LOCUS       BBa_K1529106            3319 bp    DNA     linear       13-May-2024
DEFINITION  Pt7_rbs_ppk1_rbs_AckA
ACCESSION   BBa_K1529106
VERSION     BBa_K1529106.1  
FEATURES             Location/Qualifiers
     misc_feature    21..32
                     /label=B0034
     promoter        1..20
                     /label=T7 promoter
     RBS             24..27
                     /label=RBS
     promoter        1..32
                     /label=BBa_K525998
     CDS             39..2090
                     /label=ppk1
     CDS             39..2090
                     /label=BBa_K1217002
     misc_feature    1..2090
                     /label=BBa_K1217003
     misc_feature    2103..2106
                     /label=conserved
     RBS             2099..2110
                     /label=BBa_B0034
     CDS             2117..3319
                     /label=BBa_K737002
     misc_feature    2099..3319
                     /label=BBa_K1529130
ORIGIN
        1 taatacgact cactataggg aaagaggaga aatactagat gccgcaatct gctcacattc
       61 tttgccgcga actgtcgctg ttggcgttta accgccgcgt gttggcgcaa gcgcaagacc
      121 cgcgcgtgcc gctgttggaa cgactgcgct ttttgtgcat cgtgtcgtcc aatctggacg
      181 aattttttga agtgcgcatg gcgtatttgc gccgtgaaca acgcgccaac cccacgcaag
      241 tgttccaatc gggacaaacg cccagcgaaa ccatcacgct ggtggcgcaa gaagcgcaca
      301 agattattga agagcaatat caaatattta accaagaatt gctgcccgcg cttgccgagg
      361 aaaatattgt gttttaccgc cgcaagcagt ggacaagcgc gcagcgcgaa tggattgaag
      421 attatttcaa ccgcgagctg ctgccgattc tcacgccgat tggcttggat gcctcgcatc
      481 cgtttccgcg tccgctgaat aaatcgttga attttgtggt ggaattggat ggcaaagatg
      541 cgtttggacg cgcttcgggc atggcaatcg tgcaagcgcc gcgcattttg ccgcgtgcga
      601 ttcggctgcc tgaaaatatt tgcgaaggca aagacggctt tgtgtttttg tcgtccattg
      661 tgcatgagta tgtgtacaag ctgtttcgcg gcatgacggt gaaaggctgc catcaatttc
      721 gcctaacgcg cgatagcgat ttgacggtgg aaaacgatga tttgagcaac ctgcgcacgg
      781 cggtgcaaaa tgaattgcgc gaccgcgatt ttggcgacgg agtgcggctg gaaattgccg
      841 accaatgtcc tgattatatt agccaatttt tgctggaaca gtttaagctc acgcgcaatg
      901 agttgtatca agtggaaggt cctgtgaatt tggtgcggct gatgtcggtg cccgatatgg
      961 tgaaccgtcc cgatttgaaa ttcgcgccgt tcacgccgca atatccgcgc gcgttgcgca
     1021 aatcggcatc gctgttggac gaaatcgcta agggcgatat tttgctgcac catccgtatc
     1081 aatcgtttga tccgattgtg aaatttatca acgatgccac gcgcgatcct gatgtggtgg
     1141 cgattaagat gacgatttac cgcacgggta gccattccga gctggcgcgc gctttgatgg
     1201 cggcggcgct ggcgggcaag caagttaccg tggtggtgga attgatggcg cgttttgacg
     1261 aagcgaataa tgtgtcgtgg gcaaccaagc tggaagacgc gggcgcgcac gtggtttatg
     1321 gcgtgtttgg ctacaaggtg cacgccaaaa tggcgttggt gattcgccgc gaacaaggca
     1381 gcctgaaaac ctatgcgcat ctgggcacgg gcaattatca ccaaggcaca tcgcgcatct
     1441 acaccgattt cggcttgctc accgccaaca cggcgataac gcgcgacatc aacacgctat
     1501 ttatggaaat caccggcttg gggcaggcga acaagctgaa aaaaatctac caaagcccgt
     1561 tcacgctgca caaaatgatt atggacagca tcgcgcgcga aaccgaaaac gcgaaaaacg
     1621 gcaagcctgc gcggattatc gccaagctca attcgctgat tgagccgcaa gtgatagagg
     1681 cgttgtatgc cgccagcgcg gcgggggtgc agattgattt gattgtgcgt ggcatgtgcg
     1741 ctttgcgccc acaagtggcg gggctgtcgg aaaacatccg cgtgcgctcc attattgggc
     1801 ggctgctgga acacgcgcgg gtttattatt tttacaacga tggcgcggaa aacgtgttta
     1861 tttccagcgc ggattggatg gggcgcaatt ttttcagccg cattgaaacc tgcacgccga
     1921 ttgaatcgcc cgcgctcaaa cagcgcgtga tagaagaggg cttactgctg gcgttgcgcg
     1981 acaacgtgaa ggcttggcag atgcacggcg acggcagcta cacgcggatt gtggcggacg
     2041 gcgacgaggt gggcattcaa gaaacgctgt tggcgcgata tacggaatga tactagagaa
     2101 agaggagaaa tactagatgt cgagtaagtt agtactggtt ctgaactgcg gtagttcttc
     2161 actgaaattt gccatcatcg atgcagtaaa tggtgaagag tacctttctg gtttagccga
     2221 atgtttccac ctgcccgaag cacgtatcaa atggaaaatg gacggcaata aacaggaagc
     2281 ggctttaggt gcaggcgccg ctcacagcga agcgctcaac tttatcgtta atactattct
     2341 ggcacaaaaa ccagaactgt ctgcgcagct gactgctatc ggtcaccgta tcgtacacgg
     2401 cggcgaaaag tataccagct ccgtagtgat cgatgagtct gttattcagg gtatcaaaga
     2461 tgcagcttct tttgcaccgc tgcacaaccc ggctcacctg atcggtatcg aagaagctct
     2521 gaaatctttc ccacagctga aagacaaaaa cgttgctgta tttgacaccg cgttccacca
     2581 gactatgccg gaagagtctt acctctacgc cctgccttac aacctgtaca aagagcacgg
     2641 catccgtcgt tacggcgcgc acggcaccag ccacttctat gtaacccagg aagcggcaaa
     2701 aatgctgaac aaaccggtag aagaactgaa catcatcacc tgccacctgg gcaacggtgg
     2761 ttccgtttct gctatccgca acggtaaatg cgttgacacc tctatgggcc tgaccccgct
     2821 ggaaggtctg gtcatgggta cccgttctgg tgatatcgat ccggcgatca tcttccacct
     2881 gcacgacacc ctgggcatga gcgttgacgc aatcaacaaa ctgctgacca aagagtctgg
     2941 cctgctgggt ctgaccgaag tgaccagcga ctgccgctat gttgaagaca actacgcgac
     3001 gaaagaagac gcgaagcgcg caatggacgt ttactgccac cgcctggcga aatacatcgg
     3061 tgcctacact gcgctgatgg atggtcgtct ggacgctgtt gtattcactg gtggtatcgg
     3121 tgaaaatgcc gcaatggttc gtgaactgtc tctgggcaaa ctgggcgtgc tgggctttga
     3181 agttgatcat gaacgcaacc tggctgcacg tttcggcaaa tctggtttca tcaacaaaga
     3241 aggtacccgt cctgcggtgg ttatcccaac caacgaagaa ctggttatcg cgcaagacgc
     3301 gagccgcctg actgcctga
//