BBa_K1537017 1 BBa_K1537017 GSG linker+T2A 2014-09-15T11:00:00Z 2015-05-08T01:10:52Z T2A is from Thosea asigna virus. The 18~22 amino acids 2A self-cleaving oligopeptides can be used for co-expression of multiple, discrete proteins from a single ORF.Based on highly inefficient peptide bond formation between glycine and proline residues within the 2A peptide, placement of 2A peptide sequence as a linker region between tandem cDNA???s allows the stoichiometric translation of multiple unfused protein products. These sequences were first discovered in the foot-and-mouth disease virus (FMDV).And since than many 2A-like sequences have been identified in other viruses and some parasites.To minimize the risk of homologous recombination, it is important to use different 2A peptide sequences if more than two genes are being linked. The 2A peptide system has thus far worked successfully in all eukaryotic systems tested, from mammalian cells, yeast, and plants.In our project,we use F2A(from foot-and-mouth disease virus), P2A(from porcine teschovirus-1) and T2A(from Thosea asigna virus) to achieve our goal.And GSG linker is an oligopeptide of ???Gly-Ser-Gly??? between your protein and 2A peptide to enhance cleavage. false false _1919_ 0 22415 9 In stock false We have to use it by chemosynthesis because the virus is dangerous. false Jie Li annotation2383712 1 GSG linker range2383712 1 1 9 annotation2383711 1 T2A range2383711 1 10 63 BBa_K1537024 1 BBa_K1537024 HPS-PHI 2014-09-16T11:00:00Z 2015-05-08T01:10:52Z Mycobacterium gastri MB19. The ribulose monophosphate (RuMP) pathway is one of the HCHO-fixation pathways found in microorganisms called methylotrophs, which utilize one-carbon compounds as the sole carbon source. The key enzymes of this pathway are 3-hexulose-6-phosphate synthase (HPS), which fixes HCHO to D-ribulose-5-phosphate (Ru5P) to produce D-arabino-3-hexulose 6-phosphate (Hu6P), and 6-phospho-3- hexuloisomerase (PHI), which converts Hu6P to fructose 6-phosphate (F6P).The two key enzymes work in chloroplast both.We will use fusion expression to conduct heterologous expression in tobacco. false false _1919_ 0 22415 9 In stock true We use HPS&PHI as co-expression. false Jie Li annotation2383837 1 PHI range2383837 1 622 1221 annotation2383836 1 HPS range2383836 1 1 621 BBa_K1537009 1 pIGEM009 piGEM002+HPS-PHI+AtFDH+FALDH 2014-10-13T11:00:00Z 2015-05-08T01:10:52Z none. It's Muti-Gene expresion vector based on piGEM002. false false _1919_ 0 22415 9 Not in stock false none. false Jie Li component2419513 1 BBa_K1537029 component2419506 1 BBa_K1537025 component2419523 1 BBa_K1537027 component2419519 1 BBa_K1537020 component2419521 1 BBa_K1537019 component2419504 1 BBa_K1537016 component2419501 1 BBa_K1537024 component2419525 1 BBa_K1537030 component2419498 1 BBa_K1537015 component2419511 1 BBa_K1537026 component2419509 1 BBa_K1537017 component2419515 1 BBa_K1537031 component2419517 1 BBa_K1537028 annotation2419498 1 BBa_K1537015 range2419498 1 1 860 annotation2419519 1 BBa_K1537020 range2419519 1 7821 9522 annotation2419511 1 BBa_K1537026 range2419511 1 3310 4440 annotation2419506 1 BBa_K1537025 range2419506 1 2168 3232 annotation2419523 1 BBa_K1537027 range2419523 1 10401 11483 annotation2419509 1 BBa_K1537017 range2419509 1 3241 3303 annotation2419501 1 BBa_K1537024 range2419501 1 867 2087 annotation2419521 1 BBa_K1537019 range2419521 1 9531 10394 annotation2419504 1 BBa_K1537016 range2419504 1 2096 2161 annotation2419525 1 BBa_K1537030 range2419525 1 11492 11707 annotation2419515 1 BBa_K1537031 range2419515 1 4707 4959 annotation2419517 1 BBa_K1537028 range2419517 1 4966 7812 annotation2419513 1 BBa_K1537029 range2419513 1 4449 4698 BBa_K1537027 1 BBa_K1537027 AdCP 2014-09-16T11:00:00Z 2015-05-08T01:10:52Z Arachis diogoi ATGGCTAGGCTCTCTTTATTGTTGTTACTTCTCGTGGCAGCTGTGGCTACCGCAGTTGATGATCAGGCAGATCCTCTCATTAGGCAGGTTACTGATGGAGATCATCACATGCTTAATGCTGAACATCACTTCACTACATTCAAGACTAAGTTCGGAAAGTCTTATGCTACACAAGAAGAGCATGATTACAGATTTGGTGTTTTCAGGGCAAACCTCAGAAGGGCTAAACTTCATGCAAAACTTGATCCTAGTGCTGAGCATGGAGTTACAAAGTTTTCTGATCTTACCCCAGAAGAGTTCAAGAGACAATATCTCGGTTTAAAACCTCTTAGGTTGCCATCAACCGCTAATAAGGCACCTATTCTCCCTACTTCTGATCTTCCTGAAAACTTTGATTGGAGAGATAAGGGAGCTGTTACTCCAGTGAAAAATCAAGGAAGTTGTGGTTCTTGCTGGGCTTTCAGTACCACTGGTGCTCTTGAAGGTGCACATTACTTGTCAACAGGTGAACTCGTTTCTTTATCAGAGCAACAGTTGGTTGATTGTGATCACGTGTGCGATCCTGAAGAGTATGGAGCTTGTGATGCAGGTTGCAACGGAGGTCTTATGAATAACGCTTTCGATTACATTTTGCAAGCAGGAGGTGTGCAGACCGAAAAGGATTATCCATACTCTGGAAGAGATGAGACATGTAAATTTGATAAGTCAAAGGTTGCTGCAACCGTGGCTAACTTCTCAGTTGTGAGTCTCGATGAAGATCAAATCGCTGCAAATCTCGTTAAGCATGGACCTTTAGCTGTGGGAATTAACGCAATTTTCATGCAGACATACATCGGAGGTGTTTCTTGTCCATACATATGCGGAAAAAACTTGGATCACGGTGTTCTTTTGGTGGGATATGGTGCTGCTGGATACGCTCCTATTAGATTCAAGGATAAGCCATTCTGGATTATCAAAAATTCTTGGGGAGAATCATGGGGAGAGGATGGTTATTATAAGATTTGTAGAGGAAAGAATGTTTGTGGTGTGGATTCAATGGTTTCATCTGTGGTTGCTACTACTTTCACTTCATCAAATAACTGA false false _1919_ 0 22415 9 In stock true We have to remove some restriction endonuclease cutting sites by changing a few bases. false Jie Li annotation2417286 1 AdCP range2417286 1 1 1083 BBa_K1537028 1 BBa_K1537028 AHA2 2014-09-17T11:00:00Z 2015-05-08T01:10:52Z Arabidopsis thaliana AHA2 belongs to the P-type ATPase superfamily of cation-transporting ATPases, pumps protons out of the cell, generating a proton gradient that drives the active transport of nutrients by proton symport.We want to enlarge the tobacco AHA2 to false false _1919_ 0 22415 9 Not in stock true We have to remove some restriction endonuclease cutting sites by changing a few bases. false Jie Li annotation2417289 1 AHA2 range2417289 1 1 2847 BBa_K1537030 1 BBa_K1537030 CaMV35S polyA 2014-09-17T11:00:00Z 2015-05-08T01:10:52Z Cauliflower mosaic virus It???s a kind of terminator derived from cauliflower mosaic virus. false false _1919_ 0 22415 9 Not in stock false None false Jie Li annotation2417606 1 CaMV35S polyA range2417606 1 1 216 BBa_K1537029 1 BBa_K1537029 HSP terminator 2014-09-17T11:00:00Z 2015-05-08T01:10:52Z Arabidopsis thaliana The heat shock protein ( HSP ) terminator increases mRNA levels of both transiently and stably expressed transgenes in plants effectively. false false _1919_ 0 22415 9 Not in stock false none. false Jie Li annotation2417608 1 HSP terminator range2417608 1 1 250 BBa_K1537015 1 BBa_K1537015 35S promoter+translation initiation optimized sequence for dicot+Mass translation enhancer 2014-08-17T11:00:00Z 2015-05-08T01:10:52Z The 35S promoter has a large area of application in genetically modified plants.The translation initiation optimized sequence for dicot is from the paper "translation initiation optimized sequence for dicot"(Nucleic Acids Research Volume 36, Issue 3Pp. 861-871 So Nakagawa1, Yoshihito Niimura2, Takashi Gojobori3,4, Hiroshi Tanaka1,2,* and Kin-ichiro Miura5) The 35S promoter is a strong promoter derived from cauliflower mosaic virus. This constitutive promoter is widely used in transgenic plants to improve the level of the expression of foreign genes effectively."AAAAAAATG" was a kind of preferred nucleotide sequence used in dicot plants（ATG is the initiation codon）to enhance translation initiation.And insertion of ???GCT TCC TCC???(mass translation enhancer) after the initiator codon ATG can augment downstream gene-expression in plants. false false _1919_ 0 22415 9 It's complicated false We have to remove some restriction endonuclease cutting sites by changing a few bases. false Jie Li annotation2393798 1 translation initiation optimized sequence for dicot range2393798 1 843 851 annotation2393797 1 35s promoter range2393797 1 1 842 annotation2393799 1 mass translation enhancer range2393799 1 852 860 BBa_K1537026 1 BBa_K1537026 FALDH 2014-09-16T11:00:00Z 2015-05-08T01:10:52Z Brevibacillus brevis The glutathione-dependent formaldehyde dehydrogenase(FALDH) plays a key role in formaldehyde metabolism. FALDH is identified as an enzyme expressed in the cytoplasm. If we make FALDH over-express in plants, we can enhance plants??? tolerance to HCHO and increase the ability of plants to absorb HCHO. In the process of metabolism of formaldehyde, the formaldehyde may first combined with glutathione (GSH) to form the product of S-hydroxymethyl glutathione (HM-GSH), then FALDH in cytoplasm will catalyzes the formation of a S-formyl glutathione(F-GSH). Next the F-GSH will be hydrolyzed to formate(HCOOH) and GSH by S-formyl glutathione hydrolase (FGH). false false _1919_ 0 22415 9 In stock true none false Jie Li annotation2417288 1 FALDH range2417288 1 1 1131 BBa_K1537016 1 BBa_K1537016 GSG linker+P2A 2014-09-13T11:00:00Z 2015-05-08T01:10:52Z The part is from porcine teschovirus-1. The 18~22 amino acids 2A self-cleaving oligopeptides can be used for co-expression of multiple, discrete proteins from a single ORF.Based on highly inefficient peptide bond formation between glycine