BBa_K1548003
type: ComponentDefinition
persistent identity: BBa K1548003
display id: BBa_K1548003
version: 1
was generated by: iGEM to SBOL conversion
description: FixK2 Promoter-RBS-mRFP1-Hsp60 Promoter-RBS-YF1-RBS-FixJ
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:53Z
mutable provenance: iGEM Regsitry
owned by: james
owned by: myers
mutable description: System for blue light-induced expression of mRFP1 in P. acnes using constitutive Hsp60 promoter from M. bovis (expected to work in P. acnes) to drive expression of blue light-regulated transcription factors YF1 and FixJ.
discontinued: false
dominant: false
group u list: _1932_
m user id: 0
owner id: 21656
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: zero.
star: false
creator: Michael Jacus
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_K1548000
component: BBa_K1491009
component: BBa_K592006
component: BBa_K592016
sequence annotation: BBa_K592006
sequence annotation: BBa_K1491009
sequence annotation: BBa_K1548000
sequence annotation: BBa_K592016
sequence: https://synbiohub.org/public/igem/BBa_K1548003_sequence/1

BBa_K1548000
type: Component
persistent identity: component2399968
display id: component2399968
version: 1
top level: BBa_K1548003
definition: BBa_K1548000
access: public

BBa_K1491009
type: Component
persistent identity: component2399967
display id: component2399967
version: 1
top level: BBa_K1548003
definition: BBa_K1491009
access: public

BBa_K592006
type: Component
persistent identity: component2399961
display id: component2399961
version: 1
top level: BBa_K1548003
definition: BBa_K592006
access: public

BBa_K592016
type: Component
persistent identity: component2399977
display id: component2399977
version: 1
top level: BBa_K1548003
definition: BBa_K592016
access: public

BBa_K592006
type: SequenceAnnotation
persistent identity: annotation2399961
display id: annotation2399961
version: 1
top level: BBa_K1548003
location: https://synbiohub.org/public/igem/BBa_K1548003/annotation2399961/range2399961/1
component: BBa_K592006

https://synbiohub.org/public/igem/BBa_K1548003/annotation2399961/range2399961/1
type: Range
persistent identity: range2399961
display id: range2399961
version: 1
top level: BBa_K1548003
direction: direction/reverse
start: 1
end: 250
orientation: inline

BBa_K1491009
type: SequenceAnnotation
persistent identity: annotation2399967
display id: annotation2399967
version: 1
top level: BBa_K1548003
location: https://synbiohub.org/public/igem/BBa_K1548003/annotation2399967/range2399967/1
component: BBa_K1491009

https://synbiohub.org/public/igem/BBa_K1548003/annotation2399967/range2399967/1
type: Range
persistent identity: range2399967
display id: range2399967
version: 1
top level: BBa_K1548003
direction: direction/reverse
start: 259
end: 975
orientation: inline

BBa_K1548000
type: SequenceAnnotation
persistent identity: annotation2399968
display id: annotation2399968
version: 1
top level: BBa_K1548003
location: https://synbiohub.org/public/igem/BBa_K1548003/annotation2399968/range2399968/1
component: BBa_K1548000

https://synbiohub.org/public/igem/BBa_K1548003/annotation2399968/range2399968/1
type: Range
persistent identity: range2399968
display id: range2399968
version: 1
top level: BBa_K1548003
direction: direction/reverse
start: 984
end: 1343
orientation: inline

BBa_K592016
type: SequenceAnnotation
persistent identity: annotation2399977
display id: annotation2399977
version: 1
top level: BBa_K1548003
location: https://synbiohub.org/public/igem/BBa_K1548003/annotation2399977/range2399977/1
component: BBa_K592016

https://synbiohub.org/public/igem/BBa_K1548003/annotation2399977/range2399977/1
type: Range
persistent identity: range2399977
display id: range2399977
version: 1
top level: BBa_K1548003
direction: direction/reverse
start: 1352
end: 3147
orientation: inline

BBa_K1491009
type: ComponentDefinition
persistent identity: BBa K1491009
display id: BBa_K1491009
version: 1
was generated by: iGEM to SBOL conversion
description: RBS-mRFP-His
created: 2014-07-14T11:00:00Z
modified: 2015-05-08T01:10:44Z
mutable provenance: RBS is BBa_B0034 and mRFP is BBa_E1010
owned by: james
owned by: myers
mutable description: mRFP with 6x His tag (BBa_K1491008) and a strong RBS (BBa_B0034). It can be used as a reporter.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1871_
m user id: 0
owner id: 22521
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: RBS+RFP+His
star: false
creator: Danielle Peters
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1491008
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K1491008
sequence: https://synbiohub.org/public/igem/BBa_K1491009_sequence/1

