LOCUS BBa_K1537009 11707 bp DNA linear 22-Dec-2024 DEFINITION piGEM002+HPS-PHI+AtFDH+FALDH ACCESSION BBa_K1537009 VERSION BBa_K1537009.1 FEATURES Location/Qualifiers promoter 1..842 /label=35s promoter misc_feature 843..851 /label=translation initiation optimized sequence for dicot misc_feature 852..860 /label=mass translation enhancer promoter 1..860 /label=BBa_K1537015 CDS 867..1487 /label=HPS CDS 1488..2087 /label=PHI misc_feature 867..2087 /label=BBa_K1537024 misc_feature 2096..2104 /label=GSG linker misc_feature 2105..2161 /label=P2A promoter 2096..2161 /label=BBa_K1537016 CDS 2168..3232 /label=FDH CDS 2168..3232 /label=BBa_K1537025 misc_feature 3250..3303 /label=T2A misc_feature 3241..3249 /label=GSG linker promoter 3241..3303 /label=BBa_K1537017 CDS 3310..4440 /label=FALDH CDS 3310..4440 /label=BBa_K1537026 misc_feature 4449..4698 /label=HSP terminator terminator 4449..4698 /label=BBa_K1537029 misc_feature 4707..4959 /label=NOS terminator terminator 4707..4959 /label=BBa_K1537031 CDS 4966..7812 /label=AHA2 CDS 4966..7812 /label=BBa_K1537028 promoter 7821..9522 /label=GC1 promoter promoter 7821..9522 /label=BBa_K1537020 promoter 9531..10394 /label=TA29 promoter promoter 9531..10394 /label=BBa_K1537019 misc_feature 10401..11483 /label=AdCP CDS 10401..11483 /label=BBa_K1537027 misc_feature 11492..11707 /label=CaMV35S polyA terminator 11492..11707 /label=BBa_K1537030 ORIGIN 1 agatttgcct tttcaatttc agaaagaatg ctaacccaca gatggttaga gaggcttacg 61 cagcaggtct catcaagacg atctacccga gcaataatct ccaggaaatc aaataccttc 121 ccaagaaggt taaagatgca gtcaaaagat tcaggactaa ctgcatcaag aacacagaga 181 aagatatatt tctcaagatc agaagtacta ttccagtatg gacgattcaa ggcttgcttc 241 acaaaccaag gcaagtaata gagattggag tctctaaaaa ggtagttccc actgaatcaa 301 aggccatgga gtcaaagatt caaatagagg acctaacaga actcgccgta aagactggcg 361 aacagttcat acagagtctc ttacgactca atgacaagaa gaaaatcttc gtcaacatgg 421 tggagcacga cacacttgtc tactccaaaa atatcaaaga tacagtctca gaagaccaaa 481 gggcaattga gacttttcaa caaagggtaa tatccggaaa cctcctcgga ttccattgcc 541 cagctatctg tcactttatt gtgaagatag tggaaaagga aggtggctcc tacaaatgcc 601 atcattgcga taaaggaaag gccatcgttg aagatgcctc tgccgacagt ggtcccaaag 661 atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa 721 agcaagtgga ttgatgtgat atctccactg acgtaaggga tgacgcacaa tcccactatc 781 cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagaga acacggggga 841 ctaaaaaaat ggcttcctcc tactagatga aacttcaggt tgctatcgat cttttgtcaa 901 cagaagctgc attagagctt gctggaaagg tggcagaata tgttgatatt atcgagttag 961 gtactcctct tatagaagct gagggattgt cagtgattac agctgttaag aaagcacatc 1021 cagataagat cgttttcgct gatatgaaga ctatggatgc aggtgaactt gaggctgata 1081 tagcattcaa agctggtgca gatttggtga cagttctcgg aagtgctgat gattctacca 1141 ttgctggagc agttaaggct gcacaagctc ataataaggg tgttgtggtt gatttgattg 1201 gtatagaaga taaggctaca agagcacaag aggtgagggc tctcggtgca aaatttgttg 1261 aaatgcacgc tggattagat gagcaggcaa agcctggatt cgatcttaat ggtcttcttg 1321 ctgctggaga aaaggctaga gtgcctttct ctgttgcagg aggtgtgaag gttgctacaa 1381 ttccagcagt tcaaaaagct ggtgcagagg