BBa_K1532008
type: ComponentDefinition
persistent identity: BBa K1532008
display id: BBa_K1532008
version: 1
was generated by: iGEM to SBOL conversion
description: project system we used to slove the SPP
created: 2014-10-15T11:00:00Z
modified: 2015-05-08T01:10:52Z
mutable provenance: this part was assambled from the devices we built ourselves
owned by: james
owned by: myers
mutable description: This part is the project system we used to slove the SPP. It refers to the coding of "111" in our graph
discontinued: false
dominant: false
group u list: _1914_
m user id: 0
owner id: 21134
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: N/A
star: false
creator: Xinyuan Qiu
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_K1532003
component: BBa_K1532005
component: BBa_K1532004
component: BBa_K1532007
component: BBa_K1532006
sequence annotation: BBa_K1532003
sequence annotation: BBa_K1532004
sequence annotation: BBa_K1532005
sequence annotation: BBa_K1532006
sequence annotation: BBa_K1532007
sequence: https://synbiohub.org/public/igem/BBa_K1532008_sequence/1

BBa_K1532003
type: Component
persistent identity: component2422730
display id: component2422730
version: 1
top level: BBa_K1532008
definition: BBa_K1532003
access: public

BBa_K1532005
type: Component
persistent identity: component2422780
display id: component2422780
version: 1
top level: BBa_K1532008
definition: BBa_K1532005
access: public

BBa_K1532004
type: Component
persistent identity: component2422754
display id: component2422754
version: 1
top level: BBa_K1532008
definition: BBa_K1532004
access: public

BBa_K1532007
type: Component
persistent identity: component2422823
display id: component2422823
version: 1
top level: BBa_K1532008
definition: BBa_K1532007
access: public

BBa_K1532006
type: Component
persistent identity: component2422806
display id: component2422806
version: 1
top level: BBa_K1532008
definition: BBa_K1532006
access: public

BBa_K1532003
type: SequenceAnnotation
persistent identity: annotation2422730
display id: annotation2422730
version: 1
top level: BBa_K1532008
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component: BBa_K1532003

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persistent identity: range2422730
display id: range2422730
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top level: BBa_K1532008
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BBa_K1532004
type: SequenceAnnotation
persistent identity: annotation2422754
display id: annotation2422754
version: 1
top level: BBa_K1532008
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component: BBa_K1532004

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display id: range2422754
version: 1
top level: BBa_K1532008
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end: 2288
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BBa_K1532005
type: SequenceAnnotation
persistent identity: annotation2422780
display id: annotation2422780
version: 1
top level: BBa_K1532008
location: https://synbiohub.org/public/igem/BBa_K1532008/annotation2422780/range2422780/1
component: BBa_K1532005

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display id: range2422780
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BBa_K1532006
type: SequenceAnnotation
persistent identity: annotation2422806
display id: annotation2422806
version: 1
top level: BBa_K1532008
location: https://synbiohub.org/public/igem/BBa_K1532008/annotation2422806/range2422806/1
component: BBa_K1532006

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display id: range2422806
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BBa_K1532007
type: SequenceAnnotation
persistent identity: annotation2422823
display id: annotation2422823
version: 1
top level: BBa_K1532008
location: https://synbiohub.org/public/igem/BBa_K1532008/annotation2422823/range2422823/1
component: BBa_K1532007

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display id: range2422823
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BBa_K1532003
type: ComponentDefinition
persistent identity: BBa K1532003
display id: BBa_K1532003
version: 1
was generated by: iGEM to SBOL conversion
description: Plux->EGFP
created: 2014-09-29T11:00:00Z
modified: 2015-05-08T01:10:52Z
mutable provenance: This part is assembled with standard bio-bricks from the distribution with the standard assembly protocol.
owned by: james
owned by: myers
mutable description: This part is a functional device with the input of LuxR & AHL and the out put of EGFP. The expression of the output signal will be activated if the protein LuxR and the small molecule exists the same time. We also detected a weak background expression of the EGFP in our test.
discontinued: false
dominant: false
group u list: _1914_
m user id: 0
owner id: 21134
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: false
creator: Xinyuan Qiu
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_R0062
component: BBa_E0040
component: BBa_B0034
component: BBa_B0015
sequence annotation: BBa_R0062
sequence annotation: BBa_B0034
sequence annotation: BBa_E0040
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1532003_sequence/1

BBa_R0062
type: Component
persistent identity: component2390565
display id: component2390565
version: 1
top level: BBa_K1532003
definition: lux pR
access: public

BBa_E0040
type: Component
persistent identity: component2390573
display id: component2390573
version: 1
top level: BBa_K1532003
definition: GFP
access: public

BBa_B0034
type: Component
persistent identity: component2390571
display id: component2390571
version: 1
top level: BBa_K1532003
definition: BBa_B0034
access: public

BBa_B0015
type: Component
persistent identity: component2390580
display id: component2390580
version: 1
top level: BBa_K1532003
definition: BBa_B0015
access: public

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2390565
display id: annotation2390565
version: 1
top level: BBa_K1532003
location: https://synbiohub.org/public/igem/BBa_K1532003/annotation2390565/range2390565/1
component: BBa_R0062

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persistent identity: range2390565
display id: range2390565
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top level: BBa_K1532003
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orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2390571
display id: annotation2390571
version: 1
top level: BBa_K1532003
location: https://synbiohub.org/public/igem/BBa_K1532003/annotation2390571/range2390571/1
component: BBa_B0034

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persistent identity: range2390571
display id: range2390571
version: 1
top level: BBa_K1532003
direction: direction/reverse
start: 64
end: 75
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2390573
display id: annotation2390573
version: 1
top level: BBa_K1532003
location: https://synbiohub.org/public/igem/BBa_K1532003/annotation2390573/range2390573/1
component: BBa_E0040

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persistent identity: range2390573
display id: range2390573
version: 1
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direction: direction/reverse
start: 82
end: 801
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2390580
display id: annotation2390580
version: 1
top level: BBa_K1532003
location: https://synbiohub.org/public/igem/BBa_K1532003/annotation2390580/range2390580/1
component: BBa_B0015

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persistent identity: range2390580
display id: range2390580
version: 1
top level: BBa_K1532003
direction: direction/reverse
start: 810
end: 938
orientation: inline

BBa_K1532004
type: ComponentDefinition
persistent identity: BBa K1532004
display id: BBa_K1532004
version: 1
was generated by: iGEM to SBOL conversion
description: Plac->AraC
created: 2014-09-29T11:00:00Z
modified: 2015-05-08T01:10:52Z
mutable provenance: This part is assembled with standard bio-bricks from the distribution with the standard assembly protocol.
owned by: james
owned by: myers
mutable description: This part is a functional device with the input of LacI & IPTG and the out put of AraC. The expression of the output signal will be activated if the protein LacI and the small molecule IPTG exists the same time. This device will not work with the existence of glucose.We also added a Kpn I cutting site at each side of this part.
discontinued: false
dominant: false
group u list: _1914_
m user id: 0
owner id: 21134
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: The bio-brick BBa_R0010 contains a cap-site so the the glucose will affect the expression of this device .So that a glucose-free culture should be used when using this part.
star: false
creator: Xinyuan Qiu
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K1532002
component: BBa_B0030
component: BBa_R0010
component: BBa_B0015
component: BBa_C0080
sequence annotation: BBa_K1532002
sequence annotation: BBa_R0010
sequence annotation: BBa_B0030
sequence annotation: BBa_C0080
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1532004_sequence/1

BBa_K1532002
type: Component
persistent identity: component2390659
display id: component2390659
version: 1
top level: BBa_K1532004
definition: BBa_K1532002
access: public

BBa_B0030
type: Component
persistent identity: component2390668
display id: component2390668
version: 1
top level: BBa_K1532004
definition: BBa_B0030
access: public

BBa_R0010
type: Component
persistent identity: component2390660
display id: component2390660
version: 1
top level: BBa_K1532004
definition: LacI
access: public

BBa_B0015
type: Component
persistent identity: component2390680
display id: component2390680
version: 1
top level: BBa_K1532004
definition: BBa_B0015
access: public

BBa_C0080
type: Component
persistent identity: component2390673
display id: component2390673
version: 1
top level: BBa_K1532004
definition: araC
access: public

BBa_K1532002
type: Component
persistent identity: component2390681
display id: component2390681
version: 1
top level: BBa_K1532004
definition: BBa_K1532002
access: public

BBa_K1532002
type: SequenceAnnotation
persistent identity: annotation2390659
display id: annotation2390659
version: 1
top level: BBa_K1532004
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component: BBa_K1532002

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persistent identity: range2390659
display id: range2390659
version: 1
top level: BBa_K1532004
direction: direction/reverse
start: 1
end: 10
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2390660
display id: annotation2390660
version: 1
top level: BBa_K1532004
location: https://synbiohub.org/public/igem/BBa_K1532004/annotation2390660/range2390660/1
component: BBa_R0010

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type: Range
persistent identity: range2390660
display id: range2390660
version: 1
top level: BBa_K1532004
direction: direction/forward
start: 19
end: 218
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2390668
display id: annotation2390668
version: 1
top level: BBa_K1532004
location: https://synbiohub.org/public/igem/BBa_K1532004/annotation2390668/range2390668/1
component: BBa_B0030

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persistent identity: range2390668
display id: range2390668
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top level: BBa_K1532004
direction: direction/forward
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end: 241
orientation: inline

BBa_C0080
type: SequenceAnnotation
persistent identity: annotation2390673
display id: annotation2390673
version: 1
top level: BBa_K1532004
location: https://synbiohub.org/public/igem/BBa_K1532004/annotation2390673/range2390673/1
component: BBa_C0080

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persistent identity: range2390673
display id: range2390673
version: 1
top level: BBa_K1532004
direction: direction/reverse
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end: 1187
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2390680
display id: annotation2390680
version: 1
top level: BBa_K1532004
location: https://synbiohub.org/public/igem/BBa_K1532004/annotation2390680/range2390680/1
component: BBa_B0015