BBa_K1491008
type: Component
persistent identity: component2380114
display id: component2380114
version: 1
top level: BBa_K1491009
definition: BBa_K1491008
access: public

BBa_B0034
type: Component
persistent identity: component2380111
display id: component2380111
version: 1
top level: BBa_K1491009
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2380111
display id: annotation2380111
version: 1
top level: BBa_K1491009
location: https://synbiohub.org/public/igem/BBa_K1491009/annotation2380111/range2380111/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1491009/annotation2380111/range2380111/1
type: Range
persistent identity: range2380111
display id: range2380111
version: 1
top level: BBa_K1491009
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_K1491008
type: SequenceAnnotation
persistent identity: annotation2380114
display id: annotation2380114
version: 1
top level: BBa_K1491009
location: https://synbiohub.org/public/igem/BBa_K1491009/annotation2380114/range2380114/1
component: BBa_K1491008

https://synbiohub.org/public/igem/BBa_K1491009/annotation2380114/range2380114/1
type: Range
persistent identity: range2380114
display id: range2380114
version: 1
top level: BBa_K1491009
direction: direction/reverse
start: 19
end: 717
orientation: inline

BBa_K1548000
type: ComponentDefinition
persistent identity: BBa K1548000
display id: BBa_K1548000
version: 1
was generated by: iGEM to SBOL conversion
description: Hsp60 promoter (M. bovis)
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:53Z
mutable provenance: Genome
owned by: james
owned by: myers
mutable description: Constitutive promoter from m. Enternamehere expected to function in p. Acnes.
discontinued: false
dominant: false
experience: experience/None
group u list: _1932_
m user id: 0
owner id: 21656
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: None
star: false
creator: Michael Jacus
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_K1548000_sequence/1

BBa_K592006
type: ComponentDefinition
persistent identity: BBa K592006
display id: BBa_K592006
version: 1
was generated by: iGEM to SBOL conversion
description: FixK2 promoter
created: 2011-09-16T11:00:00Z
modified: 2015-05-08T01:12:48Z
mutable provenance: FixK2 promoter omes from the genome of Bradyrhizobium japonicum.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: FixK2 promoter is the wild-type promoter to which phospho-FixJ binds. FixJ in turn can be regulated by YF1, the blue light-sensing protein. This promoter shows very little leaky activity in the absence of FixJ.
discontinued: false
dominant: false
group u list: _763_
m user id: 0
owner id: 7929
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The sequence information was provided by Moffat (Moffat et al. 2009). The DNA was synthesized by GenScript.
star: false
creator: Lei Sun
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: FixK2 promoter
sequence: https://synbiohub.org/public/igem/BBa_K592006_sequence/1

FixK2 promoter
type: SequenceAnnotation
persistent identity: annotation2130179
display id: annotation2130179
version: 1
top level: BBa_K592006
location: https://synbiohub.org/public/igem/BBa_K592006/annotation2130179/range2130179/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K592006/annotation2130179/range2130179/1
type: Range
persistent identity: range2130179
display id: range2130179
version: 1
top level: BBa_K592006
direction: direction/forward
start: 1
end: 250
orientation: inline

BBa_K592016
type: ComponentDefinition
persistent identity: BBa K592016
display id: BBa_K592016
version: 1
was generated by: iGEM to SBOL conversion
description: B0034-YF1-B0034-FixJ, blue light sensor and RR with RBS
created: 2011-09-20T11:00:00Z
modified: 2015-05-08T01:12:49Z
mutable provenance: The components that made up of YF1 fusion protein comes from Bradyrhizobium japonicum and Bacillus subtilis. FixJ comes from the genome of Bradyrhizobium japonicum.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: YF1 works in conjunction with its response regulator, FixJ. This part consists of RBS-coupled YF1 and FixJ without promoter.
discontinued: false
dominant: false
experience: experience/Works
group u list: _763_
m user id: 0
owner id: 7929
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This constructs Strong RBS to ensure good expression.
star: false
creator: Lei Sun
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K592004
component: BBa_B0034
component: BBa_K592005
sequence annotation: BBa_B0034
sequence annotation: BBa_K592004
sequence annotation: BBa_K592005
sequence: https://synbiohub.org/public/igem/BBa_K592016_sequence/1

