LOCUS BBa_K1555008 4337 bp DNA linear 22-Nov-2024 DEFINITION sgRNA and dCAS9 ACCESSION BBa_K1555008 VERSION BBa_K1555008.1 FEATURES Location/Qualifiers misc_feature 1..54 /label=R0040 misc_feature 1..4180 /label=BBa_K1081000 misc_feature 1..74 /label=BBa_J13002 misc_feature 63..74 /label=B0034 CDS 77..4180 /label=dcas9 promoter 4189..4223 /label=BBa_J23100 misc_feature 4232..4251 /label=20nt target sequence stem_loop 4252..4337 /label=sgRNA scaffold ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaataatgg ataagaaata ctcaataggc ttagctatcg gcacaaatag 121 cgtcggatgg gcggtgatca ctgatgaata taaggttccg tctaaaaagt tcaaggttct 181 gggaaataca gaccgccaca gtatcaaaaa aaatcttata ggggctcttt tatttgacag 241 tactagagtt gacggctagc tcagtcctag gtacagtgct agctactaga gacatccgcc 301 tgctcaacat cgttttagag ctagaaatag caggttaaaa taaggctagt ccgttatcaa 361 cttgaaaaag tggcaccgag tcggtgcttt ttttgagtgg agagacagcg gaagcgactc 421 gtctcaaacg gacagctcgt agaaggtata cacgtcggaa gaatcgtatt tgttatctac 481 aggagatttt ttcaaatgag atggcgaaag tagatgatag tttctttcat cgacttgaag 541 agtctttttt ggtggaagaa gacaagaagc atgaacgtca tcctattttt ggaaatatag 601 tagatgaagt tgcttatcat gagaaatatc caactatcta tcatctgcga aaaaaattgg 661 tagattctac tgataaagcg gatttgcgct taatctattt ggccttagcg catatgatta 721 agtttcgtgg tcattttttg attgagggag atttaaatcc tgataatagt gatgtggaca 781 aactatttat ccagttggta caaacctaca atcaattatt tgaagaaaac cctattaacg 841 caagtggagt agatgctaaa gcgattcttt ctgcacgatt gagtaaatca agacgattag 901 aaaatctcat tgctcagctc cccggtgaga agaaaaatgg cttatttggg aatctcattg 961 ctttgtcatt gggtttgacc cctaatttta aatcaaattt tgatttggca gaagatgcta 1021 aattacagct ttcaaaagat acttacgatg atgatttaga taatttattg gcgcaaattg 1081 gagatcaata tgctgatttg tttttggcag ctaagaattt atcagatgct attttacttt 1141 cagatatcct aagagtaaat actgaaataa ctaaggctcc cctatcagct tcaatgatta 1201 aacgctacga tgaacatcat caagacttga ctcttttaaa agctttagtt cgacaacaac 1261 ttccagaaaa gtataaagaa atcttttttg atcaatcaaa aaacggatat gcaggttata 1321 ttgatggggg agctagccaa gaagaatttt ataaatttat caaaccaatt ttagaaaaaa 1381 tggatggtac tgaggaatta ttggtgaaac taaatcgtga agatttgctg cgcaagcaac 1441 ggacctttga caacggctct attccccatc aaattcactt gggtgagctg catgctattt 1501 tgagaagaca agaagacttt tatccatttt taaaagacaa tcgtgagaag attgaaaaaa 1561 tcttgacttt tcgaatccct tattatgttg gtccattggc gcgtggcaat agtcgttttg 1621 catggatgac tcggaagtct gaagaaacaa ttaccccatg gaattttgaa gaagttgtcg 1681 ataaaggtgc ttcagctcaa tcatttattg aacgcatgac aaactttgat aaaaatcttc 1741 caaatgaaaa agtactacca aaacatagtt tgctttatga gtattttacg gtttataacg 1801 aattgacaaa ggtcaaatat gttactgaag gaatgcgaaa accagcattt ctttcaggtg 1861 aacagaagaa agccattgtt gatttactct tcaaaacaaa tcgaaaagta accgttaagc 1921 aattaaaaga agattatttc aaaaaaatag aatgttttga tagtgttgaa atttcaggag 1981 ttgaagatag atttaatgct