pIGEM010
type: ComponentDefinition
persistent identity: BBa K1537010
display id: BBa_K1537010
version: 1
was generated by: iGEM to SBOL conversion
description: piGEM002+TCP02-HPS-PHI+Tp-AtFDH+FALDH
created: 2014-10-15T11:00:00Z
modified: 2015-05-08T01:10:52Z
mutable provenance: none.
owned by: james
owned by: myers
mutable description: none.
discontinued: false
dominant: false
experience: experience/None
group u list: _1919_
m user id: 0
owner id: 22415
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none.
star: false
creator: Jie Li
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K1537027
component: BBa_K1537019
component: BBa_K1537030
component: BBa_K1537031
component: BBa_K1537029
component: BBa_K1537020
component: BBa_K1537028
component: BBa_K1537025
component: BBa_K1537023
component: BBa_K1537026
component: BBa_K1537017
component: BBa_K1537022
component: BBa_K1537015
component: BBa_K1537016
component: BBa_K1537024
sequence annotation: BBa_K1537015
sequence annotation: BBa_K1537022
sequence annotation: BBa_K1537024
sequence annotation: BBa_K1537016
sequence annotation: BBa_K1537023
sequence annotation: BBa_K1537025
sequence annotation: BBa_K1537017
sequence annotation: BBa_K1537026
sequence annotation: BBa_K1537029
sequence annotation: BBa_K1537031
sequence annotation: BBa_K1537028
sequence annotation: BBa_K1537020
sequence annotation: BBa_K1537019
sequence annotation: BBa_K1537027
sequence annotation: BBa_K1537030
sequence: https://synbiohub.org/public/igem/BBa_K1537010_sequence/1

BBa_K1537027
type: Component
persistent identity: component2421220
display id: component2421220
version: 1
top level: pIGEM010
definition: BBa_K1537027
access: public

BBa_K1537019
type: Component
persistent identity: component2421218
display id: component2421218
version: 1
top level: pIGEM010
definition: BBa_K1537019
access: public

BBa_K1537030
type: Component
persistent identity: component2421222
display id: component2421222
version: 1
top level: pIGEM010
definition: BBa_K1537030
access: public

BBa_K1537031
type: Component
persistent identity: component2421212
display id: component2421212
version: 1
top level: pIGEM010
definition: BBa_K1537031
access: public

BBa_K1537029
type: Component
persistent identity: component2421210
display id: component2421210
version: 1
top level: pIGEM010
definition: BBa_K1537029
access: public

BBa_K1537020
type: Component
persistent identity: component2421216
display id: component2421216
version: 1
top level: pIGEM010
definition: BBa_K1537020
access: public

BBa_K1537028
type: Component
persistent identity: component2421214
display id: component2421214
version: 1
top level: pIGEM010
definition: BBa_K1537028
access: public

BBa_K1537025
type: Component
persistent identity: component2421203
display id: component2421203
version: 1
top level: pIGEM010
definition: BBa_K1537025
access: public

BBa_K1537023
type: Component
persistent identity: component2421201
display id: component2421201
version: 1
top level: pIGEM010
definition: BBa_K1537023
access: public

BBa_K1537026
type: Component
persistent identity: component2421208
display id: component2421208
version: 1
top level: pIGEM010
definition: BBa_K1537026
access: public

BBa_K1537017
type: Component
persistent identity: component2421206
display id: component2421206
version: 1
top level: pIGEM010
definition: BBa_K1537017
access: public

BBa_K1537022
type: Component
persistent identity: component2421193
display id: component2421193
version: 1
top level: pIGEM010
definition: BBa_K1537022
access: public

BBa_K1537015
type: Component
persistent identity: component2421191
display id: component2421191
version: 1
top level: pIGEM010
definition: BBa_K1537015
access: public

BBa_K1537016
type: Component
persistent identity: component2421199
display id: component2421199
version: 1
top level: pIGEM010
definition: BBa_K1537016
access: public

BBa_K1537024
type: Component
persistent identity: component2421196
display id: component2421196
version: 1
top level: pIGEM010
definition: BBa_K1537024
access: public

BBa_K1537015
type: SequenceAnnotation
persistent identity: annotation2421191
display id: annotation2421191
version: 1
top level: pIGEM010
location: https://synbiohub.org/public/igem/BBa_K1537010/annotation2421191/range2421191/1
component: BBa_K1537015

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persistent identity: range2421191
display id: range2421191
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top level: pIGEM010
direction: direction/reverse
start: 1
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orientation: inline

BBa_K1537022
type: SequenceAnnotation
persistent identity: annotation2421193
display id: annotation2421193
version: 1
top level: pIGEM010
location: https://synbiohub.org/public/igem/BBa_K1537010/annotation2421193/range2421193/1
component: BBa_K1537022

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type: Range
persistent identity: range2421193
display id: range2421193
version: 1
top level: pIGEM010
direction: direction/reverse
start: 867
end: 1037
orientation: inline

BBa_K1537024
type: SequenceAnnotation
persistent identity: annotation2421196
display id: annotation2421196
version: 1
top level: pIGEM010
location: https://synbiohub.org/public/igem/BBa_K1537010/annotation2421196/range2421196/1
component: BBa_K1537024

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type: Range
persistent identity: range2421196
display id: range2421196
version: 1
top level: pIGEM010
direction: direction/reverse
start: 1044
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orientation: inline

BBa_K1537016
type: SequenceAnnotation
persistent identity: annotation2421199
display id: annotation2421199
version: 1
top level: pIGEM010
location: https://synbiohub.org/public/igem/BBa_K1537010/annotation2421199/range2421199/1
component: BBa_K1537016

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persistent identity: range2421199
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top level: pIGEM010
direction: direction/reverse
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BBa_K1537023
type: SequenceAnnotation
persistent identity: annotation2421201
display id: annotation2421201
version: 1
top level: pIGEM010
location: https://synbiohub.org/public/igem/BBa_K1537010/annotation2421201/range2421201/1
component: BBa_K1537023

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persistent identity: range2421201
display id: range2421201
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top level: pIGEM010
direction: direction/reverse
start: 2345
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BBa_K1537025
type: SequenceAnnotation
persistent identity: annotation2421203
display id: annotation2421203
version: 1
top level: pIGEM010
location: https://synbiohub.org/public/igem/BBa_K1537010/annotation2421203/range2421203/1
component: BBa_K1537025

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type: Range
persistent identity: range2421203
display id: range2421203
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top level: pIGEM010
direction: direction/reverse
start: 2441
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BBa_K1537017
type: SequenceAnnotation
persistent identity: annotation2421206
display id: annotation2421206
version: 1
top level: pIGEM010
location: https://synbiohub.org/public/igem/BBa_K1537010/annotation2421206/range2421206/1
component: BBa_K1537017

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type: Range
persistent identity: range2421206
display id: range2421206
version: 1
top level: pIGEM010
direction: direction/reverse
start: 3514
end: 3576
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BBa_K1537026
type: SequenceAnnotation
persistent identity: annotation2421208
display id: annotation2421208
version: 1
top level: pIGEM010
location: https://synbiohub.org/public/igem/BBa_K1537010/annotation2421208/range2421208/1
component: BBa_K1537026

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type: Range
persistent identity: range2421208
display id: range2421208
version: 1
top level: pIGEM010
direction: direction/reverse
start: 3583
end: 4713
orientation: inline

BBa_K1537029
type: SequenceAnnotation
persistent identity: annotation2421210
display id: annotation2421210
version: 1
top level: pIGEM010
location: https://synbiohub.org/public/igem/BBa_K1537010/annotation2421210/range2421210/1
component: BBa_K1537029

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type: Range
persistent identity: range2421210
display id: range2421210
version: 1
top level: pIGEM010
direction: direction/reverse
start: 4722
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orientation: inline

BBa_K1537031
type: SequenceAnnotation
persistent identity: annotation2421212
display id: annotation2421212
version: 1
top level: pIGEM010
location: https://synbiohub.org/public/igem/BBa_K1537010/annotation2421212/range2421212/1
component: BBa_K1537031

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type: Range
persistent identity: range2421212
display id: range2421212
version: 1
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direction: direction/reverse
start: 4980
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BBa_K1537028
type: SequenceAnnotation
persistent identity: annotation2421214
display id: annotation2421214
version: 1
top level: pIGEM010
location: https://synbiohub.org/public/igem/BBa_K1537010/annotation2421214/range2421214/1
component: BBa_K1537028

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type: Range
persistent identity: range2421214
display id: range2421214
version: 1
top level: pIGEM010
direction: direction/reverse
start: 5239
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orientation: inline

BBa_K1537020
type: SequenceAnnotation
persistent identity: annotation2421216
display id: annotation2421216
version: 1
top level: pIGEM010
location: https://synbiohub.org/public/igem/BBa_K1537010/annotation2421216/range2421216/1
component: BBa_K1537020

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type: Range
persistent identity: range2421216
display id: range2421216
version: 1
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direction: direction/reverse
start: 8094
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BBa_K1537019
type: SequenceAnnotation
persistent identity: annotation2421218
display id: annotation2421218
version: 1
top level: pIGEM010
location: https://synbiohub.org/public/igem/BBa_K1537010/annotation2421218/range2421218/1
component: BBa_K1537019

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type: Range
persistent identity: range2421218
display id: range2421218
version: 1
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direction: direction/reverse
start: 9804
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BBa_K1537027
type: SequenceAnnotation
persistent identity: annotation2421220
display id: annotation2421220
version: 1
top level: pIGEM010
location: https://synbiohub.org/public/igem/BBa_K1537010/annotation2421220/range2421220/1
component: BBa_K1537027

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type: Range
persistent identity: range2421220
display id: range2421220
version: 1
top level: pIGEM010
direction: direction/reverse
start: 10674
end: 11756
orientation: inline

BBa_K1537030
type: SequenceAnnotation
persistent identity: annotation2421222
display id: annotation2421222
version: 1
top level: pIGEM010
location: https://synbiohub.org/public/igem/BBa_K1537010/annotation2421222/range2421222/1
component: BBa_K1537030

https://synbiohub.org/public/igem/BBa_K1537010/annotation2421222/range2421222/1
type: Range
persistent identity: range2421222
display id: range2421222
version: 1
top level: pIGEM010
direction: direction/reverse
start: 11765
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BBa_K1537015
type: ComponentDefinition
persistent identity: BBa K1537015
display id: BBa_K1537015
version: 1
was generated by: iGEM to SBOL conversion
description: 35S promoter+translation initiation optimized sequence for dicot+Mass translation enhancer
created: 2014-08-17T11:00:00Z
modified: 2015-05-08T01:10:52Z
mutable provenance: The 35S promoter has a large area of application in genetically modified plants.The translation initiation optimized sequence for dicot is from the paper "translation initiation optimized sequence for dicot"(Nucleic Acids Research Volume 36, Issue 3Pp. 861-871 So Nakagawa1, Yoshihito Niimura2, Takashi Gojobori3,4, Hiroshi Tanaka1,2,* and Kin-ichiro Miura5)
owned by: james
owned by: myers
mutable description: The 35S promoter is a strong promoter derived from cauliflower mosaic virus. This constitutive promoter is widely used in transgenic plants to improve the level of the expression of foreign genes effectively."AAAAAAATG" was a kind of preferred nucleotide sequence used in dicot plants（ATG is the initiation codon）to enhance translation initiation.And insertion of ???GCT TCC TCC???(mass translation enhancer) after the initiator codon ATG can augment downstream gene-expression in plants.
discontinued: false
dominant: false
experience: experience/None
group u list: _1919_
m user id: 0
owner id: 22415
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: We have to remove some restriction endonuclease cutting sites by changing a few bases.
star: false
creator: Jie Li
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: 35s promoter
sequence annotation: translation initiation optimized sequence for dicot
sequence annotation: mass translation enhancer
sequence: https://synbiohub.org/public/igem/BBa_K1537015_sequence/1

35s promoter
type: SequenceAnnotation
persistent identity: annotation2393797
display id: annotation2393797
version: 1
top level: BBa_K1537015
location: https://synbiohub.org/public/igem/BBa_K1537015/annotation2393797/range2393797/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K1537015/annotation2393797/range2393797/1
type: Range
persistent identity: range2393797
display id: range2393797
version: 1
top level: BBa_K1537015
direction: direction/forward
start: 1
end: 842
orientation: inline

translation initiation optimized sequence for dicot
type: SequenceAnnotation
persistent identity: annotation2393798
display id: annotation2393798
version: 1
top level: BBa_K1537015
location: https://synbiohub.org/public/igem/BBa_K1537015/annotation2393798/range2393798/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1537015/annotation2393798/range2393798/1
type: Range
persistent identity: range2393798
display id: range2393798
version: 1
top level: BBa_K1537015
direction: direction/forward
start: 843
end: 851
orientation: inline

mass translation enhancer
type: SequenceAnnotation
persistent identity: annotation2393799
display id: annotation2393799
version: 1
top level: BBa_K1537015
location: https://synbiohub.org/public/igem/BBa_K1537015/annotation2393799/range2393799/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K1537015/annotation2393799/range2393799/1
type: Range
persistent identity: range2393799
display id: range2393799
version: 1
top level: BBa_K1537015
direction: direction/forward
start: 852
end: 860
orientation: inline

BBa_K1537016
type: ComponentDefinition
persistent identity: BBa K1537016
display id: BBa_K1537016
version: 1
was generated by: iGEM to SBOL conversion
description: GSG linker+P2A
created: 2014-09-13T11:00:00Z
modified: 2015-05-08T01:10:52Z
mutable provenance: The part is from porcine teschovirus-1.
owned by: james
owned by: myers
mutable description: The 18~22 amino acids 2A self-cleaving oligopeptides can be used for co-expression of multiple, discrete proteins from a single ORF.Based on highly inefficient peptide bond formation between glycine and proline residues within the 2A peptide, placement of 2A peptide sequence as a linker region between tandem cDNA???s allows the stoichiometric translation of multiple unfused protein products. These sequences were first discovered in the foot-and-mouth disease virus (FMDV).And since than many 2A-like sequences have been identified in other viruses and some parasites.To minimize the risk of homologous recombination, it is important to use different 2A peptide sequences if more than two genes are being linked. The 2A peptide system has thus far worked successfully in all eukaryotic systems tested, from mammalian cells, yeast, and plants.In our project,we use F2A(from foot-and-mouth disease virus), P2A(from porcine teschovirus-1) and T2A(from Thosea asigna virus) to achieve our goal.And "GSG linker" is an oligopeptide of ???Gly-Ser-Gly??? between your protein and 2A peptide to enhance cleavage.
discontinued: false
dominant: false
experience: experience/None
group u list: _1919_
m user id: 0
owner id: 22415
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: We have to use this part by synthesis from biological company because the virus is dangerous.
star: false
creator: Jie Li
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: GSG linker
sequence annotation: P2A
sequence: https://synbiohub.org/public/igem/BBa_K1537016_sequence/1

GSG linker
type: SequenceAnnotation
persistent identity: annotation2383426
display id: annotation2383426
version: 1
top level: BBa_K1537016
location: https://synbiohub.org/public/igem/BBa_K1537016/annotation2383426/range2383426/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1537016/annotation2383426/range2383426/1
type: Range
persistent identity: range2383426
display id: range2383426
version: 1
top level: BBa_K1537016
direction: direction/forward
start: 1
end: 9
orientation: inline

P2A
type: SequenceAnnotation
persistent identity: annotation2383427
display id: annotation2383427
version: 1
top level: BBa_K1537016
location: https://synbiohub.org/public/igem/BBa_K1537016/annotation2383427/range2383427/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1537016/annotation2383427/range2383427/1
type: Range
persistent identity: range2383427
display id: range2383427
version: 1
top level: BBa_K1537016
direction: direction/forward
start: 10
end: 66
orientation: inline

BBa_K1537022
type: ComponentDefinition
persistent identity: BBa K1537022
display id: BBa_K1537022
version: 1
was generated by: iGEM to SBOL conversion
description: TCP02
created: 2014-09-16T11:00:00Z
modified: 2015-05-08T01:10:52Z
mutable provenance: Solanum lycopersicum.
owned by: james
owned by: myers
mutable description: Chloroplast transit peptides.
discontinued: false
dominant: false
group u list: _1919_
m user id: 0
owner id: 22415
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: We have to remove some restriction endonuclease cutting sites by changing a few bases.
star: false
creator: Jie Li
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TCP02
sequence: https://synbiohub.org/public/igem/BBa_K1537022_sequence/1

TCP02
type: SequenceAnnotation
persistent identity: annotation2417604
display id: annotation2417604
version: 1
top level: BBa_K1537022
location: https://synbiohub.org/public/igem/BBa_K1537022/annotation2417604/range2417604/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K1537022/annotation2417604/range2417604/1
type: Range
persistent identity: range2417604
display id: range2417604
version: 1
top level: BBa_K1537022
direction: direction/forward
start: 1
end: 171
orientation: inline

BBa_K1537024
type: ComponentDefinition
persistent identity: BBa K1537024
display id: BBa_K1537024
version: 1
was generated by: iGEM to SBOL conversion
description: HPS-PHI
created: 2014-09-16T11:00:00Z
modified: 2015-05-08T01:10:52Z
mutable provenance: Mycobacterium gastri MB19.
owned by: james
owned by: myers
mutable description: The ribulose monophosphate (RuMP) pathway is one of the HCHO-fixation pathways found in microorganisms called methylotrophs, which utilize one-carbon compounds as the sole carbon source. The key enzymes of this pathway are 3-hexulose-6-phosphate synthase (HPS), which fixes HCHO to D-ribulose-5-phosphate (Ru5P) to produce D-arabino-3-hexulose 6-phosphate (Hu6P), and 6-phospho-3- hexuloisomerase (PHI), which converts Hu6P to fructose 6-phosphate (F6P).The two key enzymes work in chloroplast both.We will use fusion expression to conduct heterologous expression in tobacco.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1919_
m user id: 0
owner id: 22415
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: We use HPS&PHI as co-expression.
star: false
creator: Jie Li
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: HPS
sequence annotation: PHI
sequence: https://synbiohub.org/public/igem/BBa_K1537024_sequence/1

HPS
type: SequenceAnnotation
persistent identity: annotation2383836
display id: annotation2383836
version: 1
top level: BBa_K1537024
location: https://synbiohub.org/public/igem/BBa_K1537024/annotation2383836/range2383836/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K1537024/annotation2383836/range2383836/1
type: Range
persistent identity: range2383836
display id: range2383836
version: 1
top level: BBa_K1537024
direction: direction/forward
start: 1
end: 621
orientation: inline

PHI
type: SequenceAnnotation
persistent identity: annotation2383837
display id: annotation2383837
version: 1
top level: BBa_K1537024
location: https://synbiohub.org/public/igem/BBa_K1537024/annotation2383837/range2383837/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K1537024/annotation2383837/range2383837/1
type: Range
persistent identity: range2383837
display id: range2383837
version: 1
top level: BBa_K1537024
direction: direction/forward
start: 622
end: 1221
orientation: inline

BBa_K1537017
type: ComponentDefinition
persistent identity: BBa K1537017
display id: BBa_K1537017
version: 1
was generated by: iGEM to SBOL conversion
description: GSG linker+T2A
created: 2014-09-15T11:00:00Z
modified: 2015-05-08T01:10:52Z
mutable provenance: T2A is from Thosea asigna virus.
owned by: james
owned by: myers
mutable description: The 18~22 amino acids 2A self-cleaving oligopeptides can be used for co-expression of multiple, discrete proteins from a single ORF.Based on highly inefficient peptide bond formation between glycine and proline residues within the 2A peptide, placement of 2A peptide sequence as a linker region between tandem cDNA???s allows the stoichiometric translation of multiple unfused protein products. These sequences were first discovered in the foot-and-mouth disease virus (FMDV).And since than many 2A-like sequences have been identified in other viruses and some parasites.To minimize the risk of homologous recombination, it is important to use different 2A peptide sequences if more than two genes are being linked. The 2A peptide system has thus far worked successfully in all eukaryotic systems tested, from mammalian cells, yeast, and plants.In our project,we use F2A(from foot-and-mouth disease virus), P2A(from porcine teschovirus-1) and T2A(from Thosea asigna virus) to achieve our goal.And GSG linker is an oligopeptide of ???Gly-Ser-Gly??? between your protein and 2A peptide to enhance cleavage.
discontinued: false
dominant: false
experience: experience/None
group u list: _1919_
m user id: 0
owner id: 22415
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: We have to use it by chemosynthesis because the virus is dangerous.
star: false
creator: Jie Li
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: GSG linker
sequence annotation: T2A
sequence: https://synbiohub.org/public/igem/BBa_K1537017_sequence/1

GSG linker
type: SequenceAnnotation
persistent identity: annotation2383712
display id: annotation2383712
version: 1
top level: BBa_K1537017
location: https://synbiohub.org/public/igem/BBa_K1537017/annotation2383712/range2383712/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1537017/annotation2383712/range2383712/1
type: Range
persistent identity: range2383712
display id: range2383712
version: 1
top level: BBa_K1537017
direction: direction/forward
start: 1
end: 9
orientation: inline

T2A
type: SequenceAnnotation
persistent identity: annotation2383711
display id: annotation2383711
version: 1
top level: BBa_K1537017
location: https://synbiohub.org/public/igem/BBa_K1537017/annotation2383711/range2383711/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1537017/annotation2383711/range2383711/1
type: Range
persistent identity: range2383711
display id: range2383711
version: 1
top level: BBa_K1537017
direction: direction/forward
start: 10
end: 63
orientation: inline

