LOCUS       BBa_K156021             1400 bp    DNA     linear       26-Dec-2024
DEFINITION  Promoter + RBS + phaA + double terminator
ACCESSION   BBa_K156021
VERSION     BBa_K156021.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..75
                     /label=RBS-3\Weak
     RBS             69..72
                     /label=RBS
     misc_feature    63..75
                     /label=BBa_B0032
     RBS             63..75
                     /label=BBa_B0032
     CDS             82..1263
                     /label=BBa_K156012
     stem_loop       1283..1326
                     /label=stem_loop
     misc_feature    1272..1351
                     /label=BBa_B0010
     terminator      1272..1351
                     /label=BBa_B0010
     stem_loop       1367..1386
                     /label=T7 TE
     misc_feature    1393..1393
                     /label=stop
     polyA_site      1387..1400
                     /label=polya
     misc_feature    1360..1400
                     /label=BBa_B0012
     terminator      1360..1400
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agtcacacag gaaagtacta gatgactgat gttgttattg tatctgccgc tcgtactgct
      121 gttggtaaat ttggtggtag cctggctaaa atcccggcac ctgagctggg cgctgtagtt
      181 atcaaagctg cgctggaacg tgcgggcgtt aaaccggagc aagtgtccga agtcatcatg
      241 ggccaagtac tgacggcggg cagcggccag aacccggctc gccaggcggc cattaaagca
      301 ggtctgccgg ccatggtgcc ggcaatgacc attaacaaag tttgcggttc tggtctgaaa
      361 gctgttatgc tggcagctaa tgcaatcatg gcaggtgacg cagagattgt tgtcgctggt
      421 ggtcaagaaa acatgagcgc tgctccgcat gttctgccag gctcccgtga cggtttccgt
      481 atgggtgatg caaaactggt ggacactatg atcgtcgacg gcctgtggga tgtgtataac
      541 cagtatcaca tgggtattac cgcagagaac gttgcgaaag aatatggcat tacgcgtgaa
      601 gcgcaggacg aatttgcagt gggttctcag aacaaagccg aagcggctca gaaagctggt
      661 aagttcgacg aagaaatcgt accggttctg attccgcagc gcaagggtga tccggtagca
      721 ttcaaaaccg atgagttcgt acgccagggc gctactctgg attccatgtc tggcctgaaa
      781 ccggcattcg acaaagcagg taccgtgacg gctgcgaacg cgagcggcct gaacgatggc
      841 gcggctgcgg ttgtggtcat gagcgcggcg aaagctaaag aactgggcct gacgccgctg
      901 gcgactatca aatcttacgc gaacgcaggt gtggatccga aggtgatggg tatgggtcct
      961 gtgccagcta gcaagcgcgc tctgtcccgc gctgaatgga ctccgcagga tctggatctg
     1021 atggagatta acgaagcttt cgctgcgcag gcgctggcgg tacatcagca gatgggttgg
     1081 gacacttcta aagtcaatgt aaacggcggt gccattgcga tcggccaccc gatcggcgct
     1141 tccggttgcc gcatcctggt aaccctgctg cacgaaatga aacgtcgtga cgctaagaaa
     1201 ggtctggcct ctctgtgcat tggtggcggc atgggtgttg ccctggccgt tgaacgtaaa
     1261 tgatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     1321 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
     1381 tgggcctttc tgcgtttata
//