BBa_K1560002 1 BBa_K1560002 Knock-In Knock-Out Vector for HVO_1199 2014-09-28T11:00:00Z 2015-05-08T01:10:54Z Haloferax Volcanii genomic sequence This part is a non-replicating plasmid designed to knock out the Acd3 gene in Haloferax Volcanii as described here (Allers et al., 2004) false false _1945_ 0 22175 9 In stock false None false Chris Lee, Charles Paine, Max Genetti, Jeff Nivala, David Bernick annotation2392341 1 hdrB range2392341 1 18 602 annotation2392343 1 p.fdx promoter range2392343 1 1141 1263 annotation2392346 1 acd3 downstream range2392346 1 2329 3159 annotation2392344 1 acd3 upstream range2392344 1 1503 2288 annotation2392345 1 KO sequence range2392345 1 2289 2328 annotation2392342 1 pyrE2 range2392342 1 608 1138 BBa_K1560002_sequence 1 ggcaaaatcccttaagcttactcatcggattcctccgcgccgcggtcagccgcggcgggctgtttgcgctcgtacgtgacgaacgaaaagccgtcgcggtcgtcgcggtcggtctcgacccacttcccgcggtcccactcggggaaccgcgtgtcgccttcgtgtgccgcgtcgagttcggtgagcaccagccccgcggcgcggccgaggaactgctcgtagacggtcgcgccgccgacgacgtagacggtctcgacgcccatctcgtcggccgcgtcctcggcggcggcgaccgccgactcgaccgactcgaccgcttcagcgccctcggggaggtcgaggtcgcgggtcgtcagcacgacgctgtgacgctccggaagcgggccgccgagttggcgggcgatgctctcgtaggtcttgcgacccatcacgaccgggtggccggtcgtcgtccgcttgaactgtttcaggtcctcggggaggtgccacggcatgtcgccgtcgcggccgatgacgcggttgtcggcgacggcggcgacgaggacgaaccggaccgcggcgtcgccctcggtttcggacccagaaagctcctcgccgctcatcttgattagccgtcggcgtcggccagcaggtcctcggcggtgagaagcgactccagtgcgatgtcgtggtcggccagcagctccgccgcgccctcctgtcggtccacgacgacgacgacgcggtcgacgactgcgccggcctcgcggagcgcctccacggcgtcgacggcgctctggccggtcgtcgcgatgtcttcgaggacgacgacttcctcgccctcgtcgagcgcgccctcgatgcgctttgccgtcccgtactcctttgccttcttgcgggcgatgacgtacggcttgttcgtctcgaccgcggtggcggccacgagcgggaccgcgccgagcgcgacgccggccagtttgtcgtcgtccgcgacgcgctctgcgaacgcttcggcgatgagcttcagacaccgggggtcggtctcgaagaggtacttgtccacgtagtagctcgacgtgccgccgtgggagagctcgaactcgccgaactggaccgcgtcggcggctcggagcgcctcgatgagtgctgcgttcgccatggctacagagttcggcttccgaacgcatagtagttgctgttcgggccagcgtactgccacgaaagcccgggtgtgaccgccatcacgacgctgcccgcggcgtcaacgaggggttttatccacgggatctaaatcaaaagaatagaccgagatagggttgagtgttgttccagtttggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatcaccctaatcaagttttttggggtcgaggtgccgtaaagcactaaatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgcgaaagccagttgtccgcctcgagaaatcggatgtggaggctgtcgcggtcgtatcgccgagcgagttcgcgggcgaccgtctccagacgcggccacccgaggcggccgcggacgagccgacggaggtccatgcacccccgtaagacgaggggcggcttaacctcgtgggcctacgacgattcctgtcggaccgtcaacacgggcgcgtcggagccgcggacgacgcgctcggcgacgctcccgatgaggtagcggtcgatgccgctgcggccgtgagttcccatcacgacgaggtccacctcccgttcgtcggcgtggcggataatctcgctcgcgggcgtgccctccgtgacgacggtctcgacctcgacgccggcctcgcgggccgcctgagcgacgcggtctagcacctgctcaccgctttcgaggagcgcctcgcggacccgttcgagcgagagcgcgggcgcggcgtagccgacctcgcgcacgtccgcgacgtagagcgccacgagcgtcgcgtcgtaggtgcgggcgtgttcgatggcgtggtccaacgcggcgtccgcgcagtcgctgccgtcggtcggaaagaggatgtgggagtacatacgagaacgtcgggccgcggcgcaaaaagacccggatgcctattcccacgcgatgggagtacccgacgtgggctgccgccttcgccggccgcgacgcatatccttcatggtcgttcgattagtttatgtccggcacggcgatagttggtcgcatggacttcgacatcccggccgaacgcgattcaaatggaccaacatttagaacgtcacaattcttggacgagcgagattcaacacctcatcatcggccgcgaactcggcctctgagcggccgtcgaagccggcggtgactcactccgactcggccatcgccgacagccgagcgacgtaggagaaactgcggacgtggtcctcttctgtcggcgcgtcctcgaagtcggcggggacggtaaaggagacgacgcgctcgcgaatcgtgtcgtgcggctcgtcgcctatccagccgatatcgcggtagtacgacgccatctccagcgcgccgcgaccgccgagtcgctcgacgaggtggacacaccagtcgagcagcacgcgctcggcccgcggcgtccgcggaatctcgtcgaggaacggctcgccgtccgaggtgacagccgcggtcgtcgcgaggaggcgctcgtgagccacgtcgctccacgcccggttcgtcggtgcgtcgacccagtcgaacgaccgagcgccggagttccgtctgagtccgggcccggtcgggccgtcggcgtcagtcgggtgacgtgactcgcgcggcatctatccctgcgtgtgcgtccatccgaattaagcgttcgggcgaacgtccgacatatgacagccacacgaacgttcaatccgagagagtgggtgaacggttgagctgataactccgaacgagactacgagtcgaaagctgatttgacgggcgtgtcactcggcgtggcgtgatttctctcttcgctccgtctgctatcaactttgatatatggggcgaatcgcttaagttccgtaccagctacacgtttgatatcgctccgatgtgacactcatcggaaagaacagttccgagagagtcacaaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgccgcgcttaatgcgccgctacagggcgcgtcccattcgccattcaggctgcgcaactgttgggaagggcgatc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z