LOCUS       BBa_K1529953             967 bp    DNA     linear       23-Dec-2024
DEFINITION  K1139200 B0030 E0040 B0010 B0012
ACCESSION   BBa_K1529953
VERSION     BBa_K1529953.1  
FEATURES             Location/Qualifiers
     misc_feature    21..39
                     /label=phoB-Phosphorylated binding site
     promoter        48..53
                     /label=-10
     promoter        23..28
                     /label=-35
     promoter        1..81
                     /label=BBa_K1139200
     RBS             90..104
                     /label=RBS-1\Strong
     RBS             97..101
                     /label=RBS
     misc_feature    90..104
                     /label=BBa_B0030
     RBS             90..104
                     /label=BBa_B0030
     CDS             111..830
                     /label=GFP protein
     CDS             111..830
                     /label=BBa_E0040
     stem_loop       850..893
                     /label=stem_loop
     misc_feature    839..918
                     /label=BBa_B0010
     terminator      839..918
                     /label=BBa_B0010
     stem_loop       934..953
                     /label=T7 TE
     misc_feature    960..960
                     /label=stop
     polyA_site      954..967
                     /label=polya
     misc_feature    927..967
                     /label=BBa_B0012
     terminator      927..967
                     /label=BBa_B0012
ORIGIN
        1 aaagttaatc ttttcaacag ctgtcataaa gttgtcacgg ccgagactta tagtcgcttt
       61 gtttttattt tttaatgtat ttactagaga ttaaagagga gaaatactag atgcgtaaag
      121 gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg
      181 ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc
      241 ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactt
      301 tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag catgactttt
      361 tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg
      421 ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg
      481 agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca
      541 actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagtta
      601 acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac
      661 aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac
      721 aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg
      781 taacagctgc tgggattaca catggcatgg atgaactata caaataataa tactagagcc
      841 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
      901 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
      961 gtttata
//