LOCUS       BBa_K1560002            3326 bp    DNA     linear       09-Jan-2025
DEFINITION  Knock-In Knock-Out Vector for HVO_1199
ACCESSION   BBa_K1560002
VERSION     BBa_K1560002.1  
FEATURES             Location/Qualifiers
     misc_feature    18..602
                     /label=hdrB
     misc_feature    608..1138
                     /label=pyrE2
     misc_feature    1141..1263
                     /label=p.fdx promoter
     misc_feature    1503..2288
                     /label=acd3 upstream
     misc_feature    2289..2328
                     /label=KO sequence
     misc_feature    2329..3159
                     /label=acd3 downstream
ORIGIN
        1 ggcaaaatcc cttaagctta ctcatcggat tcctccgcgc cgcggtcagc cgcggcgggc
       61 tgtttgcgct cgtacgtgac gaacgaaaag ccgtcgcggt cgtcgcggtc ggtctcgacc
      121 cacttcccgc ggtcccactc ggggaaccgc gtgtcgcctt cgtgtgccgc gtcgagttcg
      181 gtgagcacca gccccgcggc gcggccgagg aactgctcgt agacggtcgc gccgccgacg
      241 acgtagacgg tctcgacgcc catctcgtcg gccgcgtcct cggcggcggc gaccgccgac
      301 tcgaccgact cgaccgcttc agcgccctcg gggaggtcga ggtcgcgggt cgtcagcacg
      361 acgctgtgac gctccggaag cgggccgccg agttggcggg cgatgctctc gtaggtcttg
      421 cgacccatca cgaccgggtg gccggtcgtc gtccgcttga actgtttcag gtcctcgggg
      481 aggtgccacg gcatgtcgcc gtcgcggccg atgacgcggt tgtcggcgac ggcggcgacg
      541 aggacgaacc ggaccgcggc gtcgccctcg gtttcggacc cagaaagctc ctcgccgctc
      601 atcttgatta gccgtcggcg tcggccagca ggtcctcggc ggtgagaagc gactccagtg
      661 cgatgtcgtg gtcggccagc agctccgccg cgccctcctg tcggtccacg acgacgacga
      721 cgcggtcgac gactgcgccg gcctcgcgga gcgcctccac ggcgtcgacg gcgctctggc
      781 cggtcgtcgc gatgtcttcg aggacgacga cttcctcgcc ctcgtcgagc gcgccctcga
      841 tgcgctttgc cgtcccgtac tcctttgcct tcttgcgggc gatgacgtac ggcttgttcg
      901 tctcgaccgc ggtggcggcc acgagcggga ccgcgccgag cgcgacgccg gccagtttgt
      961 cgtcgtccgc gacgcgctct gcgaacgctt cggcgatgag cttcagacac cgggggtcgg
     1021 tctcgaagag gtacttgtcc acgtagtagc tcgacgtgcc gccgtgggag agctcgaact
     1081 cgccgaactg gaccgcgtcg gcggctcgga gcgcctcgat gagtgctgcg ttcgccatgg
     1141 ctacagagtt cggcttccga acgcatagta gttgctgttc gggccagcgt actgccacga
     1201 aagcccgggt gtgaccgcca tcacgacgct gcccgcggcg tcaacgaggg gttttatcca
     1261 cgggatctaa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     1321 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     1381 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     1441 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     1501 cgcgaaagcc agttgtccgc ctcgagaaat cggatgtgga ggctgtcgcg gtcgtatcgc
     1561 cgagcgagtt cgcgggcgac cgtctccaga cgcggccacc cgaggcggcc gcggacgagc
     1621 cgacggaggt ccatgcaccc ccgtaagacg aggggcggct taacctcgtg ggcctacgac
     1681 gattcctgtc ggaccgtcaa cacgggcgcg tcggagccgc ggacgacgcg ctcggcgacg
     1741 ctcccgatga ggtagcggtc gatgccgctg cggccgtgag ttcccatcac gacgaggtcc
     1801 acctcccgtt cgtcggcgtg gcggataatc tcgctcgcgg gcgtgccctc cgtgacgacg
     1861 gtctcgacct cgacgccggc ctcgcgggcc gcctgagcga cgcggtctag cacctgctca
     1921 ccgctttcga ggagcgcctc gcggacccgt tcgagcgaga gcgcgggcgc ggcgtagccg
     1981 acctcgcgca cgtccgcgac gtagagcgcc acgagcgtcg cgtcgtaggt gcgggcgtgt
     2041 tcgatggcgt ggtccaacgc ggcgtccgcg cagtcgctgc cgtcggtcgg aaagaggatg
     2101 tgggagtaca tacgagaacg tcgggccgcg gcgcaaaaag acccggatgc ctattcccac
     2161 gcgatgggag tacccgacgt gggctgccgc cttcgccggc cgcgacgcat atccttcatg
     2221 gtcgttcgat tagtttatgt ccggcacggc gatagttggt cgcatggact tcgacatccc
     2281 ggccgaacgc gattcaaatg gaccaacatt tagaacgtca caattcttgg acgagcgaga
     2341 ttcaacacct catcatcggc cgcgaactcg gcctctgagc ggccgtcgaa gccggcggtg
     2401 actcactccg actcggccat cgccgacagc cgagcgacgt aggagaaact gcggacgtgg
     2461 tcctcttctg tcggcgcgtc ctcgaagtcg gcggggacgg taaaggagac gacgcgctcg
     2521 cgaatcgtgt cgtgcggctc gtcgcctatc cagccgatat cgcggtagta cgacgccatc
     2581 tccagcgcgc cgcgaccgcc gagtcgctcg acgaggtgga cacaccagtc gagcagcacg
     2641 cgctcggccc gcggcgtccg cggaatctcg tcgaggaacg gctcgccgtc cgaggtgaca
     2701 gccgcggtcg tcgcgaggag gcgctcgtga gccacgtcgc tccacgcccg gttcgtcggt
     2761 gcgtcgaccc agtcgaacga ccgagcgccg gagttccgtc tgagtccggg cccggtcggg
     2821 ccgtcggcgt cagtcgggtg acgtgactcg cgcggcatct atccctgcgt gtgcgtccat
     2881 ccgaattaag cgttcgggcg aacgtccgac atatgacagc cacacgaacg ttcaatccga
     2941 gagagtgggt gaacggttga gctgataact ccgaacgaga ctacgagtcg aaagctgatt
     3001 tgacgggcgt gtcactcggc gtggcgtgat ttctctcttc gctccgtctg ctatcaactt
     3061 tgatatatgg ggcgaatcgc ttaagttccg taccagctac acgtttgata tcgctccgat
     3121 gtgacactca tcggaaagaa cagttccgag agagtcacaa acgtggcgag aaaggaaggg
     3181 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     3241 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg
     3301 ctgcgcaact gttgggaagg gcgatc
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