##gff-version 3
##sequence-region BBa_K1529230 1 901
BBa_K1529230	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K1529230	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1529230	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1529230	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1529230	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1529230	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1529230	.	CDS	63	901	.	+	0	ID=BBa_K731010;Name=BBa_K731010
BBa_K1529230	.	sequence_feature	69	76	.	+	0	ID=annotation2180202;Name=Spacer;Parent=BBa_K731010
BBa_K1529230	.	stop_codon	896	901	.	+	0	ID=annotation2180205;Name=Stop;Parent=BBa_K731010
BBa_K1529230	.	start_codon	77	79	.	+	0	ID=annotation2180204;Name=Start;Parent=BBa_K731010
BBa_K1529230	.	ribosome_entry_site	63	68	.	+	0	ID=annotation2180201;Name=RBS;Parent=BBa_K731010
>BBa_K1529230
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaggaggtttttattatg
tcgtgtgaagaactggaaattgtctggaacaatattaaagccgaagccagaacgctggcggactgtgagccaatgctgg
ccagtttttaccacgcgacgctactcaagcacgaaaaccttggcagtgcactgagctacatgctggcgaacaagctgtc
atcgccaattatgcctgctattgctatccgtgaagtggtggaagaagcctacgccgctgacccggaaatgatcgcctct
gcggcctgtgatattcaggcggtgcgtacccgcgacccggcagtcgataaatactcaaccccgttgttatacctgaagg
gttttcatgccttgcaggcctatcgcatcggtcactggttgtggaatcaggggcgtcgcgcactggcaatctttctgca
aaaccaggtttctgtgacgttccaggtcgatattcacccggcagcaaaaattggtcgcggtatcatgcttgaccacgcg
acaggcatcgtcgttggtgaaacggcggtgattgaaaacgacgtatcgattctgcaatctgtgacgcttggcggtacgg
gtaaatctggtggtgaccgtcacccgaaaattcgtgaaggtgtgatgattggcgcgggcgcgaaaatcctcggcaatat
tgaagttgggcgcggcgcgaagattggcgcaggttccgtggtgctgcaaccggtgccgccgcataccaccgccgctggc
gttccggctcgtattgtcggtaaaccagacagcgataagccatcaatggatatcgaccagcatttcaacggtattaacc
atacatttgagtatggggatgggatctaataa