BBa_K1537029 1 BBa_K1537029 HSP terminator 2014-09-17T11:00:00Z 2015-05-08T01:10:52Z Arabidopsis thaliana The heat shock protein ( HSP ) terminator increases mRNA levels of both transiently and stably expressed transgenes in plants effectively. false false _1919_ 0 22415 9 Not in stock false none. false Jie Li annotation2417608 1 HSP terminator range2417608 1 1 250 BBa_K1537028 1 BBa_K1537028 AHA2 2014-09-17T11:00:00Z 2015-05-08T01:10:52Z Arabidopsis thaliana AHA2 belongs to the P-type ATPase superfamily of cation-transporting ATPases, pumps protons out of the cell, generating a proton gradient that drives the active transport of nutrients by proton symport.We want to enlarge the tobacco AHA2 to false false _1919_ 0 22415 9 Not in stock true We have to remove some restriction endonuclease cutting sites by changing a few bases. false Jie Li annotation2417289 1 AHA2 range2417289 1 1 2847 BBa_K1537020 1 BBa_K1537020 GC1 promoter 2014-09-16T11:00:00Z 2015-05-08T01:10:52Z Arabidopsis. The GC1 promoter drives strong reporter expression in guard cells of Arabidopsis and tobacco plants. It provides a potent research tool for targeted guard cell expression. false false _1919_ 0 22415 9 It's complicated false no false Jie Li annotation2417291 1 GC1 promoter range2417291 1 1 1702 BBa_K1537031 1 BBa_K1537031 NOS ( nopaline synthase ) terminator 2014-09-17T11:00:00Z 2015-05-08T01:10:52Z Agrobacterium tumefasciens It???s a quite common terminator in expression system of plants. false false _1919_ 0 22415 9 Not in stock false None. false Jie Li annotation2417607 1 NOS terminator range2417607 1 1 253 BBa_K1537002 1 pIGEM002 it's our vector backbone. 2014-10-21T11:00:00Z 2015-05-08T01:10:52Z none. none. false false _1919_ 0 22415 9 Not in stock false none. false Jie Li component2428865 1 BBa_K1537019 component2428852 1 BBa_K1537016 component2428849 1 BBa_K1537015 component2428863 1 BBa_K1537020 component2428861 1 BBa_K1537028 component2428857 1 BBa_K1537029 component2428867 1 BBa_K1537027 component2428859 1 BBa_K1537031 component2428855 1 BBa_K1537017 component2428869 1 BBa_K1537030 annotation2428861 1 BBa_K1537028 range2428861 1 1531 4377 annotation2428852 1 BBa_K1537016 range2428852 1 869 934 annotation2428867 1 BBa_K1537027 range2428867 1 6966 8048 annotation2428849 1 BBa_K1537015 range2428849 1 1 860 annotation2428863 1 BBa_K1537020 range2428863 1 4386 6087 annotation2428865 1 BBa_K1537019 range2428865 1 6096 6959 annotation2428855 1 BBa_K1537017 range2428855 1 943 1005 annotation2428869 1 BBa_K1537030 range2428869 1 8057 8272 annotation2428857 1 BBa_K1537029 range2428857 1 1014 1263 annotation2428859 1 BBa_K1537031 range2428859 1 1272 1524 BBa_K1537030 1 BBa_K1537030 CaMV35S polyA 2014-09-17T11:00:00Z 2015-05-08T01:10:52Z Cauliflower mosaic virus It???s a kind of terminator derived from cauliflower mosaic virus. false false _1919_ 0 22415 9 Not in stock false None false Jie Li annotation2417606 1 CaMV35S polyA range2417606 1 1 216 BBa_K1537016 1 BBa_K1537016 GSG linker+P2A 2014-09-13T11:00:00Z 2015-05-08T01:10:52Z The part is from porcine teschovirus-1. The 18~22 amino acids 2A self-cleaving oligopeptides can be used for co-expression of multiple, discrete proteins from a