##gff-version 3
##sequence-region BBa_K1529235 1 1908
BBa_K1529235	.	ribosome_entry_site	973	984	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1529235	.	conserved_region	977	980	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1529235	.	terminator	1780	1908	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1529235	.	terminator	1780	1859	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1529235	.	engineered_region	1780	1859	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1529235	.	stem_loop	1791	1834	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1529235	.	terminator	1868	1908	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1529235	.	stop_codon	1901	1901	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1529235	.	polyA_site	1895	1908	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1529235	.	stem_loop	1875	1894	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1529235	.	engineered_region	1868	1908	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1529235	.	promoter	1	55	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_K1529235	.	start_codon	53	53	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K1529235	.	promoter	42	47	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K1529235	.	promoter	20	25	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K1529235	.	engineered_region	1	55	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K1529235	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K1529235	.	promoter	911	964	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K1529235	.	engineered_region	911	964	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1529235	.	promoter	930	935	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1529235	.	promoter	953	958	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1529235	.	non_covalent_binding_site	911	929	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1529235	.	non_covalent_binding_site	936	954	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1529235	.	CDS	64	902	.	+	0	ID=BBa_K731010;Name=BBa_K731010
BBa_K1529235	.	start_codon	78	80	.	+	0	ID=annotation2180204;Name=Start;Parent=BBa_K731010
BBa_K1529235	.	sequence_feature	70	77	.	+	0	ID=annotation2180202;Name=Spacer;Parent=BBa_K731010
BBa_K1529235	.	ribosome_entry_site	64	69	.	+	0	ID=annotation2180201;Name=RBS;Parent=BBa_K731010
BBa_K1529235	.	stop_codon	897	902	.	+	0	ID=annotation2180205;Name=Stop;Parent=BBa_K731010
BBa_K1529235	.	CDS	991	1746	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_K1529235	.	engineered_tag	1747	1771	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K1529235	.	engineered_region	991	1746	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K1529235	.	engineered_region	991	992	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K1529235	.	point_mutation	1164	1164	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K1529235	.	CDS	991	1740	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K1529235	.	point_mutation	1482	1482	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
>BBa_K1529235
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaggaggtttttattat
gtcgtgtgaagaactggaaattgtctggaacaatattaaagccgaagccagaacgctggcggactgtgagccaatgctg
gccagtttttaccacgcgacgctactcaagcacgaaaaccttggcagtgcactgagctacatgctggcgaacaagctgt
catcgccaattatgcctgctattgctatccgtgaagtggtggaagaagcctacgccgctgacccggaaatgatcgcctc
tgcggcctgtgatattcaggcggtgcgtacccgcgacccggcagtcgataaatactcaaccccgttgttatacctgaag
ggttttcatgccttgcaggcctatcgcatcggtcactggttgtggaatcaggggcgtcgcgcactggcaatctttctgc
aaaaccaggtttctgtgacgttccaggtcgatattcacccggcagcaaaaattggtcgcggtatcatgcttgaccacgc
gacaggcatcgtcgttggtgaaacggcggtgattgaaaacgacgtatcgattctgcaatctgtgacgcttggcggtacg
ggtaaatctggtggtgaccgtcacccgaaaattcgtgaaggtgtgatgattggcgcgggcgcgaaaatcctcggcaata
ttgaagttgggcgcggcgcgaagattggcgcaggttccgtggtgctgcaaccggtgccgccgcataccaccgccgctgg
cgttccggctcgtattgtcggtaaaccagacagcgataagccatcaatggatatcgaccagcatttcaacggtattaac
catacatttgagtatggggatgggatctaataatactagagtccctatcagtgatagagattgacatccctatcagtga
tagagatactgagcactactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataa
ttaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtga
atattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaa
tggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaa
ttaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtct
tatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagac
aactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaa
aaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaagg
aaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatg
aaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaa
attaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagac
tgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctt
tctgcgtttata