LOCUS       BBa_K155000             2068 bp    DNA     linear       20-Apr-2024
DEFINITION  LIC plasmid
ACCESSION   BBa_K155000
VERSION     BBa_K155000.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=EcoRV site
     misc_feature    2066..2068
                     /label=EcoRV site
     misc_feature    1..15
                     /label=LIC overhang
     primer_bind     74..93
                     /label=VR
     CDS             177..792
                     /label=rep (pMB1) (rev)
     misc_feature    193..193
                     /label=ORI
     CDS             951..1812
                     /label=APr (rev)
     misc_feature    1847..1847
                     /label=pBLA (rev)
     primer_bind     1935..1954
                     /label=VF2
     misc_feature    2053..2068
                     /label=LIC overhang (rev)
     stem_loop       2024..2043
                     /label=terminator
ORIGIN
        1 atccctacta cctacggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
       61 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
      121 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
      181 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
      241 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
      301 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
      361 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
      421 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
      481 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
      541 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
      601 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
      661 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
      721 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
      781 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
      841 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
      901 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
      961 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     1021 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     1081 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     1141 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     1201 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     1261 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     1321 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     1381 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     1441 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     1501 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     1561 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     1621 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     1681 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     1741 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     1801 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     1861 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     1921 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     1981 taaaaatagg cgtatcacga ggcagaattt cagataaaaa aaatccttag ctttcgctaa
     2041 ggatgatttc tgcgatggta gtagggat
//