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display id: range2390680
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direction: direction/reverse
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BBa_K1532002
type: SequenceAnnotation
persistent identity: annotation2390681
display id: annotation2390681
version: 1
top level: BBa_K1532004
location: https://synbiohub.org/public/igem/BBa_K1532004/annotation2390681/range2390681/1
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persistent identity: range2390681
display id: range2390681
version: 1
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direction: direction/reverse
start: 1333
end: 1342
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BBa_K1532005
type: ComponentDefinition
persistent identity: BBa K1532005
display id: BBa_K1532005
version: 1
was generated by: iGEM to SBOL conversion
description: Pbad->LuxR
created: 2014-09-29T11:00:00Z
modified: 2015-05-08T01:10:52Z
mutable provenance: This part is assembled with standard bio-bricks from the distribution with the standard assembly protocol.
owned by: james
owned by: myers
mutable description: This part is a functional device with the input of AraC & arabinose and the out put of the protein LuxR. The expression of the output signal will be activated if the protein AraC and the small molecule arabinose exists the same time. This part may not work when the glucose exists in the culture.
discontinued: false
dominant: false
group u list: _1914_
m user id: 0
owner id: 21134
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: The BBa_R0080 we used in the device contains a cup-site so that the glucose may affect the work of this device. So, glucose-free culture is suggested to be used if you want to use this device.
star: false
creator: Xinyuan Qiu
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_K1532000
component: BBa_B0030
component: BBa_R0080
component: BBa_B0015
component: BBa_C0062
sequence annotation: BBa_K1532000
sequence annotation: BBa_R0080
sequence annotation: BBa_B0030
sequence annotation: BBa_C0062
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1532005_sequence/1

BBa_K1532000
type: Component
persistent identity: component2390682
display id: component2390682
version: 1
top level: BBa_K1532005
definition: BBa_K1532000
access: public

BBa_B0030
type: Component
persistent identity: component2390691
display id: component2390691
version: 1
top level: BBa_K1532005
definition: BBa_B0030
access: public

BBa_R0080
type: Component
persistent identity: component2390689
display id: component2390689
version: 1
top level: BBa_K1532005
definition: AraC
access: public

BBa_B0015
type: Component
persistent identity: component2390705
display id: component2390705
version: 1
top level: BBa_K1532005
definition: BBa_B0015
access: public

BBa_C0062
type: Component
persistent identity: component2390695
display id: component2390695
version: 1
top level: BBa_K1532005
definition: luxr
access: public

BBa_K1532000
type: Component
persistent identity: component2390706
display id: component2390706
version: 1
top level: BBa_K1532005
definition: BBa_K1532000
access: public

BBa_K1532000
type: SequenceAnnotation
persistent identity: annotation2390682
display id: annotation2390682
version: 1
top level: BBa_K1532005
location: https://synbiohub.org/public/igem/BBa_K1532005/annotation2390682/range2390682/1
component: BBa_K1532000

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display id: range2390682
version: 1
top level: BBa_K1532005
direction: direction/reverse
start: 1
end: 10
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BBa_R0080
type: SequenceAnnotation
persistent identity: annotation2390689
display id: annotation2390689
version: 1
top level: BBa_K1532005
location: https://synbiohub.org/public/igem/BBa_K1532005/annotation2390689/range2390689/1
component: BBa_R0080

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persistent identity: range2390689
display id: range2390689
version: 1
top level: BBa_K1532005
direction: direction/reverse
start: 19
end: 167
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2390691
display id: annotation2390691
version: 1
top level: BBa_K1532005
location: https://synbiohub.org/public/igem/BBa_K1532005/annotation2390691/range2390691/1
component: BBa_B0030

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persistent identity: range2390691
display id: range2390691
version: 1
top level: BBa_K1532005
direction: direction/forward
start: 176
end: 190
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation2390695
display id: annotation2390695
version: 1
top level: BBa_K1532005
location: https://synbiohub.org/public/igem/BBa_K1532005/annotation2390695/range2390695/1
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display id: range2390695
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BBa_B0015
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persistent identity: annotation2390705
display id: annotation2390705
version: 1
top level: BBa_K1532005
location: https://synbiohub.org/public/igem/BBa_K1532005/annotation2390705/range2390705/1
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persistent identity: range2390705
display id: range2390705
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direction: direction/reverse
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BBa_K1532000
type: SequenceAnnotation
persistent identity: annotation2390706
display id: annotation2390706
version: 1
top level: BBa_K1532005
location: https://synbiohub.org/public/igem/BBa_K1532005/annotation2390706/range2390706/1
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BBa_K1532006
type: ComponentDefinition
persistent identity: BBa K1532006
display id: BBa_K1532006
version: 1
was generated by: iGEM to SBOL conversion
description: Plac->LuxR
created: 2014-09-29T11:00:00Z
modified: 2015-05-08T01:10:52Z
mutable provenance: This part is assembled with standard bio-bricks from the distribution with the standard assembly protocol.
owned by: myers
owned by: james
mutable description: This part is a functional device with the input of LacI & IPTG and the out put of the protein LuxR. The expression of the output signal will be activated if the protein LacI and the small molecule IPTG exists the same time. This part may not work when the glucose exists in the culture.
discontinued: false
dominant: false
group u list: _1914_
m user id: 0
owner id: 21134
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: The BBa_R0010 we used in the device contains a cup-site so that the glucose may affect the work of this device. So, glucose-free culture is suggested to be used if you want to use this device.
star: false
creator: Xinyuan Qiu
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_B0015
component: BBa_C0062
component: BBa_K1532001
component: BBa_B0030
component: BBa_R0010
sequence annotation: BBa_K1532001
sequence annotation: BBa_R0010
sequence annotation: BBa_B0030
sequence annotation: BBa_C0062
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1532006_sequence/1