BBa_K1537019
type: ComponentDefinition
persistent identity: BBa K1537019
display id: BBa_K1537019
version: 1
was generated by: iGEM to SBOL conversion
description: TA29 promoter
created: 2014-09-15T11:00:00Z
modified: 2015-05-08T01:10:52Z
mutable provenance: tissue specific gene of tobacco.
owned by: james
owned by: myers
mutable description: TA29 promoter is a tissue-specific(tapetal cells) promoter found in tobacco.
discontinued: false
dominant: false
group u list: _1919_
m user id: 0
owner id: 22415
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: no
star: false
creator: Jie Li
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: TA29 promoter
sequence: https://synbiohub.org/public/igem/BBa_K1537019_sequence/1

TA29 promoter
type: SequenceAnnotation
persistent identity: annotation2417290
display id: annotation2417290
version: 1
top level: BBa_K1537019
location: https://synbiohub.org/public/igem/BBa_K1537019/annotation2417290/range2417290/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K1537019/annotation2417290/range2417290/1
type: Range
persistent identity: range2417290
display id: range2417290
version: 1
top level: BBa_K1537019
direction: direction/forward
start: 1
end: 864
orientation: inline

BBa_K1537020
type: ComponentDefinition
persistent identity: BBa K1537020
display id: BBa_K1537020
version: 1
was generated by: iGEM to SBOL conversion
description: GC1 promoter
created: 2014-09-16T11:00:00Z
modified: 2015-05-08T01:10:52Z
mutable provenance: Arabidopsis.
owned by: james
owned by: myers
mutable description: The GC1 promoter drives strong reporter expression in guard cells of Arabidopsis and tobacco plants. It provides a potent research tool for targeted guard cell expression.
discontinued: false
dominant: false
group u list: _1919_
m user id: 0
owner id: 22415
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: no
star: false
creator: Jie Li
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: GC1 promoter
sequence: https://synbiohub.org/public/igem/BBa_K1537020_sequence/1

GC1 promoter
type: SequenceAnnotation
persistent identity: annotation2417291
display id: annotation2417291
version: 1
top level: BBa_K1537020
location: https://synbiohub.org/public/igem/BBa_K1537020/annotation2417291/range2417291/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K1537020/annotation2417291/range2417291/1
type: Range
persistent identity: range2417291
display id: range2417291
version: 1
top level: BBa_K1537020
direction: direction/forward
start: 1
end: 1702
orientation: inline

BBa_K1537023
type: ComponentDefinition
persistent identity: BBa K1537023
display id: BBa_K1537023
version: 1
was generated by: iGEM to SBOL conversion
description: TCP03
created: 2014-09-16T11:00:00Z
modified: 2015-05-08T01:10:52Z
mutable provenance: Arabidopsis thaliana
owned by: james
owned by: myers
mutable description: Leading formate dehydrogenase into chloroplast.
discontinued: false
dominant: false
group u list: _1919_
m user id: 0
owner id: 22415
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Jie Li
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TCP03
sequence: https://synbiohub.org/public/igem/BBa_K1537023_sequence/1

TCP03
type: SequenceAnnotation
persistent identity: annotation2417605
display id: annotation2417605
version: 1
top level: BBa_K1537023
location: https://synbiohub.org/public/igem/BBa_K1537023/annotation2417605/range2417605/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1537023/annotation2417605/range2417605/1
type: Range
persistent identity: range2417605
display id: range2417605
version: 1
top level: BBa_K1537023
direction: direction/forward
start: 1
end: 90
orientation: inline

BBa_K1537025
type: ComponentDefinition
persistent identity: BBa K1537025
display id: BBa_K1537025
version: 1
was generated by: iGEM to SBOL conversion
description: FDH
created: 2014-09-16T11:00:00Z
modified: 2015-05-08T01:10:52Z
mutable provenance: Arabidopsis thaliana.
owned by: james
owned by: myers
mutable description: Formate dehydrogenase is a mitochondrial-localized NAD-requiring enzyme while the HCOOH is getting into the mitochondrial，FDH will oxidize the formic acid into CO2, and reduce NAD+ to NADH with a high degree of specificity.In our project, the heterologous expression of FDH from arabidopsis thaliana in tobacco was completed.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1919_
m user id: 0
owner id: 22415
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: no
star: false
creator: Jie Li
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: FDH
sequence: https://synbiohub.org/public/igem/BBa_K1537025_sequence/1

FDH
type: SequenceAnnotation
persistent identity: annotation2417287
display id: annotation2417287
version: 1
top level: BBa_K1537025
location: https://synbiohub.org/public/igem/BBa_K1537025/annotation2417287/range2417287/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K1537025/annotation2417287/range2417287/1
type: Range
persistent identity: range2417287
display id: range2417287
version: 1
top level: BBa_K1537025
direction: direction/forward
start: 1
end: 1065
orientation: inline

BBa_K1537026
type: ComponentDefinition
persistent identity: BBa K1537026
display id: BBa_K1537026
version: 1
was generated by: iGEM to SBOL conversion
description: FALDH
created: 2014-09-16T11:00:00Z
modified: 2015-05-08T01:10:52Z
mutable provenance: Brevibacillus brevis
owned by: james
owned by: myers
mutable description: The glutathione-dependent formaldehyde dehydrogenase(FALDH) plays a key role in formaldehyde metabolism. FALDH is identified as an enzyme expressed in the cytoplasm. If we make FALDH over-express in plants, we can enhance plants??? tolerance to HCHO and increase the ability of plants to absorb HCHO. In the process of metabolism of formaldehyde, the formaldehyde may first combined with glutathione (GSH) to form the product of S-hydroxymethyl glutathione (HM-GSH), then FALDH in cytoplasm will catalyzes the formation of a S-formyl glutathione(F-GSH). Next the F-GSH will be hydrolyzed to formate(HCOOH) and GSH by S-formyl glutathione hydrolase (FGH).
discontinued: false
dominant: false
experience: experience/Works
group u list: _1919_
m user id: 0
owner id: 22415
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: none
star: false
creator: Jie Li
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: FALDH
sequence: https://synbiohub.org/public/igem/BBa_K1537026_sequence/1

FALDH
type: SequenceAnnotation
persistent identity: annotation2417288
display id: annotation2417288
version: 1
top level: BBa_K1537026
location: https://synbiohub.org/public/igem/BBa_K1537026/annotation2417288/range2417288/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1537026/annotation2417288/range2417288/1
type: Range
persistent identity: range2417288
display id: range2417288
version: 1
top level: BBa_K1537026
direction: direction/forward
start: 1
end: 1131
orientation: inline

