##gff-version 3
##sequence-region BBa_K1532005 1 1132
BBa_K1532005	.	terminator	986	1114	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1532005	.	terminator	986	1065	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1532005	.	stem_loop	997	1040	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1532005	.	engineered_region	986	1065	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1532005	.	terminator	1074	1114	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1532005	.	engineered_region	1074	1114	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1532005	.	stop_codon	1107	1107	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1532005	.	polyA_site	1101	1114	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1532005	.	stem_loop	1081	1100	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1532005	.	promoter	19	167	.	+	0	ID=BBa_R0080;Name=BBa_R0080
BBa_K1532005	.	non_covalent_binding_site	91	119	.	+	0	ID=annotation301462;Name=ara1 and ara2;Parent=BBa_R0080
BBa_K1532005	.	promoter	131	136	.	+	0	ID=annotation308601;Name=-35;Parent=BBa_R0080
BBa_K1532005	.	operator	24	62	.	+	0	ID=annotation301457;Name=araO1;Parent=BBa_R0080
BBa_K1532005	.	non_covalent_binding_site	22	47	.	+	0	ID=annotation301456;Name=c-amp2;Parent=BBa_R0080
BBa_K1532005	.	non_covalent_binding_site	61	90	.	+	0	ID=annotation301458;Name=c-amp1;Parent=BBa_R0080
BBa_K1532005	.	promoter	154	159	.	+	0	ID=annotation308602;Name=-10;Parent=BBa_R0080
BBa_K1532005	.	CDS	197	952	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_K1532005	.	point_mutation	688	688	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K1532005	.	engineered_region	197	952	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K1532005	.	engineered_region	197	198	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K1532005	.	CDS	197	946	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K1532005	.	point_mutation	370	370	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K1532005	.	engineered_tag	953	977	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K1532005	.	sequence_feature	1	10	.	+	0	ID=BBa_K1532000;Name=BBa_K1532000
BBa_K1532005	.	non_covalent_binding_site	3	8	.	+	0	ID=annotation2391670;Name=ClaI cutting site;Parent=BBa_K1532000
BBa_K1532005	.	ribosome_entry_site	176	190	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K1532005	.	engineered_region	176	190	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1532005	.	ribosome_entry_site	176	190	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1532005	.	ribosome_entry_site	183	187	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1532005	.	sequence_feature	1123	1132	.	+	0	ID=BBa_K1532000;Name=BBa_K1532000
BBa_K1532005	.	non_covalent_binding_site	1125	1130	.	+	0	ID=annotation2391670;Name=ClaI cutting site;Parent=BBa_K1532000
>BBa_K1532005
gcatcgatggtactagaggcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtc
acactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactg
tttctccattactagagattaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaa
taaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatat
tatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatgga
ggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaa
ttggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatc
actgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaact
atatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaat
aaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaa
agctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaac
tcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaatta
ataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactggg
cctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctg
cgtttatatactagaggcatcgatgg