BBa_B0015
type: Component
persistent identity: component2390754
display id: component2390754
version: 1
top level: BBa_K1532006
definition: BBa_B0015
access: public

BBa_C0062
type: Component
persistent identity: component2390744
display id: component2390744
version: 1
top level: BBa_K1532006
definition: luxr
access: public

BBa_K1532001
type: Component
persistent identity: component2390755
display id: component2390755
version: 1
top level: BBa_K1532006
definition: BBa_K1532001
access: public

BBa_K1532001
type: Component
persistent identity: component2390731
display id: component2390731
version: 1
top level: BBa_K1532006
definition: BBa_K1532001
access: public

BBa_B0030
type: Component
persistent identity: component2390740
display id: component2390740
version: 1
top level: BBa_K1532006
definition: BBa_B0030
access: public

BBa_R0010
type: Component
persistent identity: component2390732
display id: component2390732
version: 1
top level: BBa_K1532006
definition: LacI
access: public

BBa_K1532001
type: SequenceAnnotation
persistent identity: annotation2390731
display id: annotation2390731
version: 1
top level: BBa_K1532006
location: https://synbiohub.org/public/igem/BBa_K1532006/annotation2390731/range2390731/1
component: BBa_K1532001

https://synbiohub.org/public/igem/BBa_K1532006/annotation2390731/range2390731/1
type: Range
persistent identity: range2390731
display id: range2390731
version: 1
top level: BBa_K1532006
direction: direction/reverse
start: 1
end: 10
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2390732
display id: annotation2390732
version: 1
top level: BBa_K1532006
location: https://synbiohub.org/public/igem/BBa_K1532006/annotation2390732/range2390732/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K1532006/annotation2390732/range2390732/1
type: Range
persistent identity: range2390732
display id: range2390732
version: 1
top level: BBa_K1532006
direction: direction/forward
start: 19
end: 218
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2390740
display id: annotation2390740
version: 1
top level: BBa_K1532006
location: https://synbiohub.org/public/igem/BBa_K1532006/annotation2390740/range2390740/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K1532006/annotation2390740/range2390740/1
type: Range
persistent identity: range2390740
display id: range2390740
version: 1
top level: BBa_K1532006
direction: direction/forward
start: 227
end: 241
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation2390744
display id: annotation2390744
version: 1
top level: BBa_K1532006
location: https://synbiohub.org/public/igem/BBa_K1532006/annotation2390744/range2390744/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_K1532006/annotation2390744/range2390744/1
type: Range
persistent identity: range2390744
display id: range2390744
version: 1
top level: BBa_K1532006
direction: direction/forward
start: 248
end: 1003
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2390754
display id: annotation2390754
version: 1
top level: BBa_K1532006
location: https://synbiohub.org/public/igem/BBa_K1532006/annotation2390754/range2390754/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1532006/annotation2390754/range2390754/1
type: Range
persistent identity: range2390754
display id: range2390754
version: 1
top level: BBa_K1532006
direction: direction/reverse
start: 1037
end: 1165
orientation: inline

BBa_K1532001
type: SequenceAnnotation
persistent identity: annotation2390755
display id: annotation2390755
version: 1
top level: BBa_K1532006
location: https://synbiohub.org/public/igem/BBa_K1532006/annotation2390755/range2390755/1
component: BBa_K1532001

https://synbiohub.org/public/igem/BBa_K1532006/annotation2390755/range2390755/1
type: Range
persistent identity: range2390755
display id: range2390755
version: 1
top level: BBa_K1532006
direction: direction/reverse
start: 1174
end: 1183
orientation: inline

BBa_K1532007
type: ComponentDefinition
persistent identity: BBa K1532007
display id: BBa_K1532007
version: 1
was generated by: iGEM to SBOL conversion
description: LacI Producer
created: 2014-10-03T11:00:00Z
modified: 2015-05-08T01:10:52Z
mutable provenance: This part is assembled with standard bio-bricks from the distribution with the standard assembly protocol
owned by: james
owned by: myers
mutable description: This part contains a LacI CDS led by a constitutive promoter.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1914_
m user id: 0
owner id: 21134
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: N/A
star: false
creator: Xinyuan Qiu, Miyang Li
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_B0015
component: BBa_C0012
component: BBa_K608003
sequence annotation: BBa_K608003
sequence annotation: BBa_C0012
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1532007_sequence/1