BBa_K1537027
type: ComponentDefinition
persistent identity: BBa K1537027
display id: BBa_K1537027
version: 1
was generated by: iGEM to SBOL conversion
description: AdCP
created: 2014-09-16T11:00:00Z
modified: 2015-05-08T01:10:52Z
mutable provenance: Arachis diogoi
owned by: james
owned by: myers
mutable description: ATGGCTAGGCTCTCTTTATTGTTGTTACTTCTCGTGGCAGCTGTGGCTACCGCAGTTGATGATCAGGCAGATCCTCTCATTAGGCAGGTTACTGATGGAGATCATCACATGCTTAATGCTGAACATCACTTCACTACATTCAAGACTAAGTTCGGAAAGTCTTATGCTACACAAGAAGAGCATGATTACAGATTTGGTGTTTTCAGGGCAAACCTCAGAAGGGCTAAACTTCATGCAAAACTTGATCCTAGTGCTGAGCATGGAGTTACAAAGTTTTCTGATCTTACCCCAGAAGAGTTCAAGAGACAATATCTCGGTTTAAAACCTCTTAGGTTGCCATCAACCGCTAATAAGGCACCTATTCTCCCTACTTCTGATCTTCCTGAAAACTTTGATTGGAGAGATAAGGGAGCTGTTACTCCAGTGAAAAATCAAGGAAGTTGTGGTTCTTGCTGGGCTTTCAGTACCACTGGTGCTCTTGAAGGTGCACATTACTTGTCAACAGGTGAACTCGTTTCTTTATCAGAGCAACAGTTGGTTGATTGTGATCACGTGTGCGATCCTGAAGAGTATGGAGCTTGTGATGCAGGTTGCAACGGAGGTCTTATGAATAACGCTTTCGATTACATTTTGCAAGCAGGAGGTGTGCAGACCGAAAAGGATTATCCATACTCTGGAAGAGATGAGACATGTAAATTTGATAAGTCAAAGGTTGCTGCAACCGTGGCTAACTTCTCAGTTGTGAGTCTCGATGAAGATCAAATCGCTGCAAATCTCGTTAAGCATGGACCTTTAGCTGTGGGAATTAACGCAATTTTCATGCAGACATACATCGGAGGTGTTTCTTGTCCATACATATGCGGAAAAAACTTGGATCACGGTGTTCTTTTGGTGGGATATGGTGCTGCTGGATACGCTCCTATTAGATTCAAGGATAAGCCATTCTGGATTATCAAAAATTCTTGGGGAGAATCATGGGGAGAGGATGGTTATTATAAGATTTGTAGAGGAAAGAATGTTTGTGGTGTGGATTCAATGGTTTCATCTGTGGTTGCTACTACTTTCACTTCATCAAATAACTGA
discontinued: false
dominant: false
experience: experience/None
group u list: _1919_
m user id: 0
owner id: 22415
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: We have to remove some restriction endonuclease cutting sites by changing a few bases.
star: false
creator: Jie Li
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: AdCP
sequence: https://synbiohub.org/public/igem/BBa_K1537027_sequence/1

AdCP
type: SequenceAnnotation
persistent identity: annotation2417286
display id: annotation2417286
version: 1
top level: BBa_K1537027
location: https://synbiohub.org/public/igem/BBa_K1537027/annotation2417286/range2417286/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1537027/annotation2417286/range2417286/1
type: Range
persistent identity: range2417286
display id: range2417286
version: 1
top level: BBa_K1537027
direction: direction/forward
start: 1
end: 1083
orientation: inline

BBa_K1537028
type: ComponentDefinition
persistent identity: BBa K1537028
display id: BBa_K1537028
version: 1
was generated by: iGEM to SBOL conversion
description: AHA2
created: 2014-09-17T11:00:00Z
modified: 2015-05-08T01:10:52Z
mutable provenance: Arabidopsis thaliana
owned by: james
owned by: myers
mutable description: AHA2 belongs to the P-type ATPase superfamily of cation-transporting ATPases, pumps protons out of the cell, generating a proton gradient that drives the active transport of nutrients by proton symport.We want to enlarge the tobacco AHA2 to
discontinued: false
dominant: false
experience: experience/None
group u list: _1919_
m user id: 0
owner id: 22415
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: We have to remove some restriction endonuclease cutting sites by changing a few bases.
star: false
creator: Jie Li
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: AHA2
sequence: https://synbiohub.org/public/igem/BBa_K1537028_sequence/1

AHA2
type: SequenceAnnotation
persistent identity: annotation2417289
display id: annotation2417289
version: 1
top level: BBa_K1537028
location: https://synbiohub.org/public/igem/BBa_K1537028/annotation2417289/range2417289/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K1537028/annotation2417289/range2417289/1
type: Range
persistent identity: range2417289
display id: range2417289
version: 1
top level: BBa_K1537028
direction: direction/forward
start: 1
end: 2847
orientation: inline

BBa_K1537029
type: ComponentDefinition
persistent identity: BBa K1537029
display id: BBa_K1537029
version: 1
was generated by: iGEM to SBOL conversion
description: HSP terminator
created: 2014-09-17T11:00:00Z
modified: 2015-05-08T01:10:52Z
mutable provenance: Arabidopsis thaliana
owned by: james
owned by: myers
mutable description: The heat shock protein ( HSP ) terminator increases mRNA levels of both transiently and stably expressed transgenes in plants effectively.
discontinued: false
dominant: false
group u list: _1919_
m user id: 0
owner id: 22415
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none.
star: false
creator: Jie Li
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: HSP terminator
sequence: https://synbiohub.org/public/igem/BBa_K1537029_sequence/1

HSP terminator
type: SequenceAnnotation
persistent identity: annotation2417608
display id: annotation2417608
version: 1
top level: BBa_K1537029
location: https://synbiohub.org/public/igem/BBa_K1537029/annotation2417608/range2417608/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K1537029/annotation2417608/range2417608/1
type: Range
persistent identity: range2417608
display id: range2417608
version: 1
top level: BBa_K1537029
direction: direction/forward
start: 1
end: 250
orientation: inline

BBa_K1537030
type: ComponentDefinition
persistent identity: BBa K1537030
display id: BBa_K1537030
version: 1
was generated by: iGEM to SBOL conversion
description: CaMV35S polyA
created: 2014-09-17T11:00:00Z
modified: 2015-05-08T01:10:52Z
mutable provenance: Cauliflower mosaic virus
owned by: james
owned by: myers
mutable description: It???s a kind of terminator derived from cauliflower mosaic virus.
discontinued: false
dominant: false
group u list: _1919_
m user id: 0
owner id: 22415
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Jie Li
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: CaMV35S polyA
sequence: https://synbiohub.org/public/igem/BBa_K1537030_sequence/1

CaMV35S polyA
type: SequenceAnnotation
persistent identity: annotation2417606
display id: annotation2417606
version: 1
top level: BBa_K1537030
location: https://synbiohub.org/public/igem/BBa_K1537030/annotation2417606/range2417606/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K1537030/annotation2417606/range2417606/1
type: Range
persistent identity: range2417606
display id: range2417606
version: 1
top level: BBa_K1537030
direction: direction/forward
start: 1
end: 216
orientation: inline

BBa_K1537031
type: ComponentDefinition
persistent identity: BBa K1537031
display id: BBa_K1537031
version: 1
was generated by: iGEM to SBOL conversion
description: NOS ( nopaline synthase ) terminator
created: 2014-09-17T11:00:00Z
modified: 2015-05-08T01:10:52Z
mutable provenance: Agrobacterium tumefasciens
owned by: james
owned by: myers
mutable description: It???s a quite common terminator in expression system of plants.
discontinued: false
dominant: false
group u list: _1919_
m user id: 0
owner id: 22415
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None.
star: false
creator: Jie Li
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: NOS terminator
sequence: https://synbiohub.org/public/igem/BBa_K1537031_sequence/1

