LOCUS       BBa_K1532004            1342 bp    DNA     linear       26-Dec-2024
DEFINITION  Plac->AraC
ACCESSION   BBa_K1532004
VERSION     BBa_K1532004.1  
FEATURES             Location/Qualifiers
     misc_feature    3..8
                     /label=Kpn I cutting site
     misc_feature    1..10
                     /label=BBa_K1532002
     CDS             19..106
                     /label=end of LacI coding region (inactive)
     misc_feature    19..218
                     /label=BBa_R0010
     misc_feature    107..144
                     /label=CAP binding site
     promoter        155..160
                     /label=-35
     promoter        179..184
                     /label=-10
     misc_feature    184..218
                     /label=LacI binding site
     misc_feature    191..191
                     /label=start
     promoter        19..218
                     /label=BBa_R0010
     RBS             227..241
                     /label=RBS-1\Strong
     RBS             234..238
                     /label=RBS
     misc_feature    227..241
                     /label=BBa_B0030
     RBS             227..241
                     /label=BBa_B0030
     CDS             248..1123
                     /label=araC
     misc_feature    1124..1156
                     /label=LVA
     misc_feature    1163..1187
                     /label=Help:Barcodes
     CDS             248..1187
                     /label=BBa_C0080
     stem_loop       1207..1250
                     /label=stem_loop
     misc_feature    1196..1275
                     /label=BBa_B0010
     terminator      1196..1275
                     /label=BBa_B0010
     stem_loop       1291..1310
                     /label=T7 TE
     misc_feature    1317..1317
                     /label=stop
     polyA_site      1311..1324
                     /label=polya
     misc_feature    1284..1324
                     /label=BBa_B0012
     terminator      1284..1324
                     /label=BBa_B0012
     terminator      1196..1324
                     /label=BBa_B0015
     misc_feature    1335..1340
                     /label=Kpn I cutting site
     misc_feature    1333..1342
                     /label=BBa_K1532002
ORIGIN
        1 gcggtaccgg tactagagca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
       61 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
      121 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
      181 tgttgtgtgg aattgtgagc ggataacaat ttcacacata ctagagatta aagaggagaa
      241 atactagatg gctgaagcgc aaaatgatcc cctgctgccg ggatactcgt ttaacgccca
      301 tctggtggcg ggtttaacgc cgattgaggc caatggttat ctcgattttt ttatcgaccg
      361 accgctggga atgaaaggtt atattctcaa tctcaccatt cgcggtcagg gggtggtgaa
      421 aaatcaggga cgagaatttg tctgccgacc gggtgatatt ttgctgttcc cgccaggaga
      481 gattcatcac tacggtcgtc atccggaggc tcgcgaatgg tatcaccagt gggtttactt
      541 tcgtccgcgc gcctactggc atgaatggct taactggccg tcaatatttg ccaatacggg
      601 tttctttcgc ccggatgaag cgcaccagcc gcatttcagc gacctgtttg ggcaaatcat
      661 taacgccggg caaggggaag ggcgctattc ggagctgctg gcgataaatc tgcttgagca
      721 attgttactg cggcgcatgg aagcgattaa cgagtcgctc catccaccga tggataatcg
      781 ggtacgcgag gcttgtcagt acatcagcga tcacctggca gacagcaatt ttgatatcgc
      841 cagcgtcgca cagcatgttt gcctgtcgcc gtcgcgtctg tcacatcttt tccgccagca
      901 gttagggatt agcgtcttaa gctggcgcga ggaccaacgc atcagccagg cgaagctgct
      961 tttgagcact acccggatgc ctatcgccac cgtcggtcgc aatgttggtt ttgacgatca
     1021 actctatttc tcgcgagtat ttaaaaaatg caccggggcc agcccgagcg agttccgtgc
     1081 cggttgtgaa gaaaaagtga atgatgtagc cgtcaagttg tcagctgcaa acgacgaaaa
     1141 ctacgcttta gtagcttaat aacactgata gtgctagtgt agatcactac tagagccagg
     1201 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     1261 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
     1321 tatatactag aggcggtacc gg
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