BBa_B0015
type: Component
persistent identity: component2393791
display id: component2393791
version: 1
top level: BBa_K1532007
definition: BBa_B0015
access: public

BBa_C0012
type: Component
persistent identity: component2393782
display id: component2393782
version: 1
top level: BBa_K1532007
definition: lacI
access: public

BBa_K608003
type: Component
persistent identity: component2393780
display id: component2393780
version: 1
top level: BBa_K1532007
definition: BBa_K608003
access: public

BBa_K608003
type: SequenceAnnotation
persistent identity: annotation2393780
display id: annotation2393780
version: 1
top level: BBa_K1532007
location: https://synbiohub.org/public/igem/BBa_K1532007/annotation2393780/range2393780/1
component: BBa_K608003

https://synbiohub.org/public/igem/BBa_K1532007/annotation2393780/range2393780/1
type: Range
persistent identity: range2393780
display id: range2393780
version: 1
top level: BBa_K1532007
direction: direction/reverse
start: 1
end: 56
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2393782
display id: annotation2393782
version: 1
top level: BBa_K1532007
location: https://synbiohub.org/public/igem/BBa_K1532007/annotation2393782/range2393782/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_K1532007/annotation2393782/range2393782/1
type: Range
persistent identity: range2393782
display id: range2393782
version: 1
top level: BBa_K1532007
direction: direction/forward
start: 63
end: 1190
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2393791
display id: annotation2393791
version: 1
top level: BBa_K1532007
location: https://synbiohub.org/public/igem/BBa_K1532007/annotation2393791/range2393791/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1532007/annotation2393791/range2393791/1
type: Range
persistent identity: range2393791
display id: range2393791
version: 1
top level: BBa_K1532007
direction: direction/reverse
start: 1224
end: 1352
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

lux pR
type: ComponentDefinition
persistent identity: BBa R0062
display id: BBa_R0062
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (luxR & HSL regulated -- lux pR)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: V. fischeri
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Promoter activated by LuxR in concert with HSL The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <img src="<bb_file>Image1.gif</bb_file>" width="614" height="362">
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: BBa_R0062
sequence annotation: LuxR/HSL
sequence annotation: -35
sequence annotation: -10
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0062_sequence/1

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation7070
display id: annotation7070
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1
type: Range
persistent identity: range7070
display id: range7070
version: 1
top level: lux pR
direction: direction/forward
start: 1
end: 55
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2045
display id: annotation2045
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1
type: Range
persistent identity: range2045
display id: range2045
version: 1
top level: lux pR
direction: direction/forward
start: 1
end: 20
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2046
display id: annotation2046
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1
type: Range
persistent identity: range2046
display id: range2046
version: 1
top level: lux pR
direction: direction/forward
start: 20
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2047
display id: annotation2047
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1
type: Range
persistent identity: range2047
display id: range2047
version: 1
top level: lux pR
direction: direction/forward
start: 42
end: 47
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2048
display id: annotation2048
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1
type: Range
persistent identity: range2048
display id: range2048
version: 1
top level: lux pR
direction: direction/forward
start: 53
end: 53
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

araC
type: ComponentDefinition
persistent identity: BBa C0080
display id: BBa_C0080
version: 1
was generated by: iGEM to SBOL conversion
description: araC arabinose operon regulatory protein (repressor/activator) from E. coli (+LVA)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:24Z
mutable provenance: GenBank: NC_002655 (www.ncbi.nlm.nih.gov)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: coding region for the araC gene, used to make araC protein for use in positive or negative regulation of TIPs output (see also R0080, R0081)
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Sara Neves (Fighting Darwins)
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: araC
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0080_sequence/1

araC
type: SequenceAnnotation
persistent identity: annotation308191
display id: annotation308191
version: 1
top level: araC
location: https://synbiohub.org/public/igem/BBa_C0080/annotation308191/range308191/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0080/annotation308191/range308191/1
type: Range
persistent identity: range308191
display id: range308191
version: 1
top level: araC
direction: direction/forward
start: 1
end: 876
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation308196
display id: annotation308196
version: 1
top level: araC
location: https://synbiohub.org/public/igem/BBa_C0080/annotation308196/range308196/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0080/annotation308196/range308196/1
type: Range
persistent identity: range308196
display id: range308196
version: 1
top level: araC
direction: direction/forward
start: 877
end: 909
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214004
display id: annotation2214004
version: 1
top level: araC
location: https://synbiohub.org/public/igem/BBa_C0080/annotation2214004/range2214004/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0080/annotation2214004/range2214004/1
type: Range
persistent identity: range2214004
display id: range2214004
version: 1
top level: araC
direction: direction/forward
start: 916
end: 940
orientation: inline

