LOCUS       BBa_K1529230             901 bp    DNA     linear       27-Dec-2024
DEFINITION  Ptet_M256ICysE
ACCESSION   BBa_K1529230
VERSION     BBa_K1529230.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..68
                     /label=RBS
     misc_feature    69..76
                     /label=Spacer
     misc_feature    77..79
                     /label=Start
     misc_feature    896..901
                     /label=Stop
     CDS             63..901
                     /label=BBa_K731010
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaggaggtt tttattatgt cgtgtgaaga actggaaatt gtctggaaca atattaaagc
      121 cgaagccaga acgctggcgg actgtgagcc aatgctggcc agtttttacc acgcgacgct
      181 actcaagcac gaaaaccttg gcagtgcact gagctacatg ctggcgaaca agctgtcatc
      241 gccaattatg cctgctattg ctatccgtga agtggtggaa gaagcctacg ccgctgaccc
      301 ggaaatgatc gcctctgcgg cctgtgatat tcaggcggtg cgtacccgcg acccggcagt
      361 cgataaatac tcaaccccgt tgttatacct gaagggtttt catgccttgc aggcctatcg
      421 catcggtcac tggttgtgga atcaggggcg tcgcgcactg gcaatctttc tgcaaaacca
      481 ggtttctgtg acgttccagg tcgatattca cccggcagca aaaattggtc gcggtatcat
      541 gcttgaccac gcgacaggca tcgtcgttgg tgaaacggcg gtgattgaaa acgacgtatc
      601 gattctgcaa tctgtgacgc ttggcggtac gggtaaatct ggtggtgacc gtcacccgaa
      661 aattcgtgaa ggtgtgatga ttggcgcggg cgcgaaaatc ctcggcaata ttgaagttgg
      721 gcgcggcgcg aagattggcg caggttccgt ggtgctgcaa ccggtgccgc cgcataccac
      781 cgccgctggc gttccggctc gtattgtcgg taaaccagac agcgataagc catcaatgga
      841 tatcgaccag catttcaacg gtattaacca tacatttgag tatggggatg ggatctaata
      901 a
//