tggctgttgc aggaggtgct atctacggag 1441 ctgctgatcc tgctgctgct gctaaagaat tgagagctgc aatagctatg actcaggctg 1501 ctgaagctga tggtgcagtt aaggtggttg gagatgatat taccaataac ttgtcactcg 1561 tgagggatga agttgctgat actgctgcaa aagttgatcc tgagcaggtg gctgttcttg 1621 caagacaaat cgttcagcct ggtagggtgt ttgttgctgg agcaggtaga tcaggattag 1681 ttcttagaat ggctgcaatg aggttaatgc atttcggtct taccgtgcac gttgctggag 1741 atactacaac ccctgctata tctgctggag atttgttgct cgttgcttct ggatcaggta 1801 ctacatcagg agtggttaaa agtgctgaaa ctgcaaagaa agctggagca agaattgctg 1861 cttttactac taatcctgat tctccattgg ctggactcgc tgatgcagtg gttataattc 1921 cagctgcaca aaagactgat catggttctc acatttcaag gcaatatgct ggaagtttgt 1981 ttgaacaggt gctcttcgtg gttacagagg ctgttttcca gtctttatgg gatcacacag 2041 aggtggaggc tgaagaatta tggacaagac acgcaaactt agagtgatac tagagggtag 2101 tggagcaaca aacttctcat tattaaagca agcaggagat gtggaggaaa atcctggtcc 2161 atactagatg tcttcaggag atagtaagaa aattgttggt gtgttttata aggctaacga 2221 gtacgcaact aaaaatccaa acttccttgg atgtgttgag aatgctcttg gtattagaga 2281 ttggttggaa tcacaaggac atcagtatat cgttacagat gataaggagg gtccagattg 2341 cgaacttgag aaacatattc ctgatctcca cgttttaatc tctacaccat ttcatcctgc 2401 ttacgtgacc gcagaaagga ttaagaaagc taagaacttg aaacttttgc tcaccgcagg 2461 aatcggttca gatcacattg atcttcaagc tgctgctgct gctggactta ctgttgctga 2521 agtgactggt agtaacgttg tgtctgttgc agaagatgag ttgatgagaa ttttgattct 2581 tatgaggaac ttcgttcctg gatataacca agttgtgaag ggagagtgga atgtggcagg 2641 tatcgcttat agagcatacg atcttgaagg aaaaaccata ggtactgttg gagctggtag 2701 aattggaaag ttacttttgc agaggttgaa accatttggt tgtaatctct tataccatga 2761 taggctccaa atggctcctg aattagagaa ggaaacaggt gcaaaattcg ttgaggatct 2821 taatgagatg ttgccaaagt gcgatgttat agtgattaac atgcctctca cagagaaaac 2881 cagaggaatg ttcaataagg aactcatagg aaaattaaag aaaggtgttt tgatcgtgaa 2941 taacgctaga ggagctatta tggaaaggca ggctgttgtg gatgcagttg aatcaggaca 3001 catcggaggt tatagtggag atgtttggga ccctcaacca gctcctaagg atcatccatg 3061 gagatacatg cctaaccagg ctatgactcc acacacatct ggaactacaa tcgatgctca 3121 actcagatat gcagctggaa ctaaggatat gttagagagg tacttcaaag gtgaagattt 3181 tccaaccgag aattacatag tgaaggatgg tgaacttgct ccacaataca ggtactagag 3241 ggtagtggag agggtagagg atctcttttg acatgtggag atgttgaaga gaaccctgga 3301 ccatactaga tgggagcagt gacttaccaa ggaaaaaatt ctatcgctgt taagaaagtg 3361 gatgcaccat caattcagga tagagaggat gttattatca ggatcacttc tacagctata 3421 tgtggatctg atcttcatct ttatcagggt aacttccctt tgccaatagg atacgttatt 3481 ggtcacgaac ctatgggaat tgttgaagaa gtgggtccag atgttacagc tgtgaagaaa 3541 ggagatagag ttgtgatccc ttttaccgtt gcatgtggta aatgccaata ttgtgatcat 3601 catcttgaat cacagtgcga taatagtaac ccacattatg atagtggagg tttattcgga 3661 tactctgaga agtatggtaa ttaccctgga ggtcaagctg aatatctcag ggttccattt 3721 ggaaactaca cacctttcaa aattccagat gattgcgaat tggaggatga acagcttttg 3781 tttctctctg atgtgttacc taccgcttac tggtctgttg agcatgcagg agtgaagaaa 3841 ggagatacag ttatcgtgct cggatgtggt ccagttggac ttatggctca acagttcgca 3901 tggcaaaagg gtgctgaaag agttattgca gtggattata tcgattacag acttaggcac 3961 gctaaaagga tgaatggtgt tgaggtgttt