BBa_K1532000
type: ComponentDefinition
persistent identity: BBa K1532000
display id: BBa_K1532000
version: 1
was generated by: iGEM to SBOL conversion
description: Cla I cutting site (methylation insensitive)
created: 2014-09-28T11:00:00Z
modified: 2015-05-08T01:10:51Z
mutable provenance: The coding of the Cla I cutting site is open-accessed.
owned by: james
owned by: myers
mutable description: This part is the cutting site of the restriction endonuclease Cla I. The endonuclease Cla I can specifically recognize the sequence of this part and cut the double helix into two cohesive ends. You can use this part when you are trying to add an extra cutting site inside of your system in order to make it open-edited.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1914_
m user id: 0
owner id: 21134
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: The original cutting site is sensitive to methylation, we re-engineered the sequence so that the methylation will not affect the cutting of Cla I.
star: false
creator: Xinyuan Qiu
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence annotation: ClaI cutting site
sequence: https://synbiohub.org/public/igem/BBa_K1532000_sequence/1

ClaI cutting site
type: SequenceAnnotation
persistent identity: annotation2391670
display id: annotation2391670
version: 1
top level: BBa_K1532000
location: https://synbiohub.org/public/igem/BBa_K1532000/annotation2391670/range2391670/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K1532000/annotation2391670/range2391670/1
type: Range
persistent identity: range2391670
display id: range2391670
version: 1
top level: BBa_K1532000
direction: direction/forward
start: 3
end: 8
orientation: inline

BBa_K1532002
type: ComponentDefinition
persistent identity: BBa K1532002
display id: BBa_K1532002
version: 1
was generated by: iGEM to SBOL conversion
description: Kpn I cutting site
created: 2014-09-29T11:00:00Z
modified: 2015-05-08T01:10:51Z
mutable provenance: The coding of the SalI cutting site is open-accessed
owned by: james
owned by: myers
mutable description: This part is the cutting site of the restriction endonuclease Kpn I. The endonuclease Kpn I can specifically recognize the sequence of this part and cut the double helix into two cohesive ends. You can use this part when you are trying to add an extra cutting site inside of your system in order to make it open-edited.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1914_
m user id: 0
owner id: 21134
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: false
creator: Xinyuan Qiu
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence annotation: Kpn I cutting site
sequence: https://synbiohub.org/public/igem/BBa_K1532002_sequence/1

Kpn I cutting site
type: SequenceAnnotation
persistent identity: annotation2391757
display id: annotation2391757
version: 1
top level: BBa_K1532002
location: https://synbiohub.org/public/igem/BBa_K1532002/annotation2391757/range2391757/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K1532002/annotation2391757/range2391757/1
type: Range
persistent identity: range2391757
display id: range2391757
version: 1
top level: BBa_K1532002
direction: direction/forward
start: 3
end: 8
orientation: inline

luxr
type: ComponentDefinition
persistent identity: BBa C0062
display id: BBa_C0062
version: 1
was generated by: iGEM to SBOL conversion
description: luxR repressor/activator, (no LVA?)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: V. fischeri <genbank>AF170104</genbank>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr <bb_part>BBa_R0062</bb_part>, and repressing transcription from Pl <bb_part>BBa_R0063</bb_part>. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: 2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in E. coli with codons used at a similar frequency.
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: luxR
sequence annotation: prefix
sequence annotation: BBa_C0062
sequence annotation: T
sequence annotation: A
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0062_sequence/1

luxR
type: SequenceAnnotation
persistent identity: annotation1766
display id: annotation1766
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
type: Range
persistent identity: range1766
display id: range1766
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 750
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation1762
display id: annotation1762
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
type: Range
persistent identity: range1762
display id: range1762
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 2
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation7039
display id: annotation7039
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
type: Range
persistent identity: range7039
display id: range7039
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 756
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation1764
display id: annotation1764
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
type: Range
persistent identity: range1764
display id: range1764
version: 1
top level: luxr
direction: direction/forward
start: 174
end: 174
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation1765
display id: annotation1765
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
type: Range
persistent identity: range1765
display id: range1765
version: 1
top level: luxr
direction: direction/forward
start: 492
end: 492
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213986
display id: annotation2213986
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
type: Range
persistent identity: range2213986
display id: range2213986
version: 1
top level: luxr
direction: direction/forward
start: 757
end: 781
orientation: inline

lacI
type: ComponentDefinition
persistent identity: BBa C0012
display id: BBa_C0012
version: 1
was generated by: iGEM to SBOL conversion
description: lacI repressor from E. coli (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the LacI protein with an LVA degradation tail and without an RBS. LacI binds to the pLac regulator <bb_part>BBa_R0010</bb_part> and PLlac01 hybrid regulator <bb_part>BBa_R0011</bb_part> and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription. A rapid degredation tail (LVA) has been added to improve the High to Low performance of this part.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] Sequence taken from the repressilator of Elowitz and Leibler (2000). The obtained sequence was compared to the wild-type sequence for LacI obtained through a database search. The sequence had been modified from the wild-type in that wild-type GTG start was changed to an ATG start (note, actual ORF in E.coli has several GTG starts it would seem). The LVA tag has been added for quicker degradation. Incompatible with systems containing LacI, lactose, or IPTG.
star: true
creator: Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: BBa_C0012
sequence annotation: lacI-LVA
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0012_sequence/1