and proline residues within the 2A peptide, placement of 2A peptide sequence as a linker region between tandem cDNA???s allows the stoichiometric translation of multiple unfused protein products. These sequences were first discovered in the foot-and-mouth disease virus (FMDV).And since than many 2A-like sequences have been identified in other viruses and some parasites.To minimize the risk of homologous recombination, it is important to use different 2A peptide sequences if more than two genes are being linked. The 2A peptide system has thus far worked successfully in all eukaryotic systems tested, from mammalian cells, yeast, and plants.In our project,we use F2A(from foot-and-mouth disease virus), P2A(from porcine teschovirus-1) and T2A(from Thosea asigna virus) to achieve our goal.And "GSG linker" is an oligopeptide of ???Gly-Ser-Gly??? between your protein and 2A peptide to enhance cleavage. false false _1919_ 0 22415 9 In stock false We have to use this part by synthesis from biological company because the virus is dangerous. false Jie Li annotation2383426 1 GSG linker range2383426 1 1 9 annotation2383427 1 P2A range2383427 1 10 66 BBa_K1537025 1 BBa_K1537025 FDH 2014-09-16T11:00:00Z 2015-05-08T01:10:52Z Arabidopsis thaliana. Formate dehydrogenase is a mitochondrial-localized NAD-requiring enzyme while the HCOOH is getting into the mitochondrial，FDH will oxidize the formic acid into CO2, and reduce NAD+ to NADH with a high degree of specificity.In our project, the heterologous expression of FDH from arabidopsis thaliana in tobacco was completed. false false _1919_ 0 22415 9 In stock true no false Jie Li annotation2417287 1 FDH range2417287 1 1 1065 BBa_K1537019 1 BBa_K1537019 TA29 promoter 2014-09-15T11:00:00Z 2015-05-08T01:10:52Z tissue specific gene of tobacco. TA29 promoter is a tissue-specific(tapetal cells) promoter found in tobacco. false false _1919_ 0 22415 9 It's complicated false no false Jie Li annotation2417290 1 TA29 promoter range2417290 1 1 864 BBa_K1537031 1 BBa_K1537031 NOS ( nopaline synthase ) terminator 2014-09-17T11:00:00Z 2015-05-08T01:10:52Z Agrobacterium tumefasciens It???s a quite common terminator in expression system of plants. false false _1919_ 0 22415 9 Not in stock false None. false Jie Li annotation2417607 1 NOS terminator range2417607 1 1 253 BBa_K1537020 1 BBa_K1537020 GC1 promoter 2014-09-16T11:00:00Z 2015-05-08T01:10:52Z Arabidopsis. The GC1 promoter drives strong reporter expression in guard cells of Arabidopsis and tobacco plants. It provides a potent research tool for targeted guard cell expression. false false _1919_ 0 22415 9 It's complicated false no false Jie Li annotation2417291 1 GC1 promoter range2417291 1 1 1702 BBa_K1537027_sequence 1 atggctaggctctctttattgttgttacttctcgtggcagctgtggctaccgcagttgatgatcaggcagatcctctcattaggcaggttactgatggagatcatcacatgcttaatgctgaacatcacttcactacattcaagactaagttcggaaagtcttatgctacacaagaagagcatgattacagatttggtgttttcagggcaaacctcagaagggctaaacttcatgcaaaacttgatcctagtgctgagcatggagttacaaagttttctgatcttaccccagaagagttcaagagacaatatctcggtttaaaacctcttaggttgccatcaaccgctaataaggcacctattctccctacttctgatcttcctgaaaactttgattggagagataagggagctgttactccagtgaaaaatcaaggaagttgtggttcttgctgggctttcagtaccactggtgctcttgaaggtgcacattacttgtcaacaggtgaactcgtttctttatcagagcaacagttggttgattgtgatcacgtgtgcgatcctgaagagtatggagcttgtgatgcaggttgcaacggaggtcttatgaataacgctttcgattacattttgcaagcaggaggtgtgcagaccgaaaaggattatccatactctggaagagatgagacatgtaaatttgataagtcaaaggttgctgcaaccgtggctaacttctcagttgtgagtctcgatgaagatcaaatcgctgcaaatctcgttaagcatggacctttagctgtgggaattaacgcaattttcatgcagacatacatcggaggtgtttcttgtccatacatatgcggaaaaaacttggatcacggtgttcttttggtgggatatggtgctgctggatacgctcctattagattcaaggataagccattctggattatcaaaaattcttggggagaatcatggggagaggatggttattataagatttgtagaggaaagaatgtttgtggtgtggattcaatggtttcatctgtggttgctactactttcacttcatcaaataactga BBa_K1537024_sequence 1 