LOCUS BBa_K1559007 5147 bp DNA linear 22-Nov-2024 DEFINITION CRISPR/Cas9 system with pLac inducible promoter ACCESSION BBa_K1559007 VERSION BBa_K1559007.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 60..63 /label=conserved RBS 56..67 /label=BBa_B0034 CDS 68..4174 /label=cas9 misc_feature 4327..4362 /label=direct repeat misc_feature 4363..4392 /label=spacer placeholder containing BsaI sites misc_feature 4393..4428 /label=direct repeat misc_feature 4327..4428 /label=crRNA array misc_feature 4767..4937 /label=tracrRNA misc_feature 4938..5147 /label=Region containing bidirectional constitutive promoter stem_loop 4695..4741 /label=predicted terminator rev stem_loop 4695..4743 /label=predicted terminator fwd stem_loop 4759..4800 /label=stem loop within tracrRNA CDS 68..5147 /label=BBa_K1559002 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacaaaaga 61 ggagaaaatg gataagaaat actcaatagg cttagatatc ggcacaaata gcgtcggatg 121 ggcggtgatc actgatgaat ataaggttcc gtctaaaaag ttcaaggttc tgggaaatac 181 agaccgccac agtatcaaaa aaaatcttat aggggctctt ttatttgaca gtggagagac 241 agcggaagcg actcgtctca aacggacagc tcgtagaagg tatacacgtc ggaagaatcg 301 tatttgttat ctacaggaga ttttttcaaa tgagatggcg aaagtagatg atagtttctt 361 tcatcgactt gaagagtctt ttttggtgga agaagacaag aagcatgaac gtcatcctat 421 ttttggaaat atagtagatg aagttgctta tcatgagaaa tatccaacta tctatcatct 481 gcgaaaaaaa ttggtagatt ctactgataa agcggatttg cgcttaatct atttggcctt 541 agcgcatatg attaagtttc gtggtcattt tttgattgag ggagatttaa atcctgataa 601 tagtgatgtg gacaaactat ttatccagtt ggtacaaacc tacaatcaat tatttgaaga 661 aaaccctatt aacgcaagtg gagtagatgc taaagcgatt ctttctgcac gattgagtaa 721 atcaagacga ttagaaaatc tcattgctca gctccccggt gagaagaaaa atggcttatt 781 tgggaatctc attgctttgt cattgggttt gacccctaat tttaaatcaa attttgattt 841 ggcagaagat gctaaattac agctttcaaa agatacttac gatgatgatt tagataattt 901 attggcgcaa attggagatc aatatgctga tttgtttttg gcagctaaga atttatcaga 961 tgctatttta ctttcagata tcctaagagt aaatactgaa ataactaagg ctcccctatc 1021 agcttcaatg attaaacgct acgatgaaca tcatcaagac ttgactcttt taaaagcttt 1081 agttcgacaa caacttccag aaaagtataa agaaatcttt tttgatcaat caaaaaacgg 1141 atatgcaggt tatattgatg ggggagctag ccaagaagaa ttttataaat ttatcaaacc 1201 aattttagaa aaaatggatg gtactgagga attattggtg aaactaaatc gtgaagattt 1261 gctgcgcaag caacggacct ttgacaacgg ctctattccc catcaaattc acttgggtga 1321 gctgcatgct attttgagaa gacaagaaga cttttatcca tttttaaaag acaatcgtga 1381 gaagattgaa aaaatcttga cttttcgtat tccttattat gttggtccat tggcgcgtgg 1441 caatagtcgt tttgcatgga tgactcggaa gtctgaagaa acaattaccc catggaattt 1501 tgaagaagtt gtcgataaag gtgcttcagc tcaatcattt attgaacgca tgacaaactt 1561 tgataaaaat cttccaaatg aaaaagtact accaaaacat agtttgcttt atgagtattt 1621 tacggtttat aacgaattga caaaggtcaa atatgttact gaaggaatgc gaaaaccagc 1681 atttctttca ggtgaacaga agaaagccat tgttgattta ctcttcaaaa caaatcgaaa 1741 agtaaccgtt aagcaattaa aagaagatta tttcaaaaaa atagaatgtt ttgatagtgt 1801 tgaaatttca ggagttgaag atagatttaa tgcttcatta ggtacctacc atgatttgct 1861 aaaaattatt aaagataaag attttttgga taatgaagaa aatgaagata tcttagagga 1921 tattgtttta acattgacct tatttgaaga tagggagatg attgaggaaa gacttaaaac 1981 atatgctcac ctctttgatg ataaggtgat gaaacagctt aaacgtcgcc gttatactgg 2041 ttggggacgt ttgtctcgaa aattgattaa tggtattagg gataagcaat ctggcaaaac 2101 aatattagat tttttgaaat cagatggttt tgccaatcgc aattttatgc agctgatcca 2161 tgatgatagt ttgacattta aagaagacat tcaaaaagca caagtgtctg gacaaggcga 2221 tagtttacat gaacatattg