LOCUS BBa_K1559004 5127 bp DNA linear 22-Dec-2024 DEFINITION CRISPR/Cas9 system with Anderson medium-expression constitutive promoter ACCESSION BBa_K1559004 VERSION BBa_K1559004.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23108 misc_feature 40..43 /label=conserved RBS 36..47 /label=BBa_B0034 CDS 48..4154 /label=cas9 misc_feature 4307..4342 /label=direct repeat misc_feature 4343..4372 /label=spacer placeholder containing BsaI sites misc_feature 4373..4408 /label=direct repeat misc_feature 4307..4408 /label=crRNA array misc_feature 4747..4917 /label=tracrRNA misc_feature 4918..5127 /label=Region containing bidirectional constitutive promoter stem_loop 4675..4721 /label=predicted terminator rev stem_loop 4675..4723 /label=predicted terminator fwd stem_loop 4739..4780 /label=stem loop within tracrRNA CDS 48..5127 /label=BBa_K1559002 ORIGIN 1 ctgacagcta gctcagtcct aggtataatg ctagcaaaga ggagaaaatg gataagaaat 61 actcaatagg cttagatatc ggcacaaata gcgtcggatg ggcggtgatc actgatgaat 121 ataaggttcc gtctaaaaag ttcaaggttc tgggaaatac agaccgccac agtatcaaaa 181 aaaatcttat aggggctctt ttatttgaca gtggagagac agcggaagcg actcgtctca 241 aacggacagc tcgtagaagg tatacacgtc ggaagaatcg tatttgttat ctacaggaga 301 ttttttcaaa tgagatggcg aaagtagatg atagtttctt tcatcgactt gaagagtctt 361 ttttggtgga agaagacaag aagcatgaac gtcatcctat ttttggaaat atagtagatg 421 aagttgctta tcatgagaaa tatccaacta tctatcatct gcgaaaaaaa ttggtagatt 481 ctactgataa agcggatttg cgcttaatct atttggcctt agcgcatatg attaagtttc 541 gtggtcattt tttgattgag ggagatttaa atcctgataa tagtgatgtg gacaaactat 601 ttatccagtt ggtacaaacc tacaatcaat tatttgaaga aaaccctatt aacgcaagtg 661 gagtagatgc taaagcgatt ctttctgcac gattgagtaa atcaagacga ttagaaaatc 721 tcattgctca gctccccggt gagaagaaaa atggcttatt tgggaatctc attgctttgt 781 cattgggttt gacccctaat tttaaatcaa attttgattt ggcagaagat gctaaattac 841 agctttcaaa agatacttac gatgatgatt tagataattt attggcgcaa attggagatc 901 aatatgctga tttgtttttg gcagctaaga atttatcaga tgctatttta ctttcagata 961 tcctaagagt aaatactgaa ataactaagg ctcccctatc agcttcaatg attaaacgct 1021 acgatgaaca tcatcaagac ttgactcttt taaaagcttt agttcgacaa caacttccag 1081 aaaagtataa agaaatcttt tttgatcaat caaaaaacgg atatgcaggt tatattgatg 1141 ggggagctag ccaagaagaa ttttataaat ttatcaaacc aattttagaa aaaatggatg 1201 gtactgagga attattggtg aaactaaatc gtgaagattt gctgcgcaag caacggacct 1261 ttgacaacgg ctctattccc catcaaattc acttgggtga gctgcatgct attttgagaa 1321 gacaagaaga cttttatcca tttttaaaag acaatcgtga gaagattgaa aaaatcttga 1381 cttttcgtat tccttattat gttggtccat tggcgcgtgg caatagtcgt tttgcatgga 1441 tgactcggaa gtctgaagaa acaattaccc catggaattt tgaagaagtt gtcgataaag 1501 gtgcttcagc tcaatcattt attgaacgca tgacaaactt tgataaaaat cttccaaatg 1561 aaaaagtact accaaaacat agtttgcttt atgagtattt tacggtttat aacgaattga 1621 caaaggtcaa atatgttact gaaggaatgc gaaaaccagc atttctttca ggtgaacaga 1681 agaaagccat tgttgattta ctcttcaaaa caaatcgaaa agtaaccgtt aagcaattaa 1741 aagaagatta tttcaaaaaa atagaatgtt ttgatagtgt tgaaatttca ggagttgaag 1801 atagatttaa tgcttcatta ggtacctacc atgatttgct aaaaattatt aaagataaag 1861 attttttgga taatgaagaa aatgaagata tcttagagga tattgtttta acattgacct 1921 tatttgaaga tagggagatg attgaggaaa gacttaaaac atatgctcac ctctttgatg 1981 ataaggtgat gaaacagctt aaacgtcgcc gttatactgg ttggggacgt ttgtctcgaa 2041 aattgattaa tggtattagg gataagcaat ctggcaaaac aatattagat tttttgaaat 2101 cagatggttt tgccaatcgc aattttatgc agctgatcca tgatgatagt ttgacattta 2161 aagaagacat tcaaaaagca caagtgtctg gacaaggcga tagtttacat