##gff-version 3
##sequence-region BBa_K156021 1 1400
BBa_K156021	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K156021	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K156021	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K156021	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K156021	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K156021	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K156021	.	ribosome_entry_site	63	75	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K156021	.	engineered_region	63	75	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K156021	.	ribosome_entry_site	69	72	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K156021	.	ribosome_entry_site	63	75	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K156021	.	terminator	1360	1400	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K156021	.	stop_codon	1393	1393	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K156021	.	engineered_region	1360	1400	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K156021	.	polyA_site	1387	1400	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K156021	.	stem_loop	1367	1386	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K156021	.	CDS	82	1263	.	+	0	ID=BBa_K156012;Name=BBa_K156012
BBa_K156021	.	terminator	1272	1351	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K156021	.	engineered_region	1272	1351	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K156021	.	stem_loop	1283	1326	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_K156021
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacacaggaaagtact
agatgactgatgttgttattgtatctgccgctcgtactgctgttggtaaatttggtggtagcctggctaaaatcccggc
acctgagctgggcgctgtagttatcaaagctgcgctggaacgtgcgggcgttaaaccggagcaagtgtccgaagtcatc
atgggccaagtactgacggcgggcagcggccagaacccggctcgccaggcggccattaaagcaggtctgccggccatgg
tgccggcaatgaccattaacaaagtttgcggttctggtctgaaagctgttatgctggcagctaatgcaatcatggcagg
tgacgcagagattgttgtcgctggtggtcaagaaaacatgagcgctgctccgcatgttctgccaggctcccgtgacggt
ttccgtatgggtgatgcaaaactggtggacactatgatcgtcgacggcctgtgggatgtgtataaccagtatcacatgg
gtattaccgcagagaacgttgcgaaagaatatggcattacgcgtgaagcgcaggacgaatttgcagtgggttctcagaa
caaagccgaagcggctcagaaagctggtaagttcgacgaagaaatcgtaccggttctgattccgcagcgcaagggtgat
ccggtagcattcaaaaccgatgagttcgtacgccagggcgctactctggattccatgtctggcctgaaaccggcattcg
acaaagcaggtaccgtgacggctgcgaacgcgagcggcctgaacgatggcgcggctgcggttgtggtcatgagcgcggc
gaaagctaaagaactgggcctgacgccgctggcgactatcaaatcttacgcgaacgcaggtgtggatccgaaggtgatg
ggtatgggtcctgtgccagctagcaagcgcgctctgtcccgcgctgaatggactccgcaggatctggatctgatggaga
ttaacgaagctttcgctgcgcaggcgctggcggtacatcagcagatgggttgggacacttctaaagtcaatgtaaacgg
cggtgccattgcgatcggccacccgatcggcgcttccggttgccgcatcctggtaaccctgctgcacgaaatgaaacgt
cgtgacgctaagaaaggtctggcctctctgtgcattggtggcggcatgggtgttgccctggccgttgaacgtaaatgat
actagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtga
acgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata