LOCUS       BBa_K1529200            1601 bp    DNA     linear       27-Dec-2024
DEFINITION  Ptet_tolC
ACCESSION   BBa_K1529200
VERSION     BBa_K1529200.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..77
                     /label=RBS
     CDS             78..1595
                     /label=TolC
     misc_feature    1596..1601
                     /label=stop
     CDS             63..1601
                     /label=BBa_K554009
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agattaaaga ggagaaaatg attgccctga acactgcctc gccacagggg atgcagatga
      121 agaagctcct gccgatcctg atcgggctaa gtctttccgg gttcagctca ctttcacaag
      181 ccgagaatct tatgcaagta tatcagcagg cacggctgtc gaatccagag cttcgtaaat
      241 cagcggccga tcgagatgct gcgttcgaga aaattaatga ggcccggtca ccgctgttac
      301 cacagctggg tctcggcgcg gattatacat acagcaacgg ctaccgtgat gcgaatggaa
      361 taaatagtaa cgcaaccagc gcaagcttac aactgacaca gtcgatcttc gatatgtcga
      421 aatggcgcgc actaacgctt caggaaaaag cagccgggat ccaagatgta acttatcaga
      481 ccgaccagca aacattgatt ttgaatactg caaccgctta ctttaacgtt ctgaatgcca
      541 tcgacgtgct gagctacacc caggcacaaa aggaagccat ttatcgtcaa cttgaccaga
      601 ctacgcagcg gtttaacgtt gggttagtag caatcaccga tgttcaaaat gcgcgtgcgc
      661 agtatgatac cgttctggcc aatgaggtca ccgcacgcaa caatcttgat aatgcggttg
      721 aacagttacg tcagattacc gggaactatt atcccgagct ggcagcgctg aatgtggaaa
      781 attttaaaac tgacaaacct caacctgtca ataccctgct caaggaagcc gagaaacgca
      841 acctgtctct attgcaagct cgcctgtctc aagacctggc tcgggagcaa atacgccagg
      901 cacaggacgg acatctgccc acactggacc tgaccgcaag tactgggata tccgacacca
      961 gttactctgg atcaaaaact cgtggcgccg ccggaacaca atacgatgac tcaaatatgg
     1021 gccagaacaa ggttggcctg tccttcagtt taccgatcta ccagggcggg atggtgaata
     1081 gtcaagtaaa acaggcgcag tataactttg tcggtgcatc agagcaactg gaatcagctc
     1141 accgtagcgt cgtacagact gtccggagta gctttaacaa cattaacgcg agtatctcat
     1201 caatcaatgc ttacaagcaa gcagtggttt ctgcgcagtc ttctttggat gccatggaag
     1261 caggctattc agtgggtact cgcacgattg ttgatgttct cgacgctacc acaactctct
     1321 acaacgcgaa acaggaatta gcgaatgccc gttataacta cttaattaac caactgaata
     1381 tcaaatctgc cctaggtact ctgaatgagc aagacttgct tgcgttaaat aacgccctgt
     1441 ccaaacctgt ctcaaccaat cctgagaacg tagcacctca gacccctgaa cagaacgcca
     1501 ttgcggacgg ttacgcccct gacagtcctg cgcctgtggt ccagcagacg tcagcgcgca
     1561 ctaccacttc aaacgggcat aatccgttcc gcaattaata a
//