BBa_K1539002 1 BBa_K1539002 LPromoter->LRBS->mCherry 2014-10-08T11:00:00Z 2015-05-08T01:10:52Z mCherry from BBa_J06504 RBS and promoter from created primers This coding sequence contains a low efficiency promoter followed by a low efficiency RBS allowing for the expression of mCherry false false _1921_ 0 23019 9 It's complicated false RBS primer construction needed to maintain the EcoRI and XbaI site. The promoter primer design could not maintain the EcoRI site but does contain the XbaI sequence. EcoRI was reestablished when the entire coding sequence was ligated to the backbone. false Coleen Tran annotation2430101 1 BBa_K1539034 range2430101 1 44 56 annotation2430100 1 BBa_K1539089 range2430100 1 1 35 annotation2430102 1 BBa_J06504 range2430102 1 63 773 BBa_K1539002_sequence 1 ttgacagctagctcagtcctagggattgtgctagctactagagaaagagtggacgctactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z