BBa_K1531004
1
BBa_K1531004
Bovine beta casein (B variant) with full alpha-factor yeast secretion signal
2014-10-12T11:00:00Z
2015-05-08T01:10:51Z
Bos taurus sequence, from http://www.ncbi.nlm.nih.gov/protein/AAI11173.1
Beta casein is one of four major caseins (cheese proteins) found in bovine cheese. Together with Kappa casein, it is necessary and sufficient to create casein micelles.
At least 12 different genetic variants of bovine Beta casein have been identified and characterized. The B allele used here is associated with improved milk coagulation.
The coding sequence for this gene is preceded by a secretion signal derived from the yeast mating pheromone alpha-factor in Saccharomyces cerevisiae, facilitates secretion of heterologous proteins in yeast. We used a full length form of the full alpha-factor protein with kex and ste13 protease cleavage sites (FAKS). (www.pnas.org/content/81/15/4642.short). In addition, the entire construct is flanked by SapI restriction sites, for cloning into DNA2.0's Electra system.
false
false
_1913_
0
11141
9
It's complicated
false
The DNA sequence was codon optimized for expression in S. cerevisiae, using IDT's codon optimization tools, followed by manual editing of a few codons to avoid specific restriction sites.
false
Patrik D
annotation2418013
1
start codon
range2418013
1
9
11
annotation2418015
1
bovine Beta casein
range2418015
1
276
905
annotation2418016
1
stop codon
range2418016
1
903
905
annotation2418014
1
alpha factor yeast secretion signal
range2418014
1
12
275
annotation2418012
1
Sap1
range2418012
1
1
11
annotation2418017
1
SapI
range2418017
1
906
916
BBa_K1531004_sequence
1
gctcttctatgagattcccatctattttcaccgctgtcttgttcgctgcctcctctgcattggctgcccctgttaacactaccactgaagacgagactgctcaaattccagctgaagcagttatcggttactctgaccttgagggtgatttcgacgtcgctgttttgcctttctctaactccactaacaacggtttgttgttcattaacaccactatcgcttccattgctgctaaggaagagggtgtctctctcgagaaaagagaggccgaagctagagaattggaagaactaaacgtgcccggggaaatagtcgaatccctttcttcaagcgaagaatcaattacaagaattaacaagaagattgaaaagtttcaatctgaagaacaacagcaaactgaagatgagttgcaggataagattcatccattcgcccaaacacagagtttggtgtatccattccctggtcctatccataattccttgcctcaaaacatccctcccctgacacagactcctgttgttgtcccacctttcctgcaacctgaagttatgggtgtttctaaagtgaaagaagccatggctcctaagcataaagaaatgccatttcctaagtacccagtcgagcccttcacagaaagacagtctttgacactaacagacgtggagaatctgcacttgcctcttccactgcttcaaagctggatgcatcaaccacatcaaccacttccaccaactgtcatgtttcccccacaaagcgtcctaagcttgtctcaaagcaaagtactaccagtaccacaaaaagcggtaccctatcctcagagagatatgcctattcaagcatttctgctttaccaagaacctgtcttaggaccagtaagaggtcccttcccaattattgtgtagggtagaagagc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z