccaaa
     2821 gcccgttcac gctgcacaaa atgattatgg acagcatcgc gcgcgaaacc gaaaacgcga
     2881 aaaacggcaa gcctgcgcgg attatcgcca agctcaattc gctgattgag ccgcaagtga
     2941 tagaggcgtt gtatgccgcc agcgcggcgg gggtgcagat tgatttgatt gtgcgtggca
     3001 tgtgcgcttt gcgcccacaa gtggcggggc tgtcggaaaa catccgcgtg cgctccatta
     3061 ttgggcggct gctggaacac gcgcgggttt attattttta caacgatggc gcggaaaacg
     3121 tgtttatttc cagcgcggat tggatggggc gcaatttttt cagccgcatt gaaacctgca
     3181 cgccgattga atcgcccgcg ctcaaacagc gcgtgataga agagggctta ctgctggcgt
     3241 tgcgcgacaa cgtgaaggct tggcagatgc acggcgacgg cagctacacg cggattgtgg
     3301 cggacggcga cgaggtgggc attcaagaaa cgctgttggc gcgatatacg gaatga
//