BBa_B0032
1
BBa_B0032
RBS.3 (medium) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1710
1
RBS
range1710
1
7
10
annotation7027
1
BBa_B0032
range7027
1
1
13
annotation1709
1
RBS-3\Weak
range1709
1
1
13
BBa_K156013
1
Bioplastic
phaB1 (acetyacetyl-CoA reductase)
2008-10-28T12:00:00Z
2015-05-08T01:10:54Z
This coding region is native to Ralstonia eutropha H16. The original sequence was obtained from GenBank (VERSION NC_008313.1, GI:113866031).
This part codes for an enzyme that catalyzes the formation of 3-hydroxybutyryl-CoA from acetoacetyl-CoA in polyhydroxyalkanoate synthesis.
false
false
_246_
0
1475
9
It's complicated
false
This part was codon-optimized for expression in E. coli. The following restriction enzyme sites were also removed: EcoRI, NotI, XbaI, SpeI, PstI, NheI, AvrII, ApoI, MfeI, NsiI, SbfI.
false
George McArthur IV
BBa_K156016
1
BBa_K156016
RBS + phaB1
2008-10-28T12:00:00Z
2015-05-08T01:10:54Z
This is a composite part.
This composite part is made of a standard ribosomal binding site and a part that codes for an enzyme that catalyzes the formation of 3-hydroxybutyryl-CoA from acetoacetyl-CoA in polyhydroxyalkanoate synthesis.
false
false
_246_
0
1475
9
It's complicated
true
None.
false
George McArthur IV
component1993477
1
BBa_B0032
component1993479
1
BBa_K156013
annotation1993479
1
BBa_K156013
range1993479
1
20
760
annotation1993477
1
BBa_B0032
range1993477
1
1
13
BBa_K156013_sequence
1
atgactcaacgtatcgcatatgtaactggtggtatgggtggtatcggtactgcaatttgccagcgtctggcgaaagacggtttccgtgttgttgcgggctgcggtccgaactccccgcgtcgtgaaaagtggctggaacaacagaaagccctgggcttcgacttcattgcctccgagggtaatgtagctgactgggattccaccaagactgccttcgataaagttaaatctgaagtgggcgaagtagatgtactgatcaacaacgccggtattactcgtgatgtcgtattccgcaaaatgacccgtgcagactgggatgcagttatcgacaccaacctgacgtctctgttcaacgttaccaaacaggttattgatggtatggctgaccgtggctggggccgcatcgtgaacatctctagcgttaacggccaaaaaggccaatttggtcagacgaattacagcacggctaaagcaggcctgcacggtttcaccatggcactggcgcaggaagtggcgaccaaaggtgttaccgttaataccgtttctccaggttacatcgccaccgatatggttaaggctatccgccaagatgttctggacaagatcgtggctaccattccggttaaacgcctgggcctgccggaagaaattgcgtccatctgtgcgtggctgagctccgaagagtctggtttttccaccggtgcggatttctctctgaacggtggtctgcacatgggttga
BBa_B0032_sequence
1
tcacacaggaaag
BBa_K156016_sequence
1
tcacacaggaaagtactagatgactcaacgtatcgcatatgtaactggtggtatgggtggtatcggtactgcaatttgccagcgtctggcgaaagacggtttccgtgttgttgcgggctgcggtccgaactccccgcgtcgtgaaaagtggctggaacaacagaaagccctgggcttcgacttcattgcctccgagggtaatgtagctgactgggattccaccaagactgccttcgataaagttaaatctgaagtgggcgaagtagatgtactgatcaacaacgccggtattactcgtgatgtcgtattccgcaaaatgacccgtgcagactgggatgcagttatcgacaccaacctgacgtctctgttcaacgttaccaaacaggttattgatggtatggctgaccgtggctggggccgcatcgtgaacatctctagcgttaacggccaaaaaggccaatttggtcagacgaattacagcacggctaaagcaggcctgcacggtttcaccatggcactggcgcaggaagtggcgaccaaaggtgttaccgttaataccgtttctccaggttacatcgccaccgatatggttaaggctatccgccaagatgttctggacaagatcgtggctaccattccggttaaacgcctgggcctgccggaagaaattgcgtccatctgtgcgtggctgagctccgaagagtctggtttttccaccggtgcggatttctctctgaacggtggtctgcacatgggttga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z