atgaaacttcaggttgctatcgatcttttgtcaacagaagctgcattagagcttgctggaaaggtggcagaatatgttgatattatcgagttaggtactcctcttatagaagctgagggattgtcagtgattacagctgttaagaaagcacatccagataagatcgttttcgctgatatgaagactatggatgcaggtgaacttgaggctgatatagcattcaaagctggtgcagatttggtgacagttctcggaagtgctgatgattctaccattgctggagcagttaaggctgcacaagctcataataagggtgttgtggttgatttgattggtatagaagataaggctacaagagcacaagaggtgagggctctcggtgcaaaatttgttgaaatgcacgctggattagatgagcaggcaaagcctggattcgatcttaatggtcttcttgctgctggagaaaaggctagagtgcctttctctgttgcaggaggtgtgaaggttgctacaattccagcagttcaaaaagctggtgcagaggtggctgttgcaggaggtgctatctacggagctgctgatcctgctgctgctgctaaagaattgagagctgcaatagctatgactcaggctgctgaagctgatggtgcagttaaggtggttggagatgatattaccaataacttgtcactcgtgagggatgaagttgctgatactgctgcaaaagttgatcctgagcaggtggctgttcttgcaagacaaatcgttcagcctggtagggtgtttgttgctggagcaggtagatcaggattagttcttagaatggctgcaatgaggttaatgcatttcggtcttaccgtgcacgttgctggagatactacaacccctgctatatctgctggagatttgttgctcgttgcttctggatcaggtactacatcaggagtggttaaaagtgctgaaactgcaaagaaagctggagcaagaattgctgcttttactactaatcctgattctccattggctggactcgctgatgcagtggttataattccagctgcacaaaagactgatcatggttctcacatttcaaggcaatatgctggaagtttgtttgaacaggtgctcttcgtggttacagaggctgttttccagtctttatgggatcacacagaggtggaggctgaagaattatggacaagacacgcaaacttagagtga BBa_K1537017_sequence 1 ggtagtggagagggtagaggatctcttttgacatgtggagatgttgaagagaaccctggacca BBa_K1537020_sequence 1 taacccgatgctcctgctcttcctcaagaccttccttgattcgccgccggtatgttctccgtctgtggtagcgcctttggaacactctaccaacgccgccatgaaaggatctctcatggccgcaggggacgtgttcttcttacatctggtgttagggctatggttactccagtgaggagggagaggcaagaggttgcttaatgattcgtttttccggtgatacgagaactctttaggtttaccgggaaggttttcccatgaaaatgggatgccaagtggatggagaggagttgccggagagttgccggagaataggagggaattggaggaggaggaagagagtgatcgccgggttgaaatcttaaccgtcgaggagaatttgaccgagttggatcgtctagtaggtacaattcgggtccttggcgaagtatccattcaaaatagtgtttagttttggacttgagaacttgttgtctctttgatctcttttatataaaactttggacgtgtaggacaaacttgtcaacataagaaacaaaatggttgcaacagagaggatgaatttataagttttcaacaccgcttttcttattagacggacaacaatctatagtggagtaaatttttatttttggtaaaatggttagtcaattcaaatatctaaattttgtgactcactaacattaacaaatatgcataagacataaaaaaaagaaagaataattcttatgaaacaagaaaaaaaacctatacaatcaatctttaggaattgacgatgtagaattgtagatgataaattttctcaaatatagatgggcctaatgaagggtgccgcttattggatctgacccattttgaggacaataatattttcattggttataagccttttaatcaaaattgtcattaaattgatgtctccctctcgggtcattttcctttctccctcacaaataatgtagactttagcaatttgctcgctgtgctttgtctttatatttagtaacacaaacattttgacttgtcttgtagagtttttctcttttatttttctatccaatatgaaaactaaaagtgttctcgtatacatatattaaaattaaagaaacctatgaaaacaccaatacaaatgcgatattgttttcagttcgacgtttcatgtttgttagaaaatttctaatgacgtttgtataaaatagacaattaaacgccaaacactacatctgtgttttcgaacaatattgcgtctgcgtttccttcatctatctctctcagtgtcacaatgtctgaactaagagacagctgtaaactatcattaagacataaactaccaaagtatcaagctaatgtaaaaattactctcatttccacgtaacaaattgagttagcttaacatattagtgaaactaggtttgaattttcttcttcttcttccatgcatcctccgaaaaaagggaaccaatcaaaactgtttgcatatcaaactccaacactttacagcaaatgcaatctataatctgtgatttatccaataaaaacctgtgatttatgtttggctccagcgatgaaagtctatgcatgtgatctctatccaacatgagtaattgttcagaaaataaaaagtagctgaaatgtatctatataaagaatcatccacaagtactattttcacacactacttcaaaatcactactcaagaaat BBa_K1537009_sequence 1 agatttgccttttcaatttcagaaagaatgctaacccacagatggttagagaggcttacgcagcaggtctcatcaagacgatctacccgagcaataatctccaggaaatcaaataccttcccaagaaggttaaagatgcagtcaaaagattcaggactaactgcatcaagaacacagagaaagatatatttctcaagatcagaagtactattccagtatggacgattcaaggcttgcttcacaaaccaaggcaagtaatagagattggagtctctaaaaaggtagttcccactgaatcaaaggccatggagtcaaagattcaaatagaggacctaacagaactcgccgtaaagactggcgaacagttcatacagagtctcttacgactcaatgacaagaagaaaatcttcgtcaacatggtggagcacgacacacttgtctactccaaaaatatcaaagatacagtctcagaagaccaaagggcaattgagacttttcaacaaagggtaatatccggaaacctcctcggattccattgcccagctatctgtcactttattgtgaagatagtggaaaaggaaggtggctcctacaaatgccatcattgcgataaaggaaaggccatcgttgaagatgcctctgccgacagtggtcccaaagatggacccccacccacgaggagcatcgtggaaaaagaagacgttccaaccacgtcttcaaagcaagtggattgatgtgatatctccactgacgtaagggatgacgcacaatcccactatccttcgcaagacccttcctctatataaggaagttcatttcatttggagagaacacgggggactaaaaaaatggcttcctcctactagatgaaacttcaggttgctatcgatcttttgtcaacagaagctgcattagagcttgctggaaaggtggcagaatatgttgatattatcgagttaggtactcctcttatagaagctgagggattgtcagtgattacagctgttaagaaagcacatccagataagatcgttttcgctgatatgaagactatggatgcaggtgaacttgaggctgatatagcattcaaagctggtgcagatttggtgacagttctcggaagtgctgatgattctaccattgctggagcagttaaggctgcacaagctcataataagggtgttgtggttgatttgattggtatagaagataaggctacaagagcacaagaggtgagggctctcggtgcaaaatttgttgaaatgcacgctggattagatgagcaggcaaagcctggattcgatcttaatggtcttcttgctgctggagaaaaggctagagtgcctttctctgttgcaggaggtgtgaaggttgctacaattccagcagttcaaaaagctggtgcagaggtggctgttgcaggaggtgctatctacggagctgctgatcctgctgctgctgctaaagaattgagagctgcaatagctatgactcaggctgctgaagctgatggtgcagttaaggtggttggagatgatattaccaataacttgtcactcgtgagggatgaagttgctgatactgctgcaaaagttgatcctgagcaggtggctgttcttgcaagacaaatcgttcagcctggtagggtgtttgttgctggagcaggtagatcaggattagttcttagaatggctgcaatgaggttaatgcatttcggtcttaccgtgcacgttgctggagatactacaacccctgctatatctgctggagatttgttgctcgttgcttctggatcaggtactacatcaggagtggttaaaagtgctgaaactgcaaagaaagctggagcaagaattgctgcttttactactaatcctgattctccattggctggactcgctgatgcagtggttataattccagctgcacaaaagactgatcatggttctcacatttcaaggcaatatgctggaagtttgtttgaacaggtgctcttcgtggttacagaggctgttttccagtctttatgggatcacacagaggtggaggctgaagaattatggacaagacacgcaaacttagagtgatactagagggtagtggagcaacaaacttctcattattaaagcaagcaggagatgtggaggaaaatcctggtccatactagatgtcttcaggagatagtaagaaaattgttggtgtgttttataaggctaacgagtacgcaactaaaaatccaaacttccttggatgtgttgagaatgctcttggtattagagattggttggaatcacaaggacatcagtatatcgttacagatgataaggagggtccagattgcgaacttgagaaacatattcctgatctccacgttttaatctctacaccatttcatcctgcttacgtgaccgcagaaaggattaagaaagctaagaacttgaaacttttgctcaccgcaggaatcggttcagatcacattgatcttcaagctgctgctgctgctggacttactgttgctgaagtgactggtagtaacgttgtgtctgttgcagaagatgagttgatgagaattttgattcttatgaggaacttcgttcctggatataaccaagttgtgaagggagagtggaatgtggcaggtatcgcttatagagcatacgatcttgaaggaaaaaccataggtactgttggagctggtagaattggaaagttacttttgcagaggttgaaaccatttggttgtaatctcttataccatgataggctccaaatggctcctgaattagagaaggaaacaggtgcaaaattcgttgaggatcttaatgagatgttgccaaagtgcgatgttatagtgattaacatgcctctcacagagaaaaccagaggaatgttcaataaggaactcataggaaaattaaagaaaggtgttttgatcgtgaataacgctagaggagctattatggaaaggcaggctgttgtggatgcagttgaatcaggacacatcggaggttatagtggagatgtttgggaccctcaaccagctcctaaggatcatccatggagatacatgcctaaccaggctatgactccacacacatctggaactacaatcgatgctcaactcagatatgcagctggaactaaggatatgttagagaggtacttcaaaggtgaagattttccaaccgagaattacatagtgaaggatggtgaacttgctccacaatacaggtactagagggtagtggagagggtagaggatctcttttgacatgtggagatgttgaagagaaccctggaccatactagatgggagcagtgacttaccaaggaaaaaattctatcgctgttaagaaagtggatgcaccatcaattcaggatagagaggatgttattatcaggatcacttctacagctatatgtggatctgatcttcatctttatcagggtaacttccctttgccaataggatacgttattggtcacgaacctatgggaattgttgaagaagtgggtccagatgttacagctgtgaagaaaggagatagagttgtgatcccttttaccgttgcatgtggtaaatgccaatattgtgatcatcatcttgaatcacagtgcgataatagtaacccacattatgatagtggaggtttattcggatactctgagaagtatggtaattaccctggaggtcaagctgaatatctcagggttccatttggaaactacacacctttcaaaattccagatgattgcgaattggaggatgaacagcttttgtttctctctgatgtgttacctaccgcttactggtctgttgagcatgcaggagtgaagaaaggagatacagttatcgtgctcggatgtggtccagttggacttatggctcaacagttcgcatggcaaaagggtgctgaaagagttattgcagtggattatatcgattacagacttaggcacgctaaaaggatgaatggtgttgaggtgtttgatttcactgaagatcctgatatgggtgagacacttaaagaattgaccaaaggaggtgcagatgttgtgattgattgtgttggtatggatggaaagaagtctcctttggagaagatcgaacaaaaacttaaacttcaaggaggtactataggtccaatccaaatagctacaaaggcagttagaaaaaggggaaccgttcagatgactggtgtgtatggaggtctttacaatatgtttcctcttggagctttctttgcaagaaacgttacacttaaaatgggacaagctccagcaaggggttatatgtcaaagttgtaccagaaagtgaccagtggagagattgatcctagagctataattactcacaaactcccattagatgaagctgcacacgcataccatatcttcaacgataaacaggatgattgcataaaagtgattctcaagccttactagagatatgaagatgaagatgaaatatttggtgtgtcaaataaaaaccttgtgtggttaagtttgtgtttttttcttggcttgttgtgttatgaatttgtggctttttctaatattaaatgaatgtaagatcacattataatgaataaacaaatgtttctataatccattgtgaatgttttgttggatctcttctgcaccatataactactgtatgtgctatggtatggactatggaatatgattaaagataagtactagagcgttcaaacatttggcaataaagtttcttaagattgaatcctgttgccggtcttgcgatgattatcatataatttctgttgaattacgttaagcatgtaataattaacatgtaatgcatgacgttatttatgagatgggtttttatgattagagtcccgcaattatacatttaatacgcgatagaaaacaaaatatagcgcgcaaactaggataaattatcgcgcgcggtgtcatctatgttactagatcgggtactagatgtcatcattagaagatattaagaacgagactgtggatttggagaagatacctatagaggaagtgtttcagcaactcaagtgtagtagagagggtttgactacacaagaaggagaggataggatacagatttttggtccaaacaaacttgaagagaagaaagagtctaaacttttgaaatttcttggattcatgtggaatcctttgagttgggttatggaaatggctgcaatcatggctatagcacttgctaacggagatggtagacctccagattggcaagattttgtgggtattatctgtctcttagttatcaattcaactattagtttcatcgaagagaataacgctggaaacgctgctgctgctcttatggctggtcttgcaccaaagacaaaagttcttagagatggaaagtggtctgaacaagaggctgcaattctcgttcctggagatattgtgtcaattaaattaggagatattattccagcagatgctagacttttggagggagatcctttgaaggttgatcagtctgctcttacaggagagtcattgccagttaccaagcatcctggtcaagaagtgttcagtggatctacttgcaaacagggtgaaattgaggctgttgtgatcgcaaccggagttcatactttctttggaaaggctgcacacctcgttgattcaacaaatcaagtgggacactttcagaaagttcttaccgcaatcggtaacttctgtatttgctctatcgctattggaatggttattgaaatcatagtgatgtatccaatccaaagaaggaaatacagagatggtatagataacctcttagttcttttgattggaggtattccaatcgcaatgcctactgttttgtctgtgacaatggctattggaagtcatagactctctcaacagggtgctataacaaaaaggatgaccgcaattgaagagatggctggtatggatgttctttgttctgataagaccggaactctcacactcaacaaactttcagtggataagaacttggttgaggtgttttgcaagggagttgaaaaagatcaagtgctcttattcgctgcaatggcttcaagagttgaaaatcaggatgctatcgatgctgcaatggtgggaatgttggctgatccaaaagaggcaagagctggaattagggaggttcattttcttccattcaatcctgtggataagagaaccgctttgacttatatcgatggaagtggtaactggcacagggtttctaagggtgctcctgagcaaattcttgaattggcaaaggcttctaacgatctcagtaagaaagttttaagtattatcgataagtacgctgagagaggactcaggtctttagcagtggctagacaggttgtgccagagaagactaaagaatctcctggtgctccttgggagtttgttggtcttttgcctcttttcgatcctccaaggcatgattctgctgaaactatcagaagggctctcaatcttggagttaatgtgaagatgatcacaggagatcaacttgctatcggtaaagaaaccggaagaaggttgggaatgggtactaatatgtatccttcttcagctctcttaggtactcacaaggatgctaacctcgcatctattccagttgaagagttaatcgagaaagctgatggttttgcaggagttttccctgagcataagtacgaaatcgtgaagaaacttcaggaaagaaagcatattgttggtatgacaggagatggtgttaatgatgcaccagctttgaagaaagctgatataggaattgcagttgctgatgcaactgatgctgcaagaggtgcttctgatattgtgcttacagagcctggattgtctgttataatttcagcagtgttgacaagtagagctatcttccaaaggatgaaaaactacactatctatgctgtgtctatcacaataagaattgttttcggtttcatgcttatcgctttgatatgggagtttgatttctcagcattcatggttctcatcatagctatcttaaacgatggaacaattatgaccatctctaaggatagagttaaacctagtccaacccctgattcttggaagctcaaagaaatttttgcaactggagttgtgcttggaggttatcaggctattatgacagttatctttttctgggctgcacataagaccgatttcttttctgatactttcggtgttagaagtatcagggataataaccacgaactcatgggagctgtttacttacaagtgtctattatctcacaggctcttatttttgttactagatcaaggagttggtctttcgtggaaagacctggtgctcttttgatgatagcttttcttattgcacaactcatcgctactcttattgctgtttacgcaaactgggaatttgcaaagataagaggaattggttggggatgggctggagtgatctggctttactcaatagttacttacttccctttggatgtgtttaagttcgcaatcaggtatatattgtctggaaaggcttggcttaatttgttcgagaacaaaactgcttttactatgaagaaggattacggaaaggaagagagagaagctcaatgggctcttgcacagaggactctccatggtttacaaccaaaggaggctgttaatattttccctgaaaagggatcatacagagaattgagtgagatcgctgaacaggcaaagagaagggctgagattgcaagacttagggaattgcatacactcaaaggacacgtggagtcagttgttaagttgaagggtttagatatagagacaccaagtcactacacagtttgatactagagtaacccgatgctcctgctcttcctcaagaccttccttgattcgccgccggtatgttctccgtctgtggtagcgcctttggaacactctaccaacgccgccatgaaaggatctctcatggccgcaggggacgtgttcttcttacatctggtgttagggctatggttactccagtgaggagggagaggcaagaggttgcttaatgattcgtttttccggtgatacgagaactctttaggtttaccgggaaggttttcccatgaaaatgggatgccaagtggatggagaggagttgccggagagttgccggagaataggagggaattggaggaggaggaagagagtgatcgccgggttgaaatcttaaccgtcgaggagaatttgaccgagttggatcgtctagtaggtacaattcgggtccttggcgaagtatccattcaaaatagtgtttagttttggacttgagaacttgttgtctctttgatctcttttatataaaactttggacgtgtaggacaaacttgtcaacataagaaacaaaatggttgcaacagagaggatgaatttataagttttcaacaccgcttttcttattagacggacaacaatctatagtggagtaaatttttatttttggtaaaatggttagtcaattcaaatatctaaattttgtgactcactaacattaacaaatatgcataagacataaaaaaaagaaagaataattcttatgaaacaagaaaaaaaacctatacaatcaatctttaggaattgacgatgtagaattgtagatgataaattttctcaaatatagatgggcctaatgaagggtgccgcttattggatctgacccattttgaggacaataatattttcattggttataagccttttaatcaaaattgtcattaaattgatgtctccctctcgggtcattttcctttctccctcacaaataatgtagactttagcaatttgctcgctgtgctttgtctttatatttagtaacacaaacattttgacttgtcttgtagagtttttctcttttatttttctatccaatatgaaaactaaaagtgttctcgtatacatatattaaaattaaagaaacctatgaaaacaccaatacaaatgcgatattgttttcagttcgacgtttcatgtttgttagaaaatttctaatgacgtttgtataaaatagacaattaaacgccaaacactacatctgtgttttcgaacaatattgcgtctgcgtttccttcatctatctctctcagtgtcacaatgtctgaactaagagacagctgtaaactatcattaagacataaactaccaaagtatcaagctaatgtaaaaattactctcatttccacgtaacaaattgagttagcttaacatattagtgaaactaggtttgaattttcttcttcttcttccatgcatcctccgaaaaaagggaaccaatcaaaactgtttgcatatcaaactccaacactttacagcaaatgcaatctataatctgtgatttatccaataaaaacctgtgatttatgtttggctccagcgatgaaagtctatgcatgtgatctctatccaacatgagtaattgttcagaaaataaaaagtagctgaaatgtatctatataaagaatcatccacaagtactattttcacacactacttcaaaatcactactcaagaaattactagagtttttggttagcgaatgcatttaatttagacattgtgttatgttccacttaaccgcttccctgcacttctttcaatctatctctcgatagaaaattgtgatactttgcgacttctatcagaggactttttgttttccatgtaacaatctgtcattttcgatggggagatttgcacaaataggctatttatgtgtcccaatttaaattttaaccccatgtcgatcagaacttagccacgagcaccagaagtttgatggatatgtgactttgtcactatccggtttactaatcaagagctatttttattcaaaattggatatctagctaagtataactggataatttgcattaacagattgaatatagtgccaaacaagaagggacaattgacttgtcactttatgaaagatgattcaaacatgattttttatgtactaatatatacatcctactcgaattaaagcgacataggctcgaagtatgcacatttagcaatgtaaattaaatcagtttttgaatcaagctaaaagcagacttgcataaggtgggtggctggactagaataaacatcttctctagcacagcttcataatgtaatttccataactgaaatcagggtgagacaaaattttggtactttttcctcacactaagtccatgtttgcaacaatttaatacatgaaaccttaatgttaccctcagattagcctgctactccccattttcctcgaaatgctccaacaaaagttagttttgcaagttgttgtgtatgtcttgtgctctatatatgcccttgtggtgcaagtgtaacagtacaacatcatcactcaaatcaaagtttttacttaaagaaattagctaaaatactagatggctaggctctctttattgttgttacttctcgtggcagctgtggctaccgcagttgatgatcaggcagatcctctcattaggcaggttactgatggagatcatcacatgcttaatgctgaacatcacttcactacattcaagactaagttcggaaagtcttatgctacacaagaagagcatgattacagatttggtgttttcagggcaaacctcagaagggctaaacttcatgcaaaacttgatcctagtgctgagcatggagttacaaagttttctgatcttaccccagaagagttcaagagacaatatctcggtttaaaacctcttaggttgccatcaaccgctaataaggcacctattctccctacttctgatcttcctgaaaactttgattggagagataagggagctgttactccagtgaaaaatcaaggaagttgtggttcttgctgggctttcagtaccactggtgctcttgaaggtgcacattacttgtcaacaggtgaactcgtttctttatcagagcaacagttggttgattgtgatcacgtgtgcgatcctgaagagtatggagcttgtgatgcaggttgcaacggaggtcttatgaataacgctttcgattacattttgcaagcaggaggtgtgcagaccgaaaaggattatccatactctggaagagatgagacatgtaaatttgataagtcaaaggttgctgcaaccgtggctaacttctcagttgtgagtctcgatgaagatcaaatcgctgcaaatctcgttaagcatggacctttagctgtgggaattaacgcaattttcatgcagacatacatcggaggtgtttcttgtccatacatatgcggaaaaaacttggatcacggtgttcttttggtgggatatggtgctgctggatacgctcctattagattcaaggataagccattctggattatcaaaaattcttggggagaatcatggggagaggatggttattataagatttgtagaggaaagaatgtttgtggtgtggattcaatggtttcatctgtggttgctactactttcacttcatcaaataactgatactagagtcgacaagctctattttctccataataatgtgtgagtagttcccagataagggaattagggttcctatagggtttcgctcatgtgttgagcatataagaaacccttagtatgtatttgtatttgtaaaatacttctatcaataaaatttctaattcctaaaaccaaaatccagtactaaaatccagatcccccgaattaaatcggcgttaattcag BBa_K1537026_sequence 1 atgggagcagtgacttaccaaggaaaaaattctatcgctgttaagaaagtggatgcaccatcaattcaggatagagaggatgttattatcaggatcacttctacagctatatgtggatctgatcttcatctttatcagggtaacttccctttgccaataggatacgttattggtcacgaacctatgggaattgttgaagaagtgggtccagatgttacagctgtgaagaaaggagatagagttgtgatcccttttaccgttgcatgtggtaaatgccaatattgtgatcatcatcttgaatcacagtgcgataatagtaacccacattatgatagtggaggtttattcggatactctgagaagtatggtaattaccctggaggtcaagctgaatatctcagggttccatttggaaactacacacctttcaaaattccagatgattgcgaattggaggatgaacagcttttgtttctctctgatgtgttacctaccgcttactggtctgttgagcatgcaggagtgaagaaaggagatacagttatcgtgctcggatgtggtccagttggacttatggctcaacagttcgcatggcaaaagggtgctgaaagagttattgcagtggattatatcgattacagacttaggcacgctaaaaggatgaatggtgttgaggtgtttgatttcactgaagatcctgatatgggtgagacacttaaagaattgaccaaaggaggtgcagatgttgtgattgattgtgttggtatggatggaaagaagtctcctttggagaagatcgaacaaaaacttaaacttcaaggaggtactataggtccaatccaaatagctacaaaggcagttagaaaaaggggaaccgttcagatgactggtgtgtatggaggtctttacaatatgtttcctcttggagctttctttgcaagaaacgttacacttaaaatgggacaagctccagcaaggggttatatgtcaaagttgtaccagaaagtgaccagtggagagattgatcctagagctataattactcacaaactcccattagatgaagctgcacacgcataccatatcttcaacgataaacaggatgattgcataaaagtgattctcaagcct BBa_K1537025_sequence 1 atgtcttcaggagatagtaagaaaattgttggtgtgttttataaggctaacgagtacgcaactaaaaatccaaacttccttggatgtgttgagaatgctcttggtattagagattggttggaatcacaaggacatcagtatatcgttacagatgataaggagggtccagattgcgaacttgagaaacatattcctgatctccacgttttaatctctacaccatttcatcctgcttacgtgaccgcagaaaggattaagaaagctaagaacttgaaacttttgctcaccgcaggaatcggttcagatcacattgatcttcaagctgctgctgctgctggacttactgttgctgaagtgactggtagtaacgttgtgtctgttgcagaagatgagttgatgagaattttgattcttatgaggaacttcgttcctggatataaccaagttgtgaagggagagtggaatgtggcaggtatcgcttatagagcatacgatcttgaaggaaaaaccataggtactgttggagctggtagaattggaaagttacttttgcagaggttgaaaccatttggttgtaatctcttataccatgataggctccaaatggctcctgaattagagaaggaaacaggtgcaaaattcgttgaggatcttaatgagatgttgccaaagtgcgatgttatagtgattaacatgcctctcacagagaaaaccagaggaatgttcaataaggaactcataggaaaattaaagaaaggtgttttgatcgtgaataacgctagaggagctattatggaaaggcaggctgttgtggatgcagttgaatcaggacacatcggaggttatagtggagatgtttgggaccctcaaccagctcctaaggatcatccatggagatacatgcctaaccaggctatgactccacacacatctggaactacaatcgatgctcaactcagatatgcagctggaactaaggatatgttagagaggtacttcaaaggtgaagattttccaaccgagaattacatagtgaaggatggtgaacttgctccacaatacagg BBa_K1537019_sequence 1 tttttggttagcgaatgcatttaatttagacattgtgttatgttccacttaaccgcttccctgcacttctttcaatctatctctcgatagaaaattgtgatactttgcgacttctatcagaggactttttgttttccatgtaacaatctgtcattttcgatggggagatttgcacaaataggctatttatgtgtcccaatttaaattttaaccccatgtcgatcagaacttagccacgagcaccagaagtttgatggatatgtgactttgtcactatccggtttactaatcaagagctatttttattcaaaattggatatctagctaagtataactggataatttgcattaacagattgaatatagtgccaaacaagaagggacaattgacttgtcactttatgaaagatgattcaaacatgattttttatgtactaatatatacatcctactcgaattaaagcgacataggctcgaagtatgcacatttagcaatgtaaattaaatcagtttttgaatcaagctaaaagcagacttgcataaggtgggtggctggactagaataaacatcttctctagcacagcttcataatgtaatttccataactgaaatcagggtgagacaaaattttggtactttttcctcacactaagtccatgtttgcaacaatttaatacatgaaaccttaatgttaccctcagattagcctgctactccccattttcctcgaaatgctccaacaaaagttagttttgcaagttgttgtgtatgtcttgtgctctatatatgcccttgtggtgcaagtgtaacagtacaacatcatcactcaaatcaaagtttttacttaaagaaattagctaaaa BBa_K1537031_sequence 1 cgttcaaacatttggcaataaagtttcttaagattgaatcctgttgccggtcttgcgatgattatcatataatttctgttgaattacgttaagcatgtaataattaacatgtaatgcatgacgttatttatgagatgggtttttatgattagagtcccgcaattatacatttaatacgcgatagaaaacaaaatatagcgcgcaaactaggataaattatcgcgcgcggtgtcatctatgttactagatcggg