LOCUS BBa_K1532008 5979 bp DNA linear 30-Dec-2025 DEFINITION project system we used to slove the SPP ACCESSION BBa_K1532008 VERSION BBa_K1532008.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 20..25 /label=-35 promoter 42..47 /label=-10 misc_feature 53..53 /label=start misc_feature 1..55 /label=BBa_R0062 promoter 1..55 /label=BBa_R0062 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 CDS 82..801 /label=GFP protein CDS 82..801 /label=BBa_E0040 stem_loop 821..864 /label=stem_loop misc_feature 810..889 /label=BBa_B0010 terminator 810..889 /label=BBa_B0010 stem_loop 905..924 /label=T7 TE misc_feature 931..931 /label=stop polyA_site 925..938 /label=polya misc_feature 898..938 /label=BBa_B0012 terminator 898..938 /label=BBa_B0012 terminator 810..938 /label=BBa_B0015 misc_feature 1..938 /label=BBa_K1532003 misc_feature 949..954 /label=Kpn I cutting site misc_feature 947..956 /label=BBa_K1532002 CDS 965..1052 /label=end of LacI coding region (inactive) misc_feature 965..1164 /label=BBa_R0010 misc_feature 1053..1090 /label=CAP binding site promoter 1101..1106 /label=-35 promoter 1125..1130 /label=-10 misc_feature 1130..1164 /label=LacI binding site misc_feature 1137..1137 /label=start promoter 965..1164 /label=BBa_R0010 RBS 1173..1187 /label=RBS-1\Strong RBS 1180..1184 /label=RBS misc_feature 1173..1187 /label=BBa_B0030 RBS 1173..1187 /label=BBa_B0030 CDS 1194..2069 /label=araC misc_feature 2070..2102 /label=LVA misc_feature 2109..2133 /label=Help:Barcodes CDS 1194..2133 /label=BBa_C0080 stem_loop 2153..2196 /label=stem_loop misc_feature 2142..2221 /label=BBa_B0010 terminator 2142..2221 /label=BBa_B0010 stem_loop 2237..2256 /label=T7 TE misc_feature 2263..2263 /label=stop polyA_site 2257..2270 /label=polya misc_feature 2230..2270 /label=BBa_B0012 terminator 2230..2270 /label=BBa_B0012 terminator 2142..2270 /label=BBa_B0015 misc_feature 2281..2286 /label=Kpn I cutting site misc_feature 2279..2288 /label=BBa_K1532002 misc_feature 947..2288 /label=BBa_K1532004 misc_feature 2299..2304 /label=ClaI cutting site misc_feature 2297..2306 /label=BBa_K1532000 misc_feature 2318..2343 /label=c-amp2 misc_feature 2320..2358 /label=araO1 misc_feature 2357..2386 /label=c-amp1 misc_feature 2387..2415 /label=ara1 and ara2 promoter 2427..2432 /label=-35 promoter 2450..2455 /label=-10 promoter 2315..2463 /label=BBa_R0080 RBS 2472..2486 /label=RBS-1\Strong RBS 2479..2483 /label=RBS misc_feature 2472..2486 /label=BBa_B0030 RBS 2472..2486 /label=BBa_B0030 misc_feature 2493..2494 /label=prefix misc_feature 2666..2666 /label=T misc_feature 2984..2984 /label=A CDS 2493..3242 /label=luxR misc_feature 2493..3248 /label=BBa_C0062 misc_feature 3249..3273 /label=Help:Barcodes CDS 2493..3248 /label=BBa_C0062 stem_loop 3293..3336 /label=stem_loop misc_feature 3282..3361 /label=BBa_B0010 terminator 3282..3361 /label=BBa_B0010 stem_loop 3377..3396 /label=T7 TE misc_feature 3403..3403 /label=stop polyA_site 3397..3410 /label=polya misc_feature 3370..3410 /label=BBa_B0012 terminator 3370..3410 /label=BBa_B0012 terminator 3282..3410 /label=BBa_B0015 misc_feature 3421..3426 /label=ClaI cutting site misc_feature 3419..3428 /label=BBa_K1532000 misc_feature 2297..3428 /label=BBa_K1532005 misc_feature 3439..3444 /label=Sal I cutting site misc_feature 3437..3446 /label=BBa_K1532001 CDS 3455..3542 /label=end of LacI coding region (inactive) misc_feature 3455..3654 /label=BBa_R0010 misc_feature 3543..3580 /label=CAP binding site promoter 3591..3596 /label=-35 promoter 3615..3620 /label=-10 misc_feature 3620..3654 /label=LacI binding site misc_feature 3627..3627 /label=start promoter 3455..3654 /label=BBa_R0010 RBS 3663..3677 /label=RBS-1\Strong RBS 3670..3674 /label=RBS misc_feature 3663..3677 /label=BBa_B0030 RBS 3663..3677 /label=BBa_B0030 misc_feature 3684..3685 /label=prefix misc_feature 3857..3857 /label=T misc_feature 4175..4175 /label=A CDS 3684..4433 /label=luxR misc_feature 3684..4439 /label=BBa_C0062 misc_feature 4440..4464 /label=Help:Barcodes CDS 3684..4439 /label=BBa_C0062 stem_loop 4484..4527 /label=stem_loop misc_feature 4473..4552 /label=BBa_B0010 terminator 4473..4552 /label=BBa_B0010 stem_loop 4568..4587 /label=T7 TE misc_feature 4594..4594 /label=stop polyA_site 4588..4601 /label=polya misc_feature 4561..4601 /label=BBa_B0012 terminator 4561..4601 /label=BBa_B0012 terminator 4473..4601 /label=BBa_B0015 misc_feature 4612..4617 /label=Sal I cutting site misc_feature 4610..4619 /label=BBa_K1532001 misc_feature 3437..4619 /label=BBa_K1532006 promoter 4628..4662 /label=BBa_J23104 RBS 4671..4683 /label=RBS-3\Weak RBS 4677..4680 /label=RBS misc_feature 4671..4683 /label=BBa_B0032 RBS 4671..4683 /label=BBa_B0032 misc_feature 4628..4683 /label=BBa_K608003 misc_feature 5779..5817 /label=LVA CDS 4690..5817 /label=lacI-LVA misc_feature 4690..5817 /label=BBa_C0012 misc_feature 5818..5842 /label=Help:Barcodes CDS 4690..5817 /label=BBa_C0012 stem_loop 5862..5905 /label=stem_loop misc_feature 5851..5930 /label=BBa_B0010 terminator 5851..5930 /label=BBa_B0010 stem_loop 5946..5965 /label=T7 TE misc_feature 5972..5972 /label=stop polyA_site 5966..5979 /label=polya misc_feature 5939..5979 /label=BBa_B0012 terminator 5939..5979 /label=BBa_B0012 terminator 5851..5979 /label=BBa_B0015 misc_feature 4628..5979 /label=BBa_K1532007 ORIGIN 1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta 61 gagaaagagg agaaatacta gatgcgtaaa ggagaagaac ttttcactgg agttgtccca 121 attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt 181 gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta 241 cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga 301 tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta 361 caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag 421 tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat 481 ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt atacatcatg 541 gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat 601 ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc 661 cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa 721 aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg 781 gatgaactat acaaataata atactagagc caggcatcaa ataaaacgaa aggctcagtc 841 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 901 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagaggcgg taccggtact 961 agagcaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac 1021 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc 1081 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt 1141 gtgagcggat aacaatttca cacatactag agattaaaga ggagaaatac tagatggctg 1201 aagcgcaaaa tgatcccctg ctgccgggat actcgtttaa cgcccatctg gtggcgggtt 1261 taacgccgat tgaggccaat ggttatctcg atttttttat cgaccgaccg ctgggaatga 1321 aaggttatat tctcaatctc accattcgcg gtcagggggt ggtgaaaaat cagggacgag 1381 aatttgtctg ccgaccgggt gatattttgc tgttcccgcc aggagagatt catcactacg 1441 gtcgtcatcc ggaggctcgc gaatggtatc accagtgggt ttactttcgt ccgcgcgcct 1501 actggcatga atggcttaac tggccgtcaa tatttgccaa tacgggtttc tttcgcccgg 1561 atgaagcgca ccagccgcat ttcagcgacc tgtttgggca aatcattaac gccgggcaag 1621 gggaagggcg ctattcggag ctgctggcga taaatctgct tgagcaattg ttactgcggc 1681 gcatggaagc gattaacgag tcgctccatc caccgatgga taatcgggta cgcgaggctt 1741 gtcagtacat cagcgatcac ctggcagaca gcaattttga tatcgccagc gtcgcacagc 1801 atgtttgcct gtcgccgtcg cgtctgtcac atcttttccg ccagcagtta gggattagcg 1861 tcttaagctg gcgcgaggac caacgcatca gccaggcgaa gctgcttttg agcactaccc 1921 ggatgcctat cgccaccgtc ggtcgcaatg ttggttttga cgatcaactc tatttctcgc 1981 gagtatttaa aaaatgcacc ggggccagcc cgagcgagtt ccgtgccggt tgtgaagaaa 2041 aagtgaatga tgtagccgtc aagttgtcag ctgcaaacga cgaaaactac gctttagtag 2101 cttaataaca ctgatagtgc tagtgtagat cactactaga gccaggcatc aaataaaacg 2161 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 2221 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagaggc 2281 ggtaccggta ctagaggcat cgatggtact agaggcgtaa caaaagtgtc tataatcacg 2341 gcagaaaagt ccacattgat tatttgcacg gcgtcacact ttgctatgcc atagcatttt 2401 tatccataag attagcggat cctacctgac gctttttatc gcaactctct actgtttctc 2461 cattactaga gattaaagag