BBa_K1539002
1
BBa_K1539002
LPromoter->LRBS->mCherry
2014-10-08T11:00:00Z
2015-05-08T01:10:52Z
mCherry from BBa_J06504
RBS and promoter from created primers
This coding sequence contains a low efficiency promoter followed by a low efficiency RBS allowing for the expression of mCherry
false
false
_1921_
0
23019
9
It's complicated
false
RBS primer construction needed to maintain the EcoRI and XbaI site. The promoter primer design could not maintain the EcoRI site but does contain the XbaI sequence. EcoRI was reestablished when the entire coding sequence was ligated to the backbone.
false
Coleen Tran
annotation2430101
1
BBa_K1539034
range2430101
1
44
56
annotation2430100
1
BBa_K1539089
range2430100
1
1
35
annotation2430102
1
BBa_J06504
range2430102
1
63
773
BBa_K1539002_sequence
1
ttgacagctagctcagtcctagggattgtgctagctactagagaaagagtggacgctactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z