BBa_K592004
type: Component
persistent identity: component2136335
display id: component2136335
version: 1
top level: BBa_K592016
definition: YF1
access: public

BBa_B0034
type: Component
persistent identity: component2136333
display id: component2136333
version: 1
top level: BBa_K592016
definition: BBa_B0034
access: public

BBa_K592005
type: Component
persistent identity: component2136339
display id: component2136339
version: 1
top level: BBa_K592016
definition: FixJ
access: public

BBa_B0034
type: Component
persistent identity: component2136337
display id: component2136337
version: 1
top level: BBa_K592016
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2136333
display id: annotation2136333
version: 1
top level: BBa_K592016
location: https://synbiohub.org/public/igem/BBa_K592016/annotation2136333/range2136333/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K592016/annotation2136333/range2136333/1
type: Range
persistent identity: range2136333
display id: range2136333
version: 1
top level: BBa_K592016
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_K592004
type: SequenceAnnotation
persistent identity: annotation2136335
display id: annotation2136335
version: 1
top level: BBa_K592016
location: https://synbiohub.org/public/igem/BBa_K592016/annotation2136335/range2136335/1
component: BBa_K592004

https://synbiohub.org/public/igem/BBa_K592016/annotation2136335/range2136335/1
type: Range
persistent identity: range2136335
display id: range2136335
version: 1
top level: BBa_K592016
direction: direction/reverse
start: 19
end: 1149
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2136337
display id: annotation2136337
version: 1
top level: BBa_K592016
location: https://synbiohub.org/public/igem/BBa_K592016/annotation2136337/range2136337/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K592016/annotation2136337/range2136337/1
type: Range
persistent identity: range2136337
display id: range2136337
version: 1
top level: BBa_K592016
direction: direction/reverse
start: 1158
end: 1169
orientation: inline

BBa_K592005
type: SequenceAnnotation
persistent identity: annotation2136339
display id: annotation2136339
version: 1
top level: BBa_K592016
location: https://synbiohub.org/public/igem/BBa_K592016/annotation2136339/range2136339/1
component: BBa_K592005

https://synbiohub.org/public/igem/BBa_K592016/annotation2136339/range2136339/1
type: Range
persistent identity: range2136339
display id: range2136339
version: 1
top level: BBa_K592016
direction: direction/reverse
start: 1176
end: 1796
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_K1491008
type: ComponentDefinition
persistent identity: BBa K1491008
display id: BBa_K1491008
version: 1
was generated by: iGEM to SBOL conversion
description: mRFP-His
created: 2014-07-14T11:00:00Z
modified: 2015-05-08T01:10:44Z
mutable provenance: mRFP is BBa_E1010
owned by: james
owned by: myers
mutable description: mRFP (BBa_E1010) with 6x His tag for protein purification
discontinued: false
dominant: false
experience: experience/Works
group u list: _1871_
m user id: 0
owner id: 22521
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: RFP+His
star: false
creator: Danielle Peters
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: BBa_E1010
sequence annotation: 6x His
sequence: https://synbiohub.org/public/igem/BBa_K1491008_sequence/1

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2380108
display id: annotation2380108
version: 1
top level: BBa_K1491008
location: https://synbiohub.org/public/igem/BBa_K1491008/annotation2380108/range2380108/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K1491008/annotation2380108/range2380108/1
type: Range
persistent identity: range2380108
display id: range2380108
version: 1
top level: BBa_K1491008
direction: direction/forward
start: 1
end: 675
orientation: inline

6x His
type: SequenceAnnotation
persistent identity: annotation2380109
display id: annotation2380109
version: 1
top level: BBa_K1491008
location: https://synbiohub.org/public/igem/BBa_K1491008/annotation2380109/range2380109/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_K1491008/annotation2380109/range2380109/1
type: Range
persistent identity: range2380109
display id: range2380109
version: 1
top level: BBa_K1491008
direction: direction/forward
start: 676
end: 693
orientation: inline