BBa_K1537016_sequence 1 ggtagtggagcaacaaacttctcattattaaagcaagcaggagatgtggaggaaaatcctggtcca BBa_K1537029_sequence 1 atatgaagatgaagatgaaatatttggtgtgtcaaataaaaaccttgtgtggttaagtttgtgtttttttcttggcttgttgtgttatgaatttgtggctttttctaatattaaatgaatgtaagatcacattataatgaataaacaaatgtttctataatccattgtgaatgttttgttggatctcttctgcaccatataactactgtatgtgctatggtatggactatggaatatgattaaagataag BBa_K1537030_sequence 1 tcgacaagctctattttctccataataatgtgtgagtagttcccagataagggaattagggttcctatagggtttcgctcatgtgttgagcatataagaaacccttagtatgtatttgtatttgtaaaatacttctatcaataaaatttctaattcctaaaaccaaaatccagtactaaaatccagatcccccgaattaaatcggcgttaattcag BBa_K1537015_sequence 1 agatttgccttttcaatttcagaaagaatgctaacccacagatggttagagaggcttacgcagcaggtctcatcaagacgatctacccgagcaataatctccaggaaatcaaataccttcccaagaaggttaaagatgcagtcaaaagattcaggactaactgcatcaagaacacagagaaagatatatttctcaagatcagaagtactattccagtatggacgattcaaggcttgcttcacaaaccaaggcaagtaatagagattggagtctctaaaaaggtagttcccactgaatcaaaggccatggagtcaaagattcaaatagaggacctaacagaactcgccgtaaagactggcgaacagttcatacagagtctcttacgactcaatgacaagaagaaaatcttcgtcaacatggtggagcacgacacacttgtctactccaaaaatatcaaagatacagtctcagaagaccaaagggcaattgagacttttcaacaaagggtaatatccggaaacctcctcggattccattgcccagctatctgtcactttattgtgaagatagtggaaaaggaaggtggctcctacaaatgccatcattgcgataaaggaaaggccatcgttgaagatgcctctgccgacagtggtcccaaagatggacccccacccacgaggagcatcgtggaaaaagaagacgttccaaccacgtcttcaaagcaagtggattgatgtgatatctccactgacgtaagggatgacgcacaatcccactatccttcgcaagacccttcctctatataaggaagttcatttcatttggagagaacacgggggactaaaaaaatggcttcctcc BBa_K1537028_sequence 1 atgtcatcattagaagatattaagaacgagactgtggatttggagaagatacctatagaggaagtgtttcagcaactcaagtgtagtagagagggtttgactacacaagaaggagaggataggatacagatttttggtccaaacaaacttgaagagaagaaagagtctaaacttttgaaatttcttggattcatgtggaatcctttgagttgggttatggaaatggctgcaatcatggctatagcacttgctaacggagatggtagacctccagattggcaagattttgtgggtattatctgtctcttagttatcaattcaactattagtttcatcgaagagaataacgctggaaacgctgctgctgctcttatggctggtcttgcaccaaagacaaaagttcttagagatggaaagtggtctgaacaagaggctgcaattctcgttcctggagatattgtgtcaattaaattaggagatattattccagcagatgctagacttttggagggagatcctttgaaggttgatcagtctgctcttacaggagagtcattgccagttaccaagcatcctggtcaagaagtgttcagtggatctacttgcaaacagggtgaaattgaggctgttgtgatcgcaaccggagttcatactttctttggaaaggctgcacacctcgttgattcaacaaatcaagtgggacactttcagaaagttcttaccgcaatcggtaacttctgtatttgctctatcgctattggaatggttattgaaatcatagtgatgtatccaatccaaagaaggaaatacagagatggtatagataacctcttagttcttttgattggaggtattccaatcgcaatgcctactgttttgtctgtgacaatggctattggaagtcatagactctctcaacagggtgctataacaaaaaggatgaccgcaattgaagagatggctggtatggatgttctttgttctgataagaccggaactctcacactcaacaaactttcagtggataagaacttggttgaggtgttttgcaagggagttgaaaaagatcaagtgctcttattcgctgcaatggcttcaagagttgaaaatcaggatgctatcgatgctgcaatggtgggaatgttggctgatccaaaagaggcaagagctggaattagggaggttcattttcttccattcaatcctgtggataagagaaccgctttgacttatatcgatggaagtggtaactggcacagggtttctaagggtgctcctgagcaaattcttgaattggcaaaggcttctaacgatctcagtaagaaagttttaagtattatcgataagtacgctgagagaggactcaggtctttagcagtggctagacaggttgtgccagagaagactaaagaatctcctggtgctccttgggagtttgttggtcttttgcctcttttcgatcctccaaggcatgattctgctgaaactatcagaagggctctcaatcttggagttaatgtgaagatgatcacaggagatcaacttgctatcggtaaagaaaccggaagaaggttgggaatgggtactaatatgtatccttcttcagctctcttaggtactcacaaggatgctaacctcgcatctattccagttgaagagttaatcgagaaagctgatggttttgcaggagttttccctgagcataagtacgaaatcgtgaagaaacttcaggaaagaaagcatattgttggtatgacaggagatggtgttaatgatgcaccagctttgaagaaagctgatataggaattgcagttgctgatgcaactgatgctgcaagaggtgcttctgatattgtgcttacagagcctggattgtctgttataatttcagcagtgttgacaagtagagctatcttccaaaggatgaaaaactacactatctatgctgtgtctatcacaataagaattgttttcggtttcatgcttatcgctttgatatgggagtttgatttctcagcattcatggttctcatcatagctatcttaaacgatggaacaattatgaccatctctaaggatagagttaaacctagtccaacccctgattcttggaagctcaaagaaatttttgcaactggagttgtgcttggaggttatcaggctattatgacagttatctttttctgggctgcacataagaccgatttcttttctgatactttcggtgttagaagtatcagggataataaccacgaactcatgggagctgtttacttacaagtgtctattatctcacaggctcttatttttgttactagatcaaggagttggtctttcgtggaaagacctggtgctcttttgatgatagcttttcttattgcacaactcatcgctactcttattgctgtttacgcaaactgggaatttgcaaagataagaggaattggttggggatgggctggagtgatctggctttactcaatagttacttacttccctttggatgtgtttaagttcgcaatcaggtatatattgtctggaaaggcttggcttaatttgttcgagaacaaaactgcttttactatgaagaaggattacggaaaggaagagagagaagctcaatgggctcttgcacagaggactctccatggtttacaaccaaaggaggctgttaatattttccctgaaaagggatcatacagagaattgagtgagatcgctgaacaggcaaagagaagggctgagattgcaagacttagggaattgcatacactcaaaggacacgtggagtcagttgttaagttgaagggtttagatatagagacaccaagtcactacacagtttga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z