LOCUS BBa_K1559008 5214 bp DNA linear 29-Nov-2024 DEFINITION CRISPR/Cas9 system with pRha inducible promoter ACCESSION BBa_K1559008 VERSION BBa_K1559008.1 FEATURES Location/Qualifiers promoter 1..122 /label=pRha promoter 1..122 /label=BBa_K914003 misc_feature 127..130 /label=conserved RBS 123..134 /label=BBa_B0034 CDS 135..4241 /label=cas9 misc_feature 4394..4429 /label=direct repeat misc_feature 4430..4459 /label=spacer placeholder containing BsaI sites misc_feature 4460..4495 /label=direct repeat misc_feature 4394..4495 /label=crRNA array misc_feature 4834..5004 /label=tracrRNA misc_feature 5005..5214 /label=Region containing bidirectional constitutive promoter stem_loop 4762..4808 /label=predicted terminator rev stem_loop 4762..4810 /label=predicted terminator fwd stem_loop 4826..4867 /label=stem loop within tracrRNA CDS 135..5214 /label=BBa_K1559002 ORIGIN 1 ccacaattca gcaaattgtg aacatcatca cgttcatctt tccctggttg ccaatggccc 61 attttcctgt cagtaacgag aaggtcgcgt attcaggcgc tttttagact ggtcgtaatg 121 aaaaagagga gaaaatggat aagaaatact caataggctt agatatcggc acaaatagcg 181 tcggatgggc ggtgatcact gatgaatata aggttccgtc taaaaagttc aaggttctgg 241 gaaatacaga ccgccacagt atcaaaaaaa atcttatagg ggctctttta tttgacagtg 301 gagagacagc ggaagcgact cgtctcaaac ggacagctcg tagaaggtat acacgtcgga 361 agaatcgtat ttgttatcta caggagattt tttcaaatga gatggcgaaa gtagatgata 421 gtttctttca tcgacttgaa gagtcttttt tggtggaaga agacaagaag catgaacgtc 481 atcctatttt tggaaatata gtagatgaag ttgcttatca tgagaaatat ccaactatct 541 atcatctgcg aaaaaaattg gtagattcta ctgataaagc ggatttgcgc ttaatctatt 601 tggccttagc gcatatgatt aagtttcgtg gtcatttttt gattgaggga gatttaaatc 661 ctgataatag tgatgtggac aaactattta tccagttggt acaaacctac aatcaattat 721 ttgaagaaaa ccctattaac gcaagtggag tagatgctaa agcgattctt tctgcacgat 781 tgagtaaatc aagacgatta gaaaatctca ttgctcagct ccccggtgag aagaaaaatg 841 gcttatttgg gaatctcatt gctttgtcat tgggtttgac ccctaatttt aaatcaaatt 901 ttgatttggc agaagatgct aaattacagc tttcaaaaga tacttacgat gatgatttag 961 ataatttatt ggcgcaaatt ggagatcaat atgctgattt gtttttggca gctaagaatt 1021 tatcagatgc tattttactt tcagatatcc taagagtaaa tactgaaata actaaggctc 1081 ccctatcagc ttcaatgatt aaacgctacg atgaacatca tcaagacttg actcttttaa 1141 aagctttagt tcgacaacaa cttccagaaa agtataaaga aatctttttt gatcaatcaa 1201 aaaacggata tgcaggttat attgatgggg gagctagcca agaagaattt tataaattta 1261 tcaaaccaat tttagaaaaa atggatggta ctgaggaatt attggtgaaa ctaaatcgtg 1321 aagatttgct gcgcaagcaa cggacctttg acaacggctc tattccccat caaattcact 1381 tgggtgagct gcatgctatt ttgagaagac aagaagactt ttatccattt ttaaaagaca 1441 atcgtgagaa gattgaaaaa atcttgactt ttcgtattcc ttattatgtt ggtccattgg 1501 cgcgtggcaa tagtcgtttt gcatggatga ctcggaagtc tgaagaaaca attaccccat 1561 ggaattttga agaagttgtc gataaaggtg cttcagctca atcatttatt gaacgcatga 1621 caaactttga taaaaatctt ccaaatgaaa aagtactacc aaaacatagt ttgctttatg 1681 agtattttac ggtttataac gaattgacaa aggtcaaata tgttactgaa ggaatgcgaa 1741 aaccagcatt tctttcaggt gaacagaaga aagccattgt tgatttactc ttcaaaacaa 1801 atcgaaaagt aaccgttaag caattaaaag aagattattt caaaaaaata gaatgttttg 1861 atagtgttga aatttcagga gttgaagata gatttaatgc ttcattaggt acctaccatg 1921 atttgctaaa aattattaaa gataaagatt ttttggataa tgaagaaaat gaagatatct 1981 tagaggatat tgttttaaca ttgaccttat ttgaagatag ggagatgatt gaggaaagac 2041 ttaaaacata tgctcacctc tttgatgata aggtgatgaa acagcttaaa cgtcgccgtt 2101 atactggttg gggacgtttg tctcgaaaat tgattaatgg tattagggat aagcaatctg 2161 gcaaaacaat attagatttt ttgaaatcag atggttttgc caatcgcaat tttatgcagc 2221 tgatccatga tgatagtttg acatttaaag aagacattca aaaagcacaa gtgtctggac 2281 aaggcgatag tttacatgaa catattgcaa atttagctgg tagccctgct attaaaaaag 2341 gtattttaca gactgtaaaa gttgttgatg aattggtcaa agtaatgggg cggcataagc 2401 cagaaaatat cgttattgaa atggcacgtg aaaatcagac aactcaaaag ggccagaaaa 2461 attcgcgaga gcgtatgaaa cgaatcgaag aaggtatcaa agaattagga agtcagattc 2521 ttaaagagca tcctgttgaa aatactcaat tgcaaaatga aaagctctat ctctattatc 2581 tccaaaatgg aagagacatg tatgtggacc aagaattaga tattaatcgt ttaagtgatt 2641 atgatgtcga tcacattgtt ccacaaagtt tccttaaaga cgattcaata gacaataagg 2701 tcttaacgcg ttctgataaa aatcgtggta aatcggataa cgttccaagt gaagaagtag 2761 tcaaaaagat gaaaaactat tggagacaac ttctaaacgc caagttaatc actcaacgta 2821 agtttgataa tttaacgaaa gctgaacgtg gaggtttgag tgaacttgat aaagctggtt 2881 ttatcaaacg ccaattggtt gaaactcgcc aaatcactaa gcatgtggca caaattttgg 2941 atagtcgcat gaatactaaa tacgatgaaa atgataaact tattcgagag gttaaagtga 3001 ttaccttaaa atctaaatta gtttctgact tccgaaaaga tttccaattc tataaagtac 3061 gtgagattaa caattaccat catgcccatg atgcgtatct aaatgccgtc gttggaactg 3121 ctttgattaa gaaatatcca aaacttgaat cggagtttgt ctatggtgat tataaagttt 3181 atgatgttcg taaaatgatt gctaagtctg agcaagaaat aggcaaagca accgcaaaat 3241 atttctttta ctctaatatc atgaacttct tcaaaacaga aattacactt gcaaatggag 3301 agattcgcaa acgccctcta atcgaaacta atggggaaac tggagaaatt gtctgggata 3361 aagggcgaga ttttgccaca gtgcgcaaag tattgtccat gccccaagtc aatattgtca 3421 agaaaacaga agtacagaca ggcggattct ccaaggagtc aattttacca aaaagaaatt 3481 cggacaagct tattgctcgt aaaaaagact gggatccaaa aaaatatggt ggttttgata 3541 gtccaacggt agcttattca gtcctagtgg ttgctaaggt ggaaaaaggg aaatcgaaga 3601 agttaaaatc cgttaaagag ttactaggga tcacaattat ggaaagaagt tcctttgaaa 3661 aaaatccgat tgacttttta gaagctaaag gatataagga agttaaaaaa gacttaatca 3721 ttaaactacc taaatatagt ctttttgagt tagaaaacgg tcgtaaacgg atgctggcta 3781 gtgccggaga attacaaaaa ggaaatgagc tggctctgcc aagcaaatat gtgaattttt 3841 tatatttagc tagtcattat gaaaagttga agggtagtcc agaagataac gaacaaaaac 3901 aattgtttgt ggagcagcat aagcattatt tagatgagat tattgagcaa atcagtgaat 3961 tttctaagcg tgttatttta gcagatgcca atttagataa agttcttagt gcatataaca 4021 aacatagaga caaaccaata cgtgaacaag cagaaaatat tattcattta tttacgttga 4081 cgaatcttgg agctcccgct gcttttaaat attttgatac aacaattgat cgtaaacgat 4141 atacgtctac aaaagaagtt ttagatgcca ctcttatcca tcaatccatc actggtcttt 4201 atgaaacacg cattgatttg agtcagctag gaggtgactg aagtatattt tagatgaaga 4261 ttatttctta ataactaaaa atatggtata atactcttaa taaatgcagt aatacagggg 4321 cttttcaaga ctgaagtcta gctgagacaa atagtgcgat tacgaaattt tttagacaaa 4381 aatagtctac gaggttttag agctatgctg ttttgaatgg tcccaaaact gagaccagtc 4441 tcggaagctc aaaggtctcg ttttagagct atgctgtttt gaatggtccc aaaacttcag 4501 cacactgaga cttgttgagt tccatgtttt agagctatgc tgttttgaat ggactccatt 4561 caacattgcc gatgataact tgagaaagag ggttaatacc agcagtcgga taccttccta 4621 ttctttctgt taaagcgttt tcatgttata ataggcaaaa gaagagtagt gttacgaaat 4681 catcctgtgg agcttagtag gtttagcaag atggcagcgc ctaaatgtag aatgataaaa 4741 ggattaagag attaatttcc ctaaaaatga taaaacaagc gttttgaaag cgcttgtttt 4801 tttggtttgc agtcagagta gaatagaagt atcaaaaaaa gcaccgactc ggtgccactt 4861 tttcaagttg ataacggact agccttattt taacttgcta tgctgttttg aatggttcca 4921 acaagattat tttataactt ttataacaaa taatcaagga gaaattcaaa gaaatttatc 4981 agccataaaa caatacttaa tactatagaa tgataacaaa ataaactact ttttaaaaga 5041 attttgtgtt ataatctatt tattattaag tattgggtaa tattttttga agagatattt 5101 tgaaaaagaa aaattaaagc atattaaact aatttcggag gtcattaaaa ctattattga 5161 aatcatcaaa ctcattatgg atttaattta aactttttat tttaggaggc aaaa //