gatttcactg aagatcctga tatgggtgag 4021 acacttaaag aattgaccaa aggaggtgca gatgttgtga ttgattgtgt tggtatggat 4081 ggaaagaagt ctcctttgga gaagatcgaa caaaaactta aacttcaagg aggtactata 4141 ggtccaatcc aaatagctac aaaggcagtt agaaaaaggg gaaccgttca gatgactggt 4201 gtgtatggag gtctttacaa tatgtttcct cttggagctt tctttgcaag aaacgttaca 4261 cttaaaatgg gacaagctcc agcaaggggt tatatgtcaa agttgtacca gaaagtgacc 4321 agtggagaga ttgatcctag agctataatt actcacaaac tcccattaga tgaagctgca 4381 cacgcatacc atatcttcaa cgataaacag gatgattgca taaaagtgat tctcaagcct 4441 tactagagat atgaagatga agatgaaata tttggtgtgt caaataaaaa ccttgtgtgg 4501 ttaagtttgt gtttttttct tggcttgttg tgttatgaat ttgtggcttt ttctaatatt 4561 aaatgaatgt aagatcacat tataatgaat aaacaaatgt ttctataatc cattgtgaat 4621 gttttgttgg atctcttctg caccatataa ctactgtatg tgctatggta tggactatgg 4681 aatatgatta aagataagta ctagagcgtt caaacatttg gcaataaagt ttcttaagat 4741 tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc 4801 atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag 4861 tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata 4921 aattatcgcg cgcggtgtca tctatgttac tagatcgggt actagatgtc atcattagaa 4981 gatattaaga acgagactgt ggatttggag aagataccta tagaggaagt gtttcagcaa 5041 ctcaagtgta gtagagaggg tttgactaca caagaaggag aggataggat acagattttt 5101 ggtccaaaca aacttgaaga gaagaaagag tctaaacttt tgaaatttct tggattcatg 5161 tggaatcctt tgagttgggt tatggaaatg gctgcaatca tggctatagc acttgctaac 5221 ggagatggta gacctccaga ttggcaagat tttgtgggta ttatctgtct cttagttatc 5281 aattcaacta ttagtttcat cgaagagaat aacgctggaa acgctgctgc tgctcttatg 5341 gctggtcttg caccaaagac aaaagttctt agagatggaa agtggtctga acaagaggct 5401 gcaattctcg ttcctggaga tattgtgtca attaaattag gagatattat tccagcagat 5461 gctagacttt tggagggaga tcctttgaag gttgatcagt ctgctcttac aggagagtca 5521 ttgccagtta ccaagcatcc tggtcaagaa gtgttcagtg gatctacttg caaacagggt 5581 gaaattgagg ctgttgtgat cgcaaccgga gttcatactt tctttggaaa ggctgcacac 5641 ctcgttgatt caacaaatca agtgggacac tttcagaaag ttcttaccgc aatcggtaac 5701 ttctgtattt gctctatcgc tattggaatg gttattgaaa tcatagtgat gtatccaatc 5761 caaagaagga aatacagaga tggtatagat aacctcttag ttcttttgat tggaggtatt 5821 ccaatcgcaa tgcctactgt tttgtctgtg acaatggcta ttggaagtca tagactctct 5881 caacagggtg ctataacaaa aaggatgacc gcaattgaag agatggctgg tatggatgtt 5941 ctttgttctg ataagaccgg aactctcaca ctcaacaaac tttcagtgga taagaacttg 6001 gttgaggtgt tttgcaaggg agttgaaaaa gatcaagtgc tcttattcgc tgcaatggct 6061 tcaagagttg aaaatcagga tgctatcgat gctgcaatgg tgggaatgtt ggctgatcca 6121 aaagaggcaa gagctggaat tagggaggtt cattttcttc cattcaatcc tgtggataag 6181 agaaccgctt tgacttatat cgatggaagt ggtaactggc acagggtttc taagggtgct 6241 cctgagcaaa ttcttgaatt ggcaaaggct tctaacgatc tcagtaagaa agttttaagt 6301 attatcgata agtacgctga gagaggactc aggtctttag cagtggctag acaggttgtg 6361 ccagagaaga ctaaagaatc tcctggtgct ccttgggagt ttgttggtct tttgcctctt 6421 ttcgatcctc caaggcatga ttctgctgaa actatcagaa gggctctcaa tcttggagtt 6481 aatgtgaaga tgatcacagg agatcaactt gctatcggta aagaaaccgg aagaaggttg 6541 ggaatgggta ctaatatgta tccttcttca gctctcttag gtactcacaa