BBa_K1554005 1 BBa_K1554005 Blue biosafety module for plants 2014-09-28T11:00:00Z 2015-06-09T09:45:18Z TA29 promoter [http://parts.igem.org/Part:BBa_K1554000 Part:BBa_K1554000] Barnase [http://parts.igem.org/Part:BBa_J72173 Part:BBa_J72173] AmilGFP [Waiting for Norwich team part name in registry] P35s [http://parts.igem.org/Part:BBa_K1537015 Part:BBa_K1537015] This biosafety device for plants consists on two submodules: a male sterility submodule and an identity preservation submodule. The male sterility submodule consist on a barnase specifically expressed in anthers under the regulation of the TA29 tapetum-specific promoter. As result, pollen from these plants is not fertile. The identity preservation submodule expresses the blue chromoprotein AmilCP, so that plants can be visually differentiated from non-transgenic plants. false false _1939_ 4206 22386 9 It's complicated false The assembly was performed by GoldenBraid 2.0 assembly. The barnase was amplified by PCR from [http://parts.igem.org/Part:BBa_J72173 Part:BBa_J72173] and a mutation in T105P was introduced. false Valencia_UPV team members annotation2389556 1 T35S range2389556 1 3136 3361 annotation2389539 1 barnase range2389539 1 863 1195 annotation2389541 1 P35s range2389541 1 1438 2464 annotation2389538 1 TA29 promoter range2389538 1 4 858 annotation2389555 1 AmilCP range2389555 1 2469 3131 annotation2389540 1 T35s range2389540 1 1200 1425 BBa_K1554005_sequence 1 gagtagcgaatgcaattaatttagacattgtgttatgttccagttaaccgcttccctgcacttctttcaatctatctctcgatagaaaattgtgatactttgcgacttctatcagaggactttttgttttccatgtaacaatctgtcattttcgatggggagatttgcacaaataggctatttatgtgtcccaatttaaattttaaccccatgtcgatcagaacttagccacgagcaccagaagtttgatggatatgtgactttgtcactatccggtttactaatcaagagctatttttattcaaaattggatatctagctaagtataactggataatttgcattaacagattgaatatagtgccaaacaagaagggacaattgacttgtcactttatgaaagatgattcaaacatgattttttatgtactaatatatacatcctactcgaattaaagcgacataggctcgaagtatgcacatttagcaatgtaaattaaatcagtttttgaatcaagctaaaagcagacttgcataaggtgggtggctggactagaataaacatcttctctagcacagcttcataatgtaatttccataactgaaatcagggtgagacaaaattttggtactttttcctcacactaagtccatgtttgcaacaaattaatacatgaaaccttaatgttaccctcagattagcctgctactccccattttcctcgaaatgctccaacaaaagttagttttgcaagttgttgtgtatgtcttgtgctctatatatgcccttgtggtgcaagtgtaacagtacaacatcatcactcaaatcaaagtttttacttaaagaaattagctaaaaatggcacaggttatcaacacgtttgacggggttgcggattatcttcagacatatcataagctacctgataattacattacaaaatcagaagcacaagccctcggctgggtggcatcaaaagggaaccttgcagacgtcgctccggggaaaagcatcggcggagacatcttctcaaacagggaaggcaaactcccgggcaaaagcggacgaacatggcgtgaagcggatattaactatacatcaggcttcagaaattcagaccggattctttactcaagcgactggctgatttacaaaacaacggaccattatcagccctttacaaaaatcagataagcttcggccatgctagagtccgcaaaaatcaccagtctctctctacaaatctatctctctctatttttctccagaataatgtgtgagtagttcccagataagggaattagggttcttatagggtttcgctcatgtgttgagcatataagaaacccttagtatgtatttgtatttgtaaaatacttctatcaataaaatttctaattcctaaaaccaaaatccagtgacctcgctgtcaggagactagagccaagctgatctcctttgccccggagatcaccatggacgactttctctatctctacgatctaggaagaaagttcgacggagaaggtgacgataccatgttcaccaccgataatgagaagattagcctcttcaatttcagaaagaatgctgacccacagatggttagagaggcctacgcggcaggtctgatcaagacgatctacccgagtaataatctccaggagatcaaataccttcccaagaaggttaaagatgcagtcaaaagattcaggactaactgcatcaagaacacagagaaagatatatttctcaagatcagaagtactattccagtatggacgattcaaggcttgcttcataaaccaaggcaagtaatagagattggagtctctaagaaagtagttcctactgaatcaaaggccatggagtcaaaaattcagatcgaggatctaacagaactcgccgtgaagactggcgaacagttcatacagagtcttttacgactcaatgacaagaagaaaatcttcgtcaacatggtggagcacgacactctcgtctactccaagaatatcaaagatacagtctcagaagaccaaagggctattgagacttttcaacaaagggtaatatcgggaaacctcctcggattccattgcccagctatctgtcacttcatcaaaaggacagtagaaaaggaaggtggcacctacaaatgccatcattgcgataaaggaaaggctatcgttcaagatgcctctgccgacagtggtcccaaagatggacccccacccacgaggagcatcgtggaaaaagaagacgttccaaccacgtcttcaaagcaagtggattgatgtgatatctccactgacgtaagggatgacgcacaatcccactatccttcgcaagacccttcctctatataaggaagttcatttcatttggagaggactccggtatttttacaacaataccacaacaaaacaaacaacaaacaacattacaatttactattctagtcgaaatgagcgtgatcgctaagcagatgacctacaaggtgtacatgagcggaactgtgaacggtcactacttcgaggttgagggtgatggtaagggtaagccttatgagggtgagcagactgtgaagcttactgtgactaagggtggtcctcttcctttcgcttgggatattttgtctcctcagtgccagtacggtagcatccctttcactaagtaccctgaggatatccctgattacgtgaagcagtctttcccagagggttacacctgggagaggatcatgaatttcgaggatggtgctgtgtgcaccgtgagcaacgattcttctattcagggaaactgcttcatctaccacgtgaagttctccggtctgaacttccctcctaatggtcctgtgatgcagaagaaaactcagggttgggagcctaacaccgagaggctttttgctagggatggtatgctgctgggtaacaacttcatggctctgaagcttgagggtggtggacattacctttgcgagttcaagaccacttacaaggctaagaaacctgtgaagatgcctggttaccactatgtggatagaaagctggatgtgaccaaccacaacaaggattacaccagcgttgagcagtgcgagatctctattgctagaaagcctgtggtggcttaggcttcggccatgctagagtccgcaaaaatcaccagtctctctctacaaatctatctctctctatttttctccagaataatgtgtgagtagttcccagataagggaattagggttcttatagggtttcgctcatgtgttgagcatataagaaacccttagtatgtatttgtatttgtaaaatacttctatcaataaaatttctaattcctaaaaccaaaatccagtgacctcgc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z