LOCUS BBa_K1559006 5222 bp DNA linear 29-Nov-2024 DEFINITION CRISPR/Cas9 system with pBAD inducible promoter ACCESSION BBa_K1559006 VERSION BBa_K1559006.1 FEATURES Location/Qualifiers promoter 1..130 /label=BBa_I13453 misc_feature 135..138 /label=conserved RBS 131..142 /label=BBa_B0034 CDS 143..4249 /label=cas9 misc_feature 4402..4437 /label=direct repeat misc_feature 4438..4467 /label=spacer placeholder containing BsaI sites misc_feature 4468..4503 /label=direct repeat misc_feature 4402..4503 /label=crRNA array misc_feature 4842..5012 /label=tracrRNA misc_feature 5013..5222 /label=Region containing bidirectional constitutive promoter stem_loop 4770..4816 /label=predicted terminator rev stem_loop 4770..4818 /label=predicted terminator fwd stem_loop 4834..4875 /label=stem loop within tracrRNA CDS 143..5222 /label=BBa_K1559002 ORIGIN 1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat 61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt 121 ttgggctagc aaagaggaga aaatggataa gaaatactca ataggcttag atatcggcac 181 aaatagcgtc ggatgggcgg tgatcactga tgaatataag gttccgtcta aaaagttcaa 241 ggttctggga aatacagacc gccacagtat caaaaaaaat cttatagggg ctcttttatt 301 tgacagtgga gagacagcgg aagcgactcg tctcaaacgg acagctcgta gaaggtatac 361 acgtcggaag aatcgtattt gttatctaca ggagattttt tcaaatgaga tggcgaaagt 421 agatgatagt ttctttcatc gacttgaaga gtcttttttg gtggaagaag acaagaagca 481 tgaacgtcat cctatttttg gaaatatagt agatgaagtt gcttatcatg agaaatatcc 541 aactatctat catctgcgaa aaaaattggt agattctact gataaagcgg atttgcgctt 601 aatctatttg gccttagcgc atatgattaa gtttcgtggt cattttttga ttgagggaga 661 tttaaatcct gataatagtg atgtggacaa actatttatc cagttggtac aaacctacaa 721 tcaattattt gaagaaaacc ctattaacgc aagtggagta gatgctaaag cgattctttc 781 tgcacgattg agtaaatcaa gacgattaga aaatctcatt gctcagctcc ccggtgagaa 841 gaaaaatggc ttatttggga atctcattgc tttgtcattg ggtttgaccc ctaattttaa 901 atcaaatttt gatttggcag aagatgctaa attacagctt tcaaaagata cttacgatga 961 tgatttagat aatttattgg cgcaaattgg agatcaatat gctgatttgt ttttggcagc 1021 taagaattta tcagatgcta ttttactttc agatatccta agagtaaata ctgaaataac 1081 taaggctccc ctatcagctt caatgattaa acgctacgat gaacatcatc aagacttgac 1141 tcttttaaaa gctttagttc gacaacaact tccagaaaag tataaagaaa tcttttttga 1201 tcaatcaaaa aacggatatg caggttatat tgatggggga gctagccaag aagaatttta 1261 taaatttatc aaaccaattt tagaaaaaat ggatggtact gaggaattat tggtgaaact 1321 aaatcgtgaa gatttgctgc gcaagcaacg gacctttgac aacggctcta ttccccatca 1381 aattcacttg ggtgagctgc atgctatttt gagaagacaa gaagactttt atccattttt 1441 aaaagacaat cgtgagaaga ttgaaaaaat cttgactttt cgtattcctt attatgttgg 1501 tccattggcg cgtggcaata gtcgttttgc atggatgact cggaagtctg aagaaacaat 1561 taccccatgg aattttgaag aagttgtcga taaaggtgct tcagctcaat catttattga 1621 acgcatgaca aactttgata aaaatcttcc aaatgaaaaa gtactaccaa aacatagttt 1681 gctttatgag tattttacgg tttataacga attgacaaag gtcaaatatg ttactgaagg 1741 aatgcgaaaa ccagcatttc tttcaggtga acagaagaaa gccattgttg atttactctt 1801 caaaacaaat cgaaaagtaa ccgttaagca attaaaagaa gattatttca aaaaaataga 1861 atgttttgat agtgttgaaa tttcaggagt tgaagataga tttaatgctt cattaggtac 1921 ctaccatgat ttgctaaaaa ttattaaaga taaagatttt ttggataatg aagaaaatga 1981 agatatctta gaggatattg ttttaacatt gaccttattt gaagataggg agatgattga 2041 ggaaagactt aaaacatatg ctcacctctt tgatgataag gtgatgaaac agcttaaacg 2101 tcgccgttat actggttggg gacgtttgtc tcgaaaattg attaatggta ttagggataa 2161 gcaatctggc aaaacaatat tagatttttt gaaatcagat ggttttgcca atcgcaattt 2221 tatgcagctg atccatgatg atagtttgac atttaaagaa gacattcaaa aagcacaagt 