ggatgctaac 6601 ctcgcatcta ttccagttga agagttaatc gagaaagctg atggttttgc aggagttttc 6661 cctgagcata agtacgaaat cgtgaagaaa cttcaggaaa gaaagcatat tgttggtatg 6721 acaggagatg gtgttaatga tgcaccagct ttgaagaaag ctgatatagg aattgcagtt 6781 gctgatgcaa ctgatgctgc aagaggtgct tctgatattg tgcttacaga gcctggattg 6841 tctgttataa tttcagcagt gttgacaagt agagctatct tccaaaggat gaaaaactac 6901 actatctatg ctgtgtctat cacaataaga attgttttcg gtttcatgct tatcgctttg 6961 atatgggagt ttgatttctc agcattcatg gttctcatca tagctatctt aaacgatgga 7021 acaattatga ccatctctaa ggatagagtt aaacctagtc caacccctga ttcttggaag 7081 ctcaaagaaa tttttgcaac tggagttgtg cttggaggtt atcaggctat tatgacagtt 7141 atctttttct gggctgcaca taagaccgat ttcttttctg atactttcgg tgttagaagt 7201 atcagggata ataaccacga actcatggga gctgtttact tacaagtgtc tattatctca 7261 caggctctta tttttgttac tagatcaagg agttggtctt tcgtggaaag acctggtgct 7321 cttttgatga tagcttttct tattgcacaa ctcatcgcta ctcttattgc tgtttacgca 7381 aactgggaat ttgcaaagat aagaggaatt ggttggggat gggctggagt gatctggctt 7441 tactcaatag ttacttactt ccctttggat gtgtttaagt tcgcaatcag gtatatattg 7501 tctggaaagg cttggcttaa tttgttcgag aacaaaactg cttttactat gaagaaggat 7561 tacggaaagg aagagagaga agctcaatgg gctcttgcac agaggactct ccatggttta 7621 caaccaaagg aggctgttaa tattttccct gaaaagggat catacagaga attgagtgag 7681 atcgctgaac aggcaaagag aagggctgag attgcaagac ttagggaatt gcatacactc 7741 aaaggacacg tggagtcagt tgttaagttg aagggtttag atatagagac accaagtcac 7801 tacacagttt gatactagag taacccgatg ctcctgctct tcctcaagac cttccttgat 7861 tcgccgccgg tatgttctcc gtctgtggta gcgcctttgg aacactctac caacgccgcc 7921 atgaaaggat ctctcatggc cgcaggggac gtgttcttct tacatctggt gttagggcta 7981 tggttactcc agtgaggagg gagaggcaag aggttgctta atgattcgtt tttccggtga 8041 tacgagaact ctttaggttt accgggaagg ttttcccatg aaaatgggat gccaagtgga 8101 tggagaggag ttgccggaga gttgccggag aataggaggg aattggagga ggaggaagag 8161 agtgatcgcc gggttgaaat cttaaccgtc gaggagaatt tgaccgagtt ggatcgtcta 8221 gtaggtacaa ttcgggtcct tggcgaagta tccattcaaa atagtgttta gttttggact 8281 tgagaacttg ttgtctcttt gatctctttt atataaaact ttggacgtgt aggacaaact 8341 tgtcaacata agaaacaaaa tggttgcaac agagaggatg aatttataag ttttcaacac 8401 cgcttttctt attagacgga caacaatcta tagtggagta aatttttatt tttggtaaaa 8461 tggttagtca attcaaatat ctaaattttg tgactcacta acattaacaa atatgcataa 8521 gacataaaaa aaagaaagaa taattcttat gaaacaagaa aaaaaaccta tacaatcaat 8581 ctttaggaat tgacgatgta gaattgtaga tgataaattt tctcaaatat agatgggcct 8641 aatgaagggt gccgcttatt ggatctgacc cattttgagg acaataatat tttcattggt 8701 tataagcctt ttaatcaaaa ttgtcattaa attgatgtct ccctctcggg tcattttcct 8761 ttctccctca caaataatgt agactttagc aatttgctcg ctgtgctttg tctttatatt 8821 tagtaacaca aacattttga cttgtcttgt agagtttttc tcttttattt ttctatccaa 8881 tatgaaaact aaaagtgttc tcgtatacat atattaaaat taaagaaacc tatgaaaaca 8941 ccaatacaaa tgcgatattg ttttcagttc gacgtttcat gtttgttaga aaatttctaa 9001 tgacgtttgt ataaaataga caattaaacg ccaaacacta catctgtgtt ttcgaacaat 9061 attgcgtctg cgtttccttc atctatctct ctcagtgtca caatgtctga actaagagac 9121 agctgtaaac tatcattaag acataaacta ccaaagtatc