YF1
type: ComponentDefinition
persistent identity: BBa K592004
display id: BBa_K592004
version: 1
was generated by: iGEM to SBOL conversion
description: YF1 blue-light sensor protein
created: 2011-09-16T11:00:00Z
modified: 2015-05-08T01:12:48Z
mutable provenance: Ytva comes from the genome of Bacillus subtilis. FixL derives from the genome of Bradyrhizobium japonicum.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: YF1 is a fusion protein of YtvA (B.subtilis) and FixL(Br.japonicum). It contains the N-terminal region of YtvA (1-127) and C-terminal region of FixL (258-505), interconnected by a helical linker. This fusion protein is designed by Andreas Moglich and Keith Moffat. As shown in previous research, Illumination of the fusion kinase YF1 reduced net kinase activity by ∼1000-fold in vitro. YF1 also controls gene expression in a light-dependent manner in vivo.
discontinued: false
dominant: false
experience: experience/None
group u list: _763_
m user id: 0
owner id: 7929
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The sequence information was provided in Moglich and Moffat's article. The physical DNA was synthesized by GenScript. Codon-optimization may enhance its expression.
star: false
creator: Lei Sun
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: YF1
sequence: https://synbiohub.org/public/igem/BBa_K592004_sequence/1

YF1
type: SequenceAnnotation
persistent identity: annotation2130111
display id: annotation2130111
version: 1
top level: YF1
location: https://synbiohub.org/public/igem/BBa_K592004/annotation2130111/range2130111/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K592004/annotation2130111/range2130111/1
type: Range
persistent identity: range2130111
display id: range2130111
version: 1
top level: YF1
direction: direction/forward
start: 1
end: 1131
orientation: inline

FixJ
type: ComponentDefinition
persistent identity: BBa K592005
display id: BBa_K592005
version: 1
was generated by: iGEM to SBOL conversion
description: FixJ, the response regulator to YF1
created: 2011-09-16T11:00:00Z
modified: 2015-05-08T01:12:48Z
mutable provenance: FixJ comes from the genome of Bradyrhizobium japonicum.
owned by: james
owned by: myers
mutable description: FixJ comes from the genome of Bradyrhizobium japonicum. It's the wild-type response regulator to YF1, the blue light-sensing fusion protein.
discontinued: false
dominant: false
experience: experience/Works
group u list: _763_
m user id: 0
owner id: 7929
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: The sequence was information was provided by Moffat (Moffat et al. 2009). The DNA was synthesized by GenScript.
star: false
creator: Lei Sun
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: FixJ
sequence: https://synbiohub.org/public/igem/BBa_K592005_sequence/1

FixJ
type: SequenceAnnotation
persistent identity: annotation2130114
display id: annotation2130114
version: 1
top level: FixJ
location: https://synbiohub.org/public/igem/BBa_K592005/annotation2130114/range2130114/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K592005/annotation2130114/range2130114/1
type: Range
persistent identity: range2130114
display id: range2130114
version: 1
top level: FixJ
direction: direction/forward
start: 1
end: 621
orientation: inline

