BBa_K1548000 1 BBa_K1548000 Hsp60 promoter (M. bovis) 2014-10-06T11:00:00Z 2015-05-08T01:10:53Z Genome Constitutive promoter from m. Enternamehere expected to function in p. Acnes. false false _1932_ 0 21656 9 It's complicated false None false Michael Jacus BBa_K1491008 1 BBa_K1491008 mRFP-His 2014-07-14T11:00:00Z 2015-05-08T01:10:44Z mRFP is BBa_E1010 mRFP (BBa_E1010) with 6x His tag for protein purification false false _1871_ 0 22521 9 Not in stock false RFP+His false Danielle Peters annotation2380109 1 6x His range2380109 1 676 693 annotation2380108 1 BBa_E1010 range2380108 1 1 675 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1548002 1 BBa_K1548002 Hsp60 Promoter-RBS-mRFP1-6xHisTag 2014-10-06T11:00:00Z 2015-05-08T01:10:53Z iGEM Registry Drives constitutive expression of mRFP1-6xHisTag protein using the Hsp60 promoter from M. bovis, intended for use in P. acnes (Combination of K1548000 and K1491009). false false _1932_ 0 3382 9 It's complicated false None false Jason Lohmueller component2399959 1 BBa_K1491009 component2399953 1 BBa_K1548000 annotation2399959 1 BBa_K1491009 range2399959 1 369 1085 annotation2399953 1 BBa_K1548000 range2399953 1 1 360 BBa_K1491009 1 BBa_K1491009 RBS-mRFP-His 2014-07-14T11:00:00Z 2015-05-08T01:10:44Z RBS is BBa_B0034 and mRFP is BBa_E1010 mRFP with 6x His tag (BBa_K1491008) and a strong RBS (BBa_B0034). It can be used as a reporter. false false _1871_ 0 22521 9 In stock false RBS+RFP+His false Danielle Peters component2380114 1 BBa_K1491008 component2380111 1 BBa_B0034 annotation2380111 1 BBa_B0034 range2380111 1 1 12 annotation2380114 1 BBa_K1491008 range2380114 1 19 717 BBa_K1548002_sequence 1 acggtgaccacaacgcgcccgctttgatcggggacgtctgcggccgaccatttacgggtcttgttgtcgttggcggtcatgggccgaacatactcacccggatcggagggccgaggacaaggtcgaacgaggggcatgacccggtgcggggcttcttgcactcggcataggcgagtgctaagaataacgttggcactcgcgaccggtgagtgctaggtcgggacggtgaggccaggcccgtcgtcgcagcgagtggcagcgaggacaacttgagccgtccgtcgcgggcactgcgcccggccagcgtaagtagcggggttgccgtcacccggtgacccccgtttcatccccgatgccggatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgctcatcatcaccaccatcactaataa BBa_B0034_sequence 1 aaagaggagaaa BBa_K1548000_sequence 1 acggtgaccacaacgcgcccgctttgatcggggacgtctgcggccgaccatttacgggtcttgttgtcgttggcggtcatgggccgaacatactcacccggatcggagggccgaggacaaggtcgaacgaggggcatgacccggtgcggggcttcttgcactcggcataggcgagtgctaagaataacgttggcactcgcgaccggtgagtgctaggtcgggacggtgaggccaggcccgtcgtcgcagcgagtggcagcgaggacaacttgagccgtccgtcgcgggcactgcgcccggccagcgtaagtagcggggttgccgtcacccggtgacccccgtttcatccccgatgccgga BBa_K1491008_sequence 1 atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgctcatcatcaccaccatcactaataa BBa_K1491009_sequence 1 aaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgctcatcatcaccaccatcactaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z