single ORF.Based on highly inefficient peptide bond formation between glycine and proline residues within the 2A peptide, placement of 2A peptide sequence as a linker region between tandem cDNA???s allows the stoichiometric translation of multiple unfused protein products. These sequences were first discovered in the foot-and-mouth disease virus (FMDV).And since than many 2A-like sequences have been identified in other viruses and some parasites.To minimize the risk of homologous recombination, it is important to use different 2A peptide sequences if more than two genes are being linked. The 2A peptide system has thus far worked successfully in all eukaryotic systems tested, from mammalian cells, yeast, and plants.In our project,we use F2A(from foot-and-mouth disease virus), P2A(from porcine teschovirus-1) and T2A(from Thosea asigna virus) to achieve our goal.And "GSG linker" is an oligopeptide of ???Gly-Ser-Gly??? between your protein and 2A peptide to enhance cleavage. false false _1919_ 0 22415 9 In stock false We have to use this part by synthesis from biological company because the virus is dangerous. false Jie Li annotation2383427 1 P2A range2383427 1 10 66 annotation2383426 1 GSG linker range2383426 1 1 9 BBa_K1537019 1 BBa_K1537019 TA29 promoter 2014-09-15T11:00:00Z 2015-05-08T01:10:52Z tissue specific gene of tobacco. TA29 promoter is a tissue-specific(tapetal cells) promoter found in tobacco. false false _1919_ 0 22415 9 It's complicated false no false Jie Li annotation2417290 1 TA29 promoter range2417290 1 1 864 BBa_K1537017 1 BBa_K1537017 GSG linker+T2A 2014-09-15T11:00:00Z 2015-05-08T01:10:52Z T2A is from Thosea asigna virus. The 18~22 amino acids 2A self-cleaving oligopeptides can be used for co-expression of multiple, discrete proteins from a single ORF.Based on highly inefficient peptide bond formation between glycine and proline residues within the 2A peptide, placement of 2A peptide sequence as a linker region between tandem cDNA???s allows the stoichiometric translation of multiple unfused protein products. These sequences were first discovered in the foot-and-mouth disease virus (FMDV).And since than many 2A-like sequences have been identified in other viruses and some parasites.To minimize the risk of homologous recombination, it is important to use different 2A peptide sequences if more than two genes are being linked. The 2A peptide system has thus far worked successfully in all eukaryotic systems tested, from mammalian cells, yeast, and plants.In our project,we use F2A(from foot-and-mouth disease virus), P2A(from porcine teschovirus-1) and T2A(from Thosea asigna virus) to achieve our goal.And GSG linker is an oligopeptide of ???Gly-Ser-Gly??? between your protein and 2A peptide to enhance cleavage. false false _1919_ 0 22415 9 In stock false We have to use it by chemosynthesis because the virus is dangerous. false Jie Li annotation2383712 1 GSG linker range2383712 1 1 9 annotation2383711 1 T2A range2383711 1 10 63 BBa_K1537027 1 BBa_K1537027 AdCP 2014-09-16T11:00:00Z 2015-05-08T01:10:52Z Arachis diogoi