aagctaatgt aaaaattact 9181 ctcatttcca cgtaacaaat tgagttagct taacatatta gtgaaactag gtttgaattt 9241 tcttcttctt cttccatgca tcctccgaaa aaagggaacc aatcaaaact gtttgcatat 9301 caaactccaa cactttacag caaatgcaat ctataatctg tgatttatcc aataaaaacc 9361 tgtgatttat gtttggctcc agcgatgaaa gtctatgcat gtgatctcta tccaacatga 9421 gtaattgttc agaaaataaa aagtagctga aatgtatcta tataaagaat catccacaag 9481 tactattttc acacactact tcaaaatcac tactcaagaa attactagag tttttggtta 9541 gcgaatgcat ttaatttaga cattgtgtta tgttccactt aaccgcttcc ctgcacttct 9601 ttcaatctat ctctcgatag aaaattgtga tactttgcga cttctatcag aggacttttt 9661 gttttccatg taacaatctg tcattttcga tggggagatt tgcacaaata ggctatttat 9721 gtgtcccaat ttaaatttta accccatgtc gatcagaact tagccacgag caccagaagt 9781 ttgatggata tgtgactttg tcactatccg gtttactaat caagagctat ttttattcaa 9841 aattggatat ctagctaagt ataactggat aatttgcatt aacagattga atatagtgcc 9901 aaacaagaag ggacaattga cttgtcactt tatgaaagat gattcaaaca tgatttttta 9961 tgtactaata tatacatcct actcgaatta aagcgacata ggctcgaagt atgcacattt 10021 agcaatgtaa attaaatcag tttttgaatc aagctaaaag cagacttgca taaggtgggt 10081 ggctggacta gaataaacat cttctctagc acagcttcat aatgtaattt ccataactga 10141 aatcagggtg agacaaaatt ttggtacttt ttcctcacac taagtccatg tttgcaacaa 10201 tttaatacat gaaaccttaa tgttaccctc agattagcct gctactcccc attttcctcg 10261 aaatgctcca acaaaagtta gttttgcaag ttgttgtgta tgtcttgtgc tctatatatg 10321 cccttgtggt gcaagtgtaa cagtacaaca tcatcactca aatcaaagtt tttacttaaa 10381 gaaattagct aaaatactag atggctaggc tctctttatt gttgttactt ctcgtggcag 10441 ctgtggctac cgcagttgat gatcaggcag atcctctcat taggcaggtt actgatggag 10501 atcatcacat gcttaatgct gaacatcact tcactacatt caagactaag ttcggaaagt 10561 cttatgctac acaagaagag catgattaca gatttggtgt tttcagggca aacctcagaa 10621 gggctaaact tcatgcaaaa cttgatccta gtgctgagca tggagttaca aagttttctg 10681 atcttacccc agaagagttc aagagacaat atctcggttt aaaacctctt aggttgccat 10741 caaccgctaa taaggcacct attctcccta cttctgatct tcctgaaaac tttgattgga 10801 gagataaggg agctgttact ccagtgaaaa atcaaggaag ttgtggttct tgctgggctt 10861 tcagtaccac tggtgctctt gaaggtgcac attacttgtc aacaggtgaa ctcgtttctt 10921 tatcagagca acagttggtt gattgtgatc acgtgtgcga tcctgaagag tatggagctt 10981 gtgatgcagg ttgcaacgga ggtcttatga ataacgcttt cgattacatt ttgcaagcag 11041 gaggtgtgca gaccgaaaag gattatccat actctggaag agatgagaca tgtaaatttg 11101 ataagtcaaa ggttgctgca accgtggcta acttctcagt tgtgagtctc gatgaagatc 11161 aaatcgctgc aaatctcgtt aagcatggac ctttagctgt gggaattaac gcaattttca 11221 tgcagacata catcggaggt gtttcttgtc catacatatg cggaaaaaac ttggatcacg 11281 gtgttctttt ggtgggatat ggtgctgctg gatacgctcc tattagattc aaggataagc 11341 cattctggat tatcaaaaat tcttggggag aatcatgggg agaggatggt tattataaga 11401 tttgtagagg aaagaatgtt tgtggtgtgg attcaatggt ttcatctgtg gttgctacta 11461 ctttcacttc atcaaataac tgatactaga gtcgacaagc tctattttct ccataataat 11521 gtgtgagtag ttcccagata agggaattag ggttcctata gggtttcgct catgtgttga 11581 gcatataaga aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt 11641 ctaattccta aaaccaaaat ccagtactaa aatccagatc ccccgaatta aatcggcgtt 11701 aattcag //