https://synbiohub.org/public/igem/BBa_K1548003_sequence/1
type: Sequence
persistent identity: BBa K1548003 sequence
display id: BBa_K1548003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gccggagccgattatccgcacccgtccttggtcttggacacactcgctcgcgatccgaagccgcctttcaaagatactccgcagtgaaacgcatcttttaagcgcgacttgtcgccttcgcgcagcagaacactcgtatggcactcaatccatgatcgcttcgttcgctccgacgcgcgttgcggggcccccctcgaccggatgacaacacattgcgtcacctcaactgctgcgcgcgcactgcgtcacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgctcatcatcaccaccatcactaataatactagagacggtgaccacaacgcgcccgctttgatcggggacgtctgcggccgaccatttacgggtcttgttgtcgttggcggtcatgggccgaacatactcacccggatcggagggccgaggacaaggtcgaacgaggggcatgacccggtgcggggcttcttgcactcggcataggcgagtgctaagaataacgttggcactcgcgaccggtgagtgctaggtcgggacggtgaggccaggcccgtcgtcgcagcgagtggcagcgaggacaacttgagccgtccgtcgcgggcactgcgcccggccagcgtaagtagcggggttgccgtcacccggtgacccccgtttcatccccgatgccggatactagagaaagaggagaaatactagatggctagttttcaatcatttgggataccaggacagctggaagtcatcaaaaaagcacttgatcacgtgcgagtcggtgtggtaattacagatcccgcacttgaagataatcctattgtctacgtaaatcaaggctttgttcaaatgaccggctacgagaccgaggaaattttaggaaagaactgtcgcttcttacaggggaaacacacagatccggcagaagtggacaacatcagaaccgctttacaaaataaagaaccggtcaccgttcagatccaaaactacaaaaaagacggaacgatgttctggaatgaattaaatattgatccaatggaaatagaggataaaacgtattttgtcggcattcagaatgatatcaccgagcaccagcagacccaggcgcgtctccaggaactgcaatccgagctcgtccacgtctccaggctgagcgccatgggcgaaatggcgtccgcgctcgcgcacgagctcaaccagccgctggcggcgatcagcaactacatgaagggctcgcggcggctgcttgccggcagcagtgatccgaacacaccgaaggtcgaaagcgccctggaccgcgccgccgagcaggcgctgcgcgccggccagatcatcc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1491009_sequence/1
type: Sequence
persistent identity: BBa K1491009 sequence
display id: BBa_K1491009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgctcatcatcaccaccatcactaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1548000_sequence/1
type: Sequence
persistent identity: BBa K1548000 sequence
display id: BBa_K1548000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acggtgaccacaacgcgcccgctttgatcggggacgtctgcggccgaccatttacgggtcttgttgtcgttggcggtcatgggccgaacatactcacccggatcggagggccgaggacaaggtcgaacgaggggcatgacccggtgcggggcttcttgcactcggcataggcgagtgctaagaataacgttggcactcgcgaccggtgagtgctaggtcgggacggtgaggccaggcccgtcgtcgcagcgagtggcagcgaggacaacttgagccgtccgtcgcgggcactgcgcccggccagcgtaagtagcggggttgccgtcacccggtgacccccgtttcatccccgatgccgga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K592006_sequence/1
type: Sequence
persistent identity: BBa K592006 sequence
display id: BBa_K592006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gccggagccgattatccgcacccgtccttggtcttggacacactcgctcgcgatccgaagccgcctttcaaagatactccgcagtgaaacgcatcttttaagcgcgacttgtcgccttcgcgcagcagaacactcgtatggcactcaatccatgatcgcttcgttcgctccgacgcgcgttgcggggcccccctcgaccggatgacaacacattgcgtcacctcaactgctgcgcgcgcactgcgtcaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K592016_sequence/1
type: Sequence
persistent identity: BBa K592016 sequence
display id: BBa_K592016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggctagttttcaatcatttgggataccaggacagctggaagtcatcaaaaaagcacttgatcacgtgcgagtcggtgtggtaattacagatcccgcacttgaagataatcctattgtctacgtaaatcaaggctttgttcaaatgaccggctacgagaccgaggaaattttaggaaagaactgtcgcttcttacaggggaaacacacagatccggcagaagtggacaacatcagaaccgctttacaaaataaagaaccggtcaccgttcagatccaaaactacaaaaaagacggaacgatgttctggaatgaattaaatattgatccaatggaaatagaggataaaacgtattttgtcggcattcagaatgatatcaccgagcaccagcagacccaggcgcgtctccaggaactgcaatccgagctcgtccacgtctccaggctgagcgccatgggcgaaatggcgtccgcgctcgcgcacgagctcaaccagccgctggcggcgatcagcaactacatgaagggctcgcggcggctgcttgccggcagcagtgatccgaacacaccgaaggtcgaaagcgccctggaccgcgccgccgagcaggcgctgcgcgccggccagatcatccggcgcctgcgcgacttcgttgcccgcggcgaatcggagaagcgggtcgagagtctctccaagctgatcgaggaggccggcgcgctcgggcttgccggcgcgcgcgagcagaacgtgcagctccgcttcagtctcgatccgggcgccgatctcgttctcgccgaccgggtgcagatccagcaggtcctggtcaacctgttccgcaacgcgctggaagcgatggctcagtcgcagcgacgcgagctcgtcgtcaccaacacccccgccgccgacgacatgatcgaggtcgaagtgtccgacaccggcagcggtttccaggacgacgtcattccgaacctgtttcagactttcttcaccaccaaggacaccggcatgggcgtgggactgtccatcagccgctcgatcatcgaagctcacggcgggcgcatgtgggccgagagcaacgcatcgggcggggcgaccttccgcttcaccctcccggcagccgacgagaattaataatactagagaaagaggagaaatactagatgacgaccaagggacatatctacgtcatcgacgacgacgcggcgatgcgggattcgctgaatttcctgctggattctgccggcttcggcgtcacgctgtttgacgacgcgcaagcctttctcgacgccctgccgggtctctccttcggctgcgtcgtctccgacgtgcgcatgccgggccttgacggcatcgagctgttgaagcggatgaaggcgcagcaaagcccctttccgatcctcatcatgaccggtcacggcgacgtgccgctcgcggtcgaggcgatgaagttaggggcggtcgactttctggaaaagcctttcgaggacgaccgcctcaccgccatgatcgaatcggcgatccgccaggccgagccggccgccaagagcgaggccgtcgcgcaggatatcgccgcccgcgtcgcctcgttgagccccagggagcgccaggtcatggaagggctgatcgccggcctttccaacaagctgatcgcccgcgagtacgacatcagcccgcgcaccatcgaggtgtatcgggccaacgtcatgaccaagatgcaggccaacagcctttcggagctggttcgcctcgcgatgcgcgccggcatgctcaacgattaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1491008_sequence/1
type: Sequence
persistent identity: BBa K1491008 sequence
display id: BBa_K1491008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgctcatcatcaccaccatcactaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K592004_sequence/1
type: Sequence
persistent identity: BBa K592004 sequence
display id: BBa_K592004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggctagttttcaatcatttgggataccaggacagctggaagtcatcaaaaaagcacttgatcacgtgcgagtcggtgtggtaattacagatcccgcacttgaagataatcctattgtctacgtaaatcaaggctttgttcaaatgaccggctacgagaccgaggaaattttaggaaagaactgtcgcttcttacaggggaaacacacagatccggcagaagtggacaacatcagaaccgctttacaaaataaagaaccggtcaccgttcagatccaaaactacaaaaaagacggaacgatgttctggaatgaattaaatattgatccaatggaaatagaggataaaacgtattttgtcggcattcagaatgatatcaccgagcaccagcagacccaggcgcgtctccaggaactgcaatccgagctcgtccacgtctccaggctgagcgccatgggcgaaatggcgtccgcgctcgcgcacgagctcaaccagccgctggcggcgatcagcaactacatgaagggctcgcggcggctgcttgccggcagcagtgatccgaacacaccgaaggtcgaaagcgccctggaccgcgccgccgagcaggcgctgcgcgccggccagatcatccggcgcctgcgcgacttcgttgcccgcggcgaatcggagaagcgggtcgagagtctctccaagctgatcgaggaggccggcgcgctcgggcttgccggcgcgcgcgagcagaacgtgcagctccgcttcagtctcgatccgggcgccgatctcgttctcgccgaccgggtgcagatccagcaggtcctggtcaacctgttccgcaacgcgctggaagcgatggctcagtcgcagcgacgcgagctcgtcgtcaccaacacccccgccgccgacgacatgatcgaggtcgaagtgtccgacaccggcagcggtttccaggacgacgtcattccgaacctgtttcagactttcttcaccaccaaggacaccggcatgggcgtgggactgtccatcagccgctcgatcatcgaagctcacggcgggcgcatgtgggccgagagcaacgcatcgggcggggcgaccttccgcttcaccctcccggcagccgacgagaattaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K592005_sequence/1
type: Sequence
persistent identity: BBa K592005 sequence
display id: BBa_K592005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgacgaccaagggacatatctacgtcatcgacgacgacgcggcgatgcgggattcgctgaatttcctgctggattctgccggcttcggcgtcacgctgtttgacgacgcgcaagcctttctcgacgccctgccgggtctctccttcggctgcgtcgtctccgacgtgcgcatgccgggccttgacggcatcgagctgttgaagcggatgaaggcgcagcaaagcccctttccgatcctcatcatgaccggtcacggcgacgtgccgctcgcggtcgaggcgatgaagttaggggcggtcgactttctggaaaagcctttcgaggacgaccgcctcaccgccatgatcgaatcggcgatccgccaggccgagccggccgccaagagcgaggccgtcgcgcaggatatcgccgcccgcgtcgcctcgttgagccccagggagcgccaggtcatggaagggctgatcgccggcctttccaacaagctgatcgcccgcgagtacgacatcagcccgcgcaccatcgaggtgtatcgggccaacgtcatgaccaagatgcaggccaacagcctttcggagctggttcgcctcgcgatgcgcgccggcatgctcaacgattaataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z