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation7031
display id: annotation7031
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1
type: Range
persistent identity: range7031
display id: range7031
version: 1
top level: lacI
direction: direction/forward
start: 1
end: 1128
orientation: inline

lacI-LVA
type: SequenceAnnotation
persistent identity: annotation1723
display id: annotation1723
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1
type: Range
persistent identity: range1723
display id: range1723
version: 1
top level: lacI
direction: direction/forward
start: 1
end: 1128
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1722
display id: annotation1722
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1
type: Range
persistent identity: range1722
display id: range1722
version: 1
top level: lacI
direction: direction/forward
start: 1090
end: 1128
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213988
display id: annotation2213988
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1
type: Range
persistent identity: range2213988
display id: range2213988
version: 1
top level: lacI
direction: direction/forward
start: 1129
end: 1153
orientation: inline

AraC
type: ComponentDefinition
persistent identity: BBa R0080
display id: BBa_R0080
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (AraC regulated)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: GenBank: J01641 (www.ncbi.nlm.nih.gov)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: AraC operator, truncated to include araO1, araI1, araI2, c-amp1, and c-amp2 sites. This operator should *activate* transcription in the presence of AraC; b/c the operator lacks the araO2 site, there should not be araC-mediated repression.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Sara Neves (Fighting Darwins)
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: c-amp2
sequence annotation: araO1
sequence annotation: c-amp1
sequence annotation: ara1 and ara2
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0080_sequence/1

c-amp2
type: SequenceAnnotation
persistent identity: annotation301456
display id: annotation301456
version: 1
top level: AraC
location: https://synbiohub.org/public/igem/BBa_R0080/annotation301456/range301456/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0080/annotation301456/range301456/1
type: Range
persistent identity: range301456
display id: range301456
version: 1
top level: AraC
direction: direction/forward
start: 4
end: 29
orientation: inline

araO1
type: SequenceAnnotation
persistent identity: annotation301457
display id: annotation301457
version: 1
top level: AraC
location: https://synbiohub.org/public/igem/BBa_R0080/annotation301457/range301457/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0080/annotation301457/range301457/1
type: Range
persistent identity: range301457
display id: range301457
version: 1
top level: AraC
direction: direction/forward
start: 6
end: 44
orientation: inline

c-amp1
type: SequenceAnnotation
persistent identity: annotation301458
display id: annotation301458
version: 1
top level: AraC
location: https://synbiohub.org/public/igem/BBa_R0080/annotation301458/range301458/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0080/annotation301458/range301458/1
type: Range
persistent identity: range301458
display id: range301458
version: 1
top level: AraC
direction: direction/forward
start: 43
end: 72
orientation: inline

ara1 and ara2
type: SequenceAnnotation
persistent identity: annotation301462
display id: annotation301462
version: 1
top level: AraC
location: https://synbiohub.org/public/igem/BBa_R0080/annotation301462/range301462/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0080/annotation301462/range301462/1
type: Range
persistent identity: range301462
display id: range301462
version: 1
top level: AraC
direction: direction/forward
start: 73
end: 101
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation308601
display id: annotation308601
version: 1
top level: AraC
location: https://synbiohub.org/public/igem/BBa_R0080/annotation308601/range308601/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0080/annotation308601/range308601/1
type: Range
persistent identity: range308601
display id: range308601
version: 1
top level: AraC
direction: direction/forward
start: 113
end: 118
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation308602
display id: annotation308602
version: 1
top level: AraC
location: https://synbiohub.org/public/igem/BBa_R0080/annotation308602/range308602/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0080/annotation308602/range308602/1
type: Range
persistent identity: range308602
display id: range308602
version: 1
top level: AraC
direction: direction/forward
start: 136
end: 141
orientation: inline

BBa_K1532001
type: ComponentDefinition
persistent identity: BBa K1532001
display id: BBa_K1532001
version: 1
was generated by: iGEM to SBOL conversion
description: Sal I cutting site
created: 2014-09-29T11:00:00Z
modified: 2015-05-08T01:10:51Z
mutable provenance: The coding of the SalI cutting site is open-accessed
owned by: james
owned by: myers
mutable description: This part is the cutting site of the restriction endonuclease Sal I. The endonuclease Sal I can specifically recognize the sequence of this part and cut the double helix into two cohesive ends. You can use this part when you are trying to add an extra cutting site inside of your system in order to make it open-edited.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1914_
m user id: 0
owner id: 21134
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: false
creator: Xinyuan Qiu
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence annotation: Sal I cutting site
sequence: https://synbiohub.org/public/igem/BBa_K1532001_sequence/1

