BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1217002 1 BBa_K1217002 ppk1(K.oralis) 2013-09-14T11:00:00Z 2015-05-08T01:09:42Z It is from Kingella oralis (ATCC 51147) genomic DNA Coding sequence of polyphosphate kinase (ppk1)(E.C. 2.7.4.1) from Kingella oralis (ATCC 51147), ppk1 catalyzes the formation of polyphosphate from ATP. false false _1531_ 0 13531 9 Not in stock false We choose to amplify ppk1 gene from Tannerella forsythia (ATCC 43037) since the sequence does not contain any of the illegal restriction sites. false Ma Tsz Shan annotation2343057 1 ppk1 range2343057 1 1 2052 BBa_K525998 1 pT7 Promoter T7 and RBS 2011-09-12T11:00:00Z 2015-05-08T01:12:35Z Synthesis Released HQ 2013 Promoter T7 and RBS false false _690_ 0 8543 9 In stock false Synthesis false Anna Drong annotation2129432 1 T7 promoter range2129432 1 1 20 annotation2129433 1 RBS range2129433 1 24 27 annotation2127788 1 B0034 range2127788 1 21 32 BBa_K1529111 1 BBa_K1529111 rbs_ppx 2014-09-11T11:00:00Z 2015-05-08T01:10:50Z 1 1 false false _1911_ 0 22687 9 Not in stock false 1 false Riku Shinohara component2382942 1 BBa_K1529110 component2382941 1 BBa_B0034 annotation2382941 1 BBa_B0034 range2382941 1 1 12 annotation2382942 1 BBa_K1529110 range2382942 1 21 69 BBa_K1529110 1 BBa_K1529110 ppx 2014-09-11T11:00:00Z 2015-05-08T01:10:50Z 1 1 false false _1911_ 0 22687 9 Not in stock false 1 false Riku Shinohara annotation2383710 1 protein range2383710 1 1 1542 BBa_K1529105 1 BBa_K1529105 Pt7_rbs_ppk1_rbs_ppx 2014-09-14T11:00:00Z 2015-05-08T01:10:50Z 1 1 false false _1911_ 0 22685 9 Not in stock false 1 false Shoko Suzuki component2383640 1 BBa_K1529111 component2383636 1 BBa_K1217003 annotation2383636 1 BBa_K1217003 range2383636 1 1 2090 annotation2383640 1 BBa_K1529111 range2383640 1 2099 2167 BBa_K1217003 1 BBa_K1217003 pT7-ppk1(K.oralis) 2013-09-14T11:00:00Z 2015-05-08T01:09:42Z The coding gene ppk1(E.C. 2.7.4.1) is amplified from Kingella oralis (ATCC 51147) genomic DNA. Polyphosphate kinase (ppk1) (E.C. 2.7.4.1) catalyzes the formation of polyphosphate from ATP. In BBa_K1217003, ppk1 was under the control of T7 promotor. Under iptg induction, over-expression of ppk1 can reduce the phosphate level in the medium by its incorporation into polyphosphate synthesis inside the bacteria. false false _1531_ 0 13531 9 In stock true We choose to amplify ppk1 gene from Kingella oralis (ATCC 51147) since the sequence does not contain any of the illegal restriction sites. false Ma Tsz Shan component2343092 1 BBa_K1217002 component2343090 1 BBa_K525998 annotation2343090 1 BBa_K525998 range2343090 1 1 32 annotation2343092 1 BBa_K1217002 range2343092 1 39 2090 BBa_K1217002_sequence 1 