LOCUS       BBa_K1529235            1908 bp    DNA     linear       01-Jun-2024
DEFINITION  Plux_M256ICysE_Ptet_luxR_TT
ACCESSION   BBa_K1529235
VERSION     BBa_K1529235.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        20..25
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    53..53
                     /label=start
     misc_feature    1..55
                     /label=BBa_R0062
     promoter        1..55
                     /label=BBa_R0062
     RBS             64..69
                     /label=RBS
     misc_feature    70..77
                     /label=Spacer
     misc_feature    78..80
                     /label=Start
     misc_feature    897..902
                     /label=Stop
     CDS             64..902
                     /label=BBa_K731010
     misc_feature    911..929
                     /label=TetR 1
     misc_feature    911..964
                     /label=BBa_R0040
     promoter        930..935
                     /label=-35
     misc_feature    936..954
                     /label=TetR 2
     promoter        953..958
                     /label=-10
     promoter        911..964
                     /label=BBa_R0040
     misc_feature    977..980
                     /label=conserved
     RBS             973..984
                     /label=BBa_B0034
     misc_feature    991..992
                     /label=prefix
     misc_feature    1164..1164
                     /label=T
     misc_feature    1482..1482
                     /label=A
     CDS             991..1740
                     /label=luxR
     misc_feature    991..1746
                     /label=BBa_C0062
     misc_feature    1747..1771
                     /label=Help:Barcodes
     CDS             991..1746
                     /label=BBa_C0062
     stem_loop       1791..1834
                     /label=stem_loop
     misc_feature    1780..1859
                     /label=BBa_B0010
     terminator      1780..1859
                     /label=BBa_B0010
     stem_loop       1875..1894
                     /label=T7 TE
     misc_feature    1901..1901
                     /label=stop
     polyA_site      1895..1908
                     /label=polya
     misc_feature    1868..1908
                     /label=BBa_B0012
     terminator      1868..1908
                     /label=BBa_B0012
     terminator      1780..1908
                     /label=BBa_B0015
ORIGIN
        1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta
       61 gagaggaggt ttttattatg tcgtgtgaag aactggaaat tgtctggaac aatattaaag
      121 ccgaagccag aacgctggcg gactgtgagc caatgctggc cagtttttac cacgcgacgc
      181 tactcaagca cgaaaacctt ggcagtgcac tgagctacat gctggcgaac aagctgtcat
      241 cgccaattat gcctgctatt gctatccgtg aagtggtgga agaagcctac gccgctgacc
      301 cggaaatgat cgcctctgcg gcctgtgata ttcaggcggt gcgtacccgc gacccggcag
      361 tcgataaata ctcaaccccg ttgttatacc tgaagggttt tcatgccttg caggcctatc
      421 gcatcggtca ctggttgtgg aatcaggggc gtcgcgcact ggcaatcttt ctgcaaaacc
      481 aggtttctgt gacgttccag gtcgatattc acccggcagc aaaaattggt cgcggtatca
      541 tgcttgacca cgcgacaggc atcgtcgttg gtgaaacggc ggtgattgaa aacgacgtat
      601 cgattctgca atctgtgacg cttggcggta cgggtaaatc tggtggtgac cgtcacccga
      661 aaattcgtga aggtgtgatg attggcgcgg gcgcgaaaat cctcggcaat attgaagttg
      721 ggcgcggcgc gaagattggc gcaggttccg tggtgctgca accggtgccg ccgcatacca
      781 ccgccgctgg cgttccggct cgtattgtcg gtaaaccaga cagcgataag ccatcaatgg
      841 atatcgacca gcatttcaac ggtattaacc atacatttga gtatggggat gggatctaat
      901 aatactagag tccctatcag tgatagagat tgacatccct atcagtgata gagatactga
      961 gcactactag agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac
     1021 agaataatta ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct
     1081 gatatgacta aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct
     1141 atggttaaat ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat
     1201 gatgacgcta atttaataaa atatgatcct atagtagatt attctaactc caatcattca
     1261 ccaattaatt ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt
     1321 aaagaagcga aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac
     1381 aatggcttcg gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta
     1441 tttttacatg cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga
     1501 aaaataaata tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt
     1561 ttagcgtggg catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt
     1621 gagcgtactg tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc
     1681 tgccaaagta tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat
     1741 taataacact gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa
     1801 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     1861 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//