BBa_K1555000 1 BBa_K1555000 The copa promoter wth a gfp reporter 2014-09-27T11:00:00Z 2015-05-08T01:10:53Z We attained this sensor from E.coli's genome. This part is our testing part.The copa is one of the candidates of our copper sensor.For comparing with other two sequences,marRAB and cueo,we linked a reporter(BBa_E0840) follow the promoter of copa.With the concentration of copper rises, the expression of downstream gfp coding gene increases.We designed two experiments to test those candidates' sensitivity and specificity individually by measure fluorescence intensity. false false _1940_ 0 22116 9 In stock false For guaranteeing our sensor is simple but copper-sensitive,we need to find a operator which directly or indirectly regulated by copper in copa sequence. false Xu Xuan annotation2387950 1 RBS-1\Strong range2387950 1 250 264 annotation2387946 1 copa promoter range2387946 1 1 241 annotation2387955 1 T7 TE range2387955 1 1049 1113 annotation2387956 1 polya range2387956 1 1113 1127 annotation2387947 1 BBa_E0840 range2387947 1 250 1127 annotation2387948 1 RBS range2387948 1 257 261 annotation2387957 1 stop range2387957 1 1120 1120 annotation2387953 1 stem_loop range2387953 1 1010 1053 annotation2387954 1 BBa_B0012 range2387954 1 1087 1127 annotation2387952 1 BBa_B0010 range2387952 1 999 1078 annotation2387951 1 BBa_B0030 range2387951 1 250 264 annotation2387949 1 BBa_E0040 range2387949 1 271 990 BBa_K1555000_sequence 1 cctttttatagatgcgggaggtaattcctcaccccggtgccgattttcaggcatcctgatttaacttagcacccgcaacttaactacaggaaaacaaagagataaatgtctaatcctgatgcaaatcgagccgattttttaatctttacggacttttacccgcctggtttattaatttcttgaccttccccttgctggaaggtttaacctttatcacagccagtcaaaactgtcttaaaggtactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z