NOS terminator
type: SequenceAnnotation
persistent identity: annotation2417607
display id: annotation2417607
version: 1
top level: BBa_K1537031
location: https://synbiohub.org/public/igem/BBa_K1537031/annotation2417607/range2417607/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K1537031/annotation2417607/range2417607/1
type: Range
persistent identity: range2417607
display id: range2417607
version: 1
top level: BBa_K1537031
direction: direction/forward
start: 1
end: 253
orientation: inline

https://synbiohub.org/public/igem/BBa_K1537010_sequence/1
type: Sequence
persistent identity: BBa K1537010 sequence
display id: BBa_K1537010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agatttgccttttcaatttcagaaagaatgctaacccacagatggttagagaggcttacgcagcaggtctcatcaagacgatctacccgagcaataatctccaggaaatcaaataccttcccaagaaggttaaagatgcagtcaaaagattcaggactaactgcatcaagaacacagagaaagatatatttctcaagatcagaagtactattccagtatggacgattcaaggcttgcttcacaaaccaaggcaagtaatagagattggagtctctaaaaaggtagttcccactgaatcaaaggccatggagtcaaagattcaaatagaggacctaacagaactcgccgtaaagactggcgaacagttcatacagagtctcttacgactcaatgacaagaagaaaatcttcgtcaacatggtggagcacgacacacttgtctactccaaaaatatcaaagatacagtctcagaagaccaaagggcaattgagacttttcaacaaagggtaatatccggaaacctcctcggattccattgcccagctatctgtcactttattgtgaagatagtggaaaaggaaggtggctcctacaaatgccatcattgcgataaaggaaaggccatcgttgaagatgcctctgccgacagtggtcccaaagatggacccccacccacgaggagcatcgtggaaaaagaagacgttccaaccacgtcttcaaagcaagtggattgatgtgatatctccactgacgtaagggatgacgcacaatcccactatccttcgcaagacccttcctctatataaggaagttcatttcatttggagagaacacgggggactaaaaaaatggcttcctcctactagatggcttcttcagtgatgtcatcagcagcagtcgcaacaagaggaaacggagcccaagcatcaatggtggcaccgtttacaggtttaaagagtacagcttcttttcctgtttcaagaaagcaaaatttggatataacctcaatcgctagtaacggaggaagggtgtcttgttactagatgaaacttcaggttgctatcgatcttttgtcaacagaagctgcattagagcttgctggaaaggtggcagaatatgttgatattatcgagttaggtactcctcttatagaagctgagggattgtcagtgattacagctgttaagaaagcacatccagataagatcgttttcgctgatatgaagactatggatgcaggtgaacttgaggctgatatagcattcaaagctggtgcagatttggtgacagttctcggaagtgctgatgattctaccattgctggagcagttaaggctgcacaagctcataataagggtgttgtggttgatttgattggtatagaagataaggctacaagagcacaagaggtgagggctctcggtgcaaaatttgttgaaatgcacgctggattagatgagcaggcaaagcctggattcgatcttaatggtcttcttgctgctggagaaaaggctagagtgcctttctctgttgcaggaggtgtgaaggttgctacaattccagcagttcaaaaagctggtgcagaggtggctgttgcaggaggtgctatctacggagctgctgatcctgctgctgctgctaaagaattgagagctgcaatagctatgactcaggctgctgaagctgatggtgcagttaaggtggttggagatgatattaccaataacttgtcactcgtgagggatgaagttgctgatactgctgcaaaagttgatcctgagcaggtggctgttcttgcaagacaaatcgttcagcctggtagggtgtttgttgctggagcaggtagatcaggattagttcttagaatggctgcaatgaggttaatgcatttcggtcttaccgtgcacgttgctggagatactacaacccctgctatatctgctggagatttgttgctcgttgcttctggatcaggtactacatcaggagtggttaaaagt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1537015_sequence/1
type: Sequence
persistent identity: BBa K1537015 sequence
display id: BBa_K1537015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agatttgccttttcaatttcagaaagaatgctaacccacagatggttagagaggcttacgcagcaggtctcatcaagacgatctacccgagcaataatctccaggaaatcaaataccttcccaagaaggttaaagatgcagtcaaaagattcaggactaactgcatcaagaacacagagaaagatatatttctcaagatcagaagtactattccagtatggacgattcaaggcttgcttcacaaaccaaggcaagtaatagagattggagtctctaaaaaggtagttcccactgaatcaaaggccatggagtcaaagattcaaatagaggacctaacagaactcgccgtaaagactggcgaacagttcatacagagtctcttacgactcaatgacaagaagaaaatcttcgtcaacatggtggagcacgacacacttgtctactccaaaaatatcaaagatacagtctcagaagaccaaagggcaattgagacttttcaacaaagggtaatatccggaaacctcctcggattccattgcccagctatctgtcactttattgtgaagatagtggaaaaggaaggtggctcctacaaatgccatcattgcgataaaggaaaggccatcgttgaagatgcctctgccgacagtggtcccaaagatggacccccacccacgaggagcatcgtggaaaaagaagacgttccaaccacgtcttcaaagcaagtggattgatgtgatatctccactgacgtaagggatgacgcacaatcccactatccttcgcaagacccttcctctatataaggaagttcatttcatttggagagaacacgggggactaaaaaaatggcttcctcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1537016_sequence/1
type: Sequence
persistent identity: BBa K1537016 sequence
display id: BBa_K1537016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggtagtggagcaacaaacttctcattattaaagcaagcaggagatgtggaggaaaatcctggtcca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1537017_sequence/1
type: Sequence
persistent identity: BBa K1537017 sequence
display id: BBa_K1537017_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggtagtggagagggtagaggatctcttttgacatgtggagatgttgaagagaaccctggacca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1537022_sequence/1
type: Sequence
persistent identity: BBa K1537022 sequence
display id: BBa_K1537022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcttcagtgatgtcatcagcagcagtcgcaacaagaggaaacggagcccaagcatcaatggtggcaccgtttacaggtttaaagagtacagcttcttttcctgtttcaagaaagcaaaatttggatataacctcaatcgctagtaacggaggaagggtgtcttgt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1537024_sequence/1
type: Sequence
persistent identity: BBa K1537024 sequence
display id: BBa_K1537024_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaacttcaggttgctatcgatcttttgtcaacagaagctgcattagagcttgctggaaaggtggcagaatatgttgatattatcgagttaggtactcctcttatagaagctgagggattgtcagtgattacagctgttaagaaagcacatccagataagatcgttttcgctgatatgaagactatggatgcaggtgaacttgaggctgatatagcattcaaagctggtgcagatttggtgacagttctcggaagtgctgatgattctaccattgctggagcagttaaggctgcacaagctcataataagggtgttgtggttgatttgattggtatagaagataaggctacaagagcacaagaggtgagggctctcggtgcaaaatttgttgaaatgcacgctggattagatgagcaggcaaagcctggattcgatcttaatggtcttcttgctgctggagaaaaggctagagtgcctttctctgttgcaggaggtgtgaaggttgctacaattccagcagttcaaaaagctggtgcagaggtggctgttgcaggaggtgctatctacggagctgctgatcctgctgctgctgctaaagaattgagagctgcaatagctatgactcaggctgctgaagctgatggtgcagttaaggtggttggagatgatattaccaataacttgtcactcgtgagggatgaagttgctgatactgctgcaaaagttgatcctgagcaggtggctgttcttgcaagacaaatcgttcagcctggtagggtgtttgttgctggagcaggtagatcaggattagttcttagaatggctgcaatgaggttaatgcatttcggtcttaccgtgcacgttgctggagatactacaacccctgctatatctgctggagatttgttgctcgttgcttctggatcaggtactacatcaggagtggttaaaagtgctgaaactgcaaagaaagctggagcaagaattgctgcttttactactaatcctgattctccattggctggactcgctgatgcagtggttataattccagctgcacaaaagactgatcatggttctcacatttcaaggcaatatgctggaagtttgtttgaacaggtgctcttcgtggttacagaggctgttttccagtctttatgggatcacacagaggtggaggctgaagaattatggacaagacacgcaaacttagagtga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1537019_sequence/1
type: Sequence
persistent identity: BBa K1537019 sequence
display id: BBa_K1537019_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttttggttagcgaatgcatttaatttagacattgtgttatgttccacttaaccgcttccctgcacttctttcaatctatctctcgatagaaaattgtgatactttgcgacttctatcagaggactttttgttttccatgtaacaatctgtcattttcgatggggagatttgcacaaataggctatttatgtgtcccaatttaaattttaaccccatgtcgatcagaacttagccacgagcaccagaagtttgatggatatgtgactttgtcactatccggtttactaatcaagagctatttttattcaaaattggatatctagctaagtataactggataatttgcattaacagattgaatatagtgccaaacaagaagggacaattgacttgtcactttatgaaagatgattcaaacatgattttttatgtactaatatatacatcctactcgaattaaagcgacataggctcgaagtatgcacatttagcaatgtaaattaaatcagtttttgaatcaagctaaaagcagacttgcataaggtgggtggctggactagaataaacatcttctctagcacagcttcataatgtaatttccataactgaaatcagggtgagacaaaattttggtactttttcctcacactaagtccatgtttgcaacaatttaatacatgaaaccttaatgttaccctcagattagcctgctactccccattttcctcgaaatgctccaacaaaagttagttttgcaagttgttgtgtatgtcttgtgctctatatatgcccttgtggtgcaagtgtaacagtacaacatcatcactcaaatcaaagtttttacttaaagaaattagctaaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1537020_sequence/1
type: Sequence
persistent identity: BBa K1537020 sequence
display id: BBa_K1537020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taacccgatgctcctgctcttcctcaagaccttccttgattcgccgccggtatgttctccgtctgtggtagcgcctttggaacactctaccaacgccgccatgaaaggatctctcatggccgcaggggacgtgttcttcttacatctggtgttagggctatggttactccagtgaggagggagaggcaagaggttgcttaatgattcgtttttccggtgatacgagaactctttaggtttaccgggaaggttttcccatgaaaatgggatgccaagtggatggagaggagttgccggagagttgccggagaataggagggaattggaggaggaggaagagagtgatcgccgggttgaaatcttaaccgtcgaggagaatttgaccgagttggatcgtctagtaggtacaattcgggtccttggcgaagtatccattcaaaatagtgtttagttttggacttgagaacttgttgtctctttgatctcttttatataaaactttggacgtgtaggacaaacttgtcaacataagaaacaaaatggttgcaacagagaggatgaatttataagttttcaacaccgcttttcttattagacggacaacaatctatagtggagtaaatttttatttttggtaaaatggttagtcaattcaaatatctaaattttgtgactcactaacattaacaaatatgcataagacataaaaaaaagaaagaataattcttatgaaacaagaaaaaaaacctatacaatcaatctttaggaattgacgatgtagaattgtagatgataaattttctcaaatatagatgggcctaatgaagggtgccgcttattggatctgacccattttgaggacaataatattttcattggttataagccttttaatcaaaattgtcattaaattgatgtctccctctcgggtcattttcctttctccctcacaaataatgtagactttagcaatttgctcgctgtgctttgtctttatatttagtaacacaaacattttgacttgtcttgtagagtttttctcttttatttttctatccaatatgaaaactaaaagtgttctcgtatacatatattaaaattaaagaaacctatgaaaacaccaatacaaatgcgatattgttttcagttcgacgtttcatgtttgttagaaaatttctaatgacgtttgtataaaatagacaattaaacgccaaacactacatctgtgttttcgaacaatattgcgtctgcgtttccttcatctatctctctcagtgtcacaatgtctgaactaagagacagctgtaaactatcattaagacataaactaccaaagtatcaagctaatgtaaaaattactctcatttccacgtaacaaattgagttagcttaacatattagtgaaactaggtttgaattttcttcttcttcttccatgcatcctccgaaaaaagggaaccaatcaaaactgtttgcatatcaaactccaacactttacagcaaatgcaatctataatctgtgatttatccaataaaaacctgtgatttatgtttggctccagcgatgaaagtctatgcatgtgatctctatccaacatgagtaattgttcagaaaataaaaagtagctgaaatgtatctatataaagaatcatccacaagtactattttcacacactacttcaaaatcactactcaagaaat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1537023_sequence/1
type: Sequence
persistent identity: BBa K1537023 sequence
display id: BBa_K1537023_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcaatgagacaggctgctaaggcaacaatcagggcatgtagtagtagttcttcaagtggttatttcgcaaggagacagttcaacgct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1537025_sequence/1
type: Sequence
persistent identity: BBa K1537025 sequence
display id: BBa_K1537025_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtcttcaggagatagtaagaaaattgttggtgtgttttataaggctaacgagtacgcaactaaaaatccaaacttccttggatgtgttgagaatgctcttggtattagagattggttggaatcacaaggacatcagtatatcgttacagatgataaggagggtccagattgcgaacttgagaaacatattcctgatctccacgttttaatctctacaccatttcatcctgcttacgtgaccgcagaaaggattaagaaagctaagaacttgaaacttttgctcaccgcaggaatcggttcagatcacattgatcttcaagctgctgctgctgctggacttactgttgctgaagtgactggtagtaacgttgtgtctgttgcagaagatgagttgatgagaattttgattcttatgaggaacttcgttcctggatataaccaagttgtgaagggagagtggaatgtggcaggtatcgcttatagagcatacgatcttgaaggaaaaaccataggtactgttggagctggtagaattggaaagttacttttgcagaggttgaaaccatttggttgtaatctcttataccatgataggctccaaatggctcctgaattagagaaggaaacaggtgcaaaattcgttgaggatcttaatgagatgttgccaaagtgcgatgttatagtgattaacatgcctctcacagagaaaaccagaggaatgttcaataaggaactcataggaaaattaaagaaaggtgttttgatcgtgaataacgctagaggagctattatggaaaggcaggctgttgtggatgcagttgaatcaggacacatcggaggttatagtggagatgtttgggaccctcaaccagctcctaaggatcatccatggagatacatgcctaaccaggctatgactccacacacatctggaactacaatcgatgctcaactcagatatgcagctggaactaaggatatgttagagaggtacttcaaaggtgaagattttccaaccgagaattacatagtgaaggatggtgaacttgctccacaatacagg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1537026_sequence/1
type: Sequence
persistent identity: BBa K1537026 sequence
display id: BBa_K1537026_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgggagcagtgacttaccaaggaaaaaattctatcgctgttaagaaagtggatgcaccatcaattcaggatagagaggatgttattatcaggatcacttctacagctatatgtggatctgatcttcatctttatcagggtaacttccctttgccaataggatacgttattggtcacgaacctatgggaattgttgaagaagtgggtccagatgttacagctgtgaagaaaggagatagagttgtgatcccttttaccgttgcatgtggtaaatgccaatattgtgatcatcatcttgaatcacagtgcgataatagtaacccacattatgatagtggaggtttattcggatactctgagaagtatggtaattaccctggaggtcaagctgaatatctcagggttccatttggaaactacacacctttcaaaattccagatgattgcgaattggaggatgaacagcttttgtttctctctgatgtgttacctaccgcttactggtctgttgagcatgcaggagtgaagaaaggagatacagttatcgtgctcggatgtggtccagttggacttatggctcaacagttcgcatggcaaaagggtgctgaaagagttattgcagtggattatatcgattacagacttaggcacgctaaaaggatgaatggtgttgaggtgtttgatttcactgaagatcctgatatgggtgagacacttaaagaattgaccaaaggaggtgcagatgttgtgattgattgtgttggtatggatggaaagaagtctcctttggagaagatcgaacaaaaacttaaacttcaaggaggtactataggtccaatccaaatagctacaaaggcagttagaaaaaggggaaccgttcagatgactggtgtgtatggaggtctttacaatatgtttcctcttggagctttctttgcaagaaacgttacacttaaaatgggacaagctccagcaaggggttatatgtcaaagttgtaccagaaagtgaccagtggagagattgatcctagagctataattactcacaaactcccattagatgaagctgcacacgcataccatatcttcaacgataaacaggatgattgcataaaagtgattctcaagcct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1537027_sequence/1
type: Sequence
persistent identity: BBa K1537027 sequence
display id: BBa_K1537027_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggctaggctctctttattgttgttacttctcgtggcagctgtggctaccgcagttgatgatcaggcagatcctctcattaggcaggttactgatggagatcatcacatgcttaatgctgaacatcacttcactacattcaagactaagttcggaaagtcttatgctacacaagaagagcatgattacagatttggtgttttcagggcaaacctcagaagggctaaacttcatgcaaaacttgatcctagtgctgagcatggagttacaaagttttctgatcttaccccagaagagttcaagagacaatatctcggtttaaaacctcttaggttgccatcaaccgctaataaggcacctattctccctacttctgatcttcctgaaaactttgattggagagataagggagctgttactccagtgaaaaatcaaggaagttgtggttcttgctgggctttcagtaccactggtgctcttgaaggtgcacattacttgtcaacaggtgaactcgtttctttatcagagcaacagttggttgattgtgatcacgtgtgcgatcctgaagagtatggagcttgtgatgcaggttgcaacggaggtcttatgaataacgctttcgattacattttgcaagcaggaggtgtgcagaccgaaaaggattatccatactctggaagagatgagacatgtaaatttgataagtcaaaggttgctgcaaccgtggctaacttctcagttgtgagtctcgatgaagatcaaatcgctgcaaatctcgttaagcatggacctttagctgtgggaattaacgcaattttcatgcagacatacatcggaggtgtttcttgtccatacatatgcggaaaaaacttggatcacggtgttcttttggtgggatatggtgctgctggatacgctcctattagattcaaggataagccattctggattatcaaaaattcttggggagaatcatggggagaggatggttattataagatttgtagaggaaagaatgtttgtggtgtggattcaatggtttcatctgtggttgctactactttcacttcatcaaataactga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1537028_sequence/1
type: Sequence
persistent identity: BBa K1537028 sequence
display id: BBa_K1537028_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtcatcattagaagatattaagaacgagactgtggatttggagaagatacctatagaggaagtgtttcagcaactcaagtgtagtagagagggtttgactacacaagaaggagaggataggatacagatttttggtccaaacaaacttgaagagaagaaagagtctaaacttttgaaatttcttggattcatgtggaatcctttgagttgggttatggaaatggctgcaatcatggctatagcacttgctaacggagatggtagacctccagattggcaagattttgtgggtattatctgtctcttagttatcaattcaactattagtttcatcgaagagaataacgctggaaacgctgctgctgctcttatggctggtcttgcaccaaagacaaaagttcttagagatggaaagtggtctgaacaagaggctgcaattctcgttcctggagatattgtgtcaattaaattaggagatattattccagcagatgctagacttttggagggagatcctttgaaggttgatcagtctgctcttacaggagagtcattgccagttaccaagcatcctggtcaagaagtgttcagtggatctacttgcaaacagggtgaaattgaggctgttgtgatcgcaaccggagttcatactttctttggaaaggctgcacacctcgttgattcaacaaatcaagtgggacactttcagaaagttcttaccgcaatcggtaacttctgtatttgctctatcgctattggaatggttattgaaatcatagtgatgtatccaatccaaagaaggaaatacagagatggtatagataacctcttagttcttttgattggaggtattccaatcgcaatgcctactgttttgtctgtgacaatggctattggaagtcatagactctctcaacagggtgctataacaaaaaggatgaccgcaattgaagagatggctggtatggatgttctttgttctgataagaccggaactctcacactcaacaaactttcagtggataagaacttggttgaggtgttttgcaagggagttgaaaaagatcaagtgctcttattcgctgcaatggcttcaagagttgaaaatcaggatgctatcgatgctgcaatggtgggaatgttggctgatccaaaagaggcaagagctggaattagggaggttcattttcttccattcaatcctgtggataagagaaccgctttgacttatatcgatggaagtggtaactggcacagggtttctaagggtgctcctgagcaaattcttgaattggcaaaggcttctaacgatctcagtaagaaagttttaagtattatcgataagtacgctgagagaggactcaggtctttagcagtggctagacaggttgtgccagagaagactaaagaatctcctggtgctccttgggagtttgttggtcttttgcctcttttcgatcctccaaggcatgattctgctgaaactatcagaagggctctcaatcttggagttaatgtgaagatgatcacaggagatcaacttgctatcggtaaagaaaccggaagaaggttgggaatgggtactaatatgtatccttcttcagctctcttaggtactcacaaggatgctaacctcgcatctattccagttgaagagttaatcgagaaagctgatggttttgcaggagttttccctgagcataagtacgaaatcgtgaagaaacttcaggaaagaaagcatattgttggtatgacaggagatggtgttaatgatgcaccagctttgaagaaagctgatataggaattgcagttgctgatgcaactgatgctgcaagaggtgcttctgatattgtgcttacagagcctggattgtctgttataatttcagcagtgttgacaagtagagctatcttccaaaggatgaaaaactacactatctatgctgtgtctatcacaataagaattgttttcggtttcatgcttatcgctttgatatg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1537029_sequence/1
type: Sequence
persistent identity: BBa K1537029 sequence
display id: BBa_K1537029_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atatgaagatgaagatgaaatatttggtgtgtcaaataaaaaccttgtgtggttaagtttgtgtttttttcttggcttgttgtgttatgaatttgtggctttttctaatattaaatgaatgtaagatcacattataatgaataaacaaatgtttctataatccattgtgaatgttttgttggatctcttctgcaccatataactactgtatgtgctatggtatggactatggaatatgattaaagataag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1537030_sequence/1
type: Sequence
persistent identity: BBa K1537030 sequence
display id: BBa_K1537030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcgacaagctctattttctccataataatgtgtgagtagttcccagataagggaattagggttcctatagggtttcgctcatgtgttgagcatataagaaacccttagtatgtatttgtatttgtaaaatacttctatcaataaaatttctaattcctaaaaccaaaatccagtactaaaatccagatcccccgaattaaatcggcgttaattcag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1537031_sequence/1
type: Sequence
persistent identity: BBa K1537031 sequence
display id: BBa_K1537031_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgttcaaacatttggcaataaagtttcttaagattgaatcctgttgccggtcttgcgatgattatcatataatttctgttgaattacgttaagcatgtaataattaacatgtaatgcatgacgttatttatgagatgggtttttatgattagagtcccgcaattatacatttaatacgcgatagaaaacaaaatatagcgcgcaaactaggataaattatcgcgcgcggtgtcatctatgttactagatcggg
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z