ATGGCTAGGCTCTCTTTATTGTTGTTACTTCTCGTGGCAGCTGTGGCTACCGCAGTTGATGATCAGGCAGATCCTCTCATTAGGCAGGTTACTGATGGAGATCATCACATGCTTAATGCTGAACATCACTTCACTACATTCAAGACTAAGTTCGGAAAGTCTTATGCTACACAAGAAGAGCATGATTACAGATTTGGTGTTTTCAGGGCAAACCTCAGAAGGGCTAAACTTCATGCAAAACTTGATCCTAGTGCTGAGCATGGAGTTACAAAGTTTTCTGATCTTACCCCAGAAGAGTTCAAGAGACAATATCTCGGTTTAAAACCTCTTAGGTTGCCATCAACCGCTAATAAGGCACCTATTCTCCCTACTTCTGATCTTCCTGAAAACTTTGATTGGAGAGATAAGGGAGCTGTTACTCCAGTGAAAAATCAAGGAAGTTGTGGTTCTTGCTGGGCTTTCAGTACCACTGGTGCTCTTGAAGGTGCACATTACTTGTCAACAGGTGAACTCGTTTCTTTATCAGAGCAACAGTTGGTTGATTGTGATCACGTGTGCGATCCTGAAGAGTATGGAGCTTGTGATGCAGGTTGCAACGGAGGTCTTATGAATAACGCTTTCGATTACATTTTGCAAGCAGGAGGTGTGCAGACCGAAAAGGATTATCCATACTCTGGAAGAGATGAGACATGTAAATTTGATAAGTCAAAGGTTGCTGCAACCGTGGCTAACTTCTCAGTTGTGAGTCTCGATGAAGATCAAATCGCTGCAAATCTCGTTAAGCATGGACCTTTAGCTGTGGGAATTAACGCAATTTTCATGCAGACATACATCGGAGGTGTTTCTTGTCCATACATATGCGGAAAAAACTTGGATCACGGTGTTCTTTTGGTGGGATATGGTGCTGCTGGATACGCTCCTATTAGATTCAAGGATAAGCCATTCTGGATTATCAAAAATTCTTGGGGAGAATCATGGGGAGAGGATGGTTATTATAAGATTTGTAGAGGAAAGAATGTTTGTGGTGTGGATTCAATGGTTTCATCTGTGGTTGCTACTACTTTCACTTCATCAAATAACTGA false false _1919_ 0 22415 9 In stock true We have to remove some restriction endonuclease cutting sites by changing a few bases. false Jie Li annotation2417286 1 AdCP range2417286 1 1 1083 BBa_K1537015 1 BBa_K1537015 35S promoter+translation initiation optimized sequence for dicot+Mass translation enhancer 2014-08-17T11:00:00Z 2015-05-08T01:10:52Z The 35S promoter has a large area of application in genetically modified plants.The translation initiation optimized sequence for dicot is from the paper "translation initiation optimized sequence for dicot"(Nucleic Acids Research Volume 36, Issue 3Pp. 861-871 So Nakagawa1, Yoshihito Niimura2, Takashi Gojobori3,4, Hiroshi Tanaka1,2,* and Kin-ichiro Miura5) The 35S promoter is a strong promoter derived from cauliflower mosaic virus. This constitutive promoter is widely used in transgenic plants to improve the level of the expression of foreign genes effectively."AAAAAAATG" was a kind of preferred nucleotide sequence used in dicot plants（ATG is the initiation codon）to enhance translation initiation.And insertion of ???GCT TCC TCC???(mass translation enhancer) after the initiator codon ATG can augment downstream gene-expression in plants. false false _1919_ 0 22415 9 It's complicated false We have to remove some restriction endonuclease cutting sites by changing a few bases. false Jie Li annotation2393799 1 mass translation enhancer range2393799 1 852 860 annotation2393798 1 translation initiation optimized sequence for dicot range2393798 1 843 851 annotation2393797 1 35s promoter range2393797 1 1 842 BBa_K1537027_sequence 1 atggctaggctctctttattgttgttacttctcgtggcagctgtggctaccgcagttgatgatcaggcagatcctctcattaggcaggttactgatggagatcatcacatgcttaatgctgaacatcacttcactacattcaagactaagttcggaaagtcttatgctacacaagaagagcatgattacagatttggtgttttcagggcaaacctcagaagggctaaacttcatgcaaaacttgatcctagtgctgagcatggagttacaaagttttctgatcttaccccagaagagttcaagagacaatatctcggtttaaaacctcttaggttgccatcaaccgctaataaggcacctattctccctacttctgatcttcctgaaaactttgattggagagataagggagctgttactccagtgaaaaatcaaggaagttgtggttcttgctgggctttcagtaccactggtgctcttgaaggtgcacattacttgtcaacaggtgaactcgtttctttatcagagcaacagttggttgattgtgatcacgtgtgcgatcctgaagagtatggagcttgtgatgcaggttgcaacggaggtcttatgaataacgctttcgattacattttgcaagcaggaggtgtgcagaccgaaaaggattatccatactctggaagagatgagacatgtaaatttgataagtcaaaggttgctgcaaccgtggctaacttctcagttgtgagtctcgatgaagatcaaatcgctgcaaatctcgttaagcatggacctttagctgtgggaattaacgcaattttcatgcagacatacatcggaggtgtttcttgtccatacatatgcggaaaaaacttggatcacggtgttcttttggtgggatatggtgctgctggatacgctcctattagattcaaggataagccattctggattatcaaaaattcttggggagaatcatggggagaggatggttattataagatttgtagaggaaagaatgtttgtggtgtggattcaatggtttcatctgtggttgctactactttcacttcatcaaataactga BBa_K1537016_sequence 1 ggtagtggagcaacaaacttctcattattaaagcaagcaggagatgtggaggaaaatcctggtcca BBa_K1537019_sequence 1 tttttggttagcgaatgcatttaatttagacattgtgttatgttccacttaaccgcttccctgcacttctttcaatctatctctcgatagaaaattgtgatactttgcgacttctatcagaggactttttgttttccatgtaacaatctgtcattttcgatggggagatttgcacaaataggctatttatgtgtcccaatttaaattttaaccccatgtcgatcagaacttagccacgagcaccagaagtttgatggatatgtgactttgtcactatccggtttactaatcaagagctatttttattcaaaattggatatctagctaagtataactggataatttgcattaacagattgaatatagtgccaaacaagaagggacaattgacttgtcactttatgaaagatgattcaaacatgattttttatgtactaatatatacatcctactcgaattaaagcgacataggctcgaagtatgcacatttagcaatgtaaattaaatcagtttttgaatcaagctaaaagcagacttgcataaggtgggtggctggactagaataaacatcttctctagcacagcttcataatgtaatttccataactgaaatcagggtgagacaaaattttggtactttttcctcacactaagtccatgtttgcaacaatttaatacatgaaaccttaatgttaccctcagattagcctgctactccccattttcctcgaaatgctccaacaaaagttagttttgcaagttgttgtgtatgtcttgtgctctatatatgcccttgtggtgcaagtgtaacagtacaacatcatcactcaaatcaaagtttttacttaaagaaattagctaaaa BBa_K1537031_sequence 1 cgttcaaacatttggcaataaagtttcttaagattgaatcctgttgccggtcttgcgatgattatcatataatttctgttgaattacgttaagcatgtaataattaacatgtaatgcatgacgttatttatgagatgggtttttatgattagagtcccgcaattatacatttaatacgcgatagaaaacaaaatatagcgcgcaaactaggataaattatcgcgcgcggtgtcatctatgttactagatcggg BBa_K1537017_sequence 1 ggtagtggagagggtagaggatctcttttgacatgtggagatgttgaagagaaccctggacca BBa_K1537020_sequence 1 taacccgatgctcctgctcttcctcaagaccttccttgattcgccgccggtatgttctccgtctgtggtagcgcctttggaacactctaccaacgccgccatgaaaggatctctcatggccgcaggggacgtgttcttcttacatctggtgttagggctatggttactccagtgaggagggagaggcaagaggttgcttaatgattcgtttttccggtgatacgagaactctttaggtttaccgggaaggttttcccatgaaaatgggatgccaagtggatggagaggagttgccggagagttgccggagaataggagggaattggaggaggaggaagagagtgatcgccgggttgaaatcttaaccgtcgaggagaatttgaccgagttggatcgtctagtaggtacaattcgggtccttggcgaagtatccattcaaaatagtgtttagttttggacttgagaacttgttgtctctttgatctcttttatataaaactttggacgtgtaggacaaacttgtcaacataagaaacaaaatggttgcaacagagaggatgaatttataagttttcaacaccgcttttcttattagacggacaacaatctatagtggagtaaatttttatttttggtaaaatggttagtcaattcaaatatctaaattttgtgactcactaacattaacaaatatgcataagacataaaaaaaagaaagaataattcttatgaaacaagaaaaaaaacctatacaatcaatctttaggaattgacgatgtagaattgtagatgataaattttctcaaatatagatgggcctaatgaagggtgccgcttattggatctgacccattttgaggacaataatattttcattggttataagccttttaatcaaaattgtcattaaattgatgtctccctctcgggtcattttcctttctccctcacaaataatgtagactttagcaatttgctcgctgtgctttgtctttatatttagtaacacaaacattttgacttgtcttgtagagtttttctcttttatttttctatccaatatgaaaactaaaagtgttctcgtatacatatattaaaattaaagaaacctatgaaaacaccaatacaaatgcgatattgttttcagttcgacgtttcatgtttgttagaaaatttctaatgacgtttgtataaaatagacaattaaacgccaaacactacatctgtgttttcgaacaatattgcgtctgcgtttccttcatctatctctctcagtgtcacaatgtctgaactaagagacagctgtaaactatcattaagacataaactaccaaagtatcaagctaatgtaaaaattactctcatttccacgtaacaaattgagttagcttaacatattagtgaaactaggtttgaattttcttcttcttcttccatgcatcctccgaaaaaagggaaccaatcaaaactgtttgcatatcaaactccaacactttacagcaaatgcaatctataatctgtgatttatccaataaaaacctgtgatttatgtttggctccagcgatgaaagtctatgcatgtgatctctatccaacatgagtaattgttcagaaaataaaaagtagctgaaatgtatctatataaagaatcatccacaagtactattttcacacactacttcaaaatcactactcaagaaat