BBa_K1560003 1 BBa_K1560003 Knock-In Knock-Out Vector for HVO_1373 2014-09-30T11:00:00Z 2015-05-08T01:10:54Z H. volcanii genomic sequence Composite part consists of part BBa_K156000 (pSCKiKo) with Acd4 knock out insert. false false _1945_ 0 17257 9 In stock false Construct assembled using the Gibson method. Therefore Gibson tagged primers were designed for each sub part. false Chris Lee, Charles Paine, Max Genetti, Jeff Nivala, David Bernick BBa_K1560003_sequence 1 ggcaaaatcccttaagcttactcatcggattcctccgcgccgcggtcagccgcggcgggctgtttgcgctcgtacgtgacgaacgaaaagccgtcgcggtcgtcgcggtcggtctcgacccacttcccgcggtcccactcggggaaccgcgtgtcgccttcgtgtgccgcgtcgagttcggtgagcaccagccccgcggcgcggccgaggaactgctcgtagacggtcgcgccgccgacgacgtagacggtctcgacgcccatctcgtcggccgcgtcctcggcggcggcgaccgccgactcgaccgactcgaccgcttcagcgccctcggggaggtcgaggtcgcgggtcgtcagcacgacgctgtgacgctccggaagcgggccgccgagttggcgggcgatgctctcgtaggtcttgcgacccatcacgaccgggtggccggtcgtcgtccgcttgaactgtttcaggtcctcggggaggtgccacggcatgtcgccgtcgcggccgatgacgcggttgtcggcgacggcggcgacgaggacgaaccggaccgcggcgtcgccctcggtttcggacccagaaagctcctcgccgctcatcttgattagccgtcggcgtcggccagcaggtcctcggcggtgagaagcgactccagtgcgatgtcgtggtcggccagcagctccgccgcgccctcctgtcggtccacgacgacgacgacgcggtcgacgactgcgccggcctcgcggagcgcctccacggcgtcgacggcgctctggccggtcgtcgcgatgtcttcgaggacgacgacttcctcgccctcgtcgagcgcgccctcgatgcgctttgccgtcccgtactcctttgccttcttgcgggcgatgacgtacggcttgttcgtctcgaccgcggtggcggccacgagcgggaccgcgccgagcgcgacgccggccagtttgtcgtcgtccgcgacgcgctctgcgaacgcttcggcgatgagcttcagacaccgggggtcggtctcgaagaggtacttgtccacgtagtagctcgacgtgccgccgtgggagagctcgaactcgccgaactggaccgcgtcggcggctcggagcgcctcgatgagtgctgcgttcgccatggctacagagttcggcttccgaacgcatagtagttgctgttcgggccagcgtactgccacgaaagcccgggtgtgaccgccatcacgacgctgcccgcggcgtcaacgaggggttttatccacgggatctaaatcaaaagaatagaccgagatagggttgagtgttgttccagtttggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatcaccctaatcaagttttttggggtcgaggtgccgtaaagcactaaatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgccgcgcttaatgcgccgctacagggcgcgtcccattcgccattcaggctgcgcaactgttgggaagggcgatcgcttgacggggaaagccggcgggccgaggcgaccgtcatcggcgtcccccacgaacgctggcaggagcgccccgtggcctttatcgtcccgaagggcggcgtcgacgaggacgcgctcaaggccgacctcgtcgcgcgcgtcgaagacgagttcccgaagtggtgggcccccgacgaggtcgtgttcatcgacgaggtgccgaagactgcgaccggcaagttcgacaagaaggtgctccgcgaccggtacgacgactcgtcgctcatcgagggcaagacgcccgattcggaggctccgtcggacgaatgagcgtcgggcaacagctattctaacaaaaaacaagtttgaagtttagactggttccatgcctatttttactgtacgattccatggcgtggtatggaacttctcgacgactccatcgtgcccgaacacgcccgcgacctcaacaagcgataagtccctaactggttgtggccttttgtagagtgaacttcataacatatgctgtctcaggcacgtggatggtttggacaaatcagattcaagcgcaaacgagattcagcgcaacctcatctaccggcagggcgggctgtgagcaggccccggcacggcccccggaactgacaactcgttttccggtttggcatggcttgaccggaacgcagttactatctgagcgtcgtaccttgagtcagcaccaatggtacgcacgagcgttatcggctgcggaaacatggggagcgccctcctcaccgggctctggaaggcgggcggccactcggtcgtcgcctgcgacctcgaccccgacgccctcgccgcggtcgccgactactgtgaagagacgacggacgacgtgtcgcgcgcggccgacgccgacgtggtgttcgtcgcggtcaagcccgacatcgtcgaggccgtcctctcggaactcgatttgtctcccgaccagactctcgtcaccatcgcaacgtggcgagaaaggaagggaagaaagc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z