gaacatattg 2221 caaatttagc tggtagccct gctattaaaa aaggtatttt acagactgta aaagttgttg 2281 atgaattggt caaagtaatg gggcggcata agccagaaaa tatcgttatt gaaatggcac 2341 gtgaaaatca gacaactcaa aagggccaga aaaattcgcg agagcgtatg aaacgaatcg 2401 aagaaggtat caaagaatta ggaagtcaga ttcttaaaga gcatcctgtt gaaaatactc 2461 aattgcaaaa tgaaaagctc tatctctatt atctccaaaa tggaagagac atgtatgtgg 2521 accaagaatt agatattaat cgtttaagtg attatgatgt cgatcacatt gttccacaaa 2581 gtttccttaa agacgattca atagacaata aggtcttaac gcgttctgat aaaaatcgtg 2641 gtaaatcgga taacgttcca agtgaagaag tagtcaaaaa gatgaaaaac tattggagac 2701 aacttctaaa cgccaagtta atcactcaac gtaagtttga taatttaacg aaagctgaac 2761 gtggaggttt gagtgaactt gataaagctg gttttatcaa acgccaattg gttgaaactc 2821 gccaaatcac taagcatgtg gcacaaattt tggatagtcg catgaatact aaatacgatg 2881 aaaatgataa acttattcga gaggttaaag tgattacctt aaaatctaaa ttagtttctg 2941 acttccgaaa agatttccaa ttctataaag tacgtgagat taacaattac catcatgccc 3001 atgatgcgta tctaaatgcc gtcgttggaa ctgctttgat taagaaatat ccaaaacttg 3061 aatcggagtt tgtctatggt gattataaag tttatgatgt tcgtaaaatg attgctaagt 3121 ctgagcaaga aataggcaaa gcaaccgcaa aatatttctt ttactctaat atcatgaact 3181 tcttcaaaac agaaattaca cttgcaaatg gagagattcg caaacgccct ctaatcgaaa 3241 ctaatgggga aactggagaa attgtctggg ataaagggcg agattttgcc acagtgcgca 3301 aagtattgtc catgccccaa gtcaatattg tcaagaaaac agaagtacag acaggcggat 3361 tctccaagga gtcaatttta ccaaaaagaa attcggacaa gcttattgct cgtaaaaaag 3421 actgggatcc aaaaaaatat ggtggttttg atagtccaac ggtagcttat tcagtcctag 3481 tggttgctaa ggtggaaaaa gggaaatcga agaagttaaa atccgttaaa gagttactag 3541 ggatcacaat tatggaaaga agttcctttg aaaaaaatcc gattgacttt ttagaagcta 3601 aaggatataa ggaagttaaa aaagacttaa tcattaaact acctaaatat agtctttttg 3661 agttagaaaa cggtcgtaaa cggatgctgg ctagtgccgg agaattacaa aaaggaaatg 3721 agctggctct gccaagcaaa tatgtgaatt ttttatattt agctagtcat tatgaaaagt 3781 tgaagggtag tccagaagat aacgaacaaa aacaattgtt tgtggagcag cataagcatt 3841 atttagatga gattattgag caaatcagtg aattttctaa gcgtgttatt ttagcagatg 3901 ccaatttaga taaagttctt agtgcatata acaaacatag agacaaacca atacgtgaac 3961 aagcagaaaa tattattcat ttatttacgt tgacgaatct tggagctccc gctgctttta 4021 aatattttga tacaacaatt gatcgtaaac gatatacgtc tacaaaagaa gttttagatg 4081 ccactcttat ccatcaatcc atcactggtc tttatgaaac acgcattgat ttgagtcagc 4141 taggaggtga ctgaagtata ttttagatga agattatttc ttaataacta aaaatatggt 4201 ataatactct taataaatgc agtaatacag gggcttttca agactgaagt ctagctgaga 4261 caaatagtgc gattacgaaa ttttttagac aaaaatagtc tacgaggttt tagagctatg 4321 ctgttttgaa tggtcccaaa actgagacca gtctcggaag ctcaaaggtc tcgttttaga 4381 gctatgctgt tttgaatggt cccaaaactt cagcacactg agacttgttg agttccatgt 4441 tttagagcta tgctgttttg aatggactcc attcaacatt gccgatgata acttgagaaa 4501 gagggttaat accagcagtc ggataccttc ctattctttc tgttaaagcg ttttcatgtt 4561 ataataggca aaagaagagt agtgttacga aatcatcctg tggagcttag taggtttagc 4621 aagatggcag cgcctaaatg tagaatgata aaaggattaa gagattaatt tccctaaaaa 4681 tgataaaaca agcgttttga aagcgcttgt ttttttggtt tgcagtcaga gtagaataga 4741 agtatcaaaa aaagcaccga ctcggtgcca ctttttcaag ttgataacgg actagcctta 4801 ttttaacttg ctatgctgtt ttgaatggtt ccaacaagat tattttataa cttttataac 4861 aaataatcaa ggagaaattc aaagaaattt atcagccata aaacaatact taatactata 4921 gaatgataac aaaataaact actttttaaa agaattttgt gttataatct atttattatt 4981 aagtattggg taatattttt tgaagagata ttttgaaaaa gaaaaattaa agcatattaa 5041 actaatttcg gaggtcatta aaactattat tgaaatcatc aaactcatta tggatttaat 5101 ttaaactttt tattttagga ggcaaaa //