LOCUS BBa_K1532006 1183 bp DNA linear 22-Nov-2024 DEFINITION Plac->LuxR ACCESSION BBa_K1532006 VERSION BBa_K1532006.1 FEATURES Location/Qualifiers misc_feature 3..8 /label=Sal I cutting site misc_feature 1..10 /label=BBa_K1532001 CDS 19..106 /label=end of LacI coding region (inactive) misc_feature 19..218 /label=BBa_R0010 misc_feature 107..144 /label=CAP binding site promoter 155..160 /label=-35 promoter 179..184 /label=-10 misc_feature 184..218 /label=LacI binding site misc_feature 191..191 /label=start promoter 19..218 /label=BBa_R0010 RBS 227..241 /label=RBS-1\Strong RBS 234..238 /label=RBS misc_feature 227..241 /label=BBa_B0030 RBS 227..241 /label=BBa_B0030 misc_feature 248..249 /label=prefix misc_feature 421..421 /label=T misc_feature 739..739 /label=A CDS 248..997 /label=luxR misc_feature 248..1003 /label=BBa_C0062 misc_feature 1004..1028 /label=Help:Barcodes CDS 248..1003 /label=BBa_C0062 stem_loop 1048..1091 /label=stem_loop misc_feature 1037..1116 /label=BBa_B0010 terminator 1037..1116 /label=BBa_B0010 stem_loop 1132..1151 /label=T7 TE misc_feature 1158..1158 /label=stop polyA_site 1152..1165 /label=polya misc_feature 1125..1165 /label=BBa_B0012 terminator 1125..1165 /label=BBa_B0012 terminator 1037..1165 /label=BBa_B0015 misc_feature 1176..1181 /label=Sal I cutting site misc_feature 1174..1183 /label=BBa_K1532001 ORIGIN 1 gcgtcgacgg tactagagca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 61 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta 121 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta 181 tgttgtgtgg aattgtgagc ggataacaat ttcacacata ctagagatta aagaggagaa 241 atactagatg aaaaacataa atgccgacga cacatacaga ataattaata aaattaaagc 301 ttgtagaagc aataatgata ttaatcaatg cttatctgat atgactaaaa tggtacattg 361 tgaatattat ttactcgcga tcatttatcc tcattctatg gttaaatctg atatttcaat 421 cctagataat taccctaaaa aatggaggca atattatgat gacgctaatt taataaaata 481 tgatcctata gtagattatt ctaactccaa tcattcacca attaattgga atatatttga 541 aaacaatgct gtaaataaaa aatctccaaa tgtaattaaa gaagcgaaaa catcaggtct 601 tatcactggg tttagtttcc ctattcatac ggctaacaat ggcttcggaa tgcttagttt 661 tgcacattca gaaaaagaca actatataga tagtttattt ttacatgcgt gtatgaacat 721 accattaatt gttccttctc tagttgataa ttatcgaaaa ataaatatag caaataataa 781 atcaaacaac gatttaacca aaagagaaaa agaatgttta gcgtgggcat gcgaaggaaa 841 aagctcttgg gatatttcaa aaatattagg ttgcagtgag cgtactgtca ctttccattt 901 aaccaatgcg caaatgaaac tcaatacaac aaaccgctgc caaagtattt ctaaagcaat 961 tttaacagga gcaattgatt gcccatactt taaaaattaa taacactgat agtgctagtg 1021 tagatcacta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc 1081 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct 1141 tcgggtgggc ctttctgcgt ttatatacta gaggcgtcga cgg //