BBa_K1207000 1 BBa_K1207000 Gram positive origin of replication 2013-09-11T11:00:00Z 2015-05-08T01:09:40Z This part was cloned from the plasmid pIKM1. Its original source was B. subtilis. This part is an origin of replication for Gram positive organisms. This sequence has been used in plasmids for electrotransformation of Clostridia with success. It is necessary to have a functional origin of replication for the chassis organism; therefore, if the chassis organism used is a Gram positive, this sequence will be a necessary addition to the iGEM standard plasmid backbones in order to allow for the replication of the plasmid. false false _1521_1241_ 0 14319 9 It's complicated false The cloning of this part was relatively straight-forward. No special considerations were required. false Jeremiah Miller BBa_K1207001 1 BBa_K1207001 Macrolide-Lincosamide-Streptogramin B Resistance Marker 2013-09-11T11:00:00Z 2016-01-28T01:51:46Z This part was cloned from the plasmid pIKM1. This mlsR gene was chosen as an additional selection marker for transformation of Gram positive chassis organisms. In the intended chassis organism of Clostridium acetobutylicum, the concentrations for selectivity were known, making this a convenient choice. false false _1521_1241_ 4206 14319 9 It's complicated false The cloning of this part was relatively straight-forward. No special design considerations were required. false Jeremiah Miller BBa_K1558000 1 BBa_K1558000 Clostridial shuttle vector 2014-10-02T11:00:00Z 2015-05-08T01:10:53Z Clostridium acetobutylicum Clostridial shuttle vector has a clostridial origin of replication, a promoter with out a ribosome binding site, erythromycin resistance, and a terminator ___ from the registry. false false _1943_ 0 14908 9 It's complicated false For use in anaerobic, Gram positive chassis. false Erin Weese component2393278 1 BBa_K1207001 component2393279 1 BBa_K1207000 annotation2393278 1 BBa_K1207001 range2393278 1 1 735 annotation2393279 1 BBa_K1207000 range2393279 1 744 1434 BBa_K1207000_sequence 1 cctgatatgctccgacgctttatatagaaaagaagattcaactaggtaaaatcttaatataggttgagatgataaggtttataaggaatttgtttgttctaatttttcactcattttgttctaatttcttttaacaaatgttcttttttttttagaacagttatgatatagttagaatagtttaaaataaggagtgagaaaaagatgaaagaaagatatggaacagtctataaaggctctcagaggctcatagacgaagaaagtggagaagtcatagaggtagacaagttataccgtaaacaaacgtctggtaacttcgtaaaggcatatatagtgcaattaataagtatgttagatatgattggcggaaaaaaacttaaaatcgttaactatatcctagataatgtccacttaagtaacaatacaatgatagctacaacaagagaaatagcaaaagctacaggaacaagtctacaaacagtaataacaacacttaaaatcttagaagaaggaaatattataaaaagaaaaactggagtattaatgttaaaccctgaactactaatgagaggcgacgaccaaaaacaaaaatacctcttactcgaatttgggaactttgagcaagaggcaaatgaaatagattgacctcccaataacaccacgtagttattgggaggtcaatctatgaaat BBa_K1558000_sequence 1 atgaacgagaaaaatataaaacacagtcaaaactttattacttcaaaacataatatagataaaataatgacaaatataagattaaatgaacatgataatatctttgaaatcggctcaggaaaagggcattttacccttgaattagtacagaggtgtaatttcgtaactgccattgaaatagaccataaattatgcaaaactacagaaaataaacttgttgatcacgataatttccaagttttaaacaaggatatattgcagtttaaatttcctaaaaaccaatcctataaaatatttggtaatataccttataacataagtacggatataatacgcaaaattgtttttgatagtatagctgatgagatttatttaatcgtggaatacgggtttgctaaaagattattaaatacaaaacgctcattggcattatttttaatggcagaagttgatatttctatattaagtatggttccaagagaatattttcatcctaaacctaaagtgaatagctcacttatcagattaaatagaaaaaaatcaagaatatcacacaaagataaacagaagtataattatttcgttatgaaatgggttaacaaagaatacaagaaaatatttacaaaaaatcaatttaacaattccttaaaacatgcaggaattgacgatttaaacaatattagctttgaacaattcttatctcttttcaatagctataaattatttaataagtaatactagagcctgatatgctccgacgctttatatagaaaagaagattcaactaggtaaaatcttaatataggttgagatgataaggtttataaggaatttgtttgttctaatttttcactcattttgttctaatttcttttaacaaatgttcttttttttttagaacagttatgatatagttagaatagtttaaaataaggagtgagaaaaagatgaaagaaagatatggaacagtctataaaggctctcagaggctcatagacgaagaaagtggagaagtcatagaggtagacaagttataccgtaaacaaacgtctggtaacttcgtaaaggcatatatagtgcaattaataagtatgttagatatgattggcggaaaaaaacttaaaatcgttaactatatcctagataatgtccacttaagtaacaatacaatgatagctacaacaagagaaatagcaaaagctacaggaacaagtctacaaacagtaataacaacacttaaaatcttagaagaaggaaatattataaaaagaaaaactggagtattaatgttaaaccctgaactactaatgagaggcgacgaccaaaaacaaaaatacctcttactcgaatttgggaactttgagcaagaggcaaatgaaatagattgacctcccaataacaccacgtagttattgggaggtcaatctatgaaat BBa_K1207001_sequence 1 atgaacgagaaaaatataaaacacagtcaaaactttattacttcaaaacataatatagataaaataatgacaaatataagattaaatgaacatgataatatctttgaaatcggctcaggaaaagggcattttacccttgaattagtacagaggtgtaatttcgtaactgccattgaaatagaccataaattatgcaaaactacagaaaataaacttgttgatcacgataatttccaagttttaaacaaggatatattgcagtttaaatttcctaaaaaccaatcctataaaatatttggtaatataccttataacataagtacggatataatacgcaaaattgtttttgatagtatagctgatgagatttatttaatcgtggaatacgggtttgctaaaagattattaaatacaaaacgctcattggcattatttttaatggcagaagttgatatttctatattaagtatggttccaagagaatattttcatcctaaacctaaagtgaatagctcacttatcagattaaatagaaaaaaatcaagaatatcacacaaagataaacagaagtataattatttcgttatgaaatgggttaacaaagaatacaagaaaatatttacaaaaaatcaatttaacaattccttaaaacatgcaggaattgacgatttaaacaatattagctttgaacaattcttatctcttttcaatagctataaattatttaataagtaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z