LOCUS       BBa_K1548003            3147 bp    DNA     linear       22-Dec-2024
DEFINITION  FixK2 Promoter-RBS-mRFP1-Hsp60 Promoter-RBS-YF1-RBS-FixJ
ACCESSION   BBa_K1548003
VERSION     BBa_K1548003.1  
FEATURES             Location/Qualifiers
     promoter        1..250
                     /label=FixK2 promoter
     promoter        1..250
                     /label=BBa_K592006
     misc_feature    263..266
                     /label=conserved
     RBS             259..270
                     /label=BBa_B0034
     misc_feature    277..951
                     /label=BBa_E1010
     misc_feature    952..969
                     /label=6x His
     CDS             277..975
                     /label=BBa_K1491008
     misc_feature    259..975
                     /label=BBa_K1491009
     promoter        984..1343
                     /label=BBa_K1548000
     misc_feature    1356..1359
                     /label=conserved
     RBS             1352..1363
                     /label=BBa_B0034
     CDS             1370..2500
                     /label=YF1
     CDS             1370..2500
                     /label=BBa_K592004
     misc_feature    2513..2516
                     /label=conserved
     RBS             2509..2520
                     /label=BBa_B0034
     CDS             2527..3147
                     /label=FixJ
     CDS             2527..3147
                     /label=BBa_K592005
     misc_feature    1352..3147
                     /label=BBa_K592016
ORIGIN
        1 gccggagccg attatccgca cccgtccttg gtcttggaca cactcgctcg cgatccgaag
       61 ccgcctttca aagatactcc gcagtgaaac gcatctttta agcgcgactt gtcgccttcg
      121 cgcagcagaa cactcgtatg gcactcaatc catgatcgct tcgttcgctc cgacgcgcgt
      181 tgcggggccc ccctcgaccg gatgacaaca cattgcgtca cctcaactgc tgcgcgcgca
      241 ctgcgtcaca tactagagaa agaggagaaa tactagatgg cttcctccga agacgttatc
      301 aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca cgagttcgaa
      361 atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt
      421 accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt
      481 tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact gtccttcccg
      541 gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt taccgttacc
      601 caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg tggtaccaac
      661 ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa
      721 cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa
      781 gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa accggttcag
      841 ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa cgaagactac
      901 accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc tcatcatcac
      961 caccatcact aataatacta gagacggtga ccacaacgcg cccgctttga tcggggacgt
     1021 ctgcggccga ccatttacgg gtcttgttgt cgttggcggt catgggccga acatactcac
     1081 ccggatcgga gggccgagga caaggtcgaa cgaggggcat gacccggtgc ggggcttctt
     1141 gcactcggca taggcgagtg ctaagaataa cgttggcact cgcgaccggt gagtgctagg
     1201 tcgggacggt gaggccaggc ccgtcgtcgc agcgagtggc agcgaggaca acttgagccg
     1261 tccgtcgcgg gcactgcgcc cggccagcgt aagtagcggg gttgccgtca cccggtgacc
     1321 cccgtttcat ccccgatgcc ggatactaga gaaagaggag aaatactaga tggctagttt
     1381 tcaatcattt gggataccag gacagctgga agtcatcaaa aaagcacttg atcacgtgcg
     1441 agtcggtgtg gtaattacag atcccgcact tgaagataat cctattgtct acgtaaatca
     1501 aggctttgtt caaatgaccg gctacgagac cgaggaaatt ttaggaaaga actgtcgctt
     1561 cttacagggg aaacacacag atccggcaga agtggacaac atcagaaccg ctttacaaaa
     1621 taaagaaccg gtcaccgttc agatccaaaa ctacaaaaaa gacggaacga tgttctggaa
     1681 tgaattaaat attgatccaa tggaaataga ggataaaacg tattttgtcg gcattcagaa
     1741 tgatatcacc gagcaccagc agacccaggc gcgtctccag gaactgcaat ccgagctcgt
     1801 ccacgtctcc aggctgagcg ccatgggcga aatggcgtcc gcgctcgcgc acgagctcaa
     1861 ccagccgctg gcggcgatca gcaactacat gaagggctcg cggcggctgc ttgccggcag
     1921 cagtgatccg aacacaccga aggtcgaaag cgccctggac cgcgccgccg agcaggcgct
     1981 gcgcgccggc cagatcatcc ggcgcctgcg cgacttcgtt gcccgcggcg aatcggagaa
     2041 gcgggtcgag agtctctcca agctgatcga ggaggccggc gcgctcgggc ttgccggcgc
     2101 gcgcgagcag aacgtgcagc tccgcttcag tctcgatccg ggcgccgatc tcgttctcgc
     2161 cgaccgggtg cagatccagc aggtcctggt caacctgttc cgcaacgcgc tggaagcgat
     2221 ggctcagtcg cagcgacgcg agctcgtcgt caccaacacc cccgccgccg acgacatgat
     2281 cgaggtcgaa gtgtccgaca ccggcagcgg tttccaggac gacgtcattc cgaacctgtt
     2341 tcagactttc ttcaccacca aggacaccgg catgggcgtg ggactgtcca tcagccgctc
     2401 gatcatcgaa gctcacggcg ggcgcatgtg ggccgagagc aacgcatcgg gcggggcgac
     2461 cttccgcttc accctcccgg cagccgacga gaattaataa tactagagaa agaggagaaa
     2521 tactagatga cgaccaaggg acatatctac gtcatcgacg acgacgcggc gatgcgggat
     2581 tcgctgaatt tcctgctgga ttctgccggc ttcggcgtca cgctgtttga cgacgcgcaa
     2641 gcctttctcg acgccctgcc gggtctctcc ttcggctgcg tcgtctccga cgtgcgcatg
     2701 ccgggccttg acggcatcga gctgttgaag cggatgaagg cgcagcaaag cccctttccg
     2761 atcctcatca tgaccggtca cggcgacgtg ccgctcgcgg tcgaggcgat gaagttaggg
     2821 gcggtcgact ttctggaaaa gcctttcgag gacgaccgcc tcaccgccat gatcgaatcg
     2881 gcgatccgcc aggccgagcc ggccgccaag agcgaggccg tcgcgcagga tatcgccgcc
     2941 cgcgtcgcct cgttgagccc cagggagcgc caggtcatgg aagggctgat cgccggcctt
     3001 tccaacaagc tgatcgcccg cgagtacgac atcagcccgc gcaccatcga ggtgtatcgg
     3061 gccaacgtca tgaccaagat gcaggccaac agcctttcgg agctggttcg cctcgcgatg
     3121 cgcgccggca tgctcaacga ttaataa
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