##gff-version 3
##sequence-region BBa_K1532006 1 1183
BBa_K1532006	.	sequence_feature	1	10	.	+	0	ID=BBa_K1532001;Name=BBa_K1532001
BBa_K1532006	.	non_covalent_binding_site	3	8	.	+	0	ID=annotation2391710;Name=Sal I cutting site;Parent=BBa_K1532001
BBa_K1532006	.	sequence_feature	1174	1183	.	+	0	ID=BBa_K1532001;Name=BBa_K1532001
BBa_K1532006	.	non_covalent_binding_site	1176	1181	.	+	0	ID=annotation2391710;Name=Sal I cutting site;Parent=BBa_K1532001
BBa_K1532006	.	ribosome_entry_site	227	241	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K1532006	.	ribosome_entry_site	227	241	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1532006	.	ribosome_entry_site	234	238	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1532006	.	engineered_region	227	241	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1532006	.	promoter	19	218	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K1532006	.	start_codon	191	191	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1532006	.	CDS	19	106	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1532006	.	promoter	179	184	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1532006	.	non_covalent_binding_site	184	218	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1532006	.	non_covalent_binding_site	107	144	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1532006	.	engineered_region	19	218	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1532006	.	promoter	155	160	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1532006	.	terminator	1037	1165	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1532006	.	terminator	1125	1165	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1532006	.	polyA_site	1152	1165	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1532006	.	engineered_region	1125	1165	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1532006	.	stem_loop	1132	1151	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1532006	.	stop_codon	1158	1158	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1532006	.	terminator	1037	1116	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1532006	.	stem_loop	1048	1091	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1532006	.	engineered_region	1037	1116	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1532006	.	CDS	248	1003	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_K1532006	.	CDS	248	997	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K1532006	.	engineered_tag	1004	1028	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K1532006	.	engineered_region	248	1003	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K1532006	.	point_mutation	421	421	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K1532006	.	engineered_region	248	249	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K1532006	.	point_mutation	739	739	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
>BBa_K1532006
gcgtcgacggtactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacag
gtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggcttta
cactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagattaaagagga
gaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataat
gatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcatt
ctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaat
aaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgta
aataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacgg
ctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtg
tatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaac
gatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattag
gttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtat
ttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatca
ctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggt
gaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcgtcgacgg