LOCUS       BBa_K1529241            1841 bp    DNA     linear       23-Dec-2024
DEFINITION  Plac_cIλ_aiiA
ACCESSION   BBa_K1529241
VERSION     BBa_K1529241.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             230..937
                     /label=cI lambda
     misc_feature    938..970
                     /label=LVA
     misc_feature    977..1001
                     /label=Help:Barcodes
     CDS             227..1001
                     /label=BBa_C0051
     misc_feature    1014..1017
                     /label=conserved
     RBS             1010..1021
                     /label=BBa_B0034
     misc_feature    1028..1030
                     /label=start
     misc_feature    1811..1816
                     /label=2
     misc_feature    1778..1810
                     /label=LVA
     CDS             1028..1777
                     /label=aiiA
     misc_feature    1028..1816
                     /label=BBa_C0060
     misc_feature    1817..1841
                     /label=Help:Barcodes
     CDS             1028..1816
                     /label=BBa_C0060
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga gcacaaaaaa
      241 gaaaccatta acacaagagc agcttgagga cgcacgtcgc cttaaagcaa tttatgaaaa
      301 aaagaaaaat gaacttggct tatcccagga atctgtcgca gacaagatgg ggatggggca
      361 gtcaggcgtt ggtgctttat ttaatggcat caatgcatta aatgcttata acgccgcatt
      421 gcttgcaaaa attctcaaag ttagcgttga agaatttagc ccttcaatcg ccagagaaat
      481 ctacgagatg tatgaagcgg ttagtatgca gccgtcactt agaagtgagt atgagtaccc
      541 tgttttttct catgttcagg cagggatgtt ctcacctgag cttagaacct ttaccaaagg
      601 tgatgcggag agatgggtaa gcacaaccaa aaaagccagt gattctgcat tctggcttga
      661 ggttgaaggt aattccatga ccgcaccaac aggctccaag ccaagctttc ctgacggaat
      721 gttaattctc gttgaccctg agcaggctgt tgagccaggt gatttctgca tagccagact
      781 tgggggtgat gagtttacct tcaagaaact gatcagggat agcggtcagg tgtttttaca
      841 accactaaac ccacagtacc caatgatccc atgcaatgag agttgttccg ttgtggggaa
      901 agttatcgct agtcagtggc ctgaagagac gtttggcgct gcaaacgacg aaaactacgc
      961 tttagtagct taataacgct gatagtgcta gtgtagatcg ctactagaga aagaggagaa
     1021 atactagatg acagtaaaga agctttattt cgtcccagca ggtcgttgta tgttggatca
     1081 ttcgtctgtt aatagtacat taacaccagg agaattatta gacttaccgg tttggtgtta
     1141 tcttttggag actgaagaag gacctatttt agtagataca ggtatgccag aaagtgcagt
     1201 taataatgaa ggtcttttta acggtacatt tgtcgaaggg caggttttac cgaaaatgac
     1261 tgaagaagat agaatcgtga atattttaaa acgggttggt tatgagccgg aagaccttct
     1321 ttatattatt agttctcact tgcattttga tcatgcagga ggaaatggcg cttttataaa
     1381 tacaccaatc attgtacagc gtgctgaata tgaggcggcg cagcatagcg aagaatattt
     1441 gaaagaatgt atattgccga atttaaacta caaaatcatt gaaggtgatt atgaagtcgt
     1501 accaggagtt caattattgc atacaccagg ccatactcca gggcatcaat cgctattaat
     1561 tgagacagaa aaatccggtc ctgtattatt aacgattgat gcatcgtata cgaaagagaa
     1621 ttttgaaaat gaagtgccat ttgcgggatt tgattcagaa ttagctttat cttcaattaa
     1681 acgtttaaaa gaagtggtga tgaaagagaa gccgattgtt ttctttggac atgatataga
     1741 gcaggaaagg ggatgtaaag tgttccctga atatatagct gcaaacgacg aaaactacgc
     1801 tttagtagct taataacgct gatagtgcta gtgtagatcg c
//