gagaaatact agatgaaaaa cataaatgcc gacgacacat 2521 acagaataat taataaaatt aaagcttgta gaagcaataa tgatattaat caatgcttat 2581 ctgatatgac taaaatggta cattgtgaat attatttact cgcgatcatt tatcctcatt 2641 ctatggttaa atctgatatt tcaatcctag ataattaccc taaaaaatgg aggcaatatt 2701 atgatgacgc taatttaata aaatatgatc ctatagtaga ttattctaac tccaatcatt 2761 caccaattaa ttggaatata tttgaaaaca atgctgtaaa taaaaaatct ccaaatgtaa 2821 ttaaagaagc gaaaacatca ggtcttatca ctgggtttag tttccctatt catacggcta 2881 acaatggctt cggaatgctt agttttgcac attcagaaaa agacaactat atagatagtt 2941 tatttttaca tgcgtgtatg aacataccat taattgttcc ttctctagtt gataattatc 3001 gaaaaataaa tatagcaaat aataaatcaa acaacgattt aaccaaaaga gaaaaagaat 3061 gtttagcgtg ggcatgcgaa ggaaaaagct cttgggatat ttcaaaaata ttaggttgca 3121 gtgagcgtac tgtcactttc catttaacca atgcgcaaat gaaactcaat acaacaaacc 3181 gctgccaaag tatttctaaa gcaattttaa caggagcaat tgattgccca tactttaaaa 3241 attaataaca ctgatagtgc tagtgtagat cactactaga gccaggcatc aaataaaacg 3301 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 3361 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagaggc 3421 atcgatggta ctagaggcgt cgacggtact agagcaatac gcaaaccgcc tctccccgcg 3481 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt 3541 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt 3601 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacatactag 3661 agattaaaga ggagaaatac tagatgaaaa acataaatgc cgacgacaca tacagaataa 3721 ttaataaaat taaagcttgt agaagcaata atgatattaa tcaatgctta tctgatatga 3781 ctaaaatggt acattgtgaa tattatttac tcgcgatcat ttatcctcat tctatggtta 3841 aatctgatat ttcaatccta gataattacc ctaaaaaatg gaggcaatat tatgatgacg 3901 ctaatttaat aaaatatgat cctatagtag attattctaa ctccaatcat tcaccaatta 3961 attggaatat atttgaaaac aatgctgtaa ataaaaaatc tccaaatgta attaaagaag 4021 cgaaaacatc aggtcttatc actgggttta gtttccctat tcatacggct aacaatggct 4081 tcggaatgct tagttttgca cattcagaaa aagacaacta tatagatagt ttatttttac 4141 atgcgtgtat gaacatacca ttaattgttc cttctctagt tgataattat cgaaaaataa 4201 atatagcaaa taataaatca aacaacgatt taaccaaaag agaaaaagaa tgtttagcgt 4261 gggcatgcga aggaaaaagc tcttgggata tttcaaaaat attaggttgc agtgagcgta 4321 ctgtcacttt ccatttaacc aatgcgcaaa tgaaactcaa tacaacaaac cgctgccaaa 4381 gtatttctaa agcaatttta acaggagcaa ttgattgccc atactttaaa aattaataac 4441 actgatagtg ctagtgtaga tcactactag agccaggcat caaataaaac gaaaggctca 4501 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag 4561 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagagg cgtcgacggt 4621 actagagttg acagctagct cagtcctagg tattgtgcta gctactagag tcacacagga 4681 aagtactaga tggtgaatgt gaaaccagta acgttatacg atgtcgcaga gtatgccggt 4741 gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg 4801 cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg cgtggcacaa 4861 caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct ggccctgcac 4921 gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg 4981 gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt gcacaatctt 5041 ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca ggatgccatt 5101 gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc tgaccagaca 5161 cccatcaaca gtattatttt ctcccatgaa gacggtacgc gactgggcgt ggagcatctg 5221 gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg 5281 cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca gccgatagcg 5341 gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca aatgctgaat 5401 gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct gggcgcaatg 5461 cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt gggatacgac 5521 gataccgaag acagctcatg ttatatcccg ccgttaacca ccatcaaaca ggattttcgc 5581 ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag 5641 ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg 5701 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc 5761 cgactggaaa gcgggcaggc tgcaaacgac gaaaactacg ctttagtagc ttaataactc 5821 tgatagtgct agtgtagatc tctactagag ccaggcatca aataaaacga aaggctcagt 5881 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 5941 acactggctc accttcgggt gggcctttct gcgtttata //