Sal I cutting site
type: SequenceAnnotation
persistent identity: annotation2391710
display id: annotation2391710
version: 1
top level: BBa_K1532001
location: https://synbiohub.org/public/igem/BBa_K1532001/annotation2391710/range2391710/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K1532001/annotation2391710/range2391710/1
type: Range
persistent identity: range2391710
display id: range2391710
version: 1
top level: BBa_K1532001
direction: direction/forward
start: 3
end: 8
orientation: inline

BBa_K608003
type: ComponentDefinition
persistent identity: BBa K608003
display id: BBa_K608003
version: 1
was generated by: iGEM to SBOL conversion
description: strong Promoter , medium RBS
created: 2011-09-14T11:00:00Z
modified: 2015-05-08T01:12:52Z
mutable provenance: composite
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: strong Promotor , medium RBS
discontinued: false
dominant: false
experience: experience/None
group u list: _780_
m user id: 0
owner id: 9115
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: composite
star: false
creator: Julia M??ller
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_J23104
component: BBa_B0032
sequence annotation: BBa_J23104
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_K608003_sequence/1

BBa_J23104
type: Component
persistent identity: component2128649
display id: component2128649
version: 1
top level: BBa_K608003
definition: BBa_J23104
access: public

BBa_B0032
type: Component
persistent identity: component2128651
display id: component2128651
version: 1
top level: BBa_K608003
definition: BBa_B0032
access: public

BBa_J23104
type: SequenceAnnotation
persistent identity: annotation2128649
display id: annotation2128649
version: 1
top level: BBa_K608003
location: https://synbiohub.org/public/igem/BBa_K608003/annotation2128649/range2128649/1
component: BBa_J23104

https://synbiohub.org/public/igem/BBa_K608003/annotation2128649/range2128649/1
type: Range
persistent identity: range2128649
display id: range2128649
version: 1
top level: BBa_K608003
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2128651
display id: annotation2128651
version: 1
top level: BBa_K608003
location: https://synbiohub.org/public/igem/BBa_K608003/annotation2128651/range2128651/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K608003/annotation2128651/range2128651/1
type: Range
persistent identity: range2128651
display id: range2128651
version: 1
top level: BBa_K608003
direction: direction/forward
start: 44
end: 56
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_J23104
type: ComponentDefinition
persistent identity: BBa J23104
display id: BBa_J23104
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: isolated from library of promoters
owned by: james
owned by: myers
mutable description: replace later
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_J23104_sequence/1

https://synbiohub.org/public/igem/BBa_K1532008_sequence/1
type: Sequence
persistent identity: BBa K1532008 sequence
display id: BBa_K1532008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcggtaccggtactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagattaaagaggagaaatactagatggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgaggccaatggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgtcgccgtcgcgtctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcatcagccaggcgaagctgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcgagtatttaaaaaatgcacc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1532003_sequence/1
type: Sequence
persistent identity: BBa K1532003 sequence
display id: BBa_K1532003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1532004_sequence/1
type: Sequence
persistent identity: BBa K1532004 sequence
display id: BBa_K1532004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcggtaccggtactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagattaaagaggagaaatactagatggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgaggccaatggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgtcgccgtcgcgtctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcatcagccaggcgaagctgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcgagtatttaaaaaatgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcggtaccgg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1532005_sequence/1
type: Sequence
persistent identity: BBa K1532005 sequence
display id: BBa_K1532005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcatcgatggtactagaggcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccattactagagattaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcatcgatgg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1532006_sequence/1
type: Sequence
persistent identity: BBa K1532006 sequence
display id: BBa_K1532006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcgtcgacggtactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagattaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcgtcgacgg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1532007_sequence/1
type: Sequence
persistent identity: BBa K1532007 sequence
display id: BBa_K1532007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtattgtgctagctactagagtcacacaggaaagtactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0062_sequence/1
type: Sequence
persistent identity: BBa R0062 sequence
display id: BBa_R0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0080_sequence/1
type: Sequence
persistent identity: BBa C0080 sequence
display id: BBa_C0080_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgaggccaatggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgtcgccgtcgcgtctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcatcagccaggcgaagctgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcgagtatttaaaaaatgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1532000_sequence/1
type: Sequence
persistent identity: BBa K1532000 sequence
display id: BBa_K1532000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcatcgatgg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1532002_sequence/1
type: Sequence
persistent identity: BBa K1532002 sequence
display id: BBa_K1532002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcggtaccgg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0062_sequence/1
type: Sequence
persistent identity: BBa C0062 sequence
display id: BBa_C0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0012_sequence/1
type: Sequence
persistent identity: BBa C0012 sequence
display id: BBa_C0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0080_sequence/1
type: Sequence
persistent identity: BBa R0080 sequence
display id: BBa_R0080_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1532001_sequence/1
type: Sequence
persistent identity: BBa K1532001 sequence
display id: BBa_K1532001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcgtcgacgg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K608003_sequence/1
type: Sequence
persistent identity: BBa K608003 sequence
display id: BBa_K608003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtattgtgctagctactagagtcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23104_sequence/1
type: Sequence
persistent identity: BBa J23104 sequence
display id: BBa_J23104_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtattgtgctagc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z