caaatttagc tggtagccct gctattaaaa aaggtatttt 2281 acagactgta aaagttgttg atgaattggt caaagtaatg gggcggcata agccagaaaa 2341 tatcgttatt gaaatggcac gtgaaaatca gacaactcaa aagggccaga aaaattcgcg 2401 agagcgtatg aaacgaatcg aagaaggtat caaagaatta ggaagtcaga ttcttaaaga 2461 gcatcctgtt gaaaatactc aattgcaaaa tgaaaagctc tatctctatt atctccaaaa 2521 tggaagagac atgtatgtgg accaagaatt agatattaat cgtttaagtg attatgatgt 2581 cgatcacatt gttccacaaa gtttccttaa agacgattca atagacaata aggtcttaac 2641 gcgttctgat aaaaatcgtg gtaaatcgga taacgttcca agtgaagaag tagtcaaaaa 2701 gatgaaaaac tattggagac aacttctaaa cgccaagtta atcactcaac gtaagtttga 2761 taatttaacg aaagctgaac gtggaggttt gagtgaactt gataaagctg gttttatcaa 2821 acgccaattg gttgaaactc gccaaatcac taagcatgtg gcacaaattt tggatagtcg 2881 catgaatact aaatacgatg aaaatgataa acttattcga gaggttaaag tgattacctt 2941 aaaatctaaa ttagtttctg acttccgaaa agatttccaa ttctataaag tacgtgagat 3001 taacaattac catcatgccc atgatgcgta tctaaatgcc gtcgttggaa ctgctttgat 3061 taagaaatat ccaaaacttg aatcggagtt tgtctatggt gattataaag tttatgatgt 3121 tcgtaaaatg attgctaagt ctgagcaaga aataggcaaa gcaaccgcaa aatatttctt 3181 ttactctaat atcatgaact tcttcaaaac agaaattaca cttgcaaatg gagagattcg 3241 caaacgccct ctaatcgaaa ctaatgggga aactggagaa attgtctggg ataaagggcg 3301 agattttgcc acagtgcgca aagtattgtc catgccccaa gtcaatattg tcaagaaaac 3361 agaagtacag acaggcggat tctccaagga gtcaatttta ccaaaaagaa attcggacaa 3421 gcttattgct cgtaaaaaag actgggatcc aaaaaaatat ggtggttttg atagtccaac 3481 ggtagcttat tcagtcctag tggttgctaa ggtggaaaaa gggaaatcga agaagttaaa 3541 atccgttaaa gagttactag ggatcacaat tatggaaaga agttcctttg aaaaaaatcc 3601 gattgacttt ttagaagcta aaggatataa ggaagttaaa aaagacttaa tcattaaact 3661 acctaaatat agtctttttg agttagaaaa cggtcgtaaa cggatgctgg ctagtgccgg 3721 agaattacaa aaaggaaatg agctggctct gccaagcaaa tatgtgaatt ttttatattt 3781 agctagtcat tatgaaaagt tgaagggtag tccagaagat aacgaacaaa aacaattgtt 3841 tgtggagcag cataagcatt atttagatga gattattgag caaatcagtg aattttctaa 3901 gcgtgttatt ttagcagatg ccaatttaga taaagttctt agtgcatata acaaacatag 3961 agacaaacca atacgtgaac aagcagaaaa tattattcat ttatttacgt tgacgaatct 4021 tggagctccc gctgctttta aatattttga tacaacaatt gatcgtaaac gatatacgtc 4081 tacaaaagaa gttttagatg ccactcttat ccatcaatcc atcactggtc tttatgaaac 4141 acgcattgat ttgagtcagc taggaggtga ctgaagtata ttttagatga agattatttc 4201 ttaataacta aaaatatggt ataatactct taataaatgc agtaatacag gggcttttca 4261 agactgaagt ctagctgaga caaatagtgc gattacgaaa ttttttagac aaaaatagtc 4321 tacgaggttt tagagctatg ctgttttgaa tggtcccaaa actgagacca gtctcggaag 4381 ctcaaaggtc tcgttttaga gctatgctgt tttgaatggt cccaaaactt cagcacactg 4441 agacttgttg agttccatgt tttagagcta tgctgttttg aatggactcc attcaacatt 4501 gccgatgata acttgagaaa gagggttaat accagcagtc ggataccttc ctattctttc 4561 tgttaaagcg ttttcatgtt ataataggca aaagaagagt agtgttacga aatcatcctg 4621 tggagcttag taggtttagc aagatggcag cgcctaaatg tagaatgata aaaggattaa 4681 gagattaatt tccctaaaaa tgataaaaca agcgttttga aagcgcttgt ttttttggtt 4741 tgcagtcaga gtagaataga agtatcaaaa aaagcaccga ctcggtgcca ctttttcaag 4801 ttgataacgg actagcctta ttttaacttg ctatgctgtt ttgaatggtt ccaacaagat 4861 tattttataa cttttataac aaataatcaa ggagaaattc aaagaaattt atcagccata 4921 aaacaatact taatactata gaatgataac aaaataaact actttttaaa agaattttgt 4981 gttataatct atttattatt aagtattggg taatattttt tgaagagata ttttgaaaaa 5041 gaaaaattaa agcatattaa actaatttcg gaggtcatta aaactattat tgaaatcatc 5101 aaactcatta tggatttaat ttaaactttt tattttagga ggcaaaa //