atgccgcaatctgctcacattctttgccgcgaactgtcgctgttggcgtttaaccgccgcgtgttggcgcaagcgcaagacccgcgcgtgccgctgttggaacgactgcgctttttgtgcatcgtgtcgtccaatctggacgaattttttgaagtgcgcatggcgtatttgcgccgtgaacaacgcgccaaccccacgcaagtgttccaatcgggacaaacgcccagcgaaaccatcacgctggtggcgcaagaagcgcacaagattattgaagagcaatatcaaatatttaaccaagaattgctgcccgcgcttgccgaggaaaatattgtgttttaccgccgcaagcagtggacaagcgcgcagcgcgaatggattgaagattatttcaaccgcgagctgctgccgattctcacgccgattggcttggatgcctcgcatccgtttccgcgtccgctgaataaatcgttgaattttgtggtggaattggatggcaaagatgcgtttggacgcgcttcgggcatggcaatcgtgcaagcgccgcgcattttgccgcgtgcgattcggctgcctgaaaatatttgcgaaggcaaagacggctttgtgtttttgtcgtccattgtgcatgagtatgtgtacaagctgtttcgcggcatgacggtgaaaggctgccatcaatttcgcctaacgcgcgatagcgatttgacggtggaaaacgatgatttgagcaacctgcgcacggcggtgcaaaatgaattgcgcgaccgcgattttggcgacggagtgcggctggaaattgccgaccaatgtcctgattatattagccaatttttgctggaacagtttaagctcacgcgcaatgagttgtatcaagtggaaggtcctgtgaatttggtgcggctgatgtcggtgcccgatatggtgaaccgtcccgatttgaaattcgcgccgttcacgccgcaatatccgcgcgcgttgcgcaaatcggcatcgctgttggacgaaatcgctaagggcgatattttgctgcaccatccgtatcaatcgtttgatccgattgtgaaatttatcaacgatgccacgcgcgatcctgatgtggtggcgattaagatgacgatttaccgcacgggtagccattccgagctggcgcgcgctttgatggcggcggcgctggcgggcaagcaagttaccgtggtggtggaattgatggcgcgttttgacgaagcgaataatgtgtcgtgggcaaccaagctggaagacgcgggcgcgcacgtggtttatggcgtgtttggctacaaggtgcacgccaaaatggcgttggtgattcgccgcgaacaaggcagcctgaaaacctatgcgcatctgggcacgggcaattatcaccaaggcacatcgcgcatctacaccgatttcggcttgctcaccgccaacacggcgataacgcgcgacatcaacacgctatttatggaaatcaccggcttggggcaggcgaacaagctgaaaaaaatctaccaaagcccgttcacgctgcacaaaatgattatggacagcatcgcgcgcgaaaccgaaaacgcgaaaaacggcaagcctgcgcggattatcgccaagctcaattcgctgattgagccgcaagtgatagaggcgttgtatgccgccagcgcggcgggggtgcagattgatttgattgtgcgtggcatgtgcgctttgcgcccacaagtggcggggctgtcggaaaacatccgcgtgcgctccattattgggcggctgctggaacacgcgcgggtttattatttttacaacgatggcgcggaaaacgtgtttatttccagcgcggattggatggggcgcaattttttcagccgcattgaaacctgcacgccgattgaatcgcccgcgctcaaacagcgcgtgatagaagagggcttactgctggcgttgcgcgacaacgtgaaggcttggcagatgcacggcgacggcagctacacgcggattgtggcggacggcgacgaggtgggcattcaagaaacgctgttggcgcgatatacggaatga BBa_B0034_sequence 1 aaagaggagaaa BBa_K525998_sequence 1 taatacgactcactatagggaaagaggagaaa BBa_K1217003_sequence 1 taatacgactcactatagggaaagaggagaaatactagatgccgcaatctgctcacattctttgccgcgaactgtcgctgttggcgtttaaccgccgcgtgttggcgcaagcgcaagacccgcgcgtgccgctgttggaacgactgcgctttttgtgcatcgtgtcgtccaatctggacgaattttttgaagtgcgcatggcgtatttgcgccgtgaacaacgcgccaaccccacgcaagtgttccaatcgggacaaacgcccagcgaaaccatcacgctggtggcgcaagaagcgcacaagattattgaagagcaatatcaaatatttaaccaagaattgctgcccgcgcttgccgaggaaaatattgtgttttaccgccgcaagcagtggacaagcgcgcagcgcgaatggattgaagattatttcaaccgcgagctgctgccgattctcacgccgattggcttggatgcctcgcatccgtttccgcgtccgctgaataaatcgttgaattttgtggtggaattggatggcaaagatgcgtttggacgcgcttcgggcatggcaatcgtgcaagcgccgcgcattttgccgcgtgcgattcggctgcctgaaaatatttgcgaaggcaaagacggctttgtgtttttgtcgtccattgtgcatgagtatgtgtacaagctgtttcgcggcatgacggtgaaaggctgccatcaatttcgcctaacgcgcgatagcgatttgacggtggaaaacgatgatttgagcaacctgcgcacggcggtgcaaaatgaattgcgcgaccgcgattttggcgacggagtgcggctggaaattgccgaccaatgtcctgattatattagccaatttttgctggaacagtttaagctcacgcgcaatgagttgtatcaagtggaaggtcctgtgaatttggtgcggctgatgtcggtgcccgatatggtgaaccgtcccgatttgaaattcgcgccgttcacgccgcaatatccgcgcgcgttgcgcaaatcggcatcgctgttggacgaaatcgctaagggcgatattttgctgcaccatccgtatcaatcgtttgatccgattgtgaaatttatcaacgatgccacgcgcgatcctgatgtggtggcgattaagatgacgatttaccgcacgggtagccattccgagctggcgcgcgctttgatggcggcggcgctggcgggcaagcaagttaccgtggtggtggaattgatggcgcgttttgacgaagcgaataatgtgtcgtgggcaaccaagctggaagacgcgggcgcgcacgtggtttatggcgtgtttggctacaaggtgcacgccaaaatggcgttggtgattcgccgcgaacaaggcagcctgaaaacctatgcgcatctgggcacgggcaattatcaccaaggcacatcgcgcatctacaccgatttcggcttgctcaccgccaacacggcgataacgcgcgacatcaacacgctatttatggaaatcaccggcttggggcaggcgaacaagctgaaaaaaatctaccaaagcccgttcacgctgcacaaaatgattatggacagcatcgcgcgcgaaaccgaaaacgcgaaaaacggcaagcctgcgcggattatcgccaagctcaattcgctgattgagccgcaagtgatagaggcgttgtatgccgccagcgcggcgggggtgcagattgatttgattgtgcgtggcatgtgcgctttgcgcccacaagtggcggggctgtcggaaaacatccgcgtgcgctccattattgggcggctgctggaacacgcgcgggtttattatttttacaacgatggcgcggaaaacgtgtttatttccagcgcggattggatggggcgcaattttttcagccgcattgaaacctgcacgccgattgaatcgcccgcgctcaaacagcgcgtgatagaagagggcttactgctggcgttgcgcgacaacgtgaaggcttggcagatgcacggcgacggcagctacacgcggattgtggcggacggcgacgaggtgggcattcaagaaacgctgttggcgcgatatacggaatga BBa_K1529111_sequence 1 aaagaggagaaatactagagaatatataatatatatatatatattattaattatatatatatatatata BBa_K1529110_sequence 1 atgccaatacacgataaatcccctcgtccgcaggagtttgctgcggtcgatcttggttcaaacagttttcacatggtcatagcccgtgtggtagatggtgccatgcagattattggccgcctgaaacagcgggtgcatctggcggacggcctggggccagataatatgttgagtgaagaggcaatgacgcgcggtttaaactgtctgtcgctgtttgccgaacggctacaagggttttctcctgccagcgtctgtatagttggtacccatacgctgcgtcaggcgctgaacgccactgactttctgaaacgcgcggaaaaggtcattccctacccgattgaaattatttccggtaatgaagaagcccgtctgatttttatgggcgtggaacatacccaaccggaaaaaggtcgcaaactggttattgatattggcggcggatctacggaactggtgattggtgaaaatttcgaacctattctcgttgaaagccgccggatgggttgtgtcagctttgcccagctttattttcctggcggggtcatcaataaagagaattttcagcgcgctcgcatggcggcagcacaaaaactggaaactttaacctggcaattccgtattcagggctggaacgttgcaatgggcgcttccggtaccataaaagccgcccatgaagtgttaatggaaatgggcgagaaagacgggataattaccccggaacgtctggaaaaactggtaaaagaagttttacgtcaccgtaatttcgcatcgctgagtttaccgggtctttccgaagagcggaaaacagtcttcgttccgggactggcgattttatgcggtgtgtttgatgctttagccatccgtgaactgcgcctttctgacggggcgttacgcgaaggcgtactgtatgaaatggaaggacgtttccgtcatcaggatgtgcgtagtcgcaccgccagcagcctcgccaaccagtatcacatcgacagcgaacaggcccgacgggtgctggataccactatgcaaatgtacgaacagtggcgggaacagcaaccgaagctggcgcatccgcaactggaggcgctactgcgatgggccgccatgctgcatgaggtcgggttgaatatcaaccacagcggtttgcatcgccactccgcttatattctgcaaaacagtgacttgccgggttttaatcaggaacagcagctgatgatggcgacactggtgcgctatcaccgtaaagcgattaagctcgacgatctaccgcgctttaccttgtttaagaagaaacagttcctgccactgatacagctattgcgccttggcgtattactcaacaatcaacgtcaggcaaccaccacaccgccaacattgacgctgattactgatgacagtcactggacactgcgtttcccgcatgactggtttagtcagaatgcgctggtactgcttgatctggaaaaggagcaagaatactgggaaggcgtggctggctggcggttgaaaattgaagaagaaagtacaccagaaatcgccgcttaa BBa_K1529105_sequence 1 taatacgactcactatagggaaagaggagaaatactagatgccgcaatctgctcacattctttgccgcgaactgtcgctgttggcgtttaaccgccgcgtgttggcgcaagcgcaagacccgcgcgtgccgctgttggaacgactgcgctttttgtgcatcgtgtcgtccaatctggacgaattttttgaagtgcgcatggcgtatttgcgccgtgaacaacgcgccaaccccacgcaagtgttccaatcgggacaaacgcccagcgaaaccatcacgctggtggcgcaagaagcgcacaagattattgaagagcaatatcaaatatttaaccaagaattgctgcccgcgcttgccgaggaaaatattgtgttttaccgccgcaagcagtggacaagcgcgcagcgcgaatggattgaagattatttcaaccgcgagctgctgccgattctcacgccgattggcttggatgcctcgcatccgtttccgcgtccgctgaataaatcgttgaattttgtggtggaattggatggcaaagatgcgtttggacgcgcttcgggcatggcaatcgtgcaagcgccgcgcattttgccgcgtgcgattcggctgcctgaaaatatttgcgaaggcaaagacggctttgtgtttttgtcgtccattgtgcatgagtatgtgtacaagctgtttcgcggcatgacggtgaaaggctgccatcaatttcgcctaacgcgcgatagcgatttgacggtggaaaacgatgatttgagcaacctgcgcacggcggtgcaaaatgaattgcgcgaccgcgattttggcgacggagtgcggctggaaattgccgaccaatgtcctgattatattagccaatttttgctggaacagtttaagctcacgcgcaatgagttgtatcaagtggaaggtcctgtgaatttggtgcggctgatgtcggtgcccgatatggtgaaccgtcccgatttgaaattcgcgccgttcacgccgcaatatccgcgcgcgttgcgcaaatcggcatcgctgttggacgaaatcgctaagggcgatattttgctgcaccatccgtatcaatcgtttgatccgattgtgaaatttatcaacgatgccacgcgcgatcctgatgtggtggcgattaagatgacgatttaccgcacgggtagccattccgagctggcgcgcgctttgatggcggcggcgctggcgggcaagcaagttaccgtggtggtggaattgatggcgcgttttgacgaagcgaataatgtgtcgtgggcaaccaagctggaagacgcgggcgcgcacgtggtttatggcgtgtttggctacaaggtgcacgccaaaatggcgttggtgattcgccgcgaacaaggcagcctgaaaacctatgcgcatctgggcacgggcaattatcaccaaggcacatcgcgcatctacaccgatttcggcttgctcaccgccaacacggcgataacgcgcgacatcaacacgctatttatggaaatcaccggcttggggcaggcgaacaagctgaaaaaaatctaccaaagcccgttcacgctgcacaaaatgattatggacagcatcgcgcgcgaaaccgaaaacgcgaaaaacggcaagcctgcgcggattatcgccaagctcaattcgctgattgagccgcaagtgatagaggcgttgtatgccgccagcgcggcgggggtgcagattgatttgattgtgcgtggcatgtgcgctttgcgcccacaagtggcggggctgtcggaaaacatccgcgtgcgctccattattgggcggctgctggaacacgcgcgggtttattatttttacaacgatggcgcggaaaacgtgtttatttccagcgcggattggatggggcgcaattttttcagccgcattgaaacctgcacgccgattgaatcgcccgcgctcaaacagcgcgtgatagaagagggcttactgctggcgttgcgcgacaacgtgaaggcttggcagatgcacggcgacggcagctacacgcggattgtggcggacggcgacgaggtgggcattcaagaaacgctgttggcgcgatatacggaatgatactagagaaagaggagaaatactagagaatatataatatatatatatatattattaattatatatatatatatata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z