2281 gtctggacaa ggcgatagtt tacatgaaca tattgcaaat ttagctggta gccctgctat 2341 taaaaaaggt attttacaga ctgtaaaagt tgttgatgaa ttggtcaaag taatggggcg 2401 gcataagcca gaaaatatcg ttattgaaat ggcacgtgaa aatcagacaa ctcaaaaggg 2461 ccagaaaaat tcgcgagagc gtatgaaacg aatcgaagaa ggtatcaaag aattaggaag 2521 tcagattctt aaagagcatc ctgttgaaaa tactcaattg caaaatgaaa agctctatct 2581 ctattatctc caaaatggaa gagacatgta tgtggaccaa gaattagata ttaatcgttt 2641 aagtgattat gatgtcgatc acattgttcc acaaagtttc cttaaagacg attcaataga 2701 caataaggtc ttaacgcgtt ctgataaaaa tcgtggtaaa tcggataacg ttccaagtga 2761 agaagtagtc aaaaagatga aaaactattg gagacaactt ctaaacgcca agttaatcac 2821 tcaacgtaag tttgataatt taacgaaagc tgaacgtgga ggtttgagtg aacttgataa 2881 agctggtttt atcaaacgcc aattggttga aactcgccaa atcactaagc atgtggcaca 2941 aattttggat agtcgcatga atactaaata cgatgaaaat gataaactta ttcgagaggt 3001 taaagtgatt accttaaaat ctaaattagt ttctgacttc cgaaaagatt tccaattcta 3061 taaagtacgt gagattaaca attaccatca tgcccatgat gcgtatctaa atgccgtcgt 3121 tggaactgct ttgattaaga aatatccaaa acttgaatcg gagtttgtct atggtgatta 3181 taaagtttat gatgttcgta aaatgattgc taagtctgag caagaaatag gcaaagcaac 3241 cgcaaaatat ttcttttact ctaatatcat gaacttcttc aaaacagaaa ttacacttgc 3301 aaatggagag attcgcaaac gccctctaat cgaaactaat ggggaaactg gagaaattgt 3361 ctgggataaa gggcgagatt ttgccacagt gcgcaaagta ttgtccatgc cccaagtcaa 3421 tattgtcaag aaaacagaag tacagacagg cggattctcc aaggagtcaa ttttaccaaa 3481 aagaaattcg gacaagctta ttgctcgtaa aaaagactgg gatccaaaaa aatatggtgg 3541 ttttgatagt ccaacggtag cttattcagt cctagtggtt gctaaggtgg aaaaagggaa 3601 atcgaagaag ttaaaatccg ttaaagagtt actagggatc acaattatgg aaagaagttc 3661 ctttgaaaaa aatccgattg actttttaga agctaaagga tataaggaag ttaaaaaaga 3721 cttaatcatt aaactaccta aatatagtct ttttgagtta gaaaacggtc gtaaacggat 3781 gctggctagt gccggagaat tacaaaaagg aaatgagctg gctctgccaa gcaaatatgt 3841 gaatttttta tatttagcta gtcattatga aaagttgaag ggtagtccag aagataacga 3901 acaaaaacaa ttgtttgtgg agcagcataa gcattattta gatgagatta ttgagcaaat 3961 cagtgaattt tctaagcgtg ttattttagc agatgccaat ttagataaag ttcttagtgc 4021 atataacaaa catagagaca aaccaatacg tgaacaagca gaaaatatta ttcatttatt 4081 tacgttgacg aatcttggag ctcccgctgc ttttaaatat tttgatacaa caattgatcg 4141 taaacgatat acgtctacaa aagaagtttt agatgccact cttatccatc aatccatcac 4201 tggtctttat gaaacacgca ttgatttgag tcagctagga ggtgactgaa gtatatttta 4261 gatgaagatt atttcttaat aactaaaaat atggtataat actcttaata aatgcagtaa 4321 tacaggggct tttcaagact gaagtctagc tgagacaaat agtgcgatta cgaaattttt 4381 tagacaaaaa tagtctacga ggttttagag ctatgctgtt ttgaatggtc ccaaaactga 4441 gaccagtctc ggaagctcaa aggtctcgtt ttagagctat gctgttttga atggtcccaa 4501 aacttcagca cactgagact tgttgagttc catgttttag agctatgctg ttttgaatgg 4561 actccattca acattgccga tgataacttg agaaagaggg ttaataccag cagtcggata 4621 ccttcctatt ctttctgtta aagcgttttc atgttataat aggcaaaaga agagtagtgt 4681 tacgaaatca tcctgtggag cttagtaggt ttagcaagat ggcagcgcct aaatgtagaa 4741 tgataaaagg attaagagat taatttccct aaaaatgata aaacaagcgt tttgaaagcg 4801 cttgtttttt tggtttgcag tcagagtaga atagaagtat caaaaaaagc accgactcgg 4861 tgccactttt tcaagttgat aacggactag ccttatttta acttgctatg ctgttttgaa 4921 tggttccaac aagattattt tataactttt ataacaaata atcaaggaga aattcaaaga 4981 aatttatcag ccataaaaca atacttaata ctatagaatg ataacaaaat aaactacttt 5041 ttaaaagaat tttgtgttat aatctattta ttattaagta ttgggtaata ttttttgaag 5101 agatattttg aaaaagaaaa attaaagcat attaaactaa tttcggaggt cattaaaact 5161 attattgaaa tcatcaaact cattatggat ttaatttaaa ctttttattt taggaggcaa 5221 aa //