##gff-version 3
##sequence-region BBa_K1529271 1 1511
BBa_K1529271	.	ribosome_entry_site	876	887	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1529271	.	conserved_region	880	883	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1529271	.	CDS	894	1511	.	+	0	ID=BBa_C0061;Name=BBa_C0061
BBa_K1529271	.	CDS	894	1472	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K1529271	.	engineered_tag	1512	1536	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K1529271	.	engineered_region	894	1511	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K1529271	.	tag	1473	1504	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K1529271	.	engineered_region	1	867	.	+	0	ID=BBa_K1529270;Name=BBa_K1529270
BBa_K1529271	.	CDS	81	867	.	+	0	ID=BBa_C0071;Name=BBa_C0071;Parent=BBa_K1529270
BBa_K1529271	.	CDS	81	803	.	+	0	ID=annotation301192;Name=rhlR;Parent=BBa_C0071
BBa_K1529271	.	tag	804	836	.	+	0	ID=annotation306523;Name=LVA;Parent=BBa_C0071
BBa_K1529271	.	engineered_tag	843	867	.	+	0	ID=annotation2213995;Name=Help:Barcodes;Parent=BBa_C0071
BBa_K1529271	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1529270
BBa_K1529271	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1529271	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K1529270
BBa_K1529271	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1529271	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1529271	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1529271	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1529271	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
>BBa_K1529271
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgaggaatgacggaggctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggc
gtgttcgccgtcctggaaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattccct
tcacccggccgaagaccgaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggc
cgtggatccggcgatcctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccgg
atgctctggaacgaggctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctca
gcgtgctttccgtggcgcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcat
gatcgagttgctgacccagaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgc
gaacgcgagatcctgcaatggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagca
cggtgaacttccaccacaagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggc
gctgggtctcatcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcacta
ctagagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtata
aaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatc
agatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgttta
ttacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatccta
atatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaa
actatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagag
cgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcgg
ttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagt
agcttaataactctgatagtgctagtgtagatctc