tcattaggta cctaccatga tttgctaaaa attattaaag 2041 ataaagattt tttggataat gaagaaaatg aagatatctt agaggatatt gttttaacat 2101 tgaccttatt tgaagatagg gagatgattg aggaaagact taaaacatat gctcacctct 2161 ttgatgataa ggtgatgaaa cagcttaaac gtcgccgtta tactggttgg ggacgtttgt 2221 ctcgaaaatt gattaatggt attagggata agcaatctgg caaaacaata ttagattttt 2281 tgaaatcaga tggttttgcc aatcgcaatt ttatgcagct gatccatgat gatagtttga 2341 catttaaaga agacattcaa aaagcacaag tgtctggaca aggcgatagt ttacatgaac 2401 atattgcaaa tttagctggt agccctgcta ttaaaaaagg tattttacag actgtaaaag 2461 ttgttgatga attggtcaaa gtaatggggc ggcataagcc agaaaatatc gttattgaaa 2521 tggcacgtga aaatcagaca actcaaaagg gccagaaaaa ttcgcgagag cgtatgaaac 2581 gaatcgaaga aggtatcaaa gaattaggaa gtcagattct taaagagcat cctgttgaaa 2641 atactcaatt gcaaaatgaa aagctctatc tctattatct ccaaaatgga agagacatgt 2701 atgtggacca agaattagat attaatcgtt taagtgatta tgatgtcgat gccattgttc 2761 cacaaagttt ccttaaagac gattcaatag acaataaggt cttaacgcgt tctgataaaa 2821 atcgtggtaa atcggataac gttccaagtg aagaagtagt caaaaagatg aaaaactatt 2881 ggagacaact tctaaacgcc aagttaatca ctcaacgtaa gtttgataat ttaacgaaag 2941 ctgaacgtgg aggtttgagt gaacttgata aagctggttt tatcaaacgc caattggttg 3001 aaactcgcca aatcactaag catgtggcac aaattttgga tagtcgcatg aatactaaat 3061 acgatgaaaa tgataaactt attcgagagg ttaaagtgat taccttaaaa tctaaattag 3121 tttctgactt ccgaaaagat ttccaattct ataaagtacg tgagattaac aattaccatc 3181 atgcccatga tgcgtatcta aatgccgtcg ttggaactgc tttgattaag aaatatccaa 3241 aacttgaatc ggagtttgtc tatggtgatt ataaagttta tgatgttcgt aaaatgattg 3301 ctaagtctga gcaagaaata ggcaaagcaa ccgcaaaata tttcttttac tctaatatca 3361 tgaacttctt caaaacagaa attacacttg caaatggaga gattcgcaaa cgccctctaa 3421 tcgaaactaa tggggaaact ggagaaattg tctgggataa agggcgagat tttgccacag 3481 tgcgcaaagt attgtccatg ccccaagtca atattgtcaa gaaaacagaa gtacagacag 3541 gcggattctc caaggagtca attttaccaa aaagaaattc ggacaagctt attgctcgta 3601 aaaaagactg ggatccaaaa aaatatggtg gttttgatag tccaacggta gcttattcag 3661 tcctagtggt tgctaaggtg gaaaaaggga aatcgaagaa gttaaaatcc gttaaagagt 3721 tactagggat cacaattatg gaaagaagtt cctttgaaaa aaatccgatt gactttttag 3781 aagctaaagg atataaggaa gttaaaaaag acttaatcat taaactacct aaatatagtc 3841 tttttgagtt agaaaacggt cgtaaacgga tgctggctag tgccggagaa ttacaaaaag 3901 gaaatgagct ggctctgcca agcaaatatg tgaatttttt atatttagct agtcattatg 3961 aaaagttgaa gggtagtcca gaagataacg aacaaaaaca attgtttgtg gagcagcata 4021 agcattattt agatgagatt attgagcaaa tcagtgaatt ttctaagcgt gttattttag 4081 cagatgccaa tttagataaa gttcttagtg catataacaa acatagagac aaaccaatac 4141 gtgaacaagc agaaaatatt attcatttat ttacgttgac gaatcttgga gctcccgctg 4201 cttttaaata ttttgataca acaattgatc gtaaacgata tacgtctaca aaagaagttt 4261 tagatgccac tcttatccat caatccatca ctggtcttta tgaaacacgc attgatttga 4321 gtcagctagg aggtgac //