BBa_K1537029_sequence 1 atatgaagatgaagatgaaatatttggtgtgtcaaataaaaaccttgtgtggttaagtttgtgtttttttcttggcttgttgtgttatgaatttgtggctttttctaatattaaatgaatgtaagatcacattataatgaataaacaaatgtttctataatccattgtgaatgttttgttggatctcttctgcaccatataactactgtatgtgctatggtatggactatggaatatgattaaagataag BBa_K1537030_sequence 1 tcgacaagctctattttctccataataatgtgtgagtagttcccagataagggaattagggttcctatagggtttcgctcatgtgttgagcatataagaaacccttagtatgtatttgtatttgtaaaatacttctatcaataaaatttctaattcctaaaaccaaaatccagtactaaaatccagatcccccgaattaaatcggcgttaattcag BBa_K1537015_sequence 1 agatttgccttttcaatttcagaaagaatgctaacccacagatggttagagaggcttacgcagcaggtctcatcaagacgatctacccgagcaataatctccaggaaatcaaataccttcccaagaaggttaaagatgcagtcaaaagattcaggactaactgcatcaagaacacagagaaagatatatttctcaagatcagaagtactattccagtatggacgattcaaggcttgcttcacaaaccaaggcaagtaatagagattggagtctctaaaaaggtagttcccactgaatcaaaggccatggagtcaaagattcaaatagaggacctaacagaactcgccgtaaagactggcgaacagttcatacagagtctcttacgactcaatgacaagaagaaaatcttcgtcaacatggtggagcacgacacacttgtctactccaaaaatatcaaagatacagtctcagaagaccaaagggcaattgagacttttcaacaaagggtaatatccggaaacctcctcggattccattgcccagctatctgtcactttattgtgaagatagtggaaaaggaaggtggctcctacaaatgccatcattgcgataaaggaaaggccatcgttgaagatgcctctgccgacagtggtcccaaagatggacccccacccacgaggagcatcgtggaaaaagaagacgttccaaccacgtcttcaaagcaagtggattgatgtgatatctccactgacgtaagggatgacgcacaatcccactatccttcgcaagacccttcctctatataaggaagttcatttcatttggagagaacacgggggactaaaaaaatggcttcctcc BBa_K1537028_sequence 1 atgtcatcattagaagatattaagaacgagactgtggatttggagaagatacctatagaggaagtgtttcagcaactcaagtgtagtagagagggtttgactacacaagaaggagaggataggatacagatttttggtccaaacaaacttgaagagaagaaagagtctaaacttttgaaatttcttggattcatgtggaatcctttgagttgggttatggaaatggctgcaatcatggctatagcacttgctaacggagatggtagacctccagattggcaagattttgtgggtattatctgtctcttagttatcaattcaactattagtttcatcgaagagaataacgctggaaacgctgctgctgctcttatggctggtcttgcaccaaagacaaaagttcttagagatggaaagtggtctgaacaagaggctgcaattctcgttcctggagatattgtgtcaattaaattaggagatattattccagcagatgctagacttttggagggagatcctttgaaggttgatcagtctgctcttacaggagagtcattgccagttaccaagcatcctggtcaagaagtgttcagtggatctacttgcaaacagggtgaaattgaggctgttgtgatcgcaaccggagttcatactttctttggaaaggctgcacacctcgttgattcaacaaatcaagtgggacactttcagaaagttcttaccgcaatcggtaacttctgtatttgctctatcgctattggaatggttattgaaatcatagtgatgtatccaatccaaagaaggaaatacagagatggtatagataacctcttagttcttttgattggaggtattccaatcgcaatgcctactgttttgtctgtgacaatggctattggaagtcatagactctctcaacagggtgctataacaaaaaggatgaccgcaattgaagagatggctggtatggatgttctttgttctgataagaccggaactctcacactcaacaaactttcagtggataagaacttggttgaggtgttttgcaagggagttgaaaaagatcaagtgctcttattcgctgcaatggcttcaagagttgaaaatcaggatgctatcgatgctgcaatggtgggaatgttggctgatccaaaagaggcaagagctggaattagggaggttcattttcttccattcaatcctgtggataagagaaccgctttgacttatatcgatggaagtggtaactggcacagggtttctaagggtgctcctgagcaaattcttgaattggcaaaggcttctaacgatctcagtaagaaagttttaagtattatcgataagtacgctgagagaggactcaggtctttagcagtggctagacaggttgtgccagagaagactaaagaatctcctggtgctccttgggagtttgttggtcttttgcctcttttcgatcctccaaggcatgattctgctgaaactatcagaagggctctcaatcttggagttaatgtgaagatgatcacaggagatcaacttgctatcggtaaagaaaccggaagaaggttgggaatgggtactaatatgtatccttcttcagctctcttaggtactcacaaggatgctaacctcgcatctattccagttgaagagttaatcgagaaagctgatggttttgcaggagttttccctgagcataagtacgaaatcgtgaagaaacttcaggaaagaaagcatattgttggtatgacaggagatggtgttaatgatgcaccagctttgaagaaagctgatataggaattgcagttgctgatgcaactgatgctgcaagaggtgcttctgatattgtgcttacagagcctggattgtctgttataatttcagcagtgttgacaagtagagctatcttccaaaggatgaaaaactacactatctatgctgtgtctatcacaataagaattgttttcggtttcatgcttatcgctttgatatgggagtttgatttctcagcattcatggttctcatcatagctatcttaaacgatggaacaattatgaccatctctaaggatagagttaaacctagtccaacccctgattcttggaagctcaaagaaatttttgcaactggagttgtgcttggaggttatcaggctattatgacagttatctttttctgggctgcacataagaccgatttcttttctgatactttcggtgttagaagtatcagggataataaccacgaactcatgggagctgtttacttacaagtgtctattatctcacaggctcttatttttgttactagatcaaggagttggtctttcgtggaaagacctggtgctcttttgatgatagcttttcttattgcacaactcatcgctactcttattgctgtttacgcaaactgggaatttgcaaagataagaggaattggttggggatgggctggagtgatctggctttactcaatagttacttacttccctttggatgtgtttaagttcgcaatcaggtatatattgtctggaaaggcttggcttaatttgttcgagaacaaaactgcttttactatgaagaaggattacggaaaggaagagagagaagctcaatgggctcttgcacagaggactctccatggtttacaaccaaaggaggctgttaatattttccctgaaaagggatcatacagagaattgagtgagatcgctgaacaggcaaagagaagggctgagattgcaagacttagggaattgcatacactcaaaggacacgtggagtcagttgttaagttgaagggtttagatatagagacaccaagtcactacacagtttga BBa_K1537002_sequence 1 agatttgccttttcaatttcagaaagaatgctaacccacagatggttagagaggcttacgcagcaggtctcatcaagacgatctacccgagcaataatctccaggaaatcaaataccttcccaagaaggttaaagatgcagtcaaaagattcaggactaactgcatcaagaacacagagaaagatatatttctcaagatcagaagtactattccagtatggacgattcaaggcttgcttcacaaaccaaggcaagtaatagagattggagtctctaaaaaggtagttcccactgaatcaaaggccatggagtcaaagattcaaatagaggacctaacagaactcgccgtaaagactggcgaacagttcatacagagtctcttacgactcaatgacaagaagaaaatcttcgtcaacatggtggagcacgacacacttgtctactccaaaaatatcaaagatacagtctcagaagaccaaagggcaattgagacttttcaacaaagggtaatatccggaaacctcctcggattccattgcccagctatctgtcactttattgtgaagatagtggaaaaggaaggtggctcctacaaatgccatcattgcgataaaggaaaggccatcgttgaagatgcctctgccgacagtggtcccaaagatggacccccacccacgaggagcatcgtggaaaaagaagacgttccaaccacgtcttcaaagcaagtggattgatgtgatatctccactgacgtaagggatgacgcacaatcccactatccttcgcaagacccttcctctatataaggaagttcatttcatttggagagaacacgggggactaaaaaaatggcttcctcctactagagggtagtggagcaacaaacttctcattattaaagcaagcaggagatgtggaggaaaatcctggtccatactagagggtagtggagagggtagaggatctcttttgacatgtggagatgttgaagagaaccctggaccatactagagatatgaagatgaagatgaaatatttggtgtgtcaaataaaaaccttgtgtggttaagtttgtgtttttttcttggcttgttgtgttatgaatttgtggctttttctaatattaaatgaatgtaagatcacattataatgaataaacaaatgtttctataatccattgtgaatgttttgttggatctcttctgcaccatataactactgtatgtgctatggtatggactatggaatatgattaaagataagtactagagcgttcaaacatttggcaataaagtttcttaagattgaatcctgttgccggtcttgcgatgattatcatataatttctgttgaattacgttaagcatgtaataattaacatgtaatgcatgacgttatttatgagatgggtttttatgattagagtcccgcaattatacatttaatacgcgatagaaaacaaaatatagcgcgcaaactaggataaattatcgcgcgcggtgtcatctatgttactagatcgggtactagatgtcatcattagaagatattaagaacgagactgtggatttggagaagatacctatagaggaagtgtttcagcaactcaagtgtagtagagagggtttgactacacaagaaggagaggataggatacagatttttggtccaaacaaacttgaagagaagaaagagtctaaacttttgaaatttcttggattcatgtggaatcctttgagttgggttatggaaatggctgcaatcatggctatagcacttgctaacggagatggtagacctccagattggcaagattttgtgggtattatctgtctcttagttatcaattcaactattagtttcatcgaagagaataacgctggaaacgctgctgctgctcttatggctggtcttgcaccaaagacaaaagttcttagagatggaaagtggtctgaacaagaggctgcaattctcgttcctggagatattgtgtcaattaaattaggagatattattccagcagatgctagacttttggagggagatcctttgaaggttgatcagtctgctcttacaggagagtcattgccagttaccaagcatcctggtcaagaagtgttcagtggatctacttgcaaacagggtgaaattgaggctgttgtgatcgcaaccggagttcatactttctttggaaaggctgcacacctcgttgattcaacaaatcaagtgggacactttcagaaagttcttaccgcaatcggtaacttctgtatttgctctatcgctattggaatggttattgaaatcatagtgatgtatccaatccaaagaaggaaatacagagatggtatagataacctcttagttcttttgattggaggtattccaatcgcaatgcctactgttttgtctgtgacaatggctattggaagtcatagactctctcaacagggtgctataacaaaaaggatgaccgcaattgaagagatggctggtatggatgttctttgttctgataagaccggaactctcacactcaacaaactttcagtggataagaacttggttgaggtgttttgcaagggagttgaaaaagatcaagtgctcttattcgctgcaatggcttcaagagttgaaaatcaggatgctatcgatgctgcaatggtgggaatgttggctgatccaaaagaggcaagagctggaattagggaggttcattttcttccattcaatcctgtggataagagaaccgctttgacttatatcgatggaagtggtaactggcacagggtttctaagggtgctcctgagcaaattcttgaattggcaaaggcttctaacgatctcagtaagaaagttttaagtattatcgataagtacgctgagagaggactcaggtctttagcagtggctagacaggttgtgccagagaagactaaagaatctcctggtgctccttgggagtttgttggtcttttgcctcttttcgatcctccaaggcatgattctgctgaaactatcagaagggctctcaatcttggagttaatgtgaagatgatcacaggagatcaacttgctatcggtaaagaaaccggaagaaggttgggaatgggtactaatatgtatccttcttcagctctcttaggtactcacaaggatgctaacctcgcatctattccagttgaagagttaatcgagaaagctgatggttttgcaggagttttccctgagcataagtacgaaatcgtgaagaaacttcaggaaagaaagcatattgttggtatgacaggagatggtgttaatgatgcaccagctttgaagaaagctgatataggaattgcagttgctgatgcaactgatgctgcaagaggtgcttctgatattgtgcttacagagcctggattgtctgttataatttcagcagtgttgacaagtagagctatcttccaaaggatgaaaaactacactatctatgctgtgtctatcacaataagaattgttttcggtttcatgcttatcgctttgatatgggagtttgatttctcagcattcatggttctcatcatagctatcttaaacgatggaacaattatgaccatctctaaggatagagttaaacctagtccaacccctgattcttggaagctcaaagaaatttttgcaactggagttgtgcttggaggttatcaggctattatgacagttatctttttctgggctgcacataagaccgatttcttttctgatactttcggtgttagaagtatcagggataataaccacgaactcatgggagctgtttacttacaagtgtctattatctcacaggctcttatttttgttactagatcaaggagttggtctttcgtggaaagacctggtgctcttttgatgatagcttttcttattgcacaactcatcgctactcttattgctgtttacgcaaactgggaatttgcaaagataagaggaattggttggggatgggctggagtgatctggctttactcaatagttacttacttccctttggatgtgtttaagttcgcaatcaggtatatattgtctggaaaggcttggcttaatttgttcgagaacaaaactgcttttactatgaagaaggattacggaaaggaagagagagaagctcaatgggctcttgcacagaggactctccatggtttacaaccaaaggaggctgttaatattttccctgaaaagggatcatacagagaattgagtgagatcgctgaacaggcaaagagaagggctgagattgcaagacttagggaattgcatacactcaaaggacacgtggagtcagttgttaagttgaagggtttagatatagagacaccaagtcactacacagtttgatactagagtaacccgatgctcctgctcttcctcaagaccttccttgattcgccgccggtatgttctccgtctgtggtagcgcctttggaacactctaccaacgccgccatgaaaggatctctcatggccgcaggggacgtgttcttcttacatctggtgttagggctatggttactccagtgaggagggagaggcaagaggttgcttaatgattcgtttttccggtgatacgagaactctttaggtttaccgggaaggttttcccatgaaaatgggatgccaagtggatggagaggagttgccggagagttgccggagaataggagggaattggaggaggaggaagagagtgatcgccgggttgaaatcttaaccgtcgaggagaatttgaccgagttggatcgtctagtaggtacaattcgggtccttggcgaagtatccattcaaaatagtgtttagttttggacttgagaacttgttgtctctttgatctcttttatataaaactttggacgtgtaggacaaacttgtcaacataagaaacaaaatggttgcaacagagaggatgaatttataagttttcaacaccgcttttcttattagacggacaacaatctatagtggagtaaatttttatttttggtaaaatggttagtcaattcaaatatctaaattttgtgactcactaacattaacaaatatgcataagacataaaaaaaagaaagaataattcttatgaaacaagaaaaaaaacctatacaatcaatctttaggaattgacgatgtagaattgtagatgataaattttctcaaatatagatgggcctaatgaagggtgccgcttattggatctgacccattttgaggacaataatattttcattggttataagccttttaatcaaaattgtcattaaattgatgtctccctctcgggtcattttcctttctccctcacaaataatgtagactttagcaatttgctcgctgtgctttgtctttatatttagtaacacaaacattttgacttgtcttgtagagtttttctcttttatttttctatccaatatgaaaactaaaagtgttctcgtatacatatattaaaattaaagaaacctatgaaaacaccaatacaaatgcgatattgttttcagttcgacgtttcatgtttgttagaaaatttctaatgacgtttgtataaaatagacaattaaacgccaaacactacatctgtgttttcgaacaatattgcgtctgcgtttccttcatctatctctctcagtgtcacaatgtctgaactaagagacagctgtaaactatcattaagacataaactaccaaagtatcaagctaatgtaaaaattactctcatttccacgtaacaaattgagttagcttaacatattagtgaaactaggtttgaattttcttcttcttcttccatgcatcctccgaaaaaagggaaccaatcaaaactgtttgcatatcaaactccaacactttacagcaaatgcaatctataatctgtgatttatccaataaaaacctgtgatttatgtttggctccagcgatgaaagtctatgcatgtgatctctatccaacatgagtaattgttcagaaaataaaaagtagctgaaatgtatctatataaagaatcatccacaagtactattttcacacactacttcaaaatcactactcaagaaattactagagtttttggttagcgaatgcatttaatttagacattgtgttatgttccacttaaccgcttccctgcacttctttcaatctatctctcgatagaaaattgtgatactttgcgacttctatcagaggactttttgttttccatgtaacaatctgtcattttcgatggggagatttgcacaaataggctatttatgtgtcccaatttaaattttaaccccatgtcgatcagaacttagccacgagcaccagaagtttgatggatatgtgactttgtcactatccggtttactaatcaagagctatttttattcaaaattggatatctagctaagtataactggataatttgcattaacagattgaatatagtgccaaacaagaagggacaattgacttgtcactttatgaaagatgattcaaacatgattttttatgtactaatatatacatcctactcgaattaaagcgacataggctcgaagtatgcacatttagcaatgtaaattaaatcagtttttgaatcaagctaaaagcagacttgcataaggtgggtggctggactagaataaacatcttctctagcacagcttcataatgtaatttccataactgaaatcagggtgagacaaaattttggtactttttcctcacactaagtccatgtttgcaacaatttaatacatgaaaccttaatgttaccctcagattagcctgctactccccattttcctcgaaatgctccaacaaaagttagttttgcaagttgttgtgtatgtcttgtgctctatatatgcccttgtggtgcaagtgtaacagtacaacatcatcactcaaatcaaagtttttacttaaagaaattagctaaaatactagatggctaggctctctttattgttgttacttctcgtggcagctgtggctaccgcagttgatgatcaggcagatcctctcattaggcaggttactgatggagatcatcacatgcttaatgctgaacatcacttcactacattcaagactaagttcggaaagtcttatgctacacaagaagagcatgattacagatttggtgttttcagggcaaacctcagaagggctaaacttcatgcaaaacttgatcctagtgctgagcatggagttacaaagttttctgatcttaccccagaagagttcaagagacaatatctcggtttaaaacctcttaggttgccatcaaccgctaataaggcacctattctccctacttctgatcttcctgaaaactttgattggagagataagggagctgttactccagtgaaaaatcaaggaagttgtggttcttgctgggctttcagtaccactggtgctcttgaaggtgcacattacttgtcaacaggtgaactcgtttctttatcagagcaacagttggttgattgtgatcacgtgtgcgatcctgaagagtatggagcttgtgatgcaggttgcaacggaggtcttatgaataacgctttcgattacattttgcaagcaggaggtgtgcagaccgaaaaggattatccatactctggaagagatgagacatgtaaatttgataagtcaaaggttgctgcaaccgtggctaacttctcagttgtgagtctcgatgaagatcaaatcgctgcaaatctcgttaagcatggacctttagctgtgggaattaacgcaattttcatgcagacatacatcggaggtgtttcttgtccatacatatgcggaaaaaacttggatcacggtgttcttttggtgggatatggtgctgctggatacgctcctattagattcaaggataagccattctggattatcaaaaattcttggggagaatcatggggagaggatggttattataagatttgtagaggaaagaatgtttgtggtgtggattcaatggtttcatctgtggttgctactactttcacttcatcaaataactgatactagagtcgacaagctctattttctccataataatgtgtgagtagttcccagataagggaattagggttcctatagggtttcgctcatgtgttgagcatataagaaacccttagtatgtatttgtatttgtaaaatacttctatcaataaaatttctaattcctaaaaccaaaatccagtactaaaatccagatcccccgaattaaatcggcgttaattcag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z