LOCUS       BBa_K1559011            2584 bp    DNA     linear       22-Dec-2024
DEFINITION  Low-copy plasmid pTRC99A (trc promoter and lacI removed)
ACCESSION   BBa_K1559011
VERSION     BBa_K1559011.1  
FEATURES             Location/Qualifiers
     misc_feature    1..21
                     /label=BioBrick standard suffix
     primer_bind     22..39
                     /label=pBAD rev primer/pTrcHis rev primer
     stem_loop       72..229
                     /label=rrnB terminator
     stem_loop       195..238
                     /label=rrnB T1 terminator
     stem_loop       370..397
                     /label=rrnB t2 terminator
     promoter        439..467
                     /label=AmpR promoter
     CDS             509..1369
                     /label=Ampicillin resistance
     misc_feature    1524..2143
                     /label=pBR322 origin
     primer_bind     2540..2562
                     /label=pGEX 3 primer
     misc_feature    2563..2584
                     /label=BioBrick standard prefix
ORIGIN
        1 tactagtagc ggccgctgca gcctgataca gattaaatca gaacgcagaa gcggtctgat
       61 aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca cctgacccca tgccgaactc
      121 agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct ccccatgcga gagtagggaa
      181 ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
      241 gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg gatttgaacg
      301 ttgcgaagca acggcccgga gggtggcggg caggacgccc gccataaact gccaggcatc
      361 aaattaagca gaaggccatc ctgacggatg gcctttttgc gtttctacaa actctttttg
      421 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      481 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      541 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      601 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      661 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      721 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
      781 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
      841 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
      901 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
      961 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     1021 accaaacgac gagcgtgaca ccacgatgcc tacagcaatg gcaacaacgt tgcgcaaact
     1081 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     1141 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     1201 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     1261 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1321 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1381 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1441 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1501 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1561 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1621 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1681 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1741 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     1801 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     1861 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     1921 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     1981 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     2041 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     2101 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     2161 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     2221 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     2281 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     2341 tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc
     2401 atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga
     2461 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     2521 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga gggaattcgc ggccgcttct
     2581 agag
//