BBa_K156020
1
BBa_K156020
Promoter + RBS + phaC1
2008-10-28T12:00:00Z
2015-05-08T01:10:54Z
This is a composite part.
This composite part is made of a promoter, a standard ribosomal binding site and a part that codes for a polyhydroxybutyrate (PHB) synthase, which catalyzes the polymerization of hydroxybutyryl-CoA.
false
false
_246_
0
1475
9
Not in stock
false
None.
false
George McArthur IV
component1993683
1
BBa_K156014
component1993681
1
BBa_B0032
component1993675
1
BBa_R0040
annotation1993683
1
BBa_K156014
range1993683
1
82
1430
annotation1993681
1
BBa_B0032
range1993681
1
63
75
annotation1993675
1
BBa_R0040
range1993675
1
1
54
BBa_R0040
1
p(tetR)
TetR repressible promoter
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210.
Released HQ 2013
Sequence for pTet inverting regulator driven by the TetR protein.</P>
false
true
_1_
0
24
7
In stock
false
<P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P>
true
June Rhee, Connie Tao, Ty Thomson, Louis Waldman
annotation1986785
1
-35
range1986785
1
20
25
annotation1986783
1
TetR 1
range1986783
1
1
19
annotation1986784
1
BBa_R0040
range1986784
1
1
54
annotation1986787
1
-10
range1986787
1
43
48
annotation1986786
1
TetR 2
range1986786
1
26
44
BBa_B0032
1
BBa_B0032
RBS.3 (medium) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1709
1
RBS-3\Weak
range1709
1
1
13
annotation1710
1
RBS
range1710
1
7
10
annotation7027
1
BBa_B0032
range7027
1
1
13
BBa_K156014
1
Bioplastic
phaC1 (Poly(3-hydroxybutyrate) polymerase)
2008-10-28T12:00:00Z
2015-05-08T01:10:54Z
This coding region is native to Ralstonia eutropha H16. The original sequence was obtained from GenBank (VERSION NC_008313.1, GI:113866031).
This part codes for a polyhydroxybutyrate (PHB) synthase, which catalyzes the polymerization of hydroxybutyryl-CoA.
false
false
_246_
0
1475
9
It's complicated
false
This part was codon-optimized for expression in E. coli. The following restriction enzyme sites were also removed: EcoRI, NotI, XbaI, SpeI, PstI, NheI, AvrII, ApoI, MfeI, NsiI, SbfI.
false
George McArthur IV
BBa_K156020_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacacaggaaagtactagatggcaactggtaaaggtgctgctgcatctactcaagaaggtaaatcccagccgttcaaagtgacccctggtccgttcgatccggcgacctggctggaatggagccgccagtggcagggcactgaaggcaacggccacgcagctgcttctggcattccgggtctggacgctctggcgggcgtaaaaatcgcaccggcacaactgggcgacatccagcaacgttatatgaaagatttcagcgcgctgtggcaggcgatggcggaaggcaaagccgaagcgaccggtccgctgcacgaccgtcgtttcgcaggtgacgcgtggcgtaccaacctgccataccgtttcgctgccgccttttacctgctgaacgcacgtgcgctgaccgaactggctgatgcagttgaagctgacgccaaaacccgccagcgtatccgtttcgctatcagccagtgggtcgacgcgatgagcccggccaacttcctggccacgaacccggaggcccagcgcctgctgatcgaatctggtggtgaatctctgcgtgcgggcgttcgtaacatgatggaggatctgacgcgcggtaagatctctcaaaccgacgagagcgcgtttgaagttggccgtaacgtggcggttactgaaggcgcggttgttttcgagaacgaatatttccagctgctgcaatacaaaccgctgaccgacaaggttcacgctcgtccgctgctgatggtaccgccttgcatcaacaaatactacatcctggacctgcaaccagaatcctctctggttcgtcacgtggttgagcaaggtcacaccgtgttcctggtttcctggcgcaacccggatgcttctatggcaggttctacgtgggatgattacatcgagcacgcggctattcgtgcgatcgaagttgcacgtgatattagcggccaggataagatcaacgtactgggcttttgcgttggcggtactattgttagcactgcactggcagttctggcggcacgtggcgagcatccggctgcatccgttaccctgctgaccaccctgctggattttgcggacactggcatcctggacgtctttgttgatgaaggtcacgtgcagctgcgcgaagcgactctgggtggtggcgcgggtgctccgtgcgcactgctgcgcggcctggaactggctaacactttctcctttctgcgtccgaacgacctggtgtggaactacgttgtggacaactacctgaaaggtaacacgccggtgcctttcgatctgctgttctggaacggcgatgctaccaatctgccgggtccatggtattgctggtacctgcgtcacacttatctgcaaaacgaact
BBa_R0040_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
BBa_K156014_sequence
1
atggcaactggtaaaggtgctgctgcatctactcaagaaggtaaatcccagccgttcaaagtgacccctggtccgttcgatccggcgacctggctggaatggagccgccagtggcagggcactgaaggcaacggccacgcagctgcttctggcattccgggtctggacgctctggcgggcgtaaaaatcgcaccggcacaactgggcgacatccagcaacgttatatgaaagatttcagcgcgctgtggcaggcgatggcggaaggcaaagccgaagcgaccggtccgctgcacgaccgtcgtttcgcaggtgacgcgtggcgtaccaacctgccataccgtttcgctgccgccttttacctgctgaacgcacgtgcgctgaccgaactggctgatgcagttgaagctgacgccaaaacccgccagcgtatccgtttcgctatcagccagtgggtcgacgcgatgagcccggccaacttcctggccacgaacccggaggcccagcgcctgctgatcgaatctggtggtgaatctctgcgtgcgggcgttcgtaacatgatggaggatctgacgcgcggtaagatctctcaaaccgacgagagcgcgtttgaagttggccgtaacgtggcggttactgaaggcgcggttgttttcgagaacgaatatttccagctgctgcaatacaaaccgctgaccgacaaggttcacgctcgtccgctgctgatggtaccgccttgcatcaacaaatactacatcctggacctgcaaccagaatcctctctggttcgtcacgtggttgagcaaggtcacaccgtgttcctggtttcctggcgcaacccggatgcttctatggcaggttctacgtgggatgattacatcgagcacgcggctattcgtgcgatcgaagttgcacgtgatattagcggccaggataagatcaacgtactgggcttttgcgttggcggtactattgttagcactgcactggcagttctggcggcacgtggcgagcatccggctgcatccgttaccctgctgaccaccctgctggattttgcggacactggcatcctggacgtctttgttgatgaaggtcacgtgcagctgcgcgaagcgactctgggtggtggcgcgggtgctccgtgcgcactgctgcgcggcctggaactggctaacactttctcctttctgcgtccgaacgacctggtgtggaactacgttgtggacaactacctgaaaggtaacacgccggtgcctttcgatctgctgttctggaacggcgatgctaccaatctgccgggtccatggtattgctggtacctgcgtcacacttatctgcaaaacgaact
BBa_B0032_sequence
1
tcacacaggaaag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z