BBa_K1539001 1 BBa_K1539001 HPromoter->HRBS->mCherry 2014-10-08T11:00:00Z 2015-05-08T01:10:52Z mCherry from BBa_J0650 Promoter and RBS originated from primers Coding sequence has a high efficiency promoter followed by a high efficiency RBS to express fluorescent protein mCherry. false false _1921_ 0 23019 9 It's complicated false RBS primer construction had to maintain EcoRI and XbaI site. Promoter primer construction had to eliminate the EcoRI site but once ligated to the backbone, this site was reestablished. false Coleen Tran annotation2430094 1 BBa_K1539021 range2430094 1 44 56 annotation2430093 1 BBa_K1539055 range2430093 1 1 35 annotation2430095 1 BBa_J06504 range2430095 1 63 773 BBa_K1539001_sequence 1 ttgacagctagctcagtcctaggtactgtgctagctactagagaaagagaggaatgtactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z