BBa_K156012 1 Bioplastic phaA (acetyl-CoA acetyltransferase) 2008-10-28T12:00:00Z 2015-05-08T01:10:54Z This coding region is native to ''Ralstonia eutropha'' H16. The original sequence was obtained from GenBank (VERSION NC_008313.1, GI:113866031). This part codes for an enzyme that catalyzes the synthesis of acetoacetyl-coenzyme A from two molecules of acetyl-coenzyme A. The enzyme can also act as a thiolase, catalyzing the reverse reaction and generating two-carbon units from the four-carbon product of fatty acid oxidation. false false _246_ 0 1475 9 It's complicated false This part was codon-optimized for expression in E. coli. The following restriction enzyme sites were also removed: EcoRI, NotI, XbaI, SpeI, PstI, NheI, AvrII, ApoI, MfeI, NsiI, SbfI. false George McArthur IV BBa_K156012_sequence 1 atgactgatgttgttattgtatctgccgctcgtactgctgttggtaaatttggtggtagcctggctaaaatcccggcacctgagctgggcgctgtagttatcaaagctgcgctggaacgtgcgggcgttaaaccggagcaagtgtccgaagtcatcatgggccaagtactgacggcgggcagcggccagaacccggctcgccaggcggccattaaagcaggtctgccggccatggtgccggcaatgaccattaacaaagtttgcggttctggtctgaaagctgttatgctggcagctaatgcaatcatggcaggtgacgcagagattgttgtcgctggtggtcaagaaaacatgagcgctgctccgcatgttctgccaggctcccgtgacggtttccgtatgggtgatgcaaaactggtggacactatgatcgtcgacggcctgtgggatgtgtataaccagtatcacatgggtattaccgcagagaacgttgcgaaagaatatggcattacgcgtgaagcgcaggacgaatttgcagtgggttctcagaacaaagccgaagcggctcagaaagctggtaagttcgacgaagaaatcgtaccggttctgattccgcagcgcaagggtgatccggtagcattcaaaaccgatgagttcgtacgccagggcgctactctggattccatgtctggcctgaaaccggcattcgacaaagcaggtaccgtgacggctgcgaacgcgagcggcctgaacgatggcgcggctgcggttgtggtcatgagcgcggcgaaagctaaagaactgggcctgacgccgctggcgactatcaaatcttacgcgaacgcaggtgtggatccgaaggtgatgggtatgggtcctgtgccagctagcaagcgcgctctgtcccgcgctgaatggactccgcaggatctggatctgatggagattaacgaagctttcgctgcgcaggcgctggcggtacatcagcagatgggttgggacacttctaaagtcaatgtaaacggcggtgccattgcgatcggccacccgatcggcgcttccggttgccgcatcctggtaaccctgctgcacgaaatgaaacgtcgtgacgctaagaaaggtctggcctctctgtgcattggtggcggcatgggtgttgccctggccgttgaacgtaaatga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